LOCUS Exported 6125 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli. ACCESSION . VERSION . KEYWORDS pEvol-MjaYRS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6125) AUTHORS Zhang S, Ai HW TITLE A general strategy to red-shift green fluorescent protein-based biosensors. JOURNAL Nat Chem Biol. 2020 Sep 14. pii: 10.1038/s41589-020-0641-7. doi: 10.1038/s41589-020-0641-7. PUBMED 32929278 REFERENCE 2 (bases 1 to 6125) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6125 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 7..24 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(80..97) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" CDS complement(2000..2659) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2574..2593 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" promoter complement(2660..2762) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" primer_bind complement(3154..3171) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3288..3833) /direction=LEFT /label=p15A ori /note="Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin. " primer_bind complement(3368..3387) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3979..4857) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" promoter 4884..5168 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 5091..5110 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" ORIGIN 1 gtcgaccatc atcatcatca tcattgagtt taaacggtct ccagcttggc tgttttggcg 61 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa 121 aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag 181 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact 241 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggccttgtt tgtgagctcc 301 cggtcatcaa tcatccccat aatccttgtt agattatcaa ttttaaaaaa ctaacagttg 361 tcagcctgtc ccgctttaat atcatacgcc gttatacgtt gtttacgctt tgaggaatcc 421 catatggacg aatttgaaat gataaagaga aacacatctg aaattatcag cgaggaagag 481 ttaagagagg ttttaaaaaa agatgaaaaa tctgcttaca taggttttga accaagtggt 541 aaaatacatt tagggcatta tctccaaata aaaaagatga ttgatttaca aaatgctgga 601 tttgatataa ttatattgtt ggctgattta agcgcctatt taaaccagaa aggagagttg 661 gatgagatta gaaaaatagg agattataac aaaaaagttt ttgaagcaat ggggttaaag 721 gcaaaatatg tttatggaag tgaattccag cttgataagg attatacact gaatgtctat 781 agattggctt taaaaactac cttaaaaaga gcaagaagga gtatggaact tatagcaaga 841 gaggatgaaa atccaaaggt tgctgaagtt atctatccaa taatgcaggt taatactagc 901 cattatttag gcgttgatgt tgcagttgga gggatggagc agagaaaaat acacatgtta 961 gcaagggagc ttttaccaaa aaaggttgtt tgtattcaca accctgtctt aacgggtttg 1021 gatggagaag gaaagatgag ttcttcaaaa gggaatttta tagctgttga tgactctcca 1081 gaagagatta gggctaagat aaagaaagca tactgcccag ctggagttgt tgaaggaaat 1141 ccaataatgg agatagctaa atatttcctt gaatatcctt taaccataaa aaggccagaa 1201 aaatttggtg gagatttgac agttaatagc tatgaggagt tagagagttt atttaaaaat 1261 aaggaattgc atccaatgta tttaaaaaat gctgtagctg aagaacttat aaagatttta 1321 gagccaatta gaaagagact gtaactgcag tttcaaacgc taaattgcct gatgcgctac 1381 gcttatcagg cctacatgat ctctgcaata tattgagttt gcgtgctttt gtaggccgga 1441 taaggcgttc acgccgcatc cggcaagaaa cagcaaacaa tccaaaacgc cgcgttcagc 1501 ggcgtttttt ctgcttttct tcgcgaatta attccgcttc gcaacatgtg agcaccggtt 1561 tattgactac cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc 1621 aggctctccc cgtggaggta ataattgacg atatgatcag tgcacggcta actaagcggc 1681 ctgctgactt tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat 1741 ctgcgcagta agatgcgccc cgcattccgg cggtagttca gcagggcaga acggcggact 1801 ctaaatccgc atggcagggg ttcaaatccc ctccgccgga ccaaattcga aaagcctgct 1861 caacgagcag gcttttttgc atgctcgagc agctcagggt cgaatttgct ttcgaatttc 1921 tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc 1981 aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt 2041 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca 2101 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga 2161 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg 2221 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt 2281 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac 2341 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac 2401 tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga tgagcattca 2461 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg 2521 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg 2581 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc 2641 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa 2701 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat 2761 caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag 2821 gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt 2881 cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg 2941 gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc 3001 aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg 3061 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca 3121 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt 3181 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc 3241 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc 3301 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca 3361 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct 3421 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct 3481 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt 3541 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca 3601 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt 3661 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc 3721 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg 3781 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc 3841 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag 3901 cacctgaagt cagccccata cgatataagt tgtaattctc atgtttgaca gcttatcatc 3961 gataagcttg gtacccaatt atgacaactt gacggctaca tcattcactt tttcttcaca 4021 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata 4081 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct 4141 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc 4201 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac 4261 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 4321 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 4381 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 4441 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 4501 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 4561 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 4621 aatctctcct ggcgggaaca gcaaaatatc actcggtcgg caaacaaatt ctcgtccctg 4681 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 4741 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac 4801 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact 4861 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg 4921 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag 4981 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa 5041 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc 5101 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt 5161 ttctccatac ccgttttttt gggctaacag gaggaattag atctatggac gaatttgaaa 5221 tgataaagag aaacacatct gaaattatca gcgaggaaga gttaagagag gttttaaaaa 5281 aagatgaaaa atctgcttac ataggttttg aaccaagtgg taaaatacat ttagggcatt 5341 atctccaaat aaaaaagatg attgatttac aaaatgctgg atttgatata attatattgt 5401 tggctgattt aagcgcctat ttaaaccaga aaggagagtt ggatgagatt agaaaaatag 5461 gagattataa caaaaaagtt tttgaagcaa tggggttaaa ggcaaaatat gtttatggaa 5521 gtgaattcca gcttgataag gattatacac tgaatgtcta tagattggct ttaaaaacta 5581 ccttaaaaag agcaagaagg agtatggaac ttatagcaag agaggatgaa aatccaaagg 5641 ttgctgaagt tatctatcca ataatgcagg ttaatactag ccattattta ggcgttgatg 5701 ttgcagttgg agggatggag cagagaaaaa tacacatgtt agcaagggag cttttaccaa 5761 aaaaggttgt ttgtattcac aaccctgtct taacgggttt ggatggagaa ggaaagatga 5821 gttcttcaaa agggaatttt atagctgttg atgactctcc agaagagatt agggctaaga 5881 taaagaaagc atactgccca gctggagttg ttgaaggaaa tccaataatg gagatagcta 5941 aatatttcct tgaatatcct ttaaccataa aaaggccaga aaaatttggt ggagatttga 6001 cagttaatag ctatgaggag ttagagagtt tatttaaaaa taaggaattg catccaatgt 6061 atttaaaaaa tgctgtagct gaagaactta taaagatttt agagccaatt agaaagagat 6121 tataa //