LOCUS Exported 7582 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays. ACCESSION . VERSION . KEYWORDS pDEST-ERBB3-v2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7582) TITLE A feed-forward loop between SorLA and HER3 determines heregulin response and neratinib resistance JOURNAL Unpublished REFERENCE 2 (bases 1 to 7582) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7582 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 942..966 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4993..5017) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind 5240..5261 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(5338..5355) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5344..5568 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(5656..5673) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5827..6415) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5907..5926) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6586..7446) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7209..7228 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(7447..7551) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccatattacg gggtactcga gacaagtttg tacaaaaaag 961 ttggcaccat gagggcgaac gacgctctgc aggtgctggg cttgcttttc agcctggccc 1021 ggggctccga ggtgggcaac tctcaggcag tgtgtcctgg gactctgaat ggcctgagtg 1081 tgaccggcga tgctgagaac caataccaga cactgtacaa gctctacgag aggtgtgagg 1141 tggtgatggg gaaccttgag attgtgctca cgggacacaa tgccgacctc tccttcctgc 1201 agtggattcg agaagtgaca ggctatgtcc tcgtggccat gaatgaattc tctactctac 1261 cattgcccaa cctccgcgtg gtgcgaggga cccaggtcta cgatgggaag tttgccatct 1321 tcgtcatgtt gaactataac accaactcca gccacgctct gcgccagctc cgcttgactc 1381 agctcaccga gattctgtca gggggtgttt atattgagaa gaacgataag ctttgtcaca 1441 tggacacaat tgactggagg gacatcgtga gggaccgaga tgctgagata gtggtgaagg 1501 acaatggcag aagctgtccc ccctgtcatg aggtttgcaa ggggcgatgc tggggtcctg 1561 gatcagaaga ctgccagaca ttgaccaaga ccatctgtgc tcctcagtgt aatggtcact 1621 gctttgggcc caaccccaac cagtgctgcc atgatgagtg tgccgggggc tgctcaggcc 1681 ctcaggacac agactgcttt gcctgccggc acttcaatga cagtggagcc tgtgtacctc 1741 gctgtccaca gcctcttgtc tacaacaagc taactttcca gctggaaccc aatccccaca 1801 ccaagtatca gtatggagga gtttgtgtag ccagctgtcc ccataacttt gtggtggatc 1861 aaacatcctg tgtcagggcc tgtcctcctg acaagatgga agtagataaa aatgggctca 1921 agatgtgtga gccttgtggg ggactatgtc ccaaagcctg tgagggaaca ggctctggga 1981 gccgcttcca gactgtggac tcgagcaaca ttgatggatt tgtgaactgc accaagatcc 2041 tgggcaacct ggactttctg atcaccggcc tcaatggaga cccctggcac aagatccctg 2101 ccctggaccc agagaagctc aatgtcttcc ggacagtacg ggagatcaca ggttacctga 2161 acatccagtc ctggccgccc cacatgcaca acttcagtgt tttttccaat ttgacaacca 2221 ttggaggcag aagcctctac aaccggggct tctcattgtt gatcatgaag aacttgaatg 2281 tcacatctct gggcttccga tccctgaagg aaattagtgc tgggcgtatc tatataagtg 2341 ccaataggca gctctgctac caccactctt tgaactggac caaggtgctt cgggggccta 2401 cggaagagcg actagacatc aagcataatc ggccgcgcag agactgcgtg gcagagggca 2461 aagtgtgtga cccactgtgc tcctctgggg gatgctgggg cccaggccct ggtcagtgct 2521 tgtcctgtcg aaattatagc cgaggaggtg tctgtgtgac ccactgcaac tttctgaatg 2581 gggagcctcg agaatttgcc catgaggccg aatgcttctc ctgccacccg gaatgccaac 2641 ccatggaggg cactgccaca tgcaatggct cgggctctga tacttgtgct caatgtgccc 2701 attttcgaga tgggccccac tgtgtgagca gctgccccca tggagtccta ggtgccaagg 2761 gcccaatcta caagtaccca gatgttcaga atgaatgtcg gccctgccat gagaactgca 2821 cccaggggtg taaaggacca