LOCUS       Exported               12061 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expressing base editor cCDA1-NGBE3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    pJT131_GalL_cCDA1-NGBE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Expanding the genome-targeting scope and the site selectivity of 
            high-precision base editors.
  JOURNAL   Nat Commun. 2020 Jan 31;11(1):629. doi: 10.1038/s41467-020-14465-z.
  PUBMED    32005820
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        913..943
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1347..1788
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1347..1464
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     1759..1782
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             4385..4396
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5950..6573
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             6592..6840
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6853..6873
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6907..7094
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6962..6986)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      7131..7176
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(7212..7231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7213..7231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7238..7255)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7238..7254)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7247..7269)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7395..7850
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7482..7501)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7692..7713
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8151..8558
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             8559..9653
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(10147..10166)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10266..10288
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10326..10344)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    10386..10889
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        10926..11030
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11031..11891
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11249..11268)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatra ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggtaccggg cccgaattct ctccttctct taggtggcag
     1081 agcaggtgga gggtcgacca tactagtttc aaaaattctt actttttttt tggatggacg
     1141 caaagaagtt taataatcat attacatggc attaccacca tatacatatc catatacata
     1201 tccatataca tatccatata catatccata tctaatctta cttatatgtt gtggaaatgt
     1261 aaagagcccc attatcttag cctaaaaaaa ccttctcttt ggaactttca gtaatacgct
     1321 taactgctca ttgctatatt gaagtacgga ttagaagccg ccgagcgggt gacagccctc
     1381 cgaaggaaga ctctcctccg tgcgtcctcg tcttcaccgg tcgcgttcct gaaacgcaga
     1441 tgtgcctcgc gccgcactgc tccgaacaat aaagattcta caatactagc ttttatggtt
     1501 atgaagagga aaaattggca gtaacctggc cccacaaacc ttcaaatgaa cgaatcaaat
     1561 taacaaccat aggatgataa tgcgattagt tttttagcct tatttctggg gtaattaatc
     1621 agcgaagcga tgatttttga tctattaaca gatatataaa tgcaaaaact gcataaccac
     1681 tttaactaat actttcaaca ttttcggttt gtattacttc ttattcaaat gtaataaaag
     1741 tatcaacaaa aaattgttaa tatacctcta tactttaacg tcaaggagaa aaaacccatg
     1801 gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggttg ggccgtcatt
     1861 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac
     1921 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac ggccgaagcc
     1981 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac
     2041 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg
     2101 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat
     2161 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag
     2221 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg
     2281 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc
     2341 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc
     2401 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg
     2461 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt
     2521 atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat
     2581 gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag
     2641 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg
     2701 ctgagtgata ttctgcgagt gaacacggag atcaccaaaa ctccgctgag cgctagtatg
     2761 atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag
     2821 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga
     2881 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa
     2941 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa
     3001 cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct
     3061 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag
     3121 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga
     3181 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc
     3241 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat
     3301 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat
     3361 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct
     3421 ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg
     3481 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc
     3541 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt
     3601 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc
     3661 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat
     3721 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg
     3781 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
     3841 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct
     3901 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac
     3961 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
     4021 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
     4081 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg
     4141 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
     4201 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
     4261 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc
     4321 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
     4381 aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
     4441 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
     4501 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt
     4561 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     4621 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     4681 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     4741 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     4801 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     4861 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     4921 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     4981 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     5041 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     5101 accggaggct tctccaagga aagtatccgc ccgaaaagga acagcgacaa gctgatcgca
     5161 cgcaaaaaag attgggaccc caagaaatac ggcggattcg tttctcctac agtcgcttac
     5221 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     5281 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     5341 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     5401 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcgcg cttcctgcag
