LOCUS Exported 10355 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR. ACCESSION . VERSION . KEYWORDS pGBW-m4092686 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10355) TITLE SARS-CoV-2 protein E. coli expression constructs, tagged, for protein expression and/or purification REFERENCE 2 (bases 1 to 10355) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10355 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 7858..7905 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 7948..7979 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 7980..8082 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 8083..8943 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8301..8320) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" CDS 8944..9135 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" terminator 9159..9186 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(9198..9785) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator 9807..9836 /label=T3Te terminator /note="phage T3 early transcription terminator" promoter 9875..9892 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 atggaaagtg agttcagagt ttattctagt gcgaataatt gcacttttga atatgtctct 61 cagccttttc ttatggacct tgaaggaaaa cagggtaatt tcaaaaatct tagggaattt 121 gtgtttaaga atattgatgg ttattttaaa atatattcta agcacacgcc tattaattta 181 gtgcgtgatc tccctcaggg tttttcggct ttagaaccat tggtagattt gccaataggt 241 attaacatca ctaggtttca aactttactt gctttacata gaagttattt gactcctggt 301 gattcttctt caggttggac agctggtgct gcagcttatt atgtgggtta tcttcaacct 361 aggacttttc tattaaaata taatgaaaat ggaaccatta cagatgctgt agactgtgca 421 cttgaccctc tctcagaaac aaagtgtacg ttgaaatcct tcactgtaga aaaaggaatc 481 tatcaaactt ctaactttag agtccaacca acagaatcta ttgttagatt tcctaatatt 541 acaaacttgt gcccttttgg tgaagttttt aacgccacca gatttgcatc tgtttatgct 601 tggaacagga agagaatcag caactgtgtt gctgattatt ctgtcctata taattccgca 661 tcattttcca cttttaagtg ttatggagtg tctcctacta aattaaatga tctctgcttt 721 actaatgtct atgcagattc atttgtaatt agaggtgatg aagtcagaca aatcgctcca 781 gggcaaactg gaaagattgc tgattataat tataaattac cagatgattt tacaggctgc 841 gttatagctt ggaattctaa caatcttgat tctaaggttg gtggtaatta taattacctg 901 tatagattgt ttaggaagtc taatctcaaa ccttttgaga gagatatttc aactgaaatc 961 tatcaggccg gtagcacacc ttgtaatggt gttgaaggtt ttaattgtta ctttccttta 1021 caatcatatg gtttccaacc cactaatggt gttggttacc aaccatacag agtagtagta 1081 ctttcttttg aacttctaca tgcaccagca actgtttgtg gacctaaaaa gtctactaat 1141 ttggttaaaa acaaatgtgt caatttcaac ttcaatggtt taacaggcac aggtgttctt 1201 actgagtcta acaaaaagtt tctgcctttc caacaatttg gcagagacat tgctgacact 1261 actgatgctg tccgtgatcc acagacactt gagattcttg acattacacc atgttctttt 1321 ggtggtgtca gtgttataac accaggaaca aatacttcta accaggttgc tgttctttat 1381 caggatgtta actgcacaga agtccctgtt gctattcatg cagatcaact tactcctact 1441 tggcgtgttt attctacagg ttctaatgtt tttcaaacac gtgcaggctg tttaataggg 1501 gctgaacatg tcaacaactc atatgagtgt gacataccca ttggtgcagg tatatgcgct 1561 agttatcaga ctcagactaa ttctcctcgg cgggcacgta gtgtagctag tcaatccatc 1621 attgcctaca ctatgtcact tggtgcagaa aattcagttg cttactctaa taactctatt 1681 gccataccca caaattttac tattagtgtt accacagaaa ttctaccagt gtctatgacc 1741 aagacatcag tagattgtac aatgtacatt tgtggtgatt caactgaatg cagcaatctt 1801 ttgttgcaat atggcagttt