LOCUS Exported 9054 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS HA-Sos2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9054) AUTHORS Yang SS, Van Aelst L, Bar-Sagi D TITLE Differential interactions of human Sos1 and Sos2 with Grb2. JOURNAL J Biol Chem. 1995 Aug 4;270(31):18212-5. PUBMED 7629138 REFERENCE 2 (bases 1 to 9054) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9054 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 304..507 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 458..478 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 504..528 /label=LNCX /note="Human CMV promoter, forward primer" 5'UTR 597..636 /label=HSV TK 5'-UTR /note="5' untranslated region from the herpes simplex virus thymidine kinase gene" CDS 666..692 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" intron 4752..5324 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind complement(5456..5475) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 5521..5576 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5575..5594) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" rep_origin 5930..6065 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5984..6003) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6171..6188) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6342..6930) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6422..6441) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7101..7961) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7724..7743 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7962..8066) /gene="bla" /label=AmpR promoter primer_bind 8134..8152 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8190..8212) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8312..8331 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(8398..8853) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8535..8556) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8747..8766 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8979..9001 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 8993..9010 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 8994..9010 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 9017..9036 /label=T7 /note="T7 promoter, forward primer" promoter 9017..9035 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" enhancer 9054..303 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc 541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc 601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt 661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agtggatcca 721 ccatgcagca ggcgccgcag ccttacgagt tcttcagcga ggagaacagt ccgaaatggc 781 ggggactgtt ggtctcggcc ctgcggaagg ttcaggaaca agtgcatccc actctctcag 841 ctaatgaaga gtctctctat tatattgaag agctgatttt tcagctgctt aataaattat 901 gcatggccca gccaaggact gttcaagatg tagaggagcg agttcagaag acctttcctc 961 acccaattga taaatgggcc attgctgatg cacaatctgc tatagaaaaa cgaaaacgaa 1021 gaaatcctct tttactgcct gtggacaaaa tccatccttc gttgaaggaa gtattagggt 1081 acaaagtgga ctaccatgta tccctatata ttgtggctgt actagagtat atctcagctg 1141 atattttaaa attggctggt aattatgttt ttaatatccg gcattatgaa atatctcagc 1201 aggacattaa agtgtcaatg tgtgcggata aggttttgat ggacatgttt gatcaggatg 1261 acataggttt ggtttctctc tgtgaagatg aacctagttc ttctggtgaa ttaaactact 1321 atgatcttgt cagaactgaa atcgcagaag aaagacagta tctacgggaa ttaaatatga 1381 tcataaaagt gtttcgagaa gcctttcttt ctgatagaaa gctgtttaaa ccttctgata 1441 tcgaaaagat ttttagtaac atttcagata tacatgaatt gactgtgaaa cttttaggtt 1501 tgattgaaga cacagttgaa atgactgatg aaagcagtcc tcatccctta gctggcagct 1561 gttttgaaga tttggcagaa gagcaagcat ttgatcctta tgaaacatta tcacaggaca 1621 ttctttcacc agagtttcat gaacatttca ataaattgat ggccagacct gcagttgctc 1681 tacactttca gtccattgct gatggtttta aagaggcagt tcgttatgtc cttccacgtc 1741 ttatgctggt gccagtgtat cactgttggc actactttga gttactaaag caattgaaag 1801 catgtagtga agaacaagaa gacagagaat gtttgaacca agctattact gctctcatga 1861 atctccaagg tagcatggac cgaatttaca agcagtattc acctagacgt cgacctggag 1921 atcctgtttg ccctttttat agtcaccaat taagaagcaa acacctggct atcaaaaaaa 1981 tgaatgaaat tcagaaaaat atcgatggat gggaaggcaa agatattgga cagtgttgta 2041 atgaattcat tatggaggga ccattgacaa gaatcggtgc caaacatgaa cggcatattt 2101 ttctgtttga tggcttaatg atcagttgta aacctaatca tggccagact cggcttccag 2161 gttacagtag tgcagaatac aggttaaaag aaaaatttgt catgaggaaa atacaaattt 2221 gtgataaaga agatacttgt gagcacaagc atgcatttga attagtatcc aaagatgaga 2281 acagcataat atttgctgct aagtctgctg aagaaaaaaa caactggatg gcagccctta 2341 tttctcttca ttatcgtagt actctagatc gaatgttaga ttcagtatta ttgaaagaag 2401 aaaatgagca accactgaga ttaccaagtc ctgaagtata tcgttttgta gtaaaagact 2461 ctgaggaaaa cattgttttt gaagacaact tgcaaagtag aagtggcatc cccattatta 2521 aaggaggaac tgtagtgaaa ttaattgaaa ggttaacata tcatatgtat gcagatccca 2581 attttgttcg tacttttctt accacatatc gttcattttg taaaccacag gaattgctga 2641 gcttactgat tgaacggttt gaaattccag agccagaacc tactgacgca gacaaattgg 2701 caatagagaa aggcgagcag ccaatcagtg cagaccttaa aagatttcgc aaggaatatg 2761 tccaaccagt acaacttagg atcttaaatg tatttcggca ttgggttgaa catcattttt 2821 atgactttga aagagacttg gaattgcttg aaagactaga atccttcatt tcaagtgtaa 2881 gagggaaagc tatgaaaaaa tgggtagagt caattgctaa gatcatcagg aggaagaagc 2941 aagctcaggc aaatggagta agccataata ttacctttga aagtccacct ccaccaattg 3001 aatggcatat cagcaaacca ggacagtttg aaacatttga tctcatgaca cttcatccaa 3061 tagaaattgc acgtcagctg acacttttgg agtctgatct ttacaggaaa gttcaaccgt 3121 ctgaacttgt agggagtgtg tggaccaaag aagataaaga aataaattct ccaaatttat 3181 taaaaatgat tcgccatacc acaaatctca ccctctggtt tgaaaaatgc attgtggaag 3241 cagaaaattt tgaagaacgg gtggcagtac taagtagaat tatagaaatt ctgcaagttt 3301 ttcaagattt gaataatttc aatggcgtat