LOCUS       Exported                5383 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Entry vector encoding non-tagged O-GlcNAc Transferase.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR1A hu_ncOGT WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Levine ZG, Potter SC ,Joiner CM , Fei GQ, Nabet B, Sonnett M,Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian Cell Proliferation Requires Noncatalytic Functions of 
            O-GlcNAc Transferase
  JOURNAL   bioRxiv 2020
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3612..3711)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3823..3842)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3834..4643
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3901..3920)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4733..5321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5222..5241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggtttaa aggaaccaat tcagtcgact
      481 ggatccatgg cgtcttccgt gggcaacgtg gccgacagca cagggttagc tgagttggca
      541 catcgagaat atcaggcagg agattttgag gcagctgaga gacactgcat gcagctctgg
      601 agacaagagc cagacaatac tggtgtgctt ttattacttt catctataca cttccagtgt
      661 cgaaggctgg acagatctgc tcactttagc actctggcaa ttaaacagaa cccccttctg
      721 gcagaagctt attcgaattt ggggaatgtg tacaaggaaa gagggcagtt gcaggaggca
      781 attgagcatt atcgacatgc attgcgtctc aaacctgatt tcatcgatgg ttatattaac
      841 ctggcagccg ccttggtagc agcgggtgac atggaagggg cagtacaagc ttacgtctct
      901 gctcttcagt acaatcctga tttgtactgt gttcgcagtg acctggggaa cctgctcaaa
      961 gccctgggtc gcttggaaga agccaaggca tgttatttga aagcaattga gacgcaaccg
     1021 aactttgcag tagcttggag taatcttggc tgtgttttca atgcacaagg ggaaatttgg
     1081 cttgcaattc atcactttga aaaggctgtc acccttgacc caaactttct ggatgcttat
     1141 atcaatttag gaaatgtctt gaaagaggca cgcatttttg acagagctgt ggcagcttat
     1201 cttcgtgccc taagtttgag tccaaatcac gcagtggtgc acggcaacct ggcttgtgta
     1261 tactatgagc aaggcctgat agatctggca atagacacct acaggcgggc tatcgaacta
     1321 caaccacatt tccctgatgc ttactgcaac ctagccaatg ctctcaaaga gaagggcagt
     1381 gttgctgaag cagaagattg ttataataca gctctccgtc tgtgtcccac ccatgcagac
     1441 tctctgaata acctagccaa tatcaaacga gaacagggaa acattgaaga ggcagttcgc
     1501 ttgtatcgta aagcattaga agtcttccca gagtttgctg ctgcccattc aaatttagca
     1561 agtgtactgc agcagcaggg aaaactgcag gaagctctga tgcattataa ggaggctatt
     1621 cgaatcagtc ctacctttgc tgatgcctac tctaatatgg gaaacactct aaaggagatg
     1681 caggatgttc agggagcctt gcagtgttat acgcgtgcca tccaaattaa tcctgcattt
     1741 gcagatgcac atagcaatct ggcttccatt cataaggatt cagggaatat tccagaagcc
     1801 atagcttctt accgcacggc tctgaaactt aagcctgatt ttcctgatgc ttattgtaac
     1861 ttggctcatt gcctgcagat tgtctgtgat tggacagact atgatgagcg aatgaagaag
     1921 ttggtcagta ttgtggctga ccagttagag aagaataggt tgccttctgt gcatcctcat
     1981 catagtatgc tatatcctct ttctcatggc ttcaggaagg ctattgctga gaggcacggc
     2041 aacctgtgct tagataagat taatgttctt cataaaccac catatgaaca tccaaaagac
     2101 ttgaagctca gtgatggtcg gctgcgtgta ggatatgtga gttccgactt tgggaatcat
     2161 cctacttctc accttatgca gtctattcca ggcatgcaca atcctgataa atttgaggtg
     2221 ttctgttatg ccctgagccc agacgatggc acaaacttcc gagtgaaggt gatggcagaa
     2281 gccaatcatt tcattgatct ttctcagatt ccatgcaatg gaaaagcagc tgatcgcatc
     2341 catcaggatg gaattcatat ccttgtaaat atgaatggct atactaaggg cgctcgaaat
     2401 gagctttttg ctctcaggcc agctcctatt caggcaatgt ggctgggata ccctgggacg
     2461 agtggtgcgc ttttcatgga ttatattatc actgatcagg aaacttcgcc agctgaagtt
     2521 gctgagcagt attccgagaa attggcttat atgccccaca ctttttttat tggtgatcat
     2581 gctaatatgt tccctcacct gaagaaaaaa gcagtcatcg attttaagtc caatgggcac
     2641 atttatgaca atcggatagt tctgaatggc atcgacctca aagcatttct tgatagtcta
     2701 ccagatgtga aaattgtcaa gatgaagtgt cctgatggag gagacaatgc agatagcagt
     2761 aacacagctc ttaatatgcc tgttattcct atgaatacta ttgcagaagc agttattgaa
     2821 atgattaacc gaggacagat tcaaataaca attaatggat tcagtattag caatggactg
     2881 gcaactactc agatcaacaa taaggctgca actggagagg aggttccccg taccattatt
     2941 gtaaccaccc gttctcagta cgggttacca gaagatgcca tcgtatactg taactttaat
     3001 cagttgtata aaattgaccc ttctactttg cagatgtggg caaacattct gaagcgtgtt
     3061 cccaatagtg tactctggct gttgcgtttt ccagcagtag gagaacctaa tattcaacag
     3121 tatgcacaaa acatgggcct gccccagaac cgtatcattt tttcacctgt tgctcctaaa
     3181 gaggaacacg tcaggagagg ccagctggct gatgtctgct tggacactcc actctgtaat
     3241 gggcacacca cagggatgga tgtcctctgg gcagggaccc ccatggtgac tatgccagga
     3301 gagactcttg cttctcgagt tgcagcatcc cagctcactt gcttaggttg tcttgagctt
     3361 attgctaaaa acagacaaga atatgaagac atagctgtga agctgggaac tgatctagaa
     3421 tacctgaaga aagttcgtgg caaagtctgg aagcaaagaa tatctagccc tctgttcaac
     3481 accaaacaat acacaatgga actagagcgg ctctatctac agatgtggga gcattatgca
     3541 gctggcaaca aacctgacca catgattaag cctgttgaag tcactgagtc agcataactc
     3601 gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     3661 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     3721 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     3781 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     3841 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     3901 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     3961 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     4021 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     4081 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     4141 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     4201 cagtgtccct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     4261 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     4321 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     4381 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     4441 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     4501 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     4561 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     4621 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
     4681 gggccccgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4861 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtt
//