ttgtacacaa ttaaaccgtg ctttaactgg aatagctgtt 1861 gaacaagaca aaaacaccca agaagttttt gcacaagtca aacaaattta caaaacacca 1921 ccaattaaag attttggtgg ttttaatttt tcacaaatat taccagatcc atcaaaacca 1981 agcaagaggt catttattga agatctactt ttcaacaaag tgacacttgc agatgctggc 2041 ttcatcaaac aatatggtga ttgccttggt gatattgctg ctagggacct catttgtgca 2101 caaaagttta acggccttac tgttttgcca cctttgctca cagatgaaat gattgctcaa 2161 tacacttctg cactgttagc gggtacaatc acttctggtt ggacctttgg tgcaggagct 2221 gcattacaaa taccatttgc tatgcaaatg gcttataggt ttaatggtat tggagttaca 2281 cagaatgttc tctatgagaa ccaaaaattg attgccaacc aatttaatag tgctattggc 2341 aaaattcaag actcactttc ttccacagca agtgcacttg gaaaacttca agatgtggtc 2401 aaccaaaatg cacaagcttt aaacacgctt gttaaacaac ttagctccaa ttttggtgca 2461 atttcaagtg ttttaaatga tatcctttca cgtcttgaca aagttgaggc tgaagtgcaa 2521 attgataggt tgatcacagg cagacttcaa agtttgcaga catatgtgac tcaacaatta 2581 attagagctg cagaaatcag agcttctgct aatcttgctg ctactaaaat gtcagagtgt 2641 gtacttggac aatcaaaaag agttgatttt tgtggaaagg gctatcatct tatgtccttc 2701 cctcagtcag cacctcatgg tgtagtcttc ttgcatgtga cttatgtccc tgcacaagaa 2761 aagaacttca caactgctcc tgccatttgt catgatggaa aagcacactt tcctcgtgaa 2821 ggtgtctttg tttcaaatgg cacacactgg tttgtaacac aaaggaattt ttatgaacca 2881 caaatcatta ctacagacaa cacatttgtg tctggtaact gtgatgttgt aataggaatt 2941 gtcaacaaca cagtttatga tcctttgcaa cctgaattag actcattcaa ggaggagtta 3001 gataaatatt ttaagaatca tacatcacca gatgttgatt taggtgacat ctctggcatt 3061 aatgcttcag ttgtaaacat tcaaaaagaa attgaccgcc tcaatgaggt tgccaagaat 3121 ttaaatgaat ctctcatcga tctccaagaa cttggaaagt atgagcagta tataaaatgg 3181 ccatggtaca tttggctagg ttttatagct ggcttgattg ccatagtaat ggtgacaatt 3241 atgctttgct gtatgaccag ttgctgtagt tgtctcaagg gctgttgttc ttgtggatcc 3301 tgctgcaaat ttgatgaaga cgactctgag ccagtgctca aaggagtcaa attacattac 3361 acataaacga acttatggat ttgtttatga gaatcttcac aattggaact tagactttga 3421 agcaaggtga aatcaaggat gctactcctt cagattttgt tcgcgctact gcaacgatac 3481 cgatacaagc ctcactccct ttcggatggc ttattgttgg cgttgcactt cttgctgttt 3541 ttcagagcgc ttccaaaatc ataaccctca aaaagagatg gcaactagca ctctccaagg 3601 gtgttcactt tgtttgcaac ttgctgttgt tgtttgtaac agtttactca caccttttgc 3661 tcgttgctgc tggccttgaa gccccttttc tctatcttta tgctttagtc tacttcttgc 3721 agagtataaa ctttgtaaga ataataatga ggctttggct ttgctggaaa tgccgttcca 3781 aaaacccatt actttatgat gccaactatt ttctttgctg gcatactaat tgttacgact 3841 attgtatacc ttacaatagt gtaacttctt caattgtcat tacttcaggt gatggcacaa 3901 caagtcctat ttctgaacat gactaccaga ttggtggtta tactgaaaaa tgggaatctg 3961 gagtaaaaga ctgtgttgta ttacacagtt acttcacttc agactattac cagctgtact 4021 caactcaatt gagtacagac actggtgttg aacatgttac cttcttcatc tacaataaaa 4081 ttgttgatga gcctgaagaa catgtccaaa ttcacacaat cgacggttca tccggagttg 4141 ttaatccagt aatggaacca atttatgatg aaccgacgac gactactagc gtgcctttgt 4201 aagcacaagc tgatgagtac gaacttatgt actcattcgt ttcggaagag acaggtacgt 4261 taatagttta aagcgtactt ctttttcttg ctttcgtggt attcttgcta gttacactag 4321 ccatccttac tgcgcttcga ttgtgtgcgt actgctgcaa tattgttaac gtgagtcttg 4381 taaaaccttc tttttacgtt tactctcgtg ttaaaaatct gaattcttct agagttcctg 4441 atcttctggt ctaaacgaac taaatattat attagttttt ctgtttggaa ctttaatttt 4501 agccatggca gattccaacg gtactattac cgttgaagag cttaaaaagc tccttgaaca 4561 atggaaccta