LOCUS       Exported               11637 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses nCDA1-Delta-198-BE3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    pJT82_GalL_nCDA1-Delta-198-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Engineering of high-precision base editors for site-specific single 
            nucleotide replacement.
  JOURNAL   Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.
  PUBMED    30683865
REFERENCE   2  (bases 1 to 11637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        913..943
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1439..1462
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     1483..1499
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1483..1499
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             2107..6210
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6223..6471
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6484..6504
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6520..6767
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6536..6554)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6785..6804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6786..6804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6814..6831)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6814..6830)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6823..6845)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6971..7426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7058..7077)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7268..7289
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7727..8134
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8135..9229
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9723..9742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9842..9864
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9902..9920)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    9962..10465
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10502..10606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10607..11467
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10825..10844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcgt gacagccctc cgaaggaaga ctctcctccg
     1081 tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc
     1141 tccgaacaat aaagattcta caatactagc ttttatggtt atgaagagga aaaattggca
     1201 gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat taacaaccat aggatgataa
     1261 tgcgattagt tttttagcct tatttctggg gtaattaatc agcgaagcga tgatttttga
     1321 tctattaacg gatatataaa tgcaaaaact gcataaccac tttaactaat actttcaaca
     1381 ttttcggttt gtattacttc ttattcaaat gtaataaaag tatcaacaaa aaattgttaa
     1441 tatacctcta tactttaacg tcaaggagaa aaaaccccgg attctagaac tagtggatcc
     1501 cccgggaaaa aaatgaccga cgctgagtac gtgagaatcc atgagaagtt ggacatctac
     1561 acgtttaaga aacagttttt caacaacaaa aaatccgtgt cgcatagatg ctacgttctc
     1621 tttgaattaa aacgacgggg tgaacgtaga gcgtgttttt ggggctatgc tgtgaataaa
     1681 ccacagagcg ggacagaacg tggcattcac gccgaaatct ttagcattag aaaagtcgaa
     1741 gaatacctgc gcgacaaccc cggacaattc acgataaatt ggtactcatc ctggagtcct
     1801 tgtgcagatt gcgctgaaaa gatcttagaa tggtataacc aggagctgcg ggggaacggc
     1861 cacactttga aaatctgggc ttgcaaactc tattacgaga aaaatgcgag gaatcaaatt
     1921 gggctgtgga acctcagaga taacggggtt gggttgaatg taatggtaag tgaacactac
     1981 caatgttgca ggaaaatatt catccaatcg tcgcacaatc aattgaatga gaatagatgg
     2041 cttgagaaga ctttgaagcg agctgaaaaa cgacggagcg agttgtccat tatgattcag
     2101 gtaaaaatgg acaagaagta ctccattggg ctcgctatcg gcacaaacag cgtcggttgg
     2161 gccgtcatta cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc
     2221 gatcgccaca gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggagacg
     2281 gccgaagcca cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg
     2341 atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc
     2401 cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc
     2461 tttggcaata tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg
     2521 aggaagaagc ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg
     2581 gcgcatatga tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac
     2641 agcgatgtcg acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag
     2701 aacccgatca acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa
     2761 tcccggcggc tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt
     2821 ggtaatctta tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg
     2881 gccgaagatg ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg
     2941 ctggcccaga tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac
     3001 gccattctgc tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc
     3061 gctagtatga tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt
     3121 gtcagacagc aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc
     3181 tacgccggat acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc
     3241 atcttggaaa aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg
     3301 ttgcgcaaac agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa
     3361 ctgcacgcta tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa
     3421 aagattgaga aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga
     3481 aattccagat tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc ctggaacttc
     3541 gaggaagtcg tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt
     3601 gataaaaatc tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc
     3661 acagtttata acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca
     3721 ttcctgtctg gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa
     3781 gttaccgtga aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt
     3841 gaaatcagcg gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg
     3901 aaaatcatta aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac
     3961 attgtcctca cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact
     4021 tacgctcatc tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga
     4081 tgggggcggc tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca
     4141 atcctggatt ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat
     4201 gatgactctc tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac
     4261 agtcttcacg agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg
     4321 cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat
     4381 atcgttatcg agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg
     4441 gaaaggatga agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa
     4501 cacccagttg aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac
     4561 ggcagggaca tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg
     4621 gatcatatcg tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca
     4681 agatccgata aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa
     4741 atgaaaaatt attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat
     4801 aatctgacta aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa
     4861 aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc
     4921 atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg
     4981 aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc
