LOCUS Exported 13789 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION express CARPID BASU-dCasRx fusion protein in mammalian cells. ACCESSION . VERSION . KEYWORDS CARPID BASU-dCasRx SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13789) AUTHORS Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye J, Chen F, Taipale J, Chan KM, Zhang L, Yan J TITLE CRISPR-assisted detection of RNA-protein interactions in living cells. JOURNAL Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 10.1038/s41592-020-0866-0. PUBMED 32572232 REFERENCE 2 (bases 1 to 13789) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13789 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..181 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 228..353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 846..1079 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1264..1308 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1606..1723 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 1848..3026 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2079..3017 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 2974..2994 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3843..3863 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 3873..6770 /codon_start=1 /product="RNA-targeting Type VI CRISPR protein from Ruminococcus flavefaciens strain XPD3002 (Konermann et al., 2018)" /label=RfxCas13d /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS" CDS 6780..6800 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6810..6836 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 6852..6905 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6906..7622 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 6906..7619 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(6948..6969) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7209..7228) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7556..7577 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7647..8235 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7700..7720) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8118..8129) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(8238..8254) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8239..8255) /label=pBluescriptKS /note="For pBluescript vector" LTR 8760..8940 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8966..8983) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8972..9196 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9242..9670 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9329..9348) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9539..9560 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9679..9699) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9684..10013 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9864..9999 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9926..9945 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 10061..10108 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 10127..10501 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 10631..10752 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10668..10687) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10722..10741 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10801..10817) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10801..10817) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10814..10836) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10825..10841 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10849..10879) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10894..10915 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(11032..11049) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11203..11791) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11283..11302) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11962..12822) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12585..12604 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12823..12927) /gene="bla" /label=AmpR promoter primer_bind complement(13002..13021) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 13193..13572 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 13574..13772 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 13727..13747 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" ORIGIN 1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 1741 cagcagagat ccagtttggt taattaatgc aaagatggat aaagttttaa acagagagga 1801 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc 1861 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 1921 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 1981 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 2041 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc 2101 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc 2161 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc 2221 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg 2281 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc 2341 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg 2401 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg 2461 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga 2521 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc 2581 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg 2641 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg 2701 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg 2761 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct 2821 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca 2881 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat 2941 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt 3001 tttttcttcc atttcaggtg tcgtgacgta cggccaccat gagcggcaag ttgtctgaat 3061 cagagattag gtttggactg aaaacggagg tgatgggcca acatcttatc tatcacgatg 3121 ttttgtccag cacacaaaag acagcccatg aattggcaaa taacaatgcc cccgagggta 3181 ccctcgtggt tgcagataaa cagacagccg gtaggggcgg gatgagccga gtgtggcatt 3241 ctcaagaagg aaacggagta tggatgagtt tgatattgcg cccggacatt ccgctccaaa 3301 agacgcccca acttaccttg ctggcagctg tagcggtggt tcagggaata gaagaggccg 3361 cgggcataca gactgatata aagtggccga acgacatact cattaacggg aaaaaaacag 3421 tagggatact taccgaaatg caagctgagg aggatcgagt gcgctccgtg attattggta 3481 ttggcattaa tgtaaatcag caacccaatg atttccccga tgagctcaag gacattgcga 3541 cgagtctgtc ccaggcagct ggggaaaaga tcgacagggc aggtgtaatc cagcatatct 3601 tgctttgttt cgaaaagcga taccgagact acatgaccca cggtttcacg cccatcaagc 3661 tcttgtggga gtcatacgcc ctcggtattg gcacgaacat gagggcgaga actctcaacg 3721 ggacatttta cggtaaggcg ttgggtattg acgacgaagg ggtacttttg ttggaaacta 3781 atgaaggcat taagaagata tacagcgcgg acatcagcct taggcgtacg gccaccatga 3841 gccccaagaa gaagagaaag gtggaggcca gcatcgaaaa aaaaaagtcc ttcgccaagg 3901 gcatgggcgt gaagtccaca ctcgtgtccg gctccaaagt gtacatgaca accttcgccg 3961 aaggcagcga cgccaggctg gaaaagatcg tggagggcga cagcatcagg agcgtgaatg 4021 agggcgaggc cttcagcgct gaaatggccg ataaaaacgc cggctataag atcggcaacg 4081 ccaaattcag ccatcctaag ggctacgccg tggtggctaa caaccctctg tatacaggac 4141 ccgtccagca ggatatgctc ggcctgaagg aaactctgga aaagaggtac ttcggcgaga 4201 gcgctgatgg caatgacaat atttgtatcc aggtgatcca taacatcctg gacattgaaa 4261 aaatcctcgc cgaatacatt accaacgccg cctacgccgt caacaatatc tccggcctgg 4321 ataaggacat tattggattc ggcaagttct ccacagtgta tacctacgac gaattcaaag 4381 accccgagca ccatagggcc gctttcaaca ataacgataa gctcatcaac gccatcaagg 4441 cccagtatga cgagttcgac aacttcctcg ataaccccag actcggctat ttcggccagg 4501 cctttttcag caaggagggc agaaattaca tcatcaatta cggcaacgaa tgctatgaca 4561 ttctggccct cctgagcgga ctggcgcact gggtggtcgc taacaacgaa gaagagtcca 4621 ggatctccag gacctggctc tacaacctcg ataagaacct cgacaacgaa tacatctcca 4681 ccctcaacta cctctacgac aggatcacca atgagctgac caactccttc tccaagaact 4741 ccgccgccaa cgtgaactat attgccgaaa ctctgggaat caaccctgcc gaattcgccg 4801 aacaatattt cagattcagc attatgaaag agcagaaaaa cctcggattc aatatcacca 4861 agctcaggga agtgatgctg gacaggaagg atatgtccga gatcaggaaa aatcataagg 4921 tgttcgactc catcaggacc aaggtctaca ccatgatgga ctttgtgatt tataggtatt 4981 acatcgaaga ggatgccaag gtggctgccg ccaataagtc cctccccgat aatgagaagt 5041 ccctgagcga gaaggatatc tttgtgatta acctgagggg ctccttcaac gacgaccaga 5101 aggatgccct ctactacgat gaagctaata gaatttggag aaagctcgaa aatatcatgc 5161 acaacatcaa ggaatttagg ggaaacaaga caagagagta taagaagaag gacgccccta 5221 gactgcccag aatcctgccc gctggccgtg atgtttccgc cttcagcaaa ctcatgtatg 5281 ccctgaccat gttcctggat ggcaaggaga tcaacgacct cctgaccacc ctgattaata 5341 aattcgataa catccagagc ttcctgaagg tgatgcctct catcggagtc aacgctaagt 5401 tcgtggagga atacgccttt ttcaaagact