LOCUS Exported 6892 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pGFAP::MyrTomato SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6892) AUTHORS Nam HS, Benezra R TITLE High levels of Id1 expression define B1 type adult neural stem cells. JOURNAL Cell Stem Cell. 2009 Nov 6. 5(5):515-26. PUBMED 19896442 REFERENCE 2 (bases 1 to 6892) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6892 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 115..136 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 151..181 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 189..205 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 194..216 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 213..229 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 213..229 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2506..3936 /codon_start=1 /product="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" /label=tdTomato /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE RSEGRHHLFLYGMDELYK" primer_bind complement(4505..4522) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4505..4521) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4514..4536) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(4730..4749) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4849..4871 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4909..4927) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4995..5099 /gene="bla" /label=AmpR promoter CDS 5100..5960 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5318..5337) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6131..6719 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6620..6639 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6873..6890 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa 241 ttcgagctcg gtaccagatc tgagctccca cctccctctc tgtgctggga ctcacagagg 301 gagacctcag gaggcagtct gtccatcaca tgtccaaatg cagagcatac cctgggctgg 361 gcgcagtggc gcacaactgt aattccagca ctttgggagg ctgatgtgga aggatcactt 421 gagcccagaa gttctagacc agcctgggca acatggcaag accctatctc tacaaaaaaa 481 gttaaaaaat cagccacgtg tggtgacaca cacctgtagt cccagctatt caggaggctg 541 aggtgagggg atcacttaag gctgggaggt tgaggctgca gtgagtcgtg gttgcgccac 601 tgcactccag cctgggcaac agtgagaccc tgtctcaaaa gacaaaaaaa aaaaaaaaaa 661 aaaaaagaac atatcctggt gtggagtagg ggacgctgct ctgacagagg ctcgggggcc 721 tgagctggct ctgtgagctg gggaggaggc agacagccag gccttgtctg caagcagacc 781 tggcagcatt gggctggccg ccccccaggg cctcctcttc atgcccagtg aatgactcac 841 cttggcacag acacaatgtt cggggtgggc acagtgcctg cttcccgccg caccccagcc 901 cccctcaaat gccttccgag aagcccattg agcagggggc ttgcattgca ccccagcctg 961 acagcctggc atcttgggat aaaagcagca cagcccccta ggggctgccc ttgctgtgtg 1021 gcgccaccgg cggtggagaa caaggctcta ttcagcctgt gcccaggaaa ggggatcagg 1081 ggatgcccag gcatggacag tgggtggcag ggggggagag gagggctgtc tgcttcccag 1141 aagtccaagg acacaaatgg gtgaggggac tgggcagggt tctgaccctg tgggaccaga 1201 gtggagggcg tagatggacc tgaagtctcc agggacaaca gggcccaggt ctcaggctcc 1261 tagttgggcc cagtggctcc agcgtttcca aacccatcca tccccagagg ttcttcccat 1321 ctctccaggc tgatgtgtgg gaactcgagg aaataaatct ccagtgggag acggaggggt 1381 ggccagggaa acggggcgct gcaggaataa agacgagcca gcacagccag ctcatgtgta 1441 acggctttgt ggagctgtca aggcctggtc tctgggagag aggcacaggg aggccagaca 1501 aggaaggggt gacctggagg gacagatcca ggggctaaag tcctgataag gcaagagagt 1561 gccggccccc tcttgcccta tcaggacctc cactgccaca tagaggccat gattgaccct 1621 tagacaaagg gctggtgtcc aatcccagcc cccagcccca gaactccagg gaatgaatgg 1681 gcagagagca ggaatgtggg acatctgtgt tcaagggaag gactccagga gtctgctggg 1741 aatgaggcct agtaggaaat gaggtggccc ttgagggtac agaacaggtt cattcttcgc 1801 caaattccca gcaccttgca ggcacttaca gctgagtgag ataatgcctg ggttatgaaa 1861 tcaaaaagtt ggaaagcagg tcagaggtca tctggtacag cccttccttc cctttttttt 1921 tttttttttt gtgagacaag gtctctctct gttgcccagg ctggagtggc gcaaacacag 1981 ctcactgcag cctcaaccta ctgggctcaa gcaatcctcc agcctcagcc tcccaaagtg 2041 ctgggattac aagcatgagc caccccactc agccctttcc ttccttttta attgatgcat 2101 aataattgta agtattcatc atggtccaac caaccctttc ttgacccacc ttcctagaga 2161 gagggtcctc ttgcttcagc ggtcagggcc ccagacccat ggtctggctc caggtaccac 2221 ctgcctcatg caggagttgg cgtgcccagg aagctctgcc tctgggcaca gtgacctcag 2281 tggggtgagg ggagctctcc ccatagctgg gctgcggccc aaccccaccc cctcaggcta 2341 tgccaggggg tgttgccagg ggcacccggg catcgccagt ctagcccact ccttcataaa 2401 gccctcgcat cccaggagcg agcagagcca gagcaggttg gagaggagac gcatcacctc 2461 cgctgctcgc ggggatccac catgggttgc tgtttctcca agaccatggt gagcaaggga 2521 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc 2581 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 2641 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag 2701 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag 2761 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg 2821 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg 2881 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag 2941 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc 3001 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag 3061 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac 3121 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc 3181 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac 3241 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg 3301 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 3361 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc 3421 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac 3481 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 3541 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg 3601 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc 3661 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac 3721 caggccctga agctgaagga cggcggccgc tacctggtgg agttcaagac catctacatg 3781 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc 3841 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac 3901 ctgttcctgt acggcatgga cgagctgtac aagtaaggat ccgcagatcc cggccagata 3961 ccgatgctgc cgcagcaaaa gcaggagcag atgccgccgt cgcaggcgaa gatgtcgcag 4021 acggaggagg cgatgctgcc ggcggaggag gcgaagtaag tagagggctg ggctgggctg 4081 tggggggtgt ggggtgcggg actgggcagt ctgggagtcc ctctcaccac ttttcttacc 4141 tttctaggat gctgccgtcg ccgccgctca tacaccataa ggtgtaaaaa atactagatg 4201 cacagaatag caagtccatc aaaactcctg cgtgagaatt ttaccagact tcaagagcat 4261 ctcgccacat cttgaaaaat gccaccgtcc gatgaaaaac aggagcctgc taaggaacaa 4321 tgccacctgt caataaatgt tgaaaactca tcccattcct gcctcttggt ccttgggctt 4381 ggggaggggt gcgcggatgt ggttagggaa catgactggt caaatgggaa gggcttcaaa 4441 agaattccca atattgacta ccaagccacc tgtacagatc tgcctgcagg catgcaagct 4501 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 4561 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 4621 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc 4681 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct 4741 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 4801 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 4861 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 4921 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 4981 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 5041 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 5101 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 5161 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 5221 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 5281 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 5341 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 5401 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 5461 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 5521 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 5581 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 5641 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 5701 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 5761 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 5821 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 5881 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 5941 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 6001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 6061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 6121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 6181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 6241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag 6301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 6361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 6421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 6481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 6541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 6601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 6661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 6721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 6781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 6841 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag ga //