LOCUS       Exported               13805 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium
            smegmatis, expresses FnCpf1, NHEJ machinery of M. marinum.
ACCESSION   .
VERSION     .
KEYWORDS    pNHEJ-Cas12a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Yan MY, Li SS, Ding XY, Guo XP, Jin Q, Sun YC
  TITLE     A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient 
            Genome Editing in Mycobacterium tuberculosis.
  JOURNAL   mBio. 2020 Jan 28;11(1). pii: mBio.02364-19. doi: 
            10.1128/mBio.02364-19.
  PUBMED    31992616
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(123..142)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             134..949
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(207..226)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1250..1296
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(1360..1393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(1410..2033)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1941..1960
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    2348..2365
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(6381..6414)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      6480..7068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6969..6988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10282..10293
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ccacctgacg tcgggggggg gggaaagcca cgttgtgtct caaaatctct gatgttacat
       61 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      121 tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
      181 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      241 aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt ttctgaaaca
      301 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
      361 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
      421 actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc
      481 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
      541 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
      601 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
      661 acaagtctgg aaagaaatgc ataatctttt gccattctca ccggattcag tcgtcactca
      721 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
      781 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
      841 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
      901 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
      961 taattggttg taacactggc agagcattac gctgacttga cgggacggcg gctttgttga
     1021 ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac
     1081 cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc
     1141 accaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag
     1201 tcagcaacac cttcttcacg aggcagacct cactagtggc catgatggca taaaacgaaa
     1261 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagtcg atcgtacgct
     1321 agttaactac gtcgacatcg ataagcttgg cgtgaattca taacttcgta taatgtatgc
     1381 tatacgaagt tattaattgc ccatggtcat taagacccac tttcacattt aagttgtttt