     5461 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     5521 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     5581 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     5641 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     5701 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     5761 cgagccttca agtacttcga caccaccata gacagaaagg tatacagatc tacaaaggag
     5821 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     5881 ctctctcagc tcggtggaga ctctggttct gaaactccag gtacttctga atctgctact
     5941 ccagaatcta tgaccgacgc tgagtacgtg agaatccatg agaagttgga catctacacg
     6001 tttaagaaac agtttttcaa caacaaaaaa tccgtgtcgc atagatgcta cgttctcttt
     6061 gaattaaaac gacggggtga acgtagagcg tgtttttggg gctatgctgt gaataaacca
     6121 cagagcggga cagaacgtgg cattcacgcc gaaatcttta gcattagaaa agtcgaagaa
     6181 tacctgcgcg acaaccccgg acaattcacg ataaattggt actcatcctg gagtccttgt
     6241 gcagattgcg ctgaaaagat cttagaatgg tataaccagg agctgcgggg gaacggccac
     6301 actttgaaaa tctgggcttg caaactctat tacgagaaaa atgcgaggaa tcaaattggg
     6361 ctgtggaacc tcagagataa cggggttggg ttgaatgtaa tggtaagtga acactaccaa
     6421 tgttgcagga aaatattcat ccaatcgtcg cacaatcaat tgaatgagaa tagatggctt
     6481 gagaagactt tgaagcgagc tgaaaaacga cggagcgagt tgtccattat gattcaggta
     6541 aaaatactcc acaccactaa gagtcctgct gtttctagat ctggtggttc aactaattta
     6601 tctgatatta ttgaaaaaga aacaggtaaa caattagtta ttcaagaatc aattttaatg
     6661 ttgccagaag aagttgaaga agttattggt aataaaccag aatctgatat tttagttcat
     6721 actgcttatg atgaatctac tgatgaaaat gttatgttat tgacttcaga tgctccagaa
     6781 tataaaccat gggctttagt tattcaagat tctaatggtg aaaataaaat taaaatgttg
     6841 tctggtggtt cacccaagaa gaagaggaag gtgtgatcta gaggctccgg ataaggatcc
     6901 taataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     6961 agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt
     7021 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     7081 cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctcccgggca aaaaaccccc
     7141 tcaagacccg tttagaggcc ccaaggggtt atgctatgca taccaagctt ccgcggtgga
     7201 gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     7261 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7321 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7381 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7441 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7501 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7561 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7621 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7681 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7741 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7801 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
     7861 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatcgaccgg tcgaggagaa
     7921 cttctagtat atctacatac ctaatattat tgccttatta aaaatggaat cccaacaatt
     7981 acatcaaaat ccacattctc ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt
     8041 tcaaaaacgt tatatttata ggataattat actctatttc tcaacaagta attggttgtt
     8101 tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt aactgtggga
     8161 atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct
     8221 caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat
     8281 tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag
     8341 aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg
     8401 actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt
     8461 ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag
     8521 caatatatat atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg
     8581 tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta
     8641 aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg
     8701 gtgctgctat cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga
     8761 aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta
     8821 gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac
     8881 catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta
     8941 aaggtactga cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa
     9001 aggaagacga tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc
     9061 aaagaatcac aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt
     9121 ggtccttgga taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg
     9181 aaaccatcaa gaacgagttc cctacattga aggttcaaca tcaattgatt gattctgccg
     9241 ccatgatcct agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt
     9301 ttggtgatat catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat
     9361 ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc
     9421 acggttctgc tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg
     9481 ctgcaatgat gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg
     9541 cagttaaaaa ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta
     9601 ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt
     9661 ctcttttttt atgatatttg tacataaact ttataaatga aattcataat agaaacgaca
     9721 cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata
     9781 catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact
     9841 aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga
     9901 gggcaccaca caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat
     9961 atattggagg attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc
    10021 tgaccatttg atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag
    10081 ttccctcaag aattttactc tgtcagaaac ggccttaacg acgtagtcga tatggtgcac
    10141 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
    10201 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    10261 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
    10321 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
    10381 ggacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt
    10441 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat
    10501 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
    10561 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
    10621 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
    10681 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag
    10741 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
    10801 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
    10861 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
    10921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
    10981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
    11041 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
    11101 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    11161 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    11221 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    11281 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    11341 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    11401 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    11461 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    11521 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    11581 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    11641 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    11701 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    11761 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    11821 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    11881 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    11941 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    12001 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    12061 c
//