tggagatagt cagtgcagta aattcagtgt 3361 cagtatacag actagaccat acctttgagg cactgcagga aaggaaaagg aaaattttgg 3421 acgaagctgt ggaattaagt caagatcact ttaaaaaata cctagtaaaa cttaagtcaa 3481 tcaatccacc ttgtgtgcct ttttttggaa tatatttaac aaatattctg aagaccgaag 3541 aagggaataa tgatttttta aaaaagaaag ggaaagattt aatcaatttc agtaagagga 3601 ggaaagtagc tgaaattact ggagaaattc agcagtatca gaatcagcct tactgtttac 3661 ggatagaacc agatatgagg agattctttg aaaaccttaa ccccatggga agtgcatctg 3721 aaaaagagtt tacagattat ttgttcaaca agtcactaga aattgaacct cgaaactgca 3781 aacagccacc tcgatttcct aggaaatcaa ctttttcctt aaaatctcct ggaataaggc 3841 ctaacacagg ccgacatggc tctacctcag gtactttacg aggtcaccca acaccattag 3901 aaagagaacc atgtaaaata agctttagtc ggattgctga aactgagctg gaatcaacag 3961 tgtcagcacc aacctctcca aatacaccat ctactccacc agtatctgct tcttcagacc 4021 ttagtgtatt tttagatgtg gatctcaaca gctcctgtgg cagcaatagc atctttgctc 4081 cagtgctttt gccacattca aagtctttct ttagttcatg tggtagttta cataaactaa 4141 gtgaagagcc cctgattcct cctcctcttc ctcctcgaaa aaagtttgat catgatgctt 4201 caaattccaa gggaaatatg aaatctgatg atgatcctcc tgctattcca ccgagacagc 4261 ctcctcctcc aaaggtaaaa cccagagttc ctgttcctac tggtgcattt gatgggcctc 4321 tgcatagtcc acctccgcca ccaccaagag atcctcttcc tgatacccct ccaccagttc 4381 cccttcggcc tccagaacac tttataaact gtccatttaa tcttcagcca cctccactgg 4441 ggcatcttca cagagattca gactggctca gagacattag tacgtgtcca aattcgccaa 4501 gcactcctcc tagcacaccc tctccaaggg taccgcgtcg atgctatgtg ctcagttcta 4561 gtcagaataa tcttgctcat cctccagctc cccctgttcc accaaggcag aattcaagcc 4621 ctcatctgcc aaaactgcca ccaaagactt acaaacggga gctttcgcac cccccattgt 4681 acagactgcc tttgctagaa aatgcagaaa ctccccaatg acccggatcc cccgatcctg 4741 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat 4801 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct 4861 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac 4921 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc 4981 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct 5041 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga 5101 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc 5161 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt 5221 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg 5281 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc 5341 tggttgttgt gctgtctcat cattttggca aagaattcac tcctcaggtg caggctgcct 5401 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt 5461 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct 5521 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg 5581 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt 5641 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc 5701 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag 5761 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt 5821 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg 5881 tccctcttct cttatggaga tccctcgagg agctttttgc aaaagcctag gcctccaaaa 5941 aagcctcttc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa 6001 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc 6061 gggatgggcg gagttagggg cgggactatg gttgctgact aattgctgca ttaatgaatc 6121 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 6181 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 6241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 6301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 6361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 6421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 6481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 6541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 6601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 6661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 6721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 6781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 7021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 7081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 7141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 7201 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 7261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 7321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 7381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 7441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 7501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 7561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 7621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 7681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 7741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 7801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 7861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 8041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 8101 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 8161 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 8221 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 8281 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 8341 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa 8401 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 8461 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 8521 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 8581 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 8641 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 8701 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 8761 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 8821 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg 8881 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 8941 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 9001 gacggccagt gaattgtaat acgactcact atagggcgaa ttcgagctcg cccc //