LOCUS       Exported                4730 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Peft-3::wrmScarlet1-10::unc-54 3'UTR. Expresses wrmScarlet1-10 in 
            somatic tissues of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    pJG100
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Goudeau J, Paw, J, Savy L, Leonetti MD, York A, Kenyon C, Ingaramo M
  TITLE     Split-wrmScarlet and split-sfGFP: tools for faster, easier 
            fluorescent labeling of endogenous proteins in Caenorhabditis 
            elegans
  JOURNAL   bioRxiv
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     206..228
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     220..237
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     221..237
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        258..857
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     CDS             885..896
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2396..2412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2396..2412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2409..2431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2420..2436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2444..2474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2489..2510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2627..2644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2798..3386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2878..2897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3557..4372)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4280..4299
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(4373..4477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4545..4563
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4601..4623)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4723..12
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
       61 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      121 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      181 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      241 ttgacgcgta ttgggatgca cctttggtct tttattgtca acttccattg gttcttccat
      301 tgtttctgtt aaattaatga atttttcata aaataaagac attatacaat ataaaaatga
      361 agaatttatt gaaaataaac tgccagagag aaaaagtatg caacactccc gccgagagtg
      421 tttgaaatgg tgtacggtac attttcgtgc taggagttag atgtgcaggc agcaacgaga
      481 gggggagaga tttttttggg ccttgtgaaa ttaacgtgag ttttctggtc atctgactaa
      541 tcatgttggt tttttgttgg tttattttgt ttttatcttt gtttttatcc agattaggaa
      601 atttaaattt tatgaattta taatgaggtc aaacattcag tcccagcgtt tttcctgttc
      661 tcactgttta gtcgaatttt tattttaggc tttcaacaaa tgttctaact gtcttatttg
      721 tgacctcact ttttatattt ttttaatttt taaaaatatt agaagtttct aggataattt
      781 tttcgacttt tattctctct accgtccgca ctcttcttac ttttaaatta aattgttttt
      841 ttttcagttg ggaaacactt tgctcactcc gtagcagcca tggtatcgaa gggagaagca
      901 gtaatcaagg agttcatgcg tttcaaggtc cacatggagg gatccatgaa cggacacgag
      961 ttcgagatcg agggagaggg agagggacgt ccatacgagg gaacccaaac cgccaagctc
     1021 aaggtcacca aggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     1081 cagggaggac cactcccatt ctcctgggac atcctctccc cacaattcat gtacggatcc
     1141 cgtgccttca tcaagcaccc agccgacatc ccagactact acaagcaatc cttcccagag
     1201 ggattcaagt gggagcgtgt catgaacttc gaggacggag gagccgtcac cgtcacccaa
     1261 gacacctccc tcgaggacgg aaccctcatc tacaaggtaa gtttaaacag ttcggtacta
     1321 actaaccata catatttaaa ttttcaggtc aagctccgtg gaaccaactt cccaccagac
     1381 ggaccagtca tgcaaaagaa gaccatggga tgggaggcct ccaccgagcg tctctaccca
     1441 gaggacggag tcctcaaggt aagtttaaac atgattttac taactaacta atctgattta
     1501 aattttcagg gagacatcac catggccctc cgtctcaagg acggaggacg ttacctcgcc
     1561 gacacctcca ccacctacaa ggccaagaag ccagtccaaa tgccaggagc ctacctcgtc
     1621 gaccgtaagc tcgacatcac ctcccacaac gagtactaat aagtccaatt actcttcaac
     1681 atccctacat gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac
     1741 ttctcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     1801 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     1861 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     1921 ttacttctga taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc
     1981 atatgttacg tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca
     2041 tgacgtcaaa tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca
     2101 agtgaaagtt tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt
     2161 gtcaagattt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     2221 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     2281 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     2341 atataccaaa cataactgtt tatcccaatg gcgcgccgag cttggctcga gcatggtcat
     2401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2521 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2581 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     2641 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     2701 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3541 aaacttggtc tgacagttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     3601 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     3661 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     3721 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     3781 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     3841 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     3901 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     3961 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     4021 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccaggg atcgcagtgg
     4081 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     4141 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     4201 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     4261 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     4321 tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc atactcttcc
     4381 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4441 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4501 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     4561 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4621 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4681 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
//