     1441 tctaatccgc agatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga
     1501 tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc
     1561 ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa ggtaaaatgc
     1621 cccacagcgc tgagtgcata taacgcgttc tctagtgaaa aaccttgttg gcataaaaag
     1681 gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact
     1741 tttgctccat cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgcaaa
     1801 aaatcttgcc agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc
     1861 tcaatggcta aggcgtcgag cagggctcgc ttatttttta catgccaata caatgtaggc
     1921 tgctctacac caagtttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca
     1981 ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga cattgcgaag
     2041 tgattcctcc gatccgtggc aggagggtcg cctgcgtcgg caactcgccc aggctacacg
     2101 agagccctcg actgcgaaaa tgccgaccag gcaccaccgc actccaggct taacccggtg
     2161 cgacgatcca atattaatat tggatcgtcg gcaccgtcac ggccgtggga ggcggcacga
     2221 tccgcgacgt gatgatcggc cgcatcccca cggtgctgcg cagtgagctc tacgccatcc
     2281 cggcgttgat ctgtgcgttc gcacgcacag gcccggtgtg agaagggtct ctgacgagcg
     2341 ggagaactcc ctatcagtga tagagtttgt cctccctatc agtgatagat aggctctggg
     2401 agtacccgtg tgtacgacca gcacggcata catcatttcg acgccgagag attcgccgcc
     2461 cgaaatgagc acgatccatg tcgatctacc aagagttcgt gaataagtat agcctgagca
     2521 agaccttgcg gttcgagttg atcccgcagg gcaaaacctt ggaaaacatt aaggcgcgcg
     2581 ggctgatcct ggatgatgag aagcgggcca aggactataa aaaggccaag cagattatcg
     2641 ataaatatca tcagttcttc atcgaggaaa tcctgtcgag cgtgtgcatc agcgaagact
     2701 tgttgcagaa ctatagcgac gtgtacttca aattgaaaaa gtcggacgac gacaatctgc
     2761 aaaaagactt caagtcggcc aaagacacca tcaagaaaca gatttcggag tacatcaagg
     2821 actcggaaaa gttcaaaaac ctgttcaacc agaacttgat cgacgcgaaa aagggccagg
     2881 aaagcgattt gatcttgtgg ctgaagcagt cgaaggataa tggcattgag ttgttcaagg
     2941 ccaactcgga cattacggac attgacgagg ccctggaaat cattaaatcg tttaagggct
     3001 ggaccacgta cttcaagggc ttccatgaga accggaaaaa cgtgtattcg tcgaacgaca
     3061 ttcccacgtc gatcatctac cggatcgtcg atgacaactt gcccaagttc ttggaaaata
     3121 aagcgaaata cgagagcttg aaggacaaag cgccggaggc cattaactat gagcagatca
     3181 agaaggacct ggccgaagag ctgacctttg acattgacta caagaccagc gaggtcaatc
     3241 agcgggtctt ctcgttggat gaggtctttg aaatcgccaa tttcaacaac tatttgaacc
     3301 agagcggcat caccaaattt aataccatta tcggcgggaa gttcgtcaac ggcgagaaca
     3361 cgaagcggaa ggggatcaac gaatacatca atctgtactc gcagcagatc aacgacaaga
     3421 ccctgaaaaa gtacaagatg agcgtgctgt tcaagcaaat tctgagcgac accgagagca
     3481 agtcgttcgt cattgacaag ctggaggatg acagcgatgt ggtcaccacg atgcagtcgt
     3541 tttacgagca aatcgcggcc ttcaagaccg tggaggaaaa gagcatcaag gaaaccctga
     3601 gcctgctgtt cgatgatctg aaggcgcaga agttggatct gtcgaaaatc tacttcaaga
     3661 acgataagtc gctgaccgac ctgagccaac aggtcttcga tgattacagc gtcatcggga
     3721 ccgccgtcct ggagtacatc acccagcaga ttgcgcccaa aaacctggac aatccctcga
     3781 aaaaagagca ggagctgatt gccaagaaga ccgagaaggc gaagtacttg tcgctggaaa
     3841 ccatcaaact ggcgctggag gagttcaaca agcaccgcga cattgataaa caatgccggt