gagcttcaag actgtttagg acaaacactg gtgctgatcg 2881 gcaaaaccca tctgacaatg gctttgacag tgatagcagg attggtagtg attttcatga 2941 tgctgggcgg cacttttctc tactggcgtg ggcgccggat tcagaataaa agggctatga 3001 ggcgatactt ggaacggggt gagagcatag agcctctgga ccccagtgag aaggctaaca 3061 aagtcttggc cagaatcttc aaagagacag agctaaggaa gcttaaagtg cttggctcgg 3121 gtgtctttgg aactgtgcac aaaggagtgt ggatccctga gggtgaatca atcaagattc 3181 cagtctgcat taaagtcatt gaggacaaga gtggacggca gagttttcaa gctgtgacag 3241 atcatatgct ggccattggc agcctggacc atgcccacat tgtaaggctg ctgggactat 3301 gcccagggtc atctctgcag cttgtcactc aatatttgcc tctgggttct ctgctggatc 3361 atgtgagaca acaccggggg gcactggggc cacagctgct gctcaactgg ggagtacaaa 3421 ttgccaaggg aatgtactac cttgaggaac atggtatggt gcatagaaac ctggctgccc 3481 gaaacgtgct actcaagtca cccagtcagg ttcaggtggc agattttggt gtggctgacc 3541 tgctgcctcc tgatgataag cagctgctat acagtgaggc caagactcca attaagtgga 3601 tggcccttga gagtatccac tttgggaaat acacacacca gagtgatgtc tggagctatg 3661 gtgtgacagt ttgggagttg atgaccttcg gggcagagcc ctatgcaggg ctacgattgg 3721 ctgaagtacc agacctgcta gagaaggggg agcggttggc acagccccag atctgcacaa 3781 ttgatgtcta catggtgatg gtcaagtgtt ggatgattga tgagaacatt cgcccaacct 3841 ttaaagaact agccaatgag ttcaccagga tggcccgaga cccaccacgg tatctggtca 3901 taaagagaga gagtgggcct ggaatagccc ctgggccaga gccccatggt ctgacaaaca 3961 agaagctaga ggaagtagag ctggagccag aactagacct agacctagac ttggaagcag 4021 aggaggacaa cctggcaacc accacactgg gctccgccct cagcctacca gttggaacac 4081 ttaatcggcc acgtgggagc cagagccttt taagtccatc atctggatac atgcccatga 4141 accagggtaa tcttggggag tcttgccagg agtctgcagt ttctgggagc agtgaacggt 4201 gcccccgtcc agtctctcta cacccaatgc cacggggatg cctggcatca gagtcatcag 4261 aggggcatgt aacaggctct gaggctgagc tccaggagaa agtgtcaatg tgtaggagcc 4321 ggagcaggag ccggagccca cggccacgcg gagatagcgc ctaccattcc cagcgccaca 4381 gtctgctgac tcctgttacc ccactctccc cacccgggtt agaggaagag gatgtcaacg 4441 gttatgtcat gccagataca cacctcaaag gtactccctc ctcccgggaa ggcacccttt 4501 cttcagtggg tctcagttct gtcctgggta ctgaagaaga agatgaagat gaggagtatg 4561 aatacatgaa ccggaggaga aggcacagtc cacctcatcc ccctaggcca agttcccttg 4621 aggagctggg ttatgagtac atggatgtgg ggtcagacct cagtgcctct ctgggcagca 4681 cacagagttg cccactccac cctgtaccca tcatgcccac tgcaggcaca actccagatg 4741 aagactatga atatatgaat cggcaacgag atggaggtgg tcctgggggt gattatgcag 4801 ccatgggggc ctgcccagca tctgagcaag ggtatgaaga gatgagagct tttcaggggc 4861 ctggacatca ggccccccat gtccattatg cccgcctaaa aactctacgt agcttagagg 4921 ctacagactc tgcctttgat aaccctgatt actggcatag caggcttttc cccaaggcta 4981 atgcccagag aacgccaact ttcttgtaca aagtggtcat cgatggtggc ggtggctctg 5041 gaggtggtgg gtcctccgga aagaacggca tcaaggccaa cttcaagatc cgccacaaca 5101 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 5161 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc 5221 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 5281 tcggcatgga cgagctgtac aagtaatcta gagggcccgt ttaaacccgc tgatcagcct 5341 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 5401 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 5461 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 5521 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 5581 aaagaaccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 5641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 6421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 6721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 7561 ccgaaaagtg ccacctgacg tc //