LOCUS       Exported                8935 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral overexpression of MYC in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    pmiRA1-MYC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen
            C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng
            X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC, 
            Yang J, Qian Z, Wei M, He C, Chen J
  TITLE     METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and 
            Promotes Leukemogenesis via mRNA m(6)A Modification.
  JOURNAL   Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi: 
            10.1016/j.stem.2017.11.016. Epub 2017 Dec 28.
  PUBMED    29290617
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..238
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     189..209
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     235..259
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             317..343
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             347..373
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             383..1702
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     promoter        1744..1955
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1968..2236
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2265..2274
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2295..2957
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    3036..3624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3089..3109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3507..3518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3698..3931
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4003..4124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4040..4059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4094..4113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4164..4299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4226..4245
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4332..4348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4332..4348)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4345..4367)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4356..4372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4380..4410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4425..4446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4563..4580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4734..5322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4814..4833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5493..6353)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6116..6135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6354..6458)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6526..6544
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6582..6604)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6704..6723
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6917..6939
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6931..6948
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6932..6948
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6963..7189
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7190..7370
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7417..7542
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8035..8268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8453..8497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8764..8880
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
       61 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      121 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      181 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg
      241 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      301 attctagacc accatgtacc cttacgacgt gcccgactac gccgggtacc cttacgacgt
      361 gcccgactac gccggatcca ccatgcccct caacgttagc ttcaccaaca ggaactatga
      421 cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga acttctacca
      481 gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct ggaagaaatt
      541 cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct gctcgccctc
      601 ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg gcgggagctt
      661 ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca tggtgaacca
      721 gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca tccaggactg
      781 tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg cctcctacca
      841 ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg tctgctccac
      901 ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg acccctcggt
      961 ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct cgcaagactc
     1021 cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct ccccgcaggg
     1081 cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca gcgactctga
     1141 ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga ggcaggctcc
     1201 tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac ctcctcacag
     1261 cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg cagcgcctcc
     1321 ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg tcagagtcct
     1381 gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca ccgaggagaa
     1441 tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag
     1501 cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg cccccaaggt
     1561 agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg agcaaaagct
     1621 catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca aacttgaaca
     1681 gctacggaac tcttgtgcgt aagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc
     1741 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     1801 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     1861 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     1921 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     1981 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     2041 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     2101 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     2161 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     2221 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg
     2281 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg
     2341 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca
     2401 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg
     2461 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg
     2521 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc
     2581 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg
     2641 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg
     2701 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca
     2761 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca
     2821 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg
     2881 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga
     2941 cccccattgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg
     3001 ccggacccgg ctccaccgga tctcgctaag tcgacaatca acctctggat tacaaaattt
     3061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3541 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3601 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     3661 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     3721 cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     3781 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     3841 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     3901 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     3961 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     4021 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     4141 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4201 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4261 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagaccaaa
     4321 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4381 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4441 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4501 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4561 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4621 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4681 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4741 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4801 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4861 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4921 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4981 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5041 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5101 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5161 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5221 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5281 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5341 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5401 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5461 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5521 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5581 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5641 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5701 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5761 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5821 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5881 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5941 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6001 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6061 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6121 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6181 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6241 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6301 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6361 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6421 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6481 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     6541 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     6601 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     6661 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     6721 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6781 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     6841 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     6901 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgacg
     6961 cgtgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7021 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7081 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7141 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7201 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7261 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7321 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7381 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7441 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7501 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7561 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7621 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7681 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7741 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7801 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7861 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga
     7921 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7981 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8041 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8101 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8161 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8221 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8281 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8341 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8401 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8461 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8521 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8581 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8641 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8701 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     8761 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8821 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     8881 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggca
//