     5041 aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc
     5101 aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt
     5161 aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt
     5221 tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg
     5281 aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg
     5341 gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc
     5401 gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag
     5461 ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca
     5521 gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa
     5581 agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc
     5641 atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt
     5701 cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc
     5761 gagctgcaga aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg
     5821 gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc
     5881 gtggaacaac acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa
     5941 agagtgatcc tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg
     6001 gataagccca tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg
     6061 ggcgcgcctg cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct
     6121 acaaaggagg tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca
     6181 agaatcgacc tctctcagct cggtggagac tctggtggtt caactaattt atctgatatt
     6241 attgaaaaag aaacaggtaa acaattagtt attcaagaat caattttaat gttgccagaa
     6301 gaagttgaag aagttattgg taataaacca gaatctgata ttttagttca tactgcttat
     6361 gatgaatcta ctgatgaaaa tgttatgtta ttgacttcag atgctccaga atataaacca
     6421 tgggctttag ttattcaaga ttctaatggt gaaaataaaa ttaaaatgtt gtctggtggt
     6481 tcacccaaga agaagaggaa ggtgtgatct cttctcgagt catgtaatta gttatgtcac
     6541 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac
     6601 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
     6661 tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga
     6721 aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa
     6781 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6841 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6901 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     6961 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7381 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     7441 ttctccttac gcatctgtgc ggtatttcac accgcatatc gaccggtcga ggagaacttc
     7501 tagtatatct acatacctaa tattattgcc ttattaaaaa tggaatccca acaattacat
     7561 caaaatccac attctcttca aaatcaattg tcctgtactt ccttgttcat gtgtgttcaa
     7621 aaacgttata tttataggat aattatactc tatttctcaa caagtaattg gttgtttggc
     7681 cgagcggtct aaggcgcctg attcaagaaa tatcttgacc gcagttaact gtgggaatac
     7741 tcaggtatcg taagatgcaa gagttcgaat ctcttagcaa ccattatttt tttcctcaac
     7801 ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt
     7861 tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa
     7921 aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta
     7981 aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc
     8041 aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat
     8101 atatatatat atatttcaag gatataccat tctaatgtct gcccctaaga agatcgtcgt
     8161 tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg ttcttaaagc
     8221 tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa ttggtggtgc
     8281 tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct ccaagaaggc
     8341 tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta gtgttagacc
     8401 tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact taagaccatg
     8461 taactttgca tccgactctc ttttagactt atctccaatc aagccacaat ttgctaaagg
     8521 tactgacttc gttgttgtca gagaattagt gggaggtatt tactttggta agagaaagga
     8581 agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag aagtgcaaag
     8641 aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc ctatttggtc
     8701 cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg tggaggaaac
     8761 catcaagaac gaattcccta cattgaaggt tcaacatcaa ttgattgatt ctgccgccat
     8821 gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca acatgtttgg
     8881 tgatatcatc tccgatgaag cctccgttat cccaggttcc ttgggtttgt tgccatctgc
     8941 gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac catgccacgg
     9001 ttctgctcca gatttgccaa agaataaggt caaccctatc gccactatct tgtctgctgc
     9061 aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaggccattg aagatgcagt
     9121 taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca acagtaccac
     9181 cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa aagattctct
     9241 ttttttatga tatttgtaca taaactttat aaatgaaatt cataatagaa acgacacgaa
     9301 attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc tacatacatt
     9361 tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt gatactaatg
     9421 gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa ggaggagggc
     9481 accacacaaa aagttaggtg taacagaaaa tcatgaaact atgattccta atttatatat
     9541 tggaggattt tctctaaaaa aaaaaaaata caacaaataa aaaacactca atgacctgac
     9601 catttgatgg agtttaagtc aataccttct tgaaccattt cccataatgg tgaaagttcc
     9661 ctcaagaatt ttactctgtc agaaacggcc ttaacgacgt agtcgatatg gtgcactctc
     9721 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     9781 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     9841 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     9901 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttaggac
     9961 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga
    10021 atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag
    10081 aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac
    10141 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
    10201 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
    10261 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
    10321 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
    10381 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
    10441 tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt
    10501 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
    10561 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
    10621 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
    10681 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
    10741 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
    10801 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
    10861 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
    10921 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
    10981 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
    11041 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
    11101 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
    11161 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
    11221 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
    11281 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
    11341 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
    11401 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
    11461 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
    11521 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
    11581 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