ccgccaagat cgccgatgag ctgaggctga 5461 tcaagtcctt cgctagaatg ggagaaccta ttgccgatgc caggagggcc atgtatatcg 5521 acgccatccg tattttagga accaacctgt cctatgatga gctcaaggcc ctcgccgaca 5581 ccttttccct ggacgagaac ggaaacaagc tcaagaaagg caagcacggc atgagaaatt 5641 tcattattaa taacgtgatc agcaataaaa ggttccacta cctgatcaga tacggtgatc 5701 ctgcccacct ccatgagatc gccaaaaacg aggccgtggt gaagttcgtg ctcggcagga 5761 tcgctgacat ccagaaaaaa cagggccaga acggcaagaa ccagatcgac aggtactacg 5821 aaacttgtat cggaaaggat aagggcaaga gcgtgagcga aaaggtggac gctctcacaa 5881 agatcatcac cggaatgaac tacgaccaat tcgacaagaa aaggagcgtc attgaggaca 5941 ccggcaggga aaacgccgag agggagaagt ttaaaaagat catcagcctg tacctcaccg 6001 tgatctacca catcctcaag aatattgtca atatcaacgc caggtacgtc atcggattcc 6061 attgcgtcga gcgtgatgct caactgtaca aggagaaagg ctacgacatc aatctcaaga 6121 aactggaaga gaagggattc agctccgtca ccaagctctg cgctggcatt gatgaaactg 6181 cccccgataa gagaaaggac gtggaaaagg agatggctga aagagccaag gagagcattg 6241 acagcctcga gagcgccaac cccaagctgt atgccaatta catcaaatac agcgacgaga 6301 agaaagccga ggagttcacc aggcagatta acagggagaa ggccaaaacc gccctgaacg 6361 cctacctgag gaacaccaag tggaatgtga tcatcaggga ggacctcctg agaattgaca 6421 acaagacatg taccctgttc gcaaacaagg ccgtcgccct ggaagtggcc aggtatgtcc 6481 acgcctatat caacgacatt gccgaggtca attcctactt ccaactgtac cattacatca 6541 tgcagagaat tatcatgaat gagaggtacg agaaaagcag cggaaaggtg tccgagtact 6601 tcgacgctgt gaatgacgag aagaagtaca acgataggct cctgaaactg ctgtgtgtgc 6661 ctttcggcta ctgtatcccc aggtttaaga acctgagcat cgaggccctg ttcgatagga 6721 acgaggccgc caagttcgac aaggagaaaa agaaggtgtc cggcaattcc ggatccggac 6781 ctaagaaaaa gaggaaggtg gcggccgctt acccatacga tgttccagat tacgctgcta 6841 gcggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg 6901 gcccagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 6961 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 7021 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 7081 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 7141 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 7201 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 7261 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 7321 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 7381 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 7441 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 7501 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 7561 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 7621 aagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 7681 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 7741 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 7801 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 7861 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 7921 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 7981 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 8041 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 8101 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 8161 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 8221 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca 8281 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 8341 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 8401 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 8461 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt 8521 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca 8581 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct 8641 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga 8701 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg 8761 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 8821 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 8881 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 8941 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt 9001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 9061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 9121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 9181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc 9241 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 9301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 9361 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 9421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 9481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 9541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 9601 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 9661 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 9721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 9781 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 9841 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 9901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 9961 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 10021 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 10081 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 10141 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 10201 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 10261 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 10321 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 10381 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 10441 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 10501 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat 10561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt 10621 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 10681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 10741 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg 10801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 10861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 10921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 10981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 11041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 11101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 11161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 11221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 11281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 11341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 11401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 11461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 11521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 11581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 11641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 11701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 11761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 11821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 11881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 11941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 12001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 12061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 12121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 12181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 12241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 12301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 12361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 12421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 12481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 12541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 12601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 12661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 12721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 12781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 12841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 12901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga 12961 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc 13021 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg 13081 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt 13141 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga 13201 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 13261 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 13321 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 13381 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 13441 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 13501 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 13561 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 13621 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 13681 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 13741 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtact //