LOCUS Exported 7003 bp ds-DNA circular SYN 09-JAN-2024 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCAX APP 751 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7003) AUTHORS Young-Pearse TL, Bai J, Chang R, Zheng JB, LoTurco JJ, Selkoe DJ TITLE A critical function for beta-amyloid precursor