LOCUS Exported 7044 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION P. aeruginosa PA14 CRISPR2 locus, with ""optimized"" IHFprox site. ACCESSION . VERSION . KEYWORDS pCRISPR2_IHF_Opt SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7044) TITLE Phasing paper plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 7044) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7044 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 37..59 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" primer_bind complement(1994..2011) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(1994..2010) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2003..2025) /label=M13/pUC Forward /note="In lacZ gene" CDS complement(2171..3004) /codon_start=1 /product="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa " /label=pRO1600 Rep /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA" rep_origin 3018..3369 /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" promoter 3410..3438 /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" rep_origin 4942..5530 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5431..5450 /label=pBR322ori-F /note="pBR322 origin, forward primer" oriT 5600..5708 /note="incP origin of transfer" CDS complement(5865..6743) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 6770..10 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 6977..6996 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 gtttctccat acccgttttt ttgggctaga aataattttg tttaacttta agaaggagat 61 atacataccc atggatgcct tgtccgaaat cgaggcgggc cacccgagcc tgtagcgccg 121 ctccctggcg cgacgccccc ggcctggccg ggggctttgc ggcatcgccc atcaaaagac 181 ctttcgcgcc cgaacggcac ggttgatcgc cgtcccggtc ctcgcgaaac ggccgccaat 241 tgcccgaagc ttccgaccct tttttcggac gatttcttac gcccttataa atcagcaagt 301 tacgagacct cgaaaaaaga gggtttctgg cgggaaaaac tcaaaaaacg cttcgaaatc 361 aaccggttat aggttttcgg agctagttca ctgccgtgta ggcagctaag aaaatcagcc 421 ggacgttgta gtagtcgagc gcggtgttca ctgccgtgta ggcagctaag aaagccgggt 481 ggtgacttct ggacggcgtt cgtcaagttc actgccgtgt aggcagctaa gaaagacctg 541 gttttggccg acacagtggc tgcgacgttc actgccgtgt aggcagctaa gaaacggtcc 601 tgccagtact gcggcaggat ctggtcgttc actgccgtgt aggcagctaa gaaaagcctg 661 gcgcccttcg agtccatcca gcccgggttc actgccgtgt aggcagctaa gaaacatcca 721 attcgccgat ccggcgcaag cgcccggttc actgccgtgt aggcagctaa gaaaacgaag 781 cagatcgccg aatgcggcgt cagcgcgttc actgccgtgt aggcagctaa gaaagtgggt 841 gcaaatcgcg attctggtcg cgtcgtgttc actgccgtgt aggcagctaa gaaatgaata 901 tcagcctgca aagcgtcgtc gtagtagttc actgccgtgt aggcagctaa gaaaaagccg 961 ggccgatgtt cgagcctggc tgcgtggttc actgccgtgt aggcagctaa gaaatggaca 1021 atgcgcagcc caggcgctgg atttacgttc actgccgtgt aggcagctaa gaaaatgcga 1081 ggctgatcga ggtgcgcggt tctgttgttc actgccgtgt aggcagctaa gaaagtgcgc 1141 ggatggctgg tatctcgagc acgattgttc actgccgtgt aggcagctaa gaaaaggcaa 1201 acaacggaaa gatcaccacc gaaggcgttc actgccgtgt aggcagctaa gaaaaacgaa 1261 acaggcccag cacagccgct tgataagttc actgccgtgt aggcagctaa gaaaacaaga 1321 cgctcatcga cggcagcatc gagacggttc actgccgtgt aggcagctaa gaaaaaggtg 1381 acgatgcaca gctgttgcgc gcggttgttc actgccgtgt aggcagctaa gaaaatcctc 1441 gaacagcccg ggttcggtcg cctccagttc actgccgtgt aggcagctaa gaaaaggagt 1501 ttgcggcccc gctccctgcg gctctcgttc actgccgtgt aggcagctaa gaaattcacg 1561 gcgggcttga tgtccgcgtc tacctggttc actgccgtgt aggcagctaa gaaattgccg 1621 agtacgatgc ctgatacatg aatccagttc actgccgtgt aggcagctaa gaaactcgaa 1681 cccacctcgg ccacaacagc cgccgggttc gctgccgtct aggcagaacc accctcccca 1741 tcccactacc aaacatccga atataaagtt cctaccccgc ccgccagcct cgcccgtcca 1801 cgacaatgtg cctcgcttgg aagctcacgc tcctcacaca gacgaaaacg gggtagcggc 1861 aatcggccat atccgctaaa cagttgcctt ggcgcagaat tcgatagatc cgatagggac 1921 aggccacggt caacatggac gacatttctc ccagcgaact gaagacgatc cttcactcgc 1981 atgcaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 2041 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 2101 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa aggcaggccg ggccgtggtg 2161 gccacggcct ctaggccaga tccagcggca tctgggttag tcgagcgcgg gccgcttccc 2221 atgtctcacc agggcgagcc tgtttcgcga tctcagcatc tgaaatcttc ccggccttgc 2281 gcttcgctgg ggccttaccc accgccttgg cgggcttctt cggtccaaaa ctgaacaaca 2341 gatgtgtgac cttgcgcccg gtctttcgct gcgcccactc cacctgtagc gggctgtgct 2401 cgttgatctg cgtcacggct ggatcaagca ctcgcaactt gaagtccttg atcgagggat 2461 accggccttc cagttgaaac cactttcgca gctggtcaat ttctatttcg cgctggccga 2521 tgctgtccca ttgcatgagc agctcgtaaa gcctgatcgc gtgggtgctg tccatcttgg 2581 ccacgtcagc caaggcgtat ttggtgaact gtttggtgag ttccgtcagg tacggcagca 2641 tgtctttggt gaacctgagt tctacacggc cctcaccctc ccggtagatg