     3901 tcgaggagat tctggccaat tttgccgcca ttcccatgat cttcgatgag atcgcgcaga
     3961 ataaagacaa cctggcgcaa atctcgatca aatatcagaa tcagggcaaa aaagacctgt
     4021 tacaagcctc ggccgaagac gacgtgaaag ccatcaagga cctgttggat cagacgaaca
     4081 acttgttgca caagctgaaa attttccaca tcagccagag cgaggacaag gccaacatct
     4141 tggacaaaga cgagcacttc tacttggtgt tcgaagagtg ctacttcgaa ttggccaaca
     4201 tcgtccccct gtacaataag attcgcaatt atattacgca gaagccctat agcgacgaga
     4261 aattcaagtt gaattttgaa aattcgaccc tggcgaatgg ctgggacaag aacaaagagc
     4321 ccgacaacac cgccatcttg ttcatcaagg atgataaata ttatctgggg gtgatgaaca
     4381 aaaagaataa caagatcttt gatgacaagg ccatcaagga gaataagggc gaggggtaca
     4441 agaaaatcgt ctacaaactg ttgcccggcg ccaacaagat gttgccgaag gtcttcttca
     4501 gcgcgaagag cattaaattc tataacccca gcgaggacat cctgcgcatc cggaatcatt
     4561 cgacccacac gaagaatggc agcccgcaaa agggctacga gaagttcgag tttaacatcg
     4621 aggattgtcg gaaattcatc gacttctata aacagtcgat cagcaagcac cccgagtgga
     4681 aggatttcgg cttccgcttc agcgataccc aacgctacaa cagcatcgac gagttttatc
     4741 gggaagtgga aaaccagggg tacaagttga ccttcgagaa catttcggag tcgtacattg
     4801 actcggtcgt caaccaaggg aaactgtacc tgttccaaat ctacaataag gacttcagcg
     4861 cgtactcgaa agggcggccc aacttgcaca ccctgtattg gaaggccttg tttgacgagc
     4921 ggaacttaca agacgtcgtc tataaactga acggggaggc ggagctgttt taccgcaagc
     4981 agtcgatccc gaaaaagatt acccacccgg cgaaggaggc gatcgccaac aaaaacaagg
     5041 acaacccgaa gaaagagtcg gtcttcgaat acgatctgat caaagacaag cgctttaccg
     5101 aggacaagtt ctttttccat tgtccgatca ccatcaattt taagagcagc ggcgcgaaca
     5161 agttcaacga cgagatcaac ctgctgttga aagaaaaggc caatgatgtg cacatcctgt
     5221 cgatcgaccg gggggaacgg cacctggcct actacaccct ggtggacggc aagggcaaca
     5281 tcatcaagca ggataccttc aatatcattg ggaacgaccg gatgaaaacg aactaccacg
     5341 acaagctggc ggccatcgaa aaggatcgcg acagcgcccg caaggactgg aagaagatta
     5401 acaatatcaa ggagatgaag gaggggtact tgagccaggt cgtgcacgag atcgccaagc
     5461 tggtcattga gtacaacgcg attgtggtct ttgaagacct gaactttggc ttcaaacggg
     5521 ggcggttcaa ggtcgagaag caggtgtatc agaagctgga aaaaatgttg atcgagaagc
     5581 tgaactatct ggtgttcaaa gacaacgagt ttgacaaaac ggggggcgtc ctgcgggcct
     5641 accagttgac cgcccccttc gaaaccttca agaaaatggg caagcagacg ggcatcatct
     5701 actacgtccc ggcgggcttt acgagcaaga tctgtccggt gaccggcttc gtcaaccagc
     5761 tgtatccgaa gtatgagagc gtgtcgaaaa gccaggagtt tttctcgaag ttcgataaaa
     5821 tctgttataa cctggataag gggtacttcg aattttcgtt cgattacaaa aacttcgggg
     5881 acaaggcggc caaggggaaa tggacgatcg ccagcttcgg ctcgcgcctg attaactttc
     5941 gcaactcgga caagaaccat aactgggaca cccgggaagt gtacccgacc aaagaattgg
     6001 aaaaactgtt gaaggactat agcatcgagt acgggcacgg ggagtgcatc aaagccgcca
     6061 tctgcgggga aagcgacaag aagttttttg cgaagctgac cagcgtgctg aatacgatct
     6121 tacaaatgcg taattctaag accggcaccg agctggacta cctgatctcg ccggtggcgg
     6181 atgtcaacgg caacttcttc gacagccgcc aggccccgaa gaacatgccg caggacgcgg
     6241 atgcgaatgg cgcgtaccac atcgggctga aagggctgat gctgctgggg cggatcaaga
     6301 acaaccagga ggggaagaag ctgaatctgg tgattaagaa cgaggaatat ttcgagttcg
