LOCUS       Exported                7549 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses ERBB2 fused to Venus fragment 1 (v1) for use in 
            bimolecular fluorescence complementation (BiFC) assays.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-ERBB2-v1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7549)
  TITLE     A feed-forward loop between SorLA and HER3 determines heregulin 
            response and neratinib resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    942..966
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4732..4756)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4800..5261
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(4842..4863)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5103..5122)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(5305..5322)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5311..5535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5623..5640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5794..6382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5874..5893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6553..7413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7176..7195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7414..7518)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatattacg gggtactcga gacaagtttg tacaaaaaag
      961 ttggcaccat ggagctggcg gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc
     1021 ccggagccgc gagcacccaa gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca
     1081 gtcccgagac ccacctggac atgctccgcc acctctacca gggctgccag gtggtgcagg
     1141 gaaacctgga actcacctac ctgcccacca atgccagcct gtccttcctg caggatatcc
     1201 aggaggtgca gggctacgtg ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga
     1261 ggctgcggat tgtgcgaggc acccagctct ttgaggacaa ctatgccctg gccgtgctag
     1321 acaatggaga cccgctgaac aataccaccc ctgtcacagg ggcctcccca ggaggcctgc
     1381 gggagctgca gcttcgaagc ctcacagaga tcttgaaagg aggggtcttg atccagcgga
     1441 acccccagct ctgctaccag gacacgattt tgtggaagga catcttccac aagaacaacc
     1501 agctggctct cacactgata gacaccaacc gctctcgggc ctgccacccc tgttctccga
     1561 tgtgtaaggg ctcccgctgc tggggagaga gttctgagga ttgtcagagc ctgacgcgca
     1621 ctgtctgtgc cggtggctgt gcccgctgca aggggccact gcccactgac tgctgccatg
     1681 agcagtgtgc tgccggctgc acgggcccca agcactctga ctgcctggcc tgcctccact
     1741 tcaaccacag tggcatctgt gagctgcact gcccagccct ggtcacctac aacacagaca
     1801 cgtttgagtc catgcccaat cccgagggcc ggtatacatt cggcgccagc tgtgtgactg
     1861 cctgtcccta caactacctt tctacggacg tgggatcctg caccctcgtc tgccccctgc
     1921 acaaccaaga ggtgacagca gaggatggaa cacagcggtg tgagaagtgc agcaagccct
     1981 gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg agaggtgagg gcagttacca
     2041 gtgccaatat ccaggagttt gctggctgca agaagatctt tgggagcctg gcatttctgc
     2101 cggagagctt tgatggggac ccagcctcca acactgcccc gctccagcca gagcagctcc
     2161 aagtgtttga gactctggaa gagatcacag gttacctata catctcagca tggccggaca
     2221 gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat ccggggacga attctgcaca
     2281 atggcgccta ctcgctgacc ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac
     2341 tgagggaact gggcagtgga ctggccctca tccaccataa cacccacctc tgcttcgtgc
     2401 acacggtgcc ctgggaccag ctctttcgga acccgcacca agctctgctc cacactgcca
     2461 accggccaga ggacgagtgt gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag
     2521 ggcactgctg gggtccaggg cccacccagt gtgtcaactg cagccagttc cttcggggcc
     2581 aggagtgcgt ggaggaatgc cgagtactgc aggggctccc cagggagtat gtgaatgcca
     2641 ggcactgttt gccgtgccac cctgagtgtc agccccagaa tggctcagtg acctgttttg
     2701 gaccggaggc tgaccagtgt gtggcctgtg cccactataa ggaccctccc ttctgcgtgg
     2761 cccgctgccc cagcggtgtg aaacctgacc tctcctacat gcccatctgg aagtttccag
     2821 atgaggaggg cgcatgccag ccttgcccca tcaactgcac ccactcctgt gtggacctgg
     2881 atgacaaggg ctgccccgcc gagcagagag ccagccctct gacgtccatc atctctgcgg
     2941 tggttggcat tctgctggtc gtggtcttgg gggtggtctt tgggatcctc atcaagcgac
     3001 ggcagcagaa gatccggaag tacacgatgc ggagactgct gcaggaaacg gagctggtgg
     3061 agccgctgac acctagcgga gcgatgccca accaggcgca gatgcggatc ctgaaagaga
     3121 cggagctgag gaaggtgaag gtgcttggat ctggcgcttt tggcacagtc tacaagggca
     3181 tctggatccc tgatggggag aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa
     3241 acacatcccc caaagccaac aaagaaatct tagacgaagc atacgtgatg gctggtgtgg
     3301 gctccccata tgtctcccgc cttctgggca tctgcctgac atccacggtg cagctggtga
     3361 cacagcttat gccctatggc tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg
     3421 gctcccagga cctgctgaac tggtgtatgc agattgccaa ggggatgagc tacctggagg
     3481 atgtgcggct cgtacacagg gacttggccg ctcggaacgt gctggtcaag agtcccaacc
     3541 atgtcaaaat tacagacttc gggctggctc ggctgctgga cattgacgag acagagtacc
     3601 atgcagatgg gggcaaggtg cccatcaagt ggatggcgct ggagtccatt ctccgccggc
     3661 ggttcaccca ccagagtgat gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt
     3721 ttggggccaa accttacgat gggatcccag cccgggagat ccctgacctg ctggaaaagg
     3781 gggagcggct gccccagccc cccatctgca ccattgatgt ctacatgatc atggtcaaat
     3841 gttggatgat tgactctgaa tgtcggccaa gattccggga gttggtgtct gaattctccc
     3901 gcatggccag ggacccccag cgctttgtgg tcatccagaa tgaggacttg ggcccagcca
     3961 gtcccttgga cagcaccttc taccgctcac tgctggagga cgatgacatg ggggacctgg
     4021 tggatgctga ggagtatctg gtaccccagc agggcttctt ctgtccagac cctgccccgg
     4081 gcgctggggg catggtccac cacaggcacc gcagctcatc taccaggagt ggcggtgggg
     4141 acctgacact agggctggag ccctctgaag aggaggcccc caggtctcca ctggcaccct
     4201 ccgaaggggc tggctccgat gtatttgatg gtgacctggg aatgggggca gccaaggggc
     4261 tgcaaagcct ccccacacat gaccccagcc ctctacagcg gtacagtgag gaccccacag
     4321 tacccctgcc ctctgagact gatggctacg ttgcccccct gacctgcagc ccccagcctg
     4381 aatatgtgaa ccagccagat gttcggcccc agcccccttc gccccgagag ggccctctgc
     4441 ctgctgcccg acctgctggt gccactctgg aaaggcccaa gactctctcc ccagggaaga
     4501 atggggtcgt caaagacgtt tttgcctttg ggggtgccgt ggagaacccc gagtacttga
     4561 caccccaggg aggagctgcc cctcagcccc accctcctcc tgccttcagc ccagccttcg
     4621 acaacctcta ttactgggac caggacccac cagagcgggg ggctccaccc agcaccttca
     4681 aagggacacc tacggcagag aacccagagt acctgggtct ggacgtgcca gtgccaactt
     4741 tcttgtacaa agtggtcatc gatggtggcg gtggctctgg aggtggtggg tcctccggag
     4801 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4861 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4921 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4981 tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     5041 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     5101 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     5161 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     5221 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag taatctagag
     5281 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5341 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5401 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5461 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5521 cggtgggctc tatggcttct gaggcggaaa gaaccagctg cattaatgaa tcggccaacg
     5581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5641 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5701 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
