LOCUS       Exported                9769 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    pD649-HAsp-ROBO1-COMP5AP-AviTag-9xHis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5419..5442
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7102..7146
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7147..7173
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7313..7330)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7319..7430
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7548..7568)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7553..7882
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7733..7868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7795..7814
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7930..7977
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7996..8595
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7996..8015)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8492..8512
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8725..8846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8762..8781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8816..8835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8899..9759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9522..9541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9760..82)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca acttattccc
     2761 gatcctgagg atgtggaacg gggcaacgat cacggcaccc ctattcctac cagcgacaac
     2821 gacgacaaca gcctgggcta cacaggcagc agactgcggc aagaggactt cccacctcgg
     2881 atcgtggaac accccagcga tctgatcgtg tccaaaggcg aacccgccac actgaactgc
     2941 aaggccgaag gcagacccac acctaccatc gagtggtaca aaggcggcga gagagtggaa
     3001 accgacaagg acgatcctcg gagccacaga atgctgctgc ctagcggcag cctgttcttc
     3061 ctgagaatcg tgcacggcag aaagagcaga cctgacgagg gcgtgtacgt gtgcgtggcc
     3121 agaaattatc tgggcgaagc cgtgtctcac aacgccagcc tggaagtggc catcctgagg
     3181 gacgacttca gacagaaccc ctccgatgtg atggtggccg tgggagaacc tgccgtgatg
     3241 gaatgccaac ctcctagagg acaccccgag cctaccatca gctggaagaa ggatggcagc
     3301 cctctggacg acaaggatga gcggatcaca atcagaggcg gcaagctgat gatcacctac
     3361 accagaaagt ccgacgccgg caaatacgtg tgtgtgggca ccaacatggt cggagagcgg
     3421 gaatctgaag tggccgagct gaccgtgctg gaaagaccca gcttcgtgaa gaggcccagc
     3481 aatctggccg tgaccgtgga tgatagcgcc gagtttaagt gcgaggccag aggcgatcct
     3541 gtgcctacag ttagatggcg gaaggacgat ggcgagctgc ccaagagcag atacgagatc
     3601 agggacgatc acaccctgaa gatccggaaa gtgacagccg gcgacatggg cagctatacc
     3661 tgtgtggccg agaatatggt cggaaaggcc gaggccagcg ccactctgac agttcaagag
     3721 cctcctcact tcgtggtcaa gcccagagat caggtggtgg ctctgggcag aaccgtgacc
     3781 tttcagtgtg aagccaccgg caatccccag cctgccatct tttggagaag agagggcagc
     3841 cagaacctgc tgttcagcta ccagcctcca cagagcagca gcagattcag cgttagccag
     3901 accggcgacc tgaccatcac caacgtgcag agatccgacg tgggctacta catctgtcag
     3961 accctgaatg tggccggcag catcatcacc aaggcctatc tggaagtgac cgacgtgatc
     4021 gccgacagac ctcctccagt gatcagacag ggccccgtga atcagaccgt ggctgtggat
     4081 ggcaccttcg tgctgtcttg tgtggccacc ggatctcccg tgcctacaat cctgtggcgg
     4141 aaagatggcg tgctggtgtc tacccaggac agcagaatca agcagctgga aaacggcgtg
     4201 ctgcagatca gatacgccaa gctgggcgac accggcagat acacctgtat cgcctctaca
     4261 cctagcggcg aggctacttg gagcgcctac attgaggtgc aagagttcgg cgtgccagtg
     4321 cagcctccta gacctacaga ccccaatctg atccccagcg ctcccagcaa gcctgaagtg
     4381 actgatgtgt cccggaacac cgtgacactg agctggcagc ccaacctgaa ttctggcgcc
     4441 acaccaacca gctacatcat cgaggccttt agccacgcca gcggcagctc ttggcagaca
     4501 gtggccgaaa acgtgaaaac cgagacaagc gccatcaagg gcctgaagcc taacgctatc
     4561 tacctgttcc tcgtgcgggc tgccaatgcc tacggcatct ctgatcctag ccagatcagc
     4621 gaccctgtga aaacccagga cgtgctgcct acatctcagg gcgtcgacca caaacaggtg
     4681 cagagagaac tgggcaacgc tgtgctgcat ctgcacaacc ctacagtgct gagcagctcc
     4741 agcatcgagg tgcactggac agtggatcag cagagccagt acatccaggg ctacaagatc
     4801 ctgtacagac ccagcggagc caaccacggc gaatctgatt ggctggtgtt cgaagtgcgg
     4861 acccctgcca agaacagcgt ggtcatcccc gatctgagaa agggcgtgaa ctatgagatc
     4921 aaggccaggc cattcttcaa cgagttccag ggcgccgaca gcgagatcaa gttcgccaag
     4981 acactggaag aggctccctc cgctcctcca caaggcgtga cagtgtccaa gaacgacggc
     5041 aatggcaccg ccatcctggt gtcatggcaa cctccacctg aggataccca gaacggcatg
     5101 gtccaagagt acaaagtgtg gtgcctgggg aacgagacac gctaccacat caacaagacc
     5161 gtggacggct ctaccttcag cgtcgtgatc ccttttctgg tgcccggcat ccggtactct
     5221 gtggaagttg ctgcttctac cggcgctggc tctggcgtga aaagcgagcc tcagttcatt
     5281 cagctggacg cccacggcaa ccctgtgtct ccagaggacc aagtgtctct ggcccagcag
     5341 atttccgacg tcgtgaagca acctggcgcg ccggaagatc aagttgatcc aagattgatt
     5401 gatggcaagg gcggatctct ggaggtgctg ttccagggac caggcggaag cggcaatccc
