LOCUS       Exported               10255 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    pD649-HAsp-JAG1-COMP5AP-AviTag-9xHis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10255)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10255
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5905..5928
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7588..7632
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7633..7659
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7799..7816)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7805..7916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8034..8054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8039..8368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8219..8354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8281..8300
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8416..8463
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8482..9081
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8482..8501)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8978..8998
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9211..9332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9248..9267)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9302..9321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(9385..10245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10008..10027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10246..82)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca atttgagctg
     2761 gaaatcctga gcatgcagaa cgtgaacggc gagctgcaga atggcaattg ctgtggcgga
     2821 gccagaaatc ccggcgacag aaagtgcacc agagatgagt gcgacaccta cttcaaagtg
     2881 tgcctgaaag aataccagag ccgcgtgaca gccggcggac cttgttcttt tggcagcggc
     2941 tctacacctg tgatcggcgg caacaccttc aacctgaagg ccagcagagg caacgaccgg
     3001 aacagaatcg tgctgccctt cagcttcgcc tggcctagaa gctacaccct gctggtggaa
     3061 gcctgggaca gcagcaatga taccgtgcag cccgacagca tcatcgagaa ggcctctcac
     3121 agcggcatga tcaaccccag cagacagtgg cagaccctga agcagaatac cggcgtggcc
     3181 cacttcgagt accagatcag agtgacctgc gacgactact actacggctt cggctgcaac
     3241 aagttctgca gaccccggga cgatttcttc ggccactatg cctgcgacca gaacggcaac
     3301 aagacctgca tggaaggctg gatgggcccc gagtgcaata gagccatctg cagacagggc
     3361 tgtagcccca agcacggcag ctgtaaactg cccggcgatt gcagatgcca gtatggctgg
     3421 cagggcctgt actgcgacaa gtgcattcct catcctggct gcgtgcacgg aatctgcaat
     3481 gagccttggc agtgcctgtg cgagacaaac tggggaggcc agctgtgcga caaggacctg
     3541 aattactgcg gcacccacca gccttgcctg aatggcggca catgtagcaa cacaggcccc
     3601 gacaagtacc agtgctcttg ccctgagggc tactctggcc ccaattgcga gattgccgaa
     3661 cacgcctgtc tgagcgaccc ctgccacaat agaggcagct gcaaagagac aagcctgggc
     3721 ttcgagtgcg agtgttctcc tggatggaca ggccctacct gcagcaccaa catcgacgac
     3781 tgcagcccta acaactgcag ccacggcgga acctgtcagg acctggtcaa cggctttaag
     3841 tgcgtgtgcc ctcctcagtg gaccggcaaa acatgccagc tggacgccaa cgagtgcgaa
     3901 gccaagcctt gtgtgaacgc caagagctgc aagaacctga tcgccagcta ctactgcgac
     3961 tgcctgccag gatggatggg acagaactgc gacatcaaca tcaacgattg cctgggccag
     4021 tgccagaacg acgccagctg tagagatctg gtcaatggct accggtgcat ctgcccacct
     4081 ggatatgcag gcgatcactg cgagcgggac attgacgagt gtgccagcaa tccctgtctg
     4141 aacggcggcc actgtcagaa cgagatcaac cggttccagt gtctgtgccc caccggcttt
     4201 agcggcaatc tgtgtcagct ggacatcgac tattgcgagc ccaatccatg ccagaatggc
     4261 gcccagtgct acaacagagc cagcgactac ttctgcaagt gccccgagga ttacgagggc
     4321 aagaactgct cccacctgaa ggaccactgc cggaccacac cttgcgaagt gatcgatagc
     4381 tgcaccgtgg ctatggccag caacgataca ccagaaggcg tgcggtacat cagcagcaac
     4441 gtgtgtggac ctcacggcaa gtgcaagtct cagagcggcg gcaagttcac ctgtgactgt
     4501 aacaagggct tcaccggcac ctactgccac gagaacatca atgactgcga gagcaacccc
     4561 tgcagaaacg gcggcacttg tatcgacggc gtgaacagct acaagtgtat ctgcagcgac
     4621 ggatgggagg gcgcctactg cgagactaat atcaacgact gctctcagaa cccttgccac
     4681 aacggcggga cctgcagaga tctcgtgaac gacttctact gcgattgcaa gaacggctgg
     4741 aagggcaaga cctgtcacag cagagatagc cagtgcgacg aggccacctg taacaatggc
     4801 ggaacttgct acgatgaggg cgacgccttc aagtgcatgt gtcctggtgg atgggaaggc
     4861 accacatgca atatcgcccg gaacagcagc tgtctgccta atccatgtca taacggtggc
     4921 acctgtgtcg tgaatggcga gagctttacc tgcgtgtgca aagaaggctg ggagggacct
     4981 atctgcgccc agaacaccaa cgactgtagc ccacatcctt gctacaactc cggcacatgc
     5041 gtggacggcg acaactggta cagatgcgaa tgcgcccctg gcttcgccgg acctgattgc
     5101 cggatcaata tcaatgagtg tcagagcagc ccttgcgcct tcggcgctac ttgtgtggat
     5161 gagatcaacg gctaccgctg cgtgtgtcca ccaggccata gcggagccaa gtgtcaagaa
     5221 gtgtccggca gaccttgcat caccatgggc agcgttatcc ccgatggcgc caagtgggac
     5281 gacgattgca atacctgcca gtgcctcaac ggcagaatcg cctgctccaa agtttggtgc
     5341 ggccccagac cttgtctgct gcacaaggga cacagcgagt gtcctagcgg ccagagctgt