     6361 tccaaaatcg caacaattga ataacttcgt ataatgtatg ctatacgaag ttattaattc
     6421 tgcagagtcc tactagttcc atgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6481 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6541 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6601 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     6661 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6721 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6781 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6841 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgagggg
     6901 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6961 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7021 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7081 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7141 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     7201 cgcagccgaa cgaccgagcg caacgcgtga gcccaccagc tccgtaagtt cgggcgctgt
     7261 gtggctcgta cccgcgcatt caggcggcag ggggtctaac gggtctaagg cggcgtgtac
     7321 ggccgccaca gcggctctca gcggcccgga aacgtcctcg aaacgacgca tgtgttcctc
     7381 ctggttggta caggtggttg ggggtgctcg gctgtcgctg gtgttccacc accagggctc
     7441 gacgggagag cgggggagtg tgcagttgtg gggtggcccc tcagcgaaat atctgacttg
     7501 gagctcgtgt cggaccatac accggtgatt aatcgtggtc tactaccaag cgtgagccac
     7561 gtcgccgacg aatttgagca gctctggctg ccgtactggc cgctggcaag cgacgatctg
     7621 ctcgagggga tctaccgcca aagccgcgcg tcggccctag gccgccggta catcgaggcg
     7681 aacccaacag cgctggcaaa cctgctggtc gtggacgtag accatccaga cgcagcgctc
     7741 cgagcgctca gcgcccgggg gtcccatccg ctgcccaacg cgatcgtggg caatcgcgcc
     7801 aacggccacg cacacgcagt gtgggcactc aacgcccctg ttccacgcac cgaatacgcg
     7861 cggcgtaagc cgctcgcata catggcggcg tgcgccgaag gccttcggcg cgccgtcgat
     7921 ggcgaccgca gttactcagg cctcatgacc aaaaaccccg gccacatcgc ctgggaaacg
     7981 gaatggctcc actcagatct ctacacactc agccacatcg aggccgagct cggcgcgaac
     8041 atgccaccgc cgcgctggcg tcagcagacc acgtacaaag cggctccgac gccgctaggg
     8101 cggaattgcg cactgttcga ttccgtcagg ttgtgggcct atcgtcccgc cctcatgcgg
     8161 atctacctgc cgacccggaa cgtggacgga ctcggccgcg cgatctatgc cgagtgccac
     8221 gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc ggacagcgag
     8281 gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg catttgggcg
     8341 gacgggatcg tggtctacga ggccacactc agtgcgcgcc agtcggccat ctcgcggaag
     8401 ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc cgcgtcagcc
     8461 atggaggcat tgctatgagc gacggctaca gcgacggcta caaccggcag ccgactgtcc
     8521 gcaaaaagcg gcgcgtgacc gccgccgaag gcgctcgaat caccggacta tccgaacgcc
     8581 acgtcgtccg gctcgtggcg caggaacgca gcgagtggct cgccgagcag gctgcacgcc
     8641 gcgaacgcat ccgcgcctat cacgacgacg agggccactc ttggccgcaa acggccaaac
     8701 atttcgggct gcatctggac accgttaagc gactcggcta tcgggcgagg aaagagcgtg
     8761 cggcagaaca ggaagcggct caaaaggccc acaacgaagc cgacaatcca ccgctgttct
     8821 aacgcaattg gggagcgggt gtcgcggggg ttccgtgggg ggttccgttg caacgggtcg
     8881 gacaggtaaa agtcctggta gacgctagtt ttctggtttg ggccatgcct gtctcgttgc