     5461 ggcggcgatc tggcccccca gatgctgcgc gagctgcagg agaccaatgc cgccctgcag
     5521 gacgtgcgcg agctgctgcg ccaccgcgtg aaggagatca ccttcctgaa gaacaccgtg
     5581 atggagtgcg acgcctgcgg tatgcagccc tctagaggct ccggctccat catcccagtt
     5641 gaggaggaga acccggactt ctggaaccgc gaggcagccg aggccctggg tgccgccaag
     5701 aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg cgatgggatg
     5761 ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga caaactgggg
     5821 cctgagatac ccctggccat ggaccgcttc ccatatgtgg ctctgtccaa gacatacaat
     5881 gtagacaaac atgtgccaga cagtggagcc acagccacgg cctacctgtg cggggtcaag
     5941 ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg caacacgaca
     6001 cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcagggaa gtcagtggga
     6061 gtggtaacca ccacacgagt gcagcacgcc tcgccagccg gcacctacgc ccacacggtg
     6121 aaccgcaact ggtactcgga cgccgacgtg cctgcctcgg cccgccagga ggggtgccag
     6181 gacatcgcta cgcagctcat ctccaacatg gacattgacg tgatcctagg tggaggccga
     6241 aagtacatgt ttcgcatggg aaccccagac cctgagtacc cagatgacta cagccaaggt
     6301 gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagcg ccagggtgcc
     6361 cggtatgtgt ggaaccgcac tgagctcatg caggcttccc tggacccgtc tgtgacccat
     6421 ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga ctccacactg
     6481 gacccctccc tgatggagat gacagaggct gccctgcgcc tgctgagcag gaacccccgc
     6541 ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga aagcagggct
     6601 taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc gggccagctc
     6661 accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt cttctccttc
     6721 ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa ggcccgggac
     6781 aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct caaggacggc
     6841 gcccggccgg atgttaccga gagcgagagc gggagccccg agtatcggca gcagtcagca
     6901 gtgcccctgg acgaagagac ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg
     6961 caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt catggccttc
     7021 gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccccgccgg caccaccgac
     7081 gccgcgcacc cgggtggcgg aggactgaac gacatcttcg aggctcagaa aatcgaatgg
     7141 cacgaacatc accatcacca tcaccatcac cattaaggcc ggcctgtagt cactggcaca
     7201 acgcgttacc cggtaagcca atcgggtata cacggtcgtc atactgcaga cagggttctt
     7261 ctactttgca agatagtctt gagtagtaaa ataaatagat agagaaaatc agcctcgact
     7321 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7381 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc acaacactca
     7441 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7501 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     7561 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7621 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7681 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7741 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7801 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     7861 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     7921 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7981 gtgaggaact aaaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8041 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8101 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8161 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8221 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8281 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8341 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     8401 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     8461 gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga gcggctcggc
     8521 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     8581 aagcccggtg cctgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8641 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8701 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8821 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8881 ttggcgtaat catggtcatt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8941 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9001 gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc
     9061 ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9121 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9181 agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc
     9241 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9301 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9361 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9421 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9481 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9541 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9601 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9661 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9721 aaagggaata agggcgacac ggaaatgttg aatactcata ttcttcctt
//