tagataggtt tcctattcct tacatggatt tgtcttctac aatttgccta 4621 tgccaacagg aataggtttt tgtatataat taagttaatt ttcctctggc tgttatggcc 4681 agtaacttta gcttgttttg tgcttgctgc tgtttacaga ataaattgga tcaccggtgg 4741 aattgctatc gcaatggctt gtcttgtagg cttgatgtgg ctcagctact tcattgcttc 4801 tttcagactg tttgcgcgta cgcgttccat gtggtcattc aatccagaaa ctaacattct 4861 tctcaacgtg ccactccatg gcactattct gaccagaccg cttctagaaa gtgaactcgt 4921 aatcggagct gtgatccttc gtggacatct tcgtattgct ggacaccatc taggacgctg 4981 tgacatcaag gacctgccta aagaaatcac tgttgctaca tcacgaacgc tttcttatta 5041 caaattggga gcttcgcagc gtgtagcagg agactcaggt tttgctgcat acagtcgcta 5101 caggattggc aactataaat taaacacaga ccattccagt agcagtgaca atattgcttt 5161 gcttgtacag taagtgacaa cagatgtttc atctcgttga ctttcaggtt actatagcag 5221 agatattact aattatttag aggactttta aagtttccat ttggaatctt gattacatca 5281 taaacctcat aattaaaaat ttatctaagt cactaactga gaataaatat tctcaattag 5341 atgaagagca accaatggag attgattaaa cgaacatgaa aattattctt ttcttggcac 5401 tgataacact cgctacttgt gagctttatc actaccaaga gtgtgttaga ggtacaacat 5461 agcttttaaa agaaccttgc tcttctggaa catacgaggg caattcacca tttcatcctc 5521 tagctgataa caaatttgca ctgacttgct ttagcactca atttgctttt gcttgtcctg 5581 acggcgtaaa acacgtctat cagttacgtg ccagatcagt ttcacctaaa ctgttcatca 5641 gacaagagga agttcaagaa ctttactctc caatttttct tattgttgcg gcaatagtgt 5701 ttataacact ttgcttcaca ctcaaaagaa agacagaatg attgaacttt cattaattga 5761 cttctatttg tgctttttag cctttctgct attccttgtt ttaatttagc ttattatctt 5821 ttggttctca cttgaactgc aagatcataa tgaaacttgt cacgcctaaa cgaacatgaa 5881 atttcttgtt ttcttaggaa tcatcacaac ttaggctgca tttcaccaag aatgtagttt 5941 acagtcatgt actcaacatc aaccatatgt agttgatgac ccgtgtccta ttcacttcta 6001 ttctaaatgg tatattagag taggagctag aaaatcagca cctttaattg aattgtgcgt 6061 ggatgaggct ggttctaaat cacccattca gtacatcgat atcggtaatt atacagtttc 6121 ctgtttacct tttacaatta attgccagga acctaaattg ggtagtcttg tagtgcgttg 6181 ttcgttctat gaagactttt tagagtatca tgacgttcgt gttgttttag atttcatcta 6241 aacgaacaaa ctaaaatgtc tgataatgga ccccaataac agcgaaatgc accccgcatt 6301 acgtttggtg gaccctcaga ttcaactggc agtaaccaga atggagaacg cagtggggcg 6361 cgatcaaaac aacgtcggcc ccaaggttta cccaataata ctgcgtcttg gttcaccgct 6421 ctcactcaac atggcaagga agaccttaaa ttccctcgag gacaaggcgt tccaattaac 6481 accaatagca gtccagatga ccaaattggc tactaccgaa gagctaccag acgaattcgt 6541 ggtggtgacg gtaaaatgaa agatctcagt ccaagatggt atttctacta cctaggaact 6601 gggccagaag ctggacttcc ctatggtgct aacaaagacg gcatcatatg ggttgcaact 6661 gagggagcct tgaatacacc aaaagatcac attggcaccc gcaatcctgc taacaatgct 6721 gcaatcgtgc tacaacttcc tcaaggaaca acattgccaa aaggcttcta cgcagaaggg 6781 agcagaggcg gcagtcaagc ctcttctcgt tcctcatcac gtagtcgcaa cagttcaaga 6841 aattcaactc caggcagcag taggggaact tctcctgcta gaatggctgg caatggcggt 6901 gatgctgctc ttgctttgct gctgcttgac agattgaacc agcttgagag caaaatgtct 6961 ggtaaaggcc aacaacaaca aggccaaact gtcactaaga aatctgctgc tgaggcttct 7021 aagaagcctc ggcaaaaacg tactgccact aaagcataca atgtaacaca agctttcggc 7081 agacgtggtc cagaacaaac ccaaggaaat tttggggacc aggaactaat cagacaagga 7141 actgattaca aacattggcc gcaaattgca caatttgccc ccagcgcttc agcgttcttc 7201 ggaatgtcgc gcattggcat ggaagtcaca ccttcgggaa cgtggttgac ctacacaggt 7261 gccatcaaat tggatgacaa agatccaaat ttcaaagatc aagtcatttt gctgaataag 7321 