     8941 gtgtttcgtt gcgcccgttt tgaataccag ccagacgaga cggggttcta cgaatcttgg
     9001 tcgataccaa gccatttccg ctgaatatcg gggagctcac cgccagaatc ggtggttgtg
     9061 gtgatgtacg tggcgaactc cgttgtagtg cctgtggtgg catccgtggc gcggccgcgg
     9121 tacccaagaa gcacagtgag tcgcggatcg atggcgggcg cggtatcggc gccgccaacg
     9181 tcacctcgtc gagagccacc acctcactcg gtgagcgcaa gacgttttca ccggcttcgc
     9241 gtaatccgtc ggcgccgcgc ttgatcaaat cgagcagggc gactcccggc gccatggcgt
     9301 agatcgaacc gtcggacaac accccgacac gctcaccgtc cgcacttcga tacgtcaccc
     9361 atttcattcg cgcaccacct cactcgggtt tttgtccggg cgcaacccac gccagctgga
     9421 ttgccgcaat cggtcatcgg gggtccactc gctatagcgc acctccccga ccagcgtcgg
     9481 cgcgacgaac gtaacaccct tggcctcgcg cgccggaagc gcagcgtcga atggggattc
     9541 gtcggtatgc agcggcgcca gcgtcttctt gaggttggcc aggtcacgtt cggtgaagcc
     9601 ggttccaaca cggccggcga agtgcaaacc gctcgggccg ggaatgccca tgagcaacga
     9661 tccaatcccg ctgctgcgcc caccctcccc aactcgccaa ccgccgatga cgacttcctg
     9721 ggtgttccag tgtttgtcct tgatccacgc cgacgagcgc cgcccgggtt gatagccgga
     9781 gtcgcggcgc ttggcaatga ctccctccca accgcgcttg tgcgagtagg ccagcgcctc
     9841 ggcgccgtca ccgggcaaca actccggaac gatcagattg cccgcactgc ccagggtttc
     9901 cagtagcttg cgccggtcgc ggtatctggc ccgcagtagc gagcgaccgt ccagaaagag
     9961 caagtcgaac gcccagaact cgatgcgggt ggcgcgcacc cggttctgca tctcgttgaa
    10021 gctgggcact ccggaggagt cgagggccac caattccccg tcgagcacca cgtggtgatc
    10081 agcgagatcg gcggccaatg aacgtaattg ggggtattcc gcggtgacgt cacgtccgct
    10141 gcgggagcgc agtcgcagcg cgccatggtc ggcttcgacc agcagccggt agccgtccca
    10201 cttgccttcg aacgcccact gacccgactc gagacctgcc accgaaccgt gggaggtgag
    10261 catgggcgcg agggtgtcga aatcgaaggc cgtctggtcc ttcatgcgat gcgccagcca
    10321 ttggtccccg ttggtctgaa tcagcgcgta ccgccccgag attcgactgc cgtgcaggtt
    10381 gacaatcacc tccccctttt ccgaaccgtt tctcgagtcg tcgcggaact tctcggtgtc
    10441 ataggtgccg gaatcccaga tgatcacctt gccggcgccg tactcccctt tcgggatgtt
    10501 gccctcgaac gtcgcgtatt ccagcggatg atcttcggtg tgcacggcca ggtggttcac
    10561 cgacgtggtt tccggcaggt ttttcggcac cgcccatgac accagtaccc catcacgctc
    10621 cagcctgaag tcgtaatgca gacggcgcgc gtgatgctct tggatcacga aggtattacc
    10681 ctgcccgaca acgggtctgg ctcggggcac cggctccggc gttttcgacg cgttgcgcat
    10741 gctgcgatat ttgagcagcc ggtcgtcgcc ggggaggtcc gcgtccagcg ccgcaatcag
    10801 atcgccatcg cgggtgaggc gggcaagcac ctcgtcgtag gtcagctggc gcagccccgg
    10861 gtcgtcgagc tcctcccagc tgcgtggtgc ggcgacggtc ggttgttcgc gcccacgcag
    10921 cgaatacggc gcgatggtgg tcttcgaacc gctgttttgg ctccagtcca ggaacacttt
    10981 cccggcccgc agactcttgg tcatggtcgc ggtgaccaac gccggcatca ccgtttccag
    11041 ttgctgcgcg acccgtttgg ccaacaccgt ggcgcccttg ctgctcaccg gctcgtcgag
    11101 cggggtgtag aggtgcaggc ccttgctacc gctggtgagc gggagggtac tcaggccgat
    11161 gtcggcgagc agatcgcgca ccgcccgcgc cacctgcgcg agttgggcca tcgtcacgcc
    11221 ctcgcccggg tccaggtcga acaccaatcg ggtggccggc ccgggcgtga ggatttcgcc
    11281 tttcgacgtc cactcggcaa cgaaccgcca ctgcgggacg tgcacctcga gggcggcctg
    11341 ttgcgcgatc caggccagcc cggtggcgct gtcgatgatc ggataggtcg tcgttcccga
