LOCUS Exported 4226 bp ds-DNA linear SYN 20-JUN-2021 DEFINITION Expression of sfGFP-TetR-p300HAT fusion protein. ACCESSION . VERSION . KEYWORDS pETduet+sfGFP-TetR-p300HAT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4226) AUTHORS Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK TITLE Organization of Chromatin by Intrinsic and Regulated Phase Separation. JOURNAL Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19. PUBMED 31543265 REFERENCE 2 (bases 1 to 4226) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4226 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 9..35 /codon_start=1 /product="9xHis affinity tag" /label=9xHis /translation="HHHHHHHHH" CDS 36..56 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 60..773 /codon_start=1 /product="Aequoria victoria green fluorescent protein" /label=GFP /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" primer_bind complement(90..118) /label=GFP-R /note="GFP, reverse primer. Does NOT anneal to EGFP" primer_bind 713..733 /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" regulatory 2376..2385 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind 2693..2712 /label=T7 /note="T7 promoter, forward primer" promoter 2693..2711 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 2712..2736 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 ccatgggaca tcaccatcat caccaccatc atcacgaaaa cctgtatttt cagggatcca 61 tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg 121 atgttaatgg gcacaaattt tctgtccgtg gagagggtga aggtgatgca acaaacggaa 181 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg 241 tcactacttt aacttatggt gttcaatgct tttcaagata cccagatcat atgaaacagc 301 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatctttca 361 aagatgacgg gacctacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta 421 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat 481 tggaatacaa ctttaactca cacaatgtat acatcacggc agacaaacaa aagaatggaa 541 tcaaagctaa cttcaaaatt agacacaacg ttgaagatgg aagcgttcaa ctagcagacc 601 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc 661 tgtccacaca atctgtcctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc 721 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaaggcgggt 781 ccggtggaag tggcgggtcc gcacggctga acagagaatc ggttattgat gcggcactgg 841 aactgctgaa tgagacaggg attgacgggc tgacgacccg caagctggcg cagaagctgg 901 gaatagaaca gccgacactt tactggcatg tgaaaaataa acgggcgtta ctggatgcgc 961 tggcggtgga gatcttggcg cgtcatcatg attattcact gcctgcggcg ggggaatctt 1021 ggcagtcatt tctgcgcaat aatgcaatga gtttccgccg ggcgctgctg cgttaccgtg 1081 acggggcaaa agtgcacctc ggcacccgcc ctgatgaaaa acagtatgat acggtggaaa 1141 cccagttacg ctttatgaca gaaaacggct tttcactgcg cgacgggtta tatgcgattt 1201 cagcggtcag tcattttacc cttggtgccg tactggagca gcaggagcat actgccgccc 1261 tgaccgaccg ccctgcagca ccggacgaaa acctgccgcc gctattgcgg gaagcgctgc 1321 agattatgga cagtgatgat ggtgagcagg cctttctgca tggcctggag agcctgatcc 1381 gggggtttga ggtgcagctt acggcactgt tgcaaatagt cggtggtgat aaacttatca 1441 tccccttttg cggaggttcg ggagggagcg gtggatctaa gttttctgct aaaaggttgc 1501 catctaccag acttggcacc tttctagaga atcgtgtgaa tgactttctg aggcgacaga 1561 atcaccctga