catattgacg catacaaaac attcccacca acagagccta aaaaggacaa aaagaagaag 7381 gctgatgaaa ctcaagcctt accgcagaga cagaagaaac agcaaactgt gactcttctt 7441 cctgctgcag atttggatga tttctccaaa caattgcaac aatccatgag cagtgctgac 7501 tcaactcagg cctaaactca tgcagaccac acaaggcaga tgggctatta aaacgttttc 7561 gcttttccgt ttacgatata tagtctactc ttgtgcagaa tgaattctcg taactacata 7621 gcacaagtag atgtagttaa ctttaatctc acatagcaat ctttaatcag tgtgtaacat 7681 tagggaggac ttgaaagagc caccacattt tcaccgaggc cacgcggagt acgatcgagt 7741 gtacagtgaa caatgctagg gagagctgcc tatatggaag agccctaatg tgtaaaatta 7801 attttagtag tgctatcccc atgtgatttt aatagcttct taggagaatg acaaaaacta 7861 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgcatca ctgaccattt 7921 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt 7981 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta 8041 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagtat tcaacatttc 8101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 8161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 8221 ctggatctca acagcggtaa gatccttgag agtttacgcc ccgaagaacg ttttccaatg 8281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 8341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 8401 acagaaaagc atctcacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 8461 atgagtgata acactgcggc caacttactt ctggcaacga tcggaggacc gaaggagcta 8521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 8581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 8641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 8701 gactggatgg aggcggataa agttgcagga tcacttctgc gctcggccct cccggctggc 8761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 8821 ctggggccag atggtaagcc ctcccgcatc gtagttatct acacgacggg gagtcaggca 8881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 8941 taagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca 9001 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa 9061 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac 9121 ggtgaaaacc tctgatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt 9181 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 9241 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttacccct 9301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 9361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 9421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 9481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 9541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 9601 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 9661 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9781 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg 9841 agttgattgc agtccagtta cgctggagtc aagctaatac gactcactat agacgaacaa 9901 tgtttgtttt tcttgtttta ttgccactag tctctagtca gtgtgttaat cttacaacca 9961 gaactcaatt accccctgca tacactaatt ctttcacacg tggtgtttat taccctgaca 10021 aagttttcag atcctcagtt ttacattcaa ctcaggactt gttcttacct ttcttttcca 10081 atgttacttg gttccatgct atacatgtct ctgggaccaa tggtactaag aggttttaga 10141 accctgtcct accatttaat gatggtgttt attttgcttc cactgagaag tctaacataa 10201 taagaggctg gatttttggt actactttag attcgaagac ccagtcccta cttattgtta 10261 ataacgctac taatgttgtt attaaagtct gtgaatttca attttgtaat gatccatttt 10321 tgggtgttta ttaccacaaa aacaacaaaa gttgg //