    11401 ccggtgcacc acgctggcac gcggcagcca gtccggcgcc gacgacgcca gctgcttttc
    11461 gaaaaaggac gcctgatcga cgccgttggg ccagcgtttg cgagtcgccg ggcgcccggc
    11521 gatatgcggg atcatcacct cggcgatgcc ggtgtagtaa ccgaagacgt cagacttggt
    11581 ggttccggta gccgggtaca gcaccttgtc cgcgttggtc agtgtcacct gcggaccatt
    11641 cgcctgtcca ggaggccagg cctgcgccga acccatatgg ccaacgtaac ctgtcgccat
    11701 ggcacgtggg ctggtgatcg gtgaagccct gatcgacatc gtcgatgggc cggacccggc
    11761 cgagtatgtc gggggcagcc ccctcaacgt cgcggtcgga ctggccagat tgggccgcga
    11821 cgtcgatctg ctgacccaca tcggccgcga cgcccgcgga cgacggatcg ccgagtacat
    11881 tgaatcctca ggtgtacaac ttgtttcggg aagccagacg gctgatcgga cgcccaccgc
    11941 gacggccagg atcggccccg acggctcggc cacctacgcg ttcgacctgg aatggcagat
    12001 acccgacaca ccccccgtgg cgccgccgct tctcgtgcac accggttcca tcgccgccgc
    12061 gcgggaacca ggatgcttgg cggtcgcggc gctgctcgac gcctatcggg ccgcggccac
    12121 ggtgagcttc gatcccaatg tgcgtccgtc gctgagcgcc gacccggacc tgactcggga
    12181 acgcatccaa cgcctcgtcg agcgcagcga catcatcaag gccagtgccg aagacttgca
    12241 ctggatcgac cccacccagc ctccggagca gaccgcgcgg gcctggctgg cctgcggccc
    12301 ggcgattgtc gcgctgacgt tgggcgatca gggcgcagtg gcgttctgcg cggccggccc
    12361 ggcaagtgtg cccgcccagc cggttcgcgt ggtcgatacc gtcggcgccg gagacgcgtt
    12421 catggccggt ctgctcgaca cgctgtggga gcagggattg ttaggtgccg accggcgcac
    12481 cgagttgcgc aagatcggcg tgtcggcgct gacctctgca ctcgaagtcg cggccctgac
    12541 ctcggcgttg accgttgccc gtgccggcgc cgatctgccc tatcgggccg acctgcggca
    12601 gtcgcgatga ccaccgcacg cccgcaatcg ggcccggcga gggtgcacga aatgccgcgt
    12661 gatgcggcgt gtcgccgtgc cgacacgcac gctcgcgagc tgaccggggc tggggcaggc
    12721 agcaggactc cggtgccgcg gcgcactgtt aaccgacccg ggttctgggc atactgactc
    12781 catgcgctcc atctggaagg gttcaatcgc tttcgggctg gtgaatgtgc cggtcaaggt
    12841 gtacagcgcc actcaggacc acgacatcaa gttccatcag gtgcacgcca aggacaacgg
    12901 acgcatccgg tacaagcggg tatgcgaggt ctgtggcgag gtggtcgaat actccgacct
    12961 ggcccgcgcc ttcgaatccg acgatggcca gatggtgatc atcaacgacg acgacctcgc
    13021 cacgttgccc gaagatcgca gccgcgaaat cgaggtgctg gaattcgtcc cagccgaaga
    13081 agtcgatccg atgatgattg accgcagcta cttcctggag cccgactcga agtcctctaa
    13141 atcgtatgtg ctgctggcca agacgctcgc cgagacggag cggatggcga tcgtgcattt
    13201 cacgctgcgc agcaagacgc ggctggcggc gttgcgggtc aaggacttcg gcaagcgcga
    13261 ggtcatggtg gtgcataccc tgttgtggcc agacgagatc cgcgacccgg atttcccggt
    13321 gctggacaag gaggtggaga tcaaacccgc ggagctcaag atggccggcc aagtggtgga
    13381 gtcgatggca gacgacttca acccggaccg ctaccgcgac acctatcagg agcaactgca
    13441 ggagctgatc gacgccaaac tcgaaggcgg ggaagcattt accgccgaag aacaacccgc
    13501 agagctggac gagaccgaag atgtctccga cctgctggcc aagctggagg cgagcgtgaa
    13561 agcgcgttcg ggcgacggga agtcagcggc gaagaagagc accgccgaaa aggcgccggc
    13621 caagaaggcc cgggccaaac aagctgccaa gtcctgacca gcgcccgact tgttcacata
    13681 tgctcgtcgt gccgccgaga ggagtttttg cgtgaagtcc aaccgatctg gcgtcgtgct
    13741 cagcctgctg ctcgccagca cattggcgtt gtcggcatgc ggaggccatt ccagcaatac
    13801 ctcgt
//