gtcaggagag gtcactgtta gagtagttca tgcttctgac aaaaccgtgg 1621 aagtaaaacc aggcatgaaa gcaaggtttg tggacagtgg agagatggca gaatcctttc 1681 cataccgaac caaagccctc tttgcctttg aagaaattga tggtgttgac ctgtgcttct 1741 ttggcatgca tgttcaagag tatggctctg actgccctcc acccaaccag aggagagtat 1801 acatatctta cctcgatagt gttcatttct tccgtcctaa atgcttgagg actgcagtct 1861 atcatgaaat cctaattgga tatttagaat atgtcaagaa attaggttac acaacagggc 1921 atatttgggc atgtccacca agtgagggag atgattatat cttccattgc catcctcctg 1981 accagaagat acccaagccc aagcgactgc aggaatggta caaaaaaatg cttgacaagg 2041 ctgtatcaga gcgtattgtc catgactaca aggatatttt taaacaagct actgaagata 2101 gattaacaag tgcaaaggaa ttgccttatt tcgagggtga tttctggccc aatgttctgg 2161 aagaaagcat taaggaactg gaacaggagg aagaagagag aaaacgagag gaaaacacca 2221 gcaatgaaag cacagatgtg accaagggag acagcaaaaa tgctaaaaag aagaataata 2281 agaaaaccag caaaaataag agcagcctga gtaggggcaa caagaagaaa cccgggatgc 2341 ccaatgtatc taacgacctc tcacagaaac tatatgccac catggagaag cataaagagg 2401 tcttctttgt gatccgcctc attgctggcc ctgctgccaa ctccctgcct cccattgttg 2461 atcctgatcc tctcatcccc tgcgatctga tggatggtcg ggatgcgttt ctcacgctgg 2521 caagggacaa gcacctggag ttctcttcac tccgaagagc ccagtggtcc accatgtgca 2581 tgctggtgga gctgcacacg cagagccagg acggctcgag ctgataagcg gccgcataat 2641 gcttaagtcg aacagaaagt aatcgtattg tacacggccg cataatcgaa attaatacga 2701 ctcactatag gggaattgtg agcggataac aattccccat cttagtatat tagttaagta 2761 taagaaggag atatacaaag gttcatatgg accccttggc agtgtcagca gcttcagtag 2821 tgtcaatgtc caatgacgtt ttgaaaccag agacgcccaa ggggccaatc ataatcagta 2881 aaaacccatc aaatggtatt ttctatggtc cctccttcac taaacgagag tctctcaatg 2941 ctcgaatgtt tctgaaatac tatggtgcac acaaattttt agacacttac ctccccgagg 3001 atttgaactc gttatacatt tactatctta tcaagttgct aggctttgaa gttaaagatc 3061 aagcgcttat cggcaccatc aacagtattg tccatatcaa ctcgcaagag cgtgttcaag 3121 atttgggaag tgcaatatct gtcacaaatg ttgaagaccc attggcaaaa aagcaaacag 3181 ttcgtctaat caaagatttg caaagagcaa ttaacaaagt tctatgtaca agattaagat 3241 tatccaattt tttcactatt gatcatttta ttcaaaaatt acataccgct agaaaaattt 3301 tggtcctgac tggtgcaggt gtttcaactt cattaggcat cccggacttc agatcttctg 3361 aggggttcta ttcaaagatc aaacatttgg ggctcgatga tccccaagac gttttcaatt 3421 acaatatatt tatgcacgac ccctctgttt tctataatat tgccaatatg gttttacctc 3481 cagaaaaaat ttattctcca ttgcatagtt tcattaagat gctacaaatg aaagggaaat 3541 tattgagaaa ttatactcaa aacattgata atttggaatc ttatgcggga ataagcacag 3601 ataaactggt gcagtgccat ggctcttttg ctactgccac ctgcgttacc tgccattgga 3661 acctacccgg tgagaggata tttaataaaa ttagaaacct cgaacttcca ctatgcccgt 3721 actgttacaa aaaaagaaga gaatatttcc cagagggata taataataaa gtaggtgttg 3781 ctgcatcaca gggttcaatg tcggaaaggc ctccatatat ccttaactca tatggcgttc 3841 tcaaaccaga tatcacattc tttggcgaag cactgccaaa taaatttcat aagagcattc 3901 gcgaagatat cttagaatgt gatttgttga tttgcattgg gacaagttta aaagtagcgc 3961 cagtgtctga aatcgtaaac atggttcctt cccacgttcc ccaagtcctg attaatcgtg 4021 atcccgtcaa gcacgcagaa tttgatttat ctcttttggg gtactgtgat gacattgcag 4081 ctatggtagc ccaaaaatgt ggctggacga ttccgcataa gaaatggaac gatttgaaga 4141 acaagaactt taaatgccaa gagaaggata agggcgtgta tgtcgttaca tcagatgaac 4201 atcccaaaac cctctaatga ctcgag //