LOCUS Exported 6395 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter. ACCESSION . VERSION . KEYWORDS pAM-AAV-mSyn1-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6395) AUTHORS Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L, Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, Goldberg JL, Hu Y TITLE Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells. JOURNAL J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 10.1523/JNEUROSCI.0102-20.2020. PUBMED 32300046 REFERENCE 2 (bases 1 to 6395) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6395 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 100..118 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 100..117 /label=SP6 /note="SP6 promoter, forward primer" repeat_region 139..268 /label=AAV2 ITR CDS 1576..2295 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 1576..2292 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1621..1642) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1882..1901) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2229..2250 /label=EGFP-C /note="EGFP, forward primer" misc_feature 2353..2941 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2406..2426) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2824..2835) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2968..2985) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 2974..3198 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter complement(3408..3737) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3422..3557 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(3476..3495) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 3722..3742 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" primer_bind complement(3812..3829) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3983..4571) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4063..4082) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4742..5602) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5365..5384 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5603..5707) /gene="bla" /label=AmpR promoter primer_bind 5775..5793 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(5831..5853) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 5953..5972 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6186..6205 /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" ORIGIN 1 ctgcttcagt aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg 61 ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac actatagaat 121 acacggaatt aattctagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc 181 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga 241 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta 301 tctacgtagc catgctctag gtaccattga cgtcaataat gacgtatgtt cccatagtaa 361 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact 421 tggcagtaca tcaagtgtat caccatatgt gagccttctt attcagacca ccaaaattac 481 tttatttttc cacatgaaag tattatgtgg cttcctgtct gcaaagagga agacatccat 541 gaacactaat gacactgggt ttgggctatg tccggagcag aggaatgagg ccatgtagac 601 taaatatgtg catgtggagg aggctgaaaa cacatcagag ctagcgctgc aggaaatgct 661 tctgcattgc atacccagag tttccttgct catctgggag tctgtgtttt tcctagatgt 721 gtgcacttgt gtgagattct ctgggtgtga gtcaaagtgt tatctgaatg tgtaatgtgt 781 gctcaatatg ctcatgtgtg ttaccctgag cttctgtgtc tacatatata cctggatgcc 841 tgtgtgttct gtgatgtaca tatatattct gtctttcctt ccttttctat ttgtgttatt 901 ccatgtgttc tttcagattc tcaccaccaa gggcaaggat atgttaacta cccaagtgtc 961 cacctccgcc tgtctggtga tgtttacgcc acccccgtgc tcttttcttt gcccgacaga 1021 gttgttatag gagatgtctc cccgggaaca ctgcaggaag gagaatttct acatttatgt 1081 tcccctctga gtgtgcttct atccccaaaa tgccttcaaa ggtgaaaatc aacactggaa 1141 acccaagtat ctgggaaggg caagagtgtg taagtgcaag ttagcctaag gaataggaag 1201 aggttggtaa acagggtagg atcgtgggag ggagtttcgt tactacaggt ccggaccctc 1261 aggacaagaa ccccaccccc actccccaaa ttgcgcatcc cccgccccca tcagaggggg 1321 aggggaagag gttgcggcgc ggcgcatgcg cactgtcgga ttcagcaccg cggtcagagc 1381 cttcgcctcc gctgccggcg cgcaccacca cctccccagc accaaaggct gactgacgtc 1441 actcactagc cctccccaaa ctccccttcc tcgccgcctt ggtcgcgtcc atgctgccgt 1501 gagtccagtc ggaccgcacc acgagaggtg caagataggg aggtgcgggc gcgaccatac 1561 gctctgcggg aattcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 1621 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 1681 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 1741 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac 1801 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 1861 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 1921 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 1981 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 2041 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 2101 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 2161 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag 2221 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 2281 gagctgtaca agtaaagatc tacgcgtggt acctctagag tcgacccggg cggcctcgag 2341 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 2401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 2461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 2521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 2581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 2641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 2701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt 2761 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 2821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 2881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 2941 catcgatacc gtcgactcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 3001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 3061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 3121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 3181 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg actagagcat 3241 ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg 3301 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 3361 gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc agagcttttt gcaaaagcct 3421 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg 3481 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc 3541 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga 3601 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct 3661 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca 3721 caccctaact gacacacatt ccacagctgc attaatgaat cggccaacgc gcggggagag 3781 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3841 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3901 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3961 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4021 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4081 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4141 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4201 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4261 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4321 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4381 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 4441 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4501 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4561 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4621 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4681 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4741 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4801 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4861 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4921 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4981 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5041 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5101 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5161 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5221 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5281 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5341 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5401 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 5461 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 5521 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 5581 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 5641 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 5701 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 5761 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 5821 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 5881 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 5941 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc 6001 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc 6061 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 6121 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 6181 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg 6241 gccacgatgc gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg 6301 accatttccg ggtgcgggac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg 6361 actctgacgg cagtttacga gagagatgat agggt //