attgtttgca 2701 cccagccggt aatcatcaca ctcggtcttt tccccttgcc attgggctct tgggttaacc 2761 ggacttcccg ccgtttcagg cgcagggccg cttctttgag ctggttgtag gaagattcga 2821 tagggacacc cgccatcgtc gctatgtcct ccgccgtcac tgaatacatc acttcatcgg 2881 tgacaggctc gctcctcttc acctggctaa tacaggccag aacgatccgc tgttcctgaa 2941 cactgaggcg atacgcggcc tcgaccaggg cattgctttt gtaaaccatt gggggtgagg 3001 ccacgttcga cattccttgt gtataagggg acactgtatc tgcgtcccac aatacaacaa 3061 atccgtccct ttacaacaac aaatccgtcc cttcttaaca acaaatccgt cccttaatgg 3121 caacaaatcc gtcccttttt aaactctaca ggccacggat tacgtggcct gtagacgtcc 3181 taaaaggttt aaaagggaaa aggaagaaaa gggtggaaac gcaaaaaacg caccactacg 3241 tggccccgtt ggggccgcat ttgtgcccct gaaggggcgg gggaggcgtc tgggcaatcc 3301 ccgttttacc agtcccctat cgccgcctga gagggcgcag gaagcgagta atcagggtat 3361 cgaggcggat tcacccttgg cgtccaacca gcggcaccag cggcgcgaat tgacataagc 3421 ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac 3481 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg 3541 tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg 3601 atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa 3661 gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg accaagtcaa 3721 atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag ccacctactc 3781 ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga cattcatcgc 3841 gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg ttctgcccaa 3901 gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg gcgagcaccg 3961 gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca acgcgcttgg 4021 tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg ctctctatac 4081 aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta ccgccaccta 4141 acaattcgtt caagccgaga tcggcttccc ggccgcggag ttgttcggta aattgtcaca 4201 acgccgcggc caattcgact caccagtcac agaaaagcat cttacggatg gcatgacagt 4261 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 4321 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 4381 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 4441 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 4501 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 4561 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 4621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 4681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 4741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 4801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 4861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 4921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 4981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 5041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 5101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 5161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 5221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 5281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 5341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 5401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 5461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 5521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 5581 tgctcacaag ctagcttatc ggccagcctc gcagagcagg attcccgttg agcaccgcca 5641 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta 5701 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa 5761 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga 5821 agcagggtta tgcagcggaa agtatacctt aaggaatccc caaattatga caacttgacg 5881 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 5941 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 6001 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 6061 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac 6121 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 6181 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 6241 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc 6301 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 6361 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 6421 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 6481 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 6541 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 6601 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 6661 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 6721 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 6781 tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 6841 aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 6901 aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 6961 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga 7021 cgctttttat cgcaactctc tact //