     5401 atccccatcc tggacgatca gtgcttcgtg cacccttgta caggcgtggg cgagtgtaga
     5461 agcagctctc tgcagcccgt gaaaacaaag tgtaccagcg atagctacta ccaggacaac
     5521 tgcgccaaca tcaccttcac gttcaacaaa gaaatgatga gccctggcct gaccaccgag
     5581 cacatctgta gcgagctgag aaacctgaac atcctgaaga acgtgtccgc cgagtacagc
     5641 atctatatcg cctgcgagcc ttctcctagc gccaacaacg aaatccacgt ggccatcagc
     5701 gccgaggaca tcagagatga cggcaacccc atcaaagaga tcaccgacaa gatcatcgac
     5761 ctggtgtcca agcgcgacgg caactcttct ctgattgctg ccgtcgctga agtgcgggtc
     5821 cagagaaggc ccctgaagaa tagaaccgat ggcgcgccgg aagatcaagt tgatccaaga
     5881 ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg cggaagcggc
     5941 aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac caatgccgcc
     6001 ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt cctgaagaac
     6061 accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg ctccatcatc
     6121 ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc
     6181 gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat
     6241 gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa gaaggacaaa
     6301 ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct gtccaagaca
     6361 tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta cctgtgcggg
     6421 gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa ccagtgcaac
     6481 acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc agggaagtca
     6541 gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac ctacgcccac
     6601 acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg
     6661 tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat cctaggtgga
     6721 ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga tgactacagc
     6781 caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc gaagcgccag
     6841 ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga cccgtctgtg
     6901 acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca ccgagactcc
     6961 acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct gagcaggaac
     7021 ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca tcatgaaagc
     7081 agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga gagggcgggc
     7141 cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc
     7201 tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc tggcaaggcc
     7261 cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta tgtgctcaag
     7321 gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta tcggcagcag
     7381 tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt gttcgcgcgc
     7441 ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc gcacgtcatg
     7501 gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc cgccggcacc
     7561 accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc tcagaaaatc
     7621 gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc tgtagtcact
     7681 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     7741 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     7801 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7861 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     7921 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     8041 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8101 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8161 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8281 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8341 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8401 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     8461 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     8521 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     8581 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8641 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8701 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8761 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8821 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8881 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8941 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     9001 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     9061 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     9121 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9181 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     9241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9361 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
     9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9481 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
     9541 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9661 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
     9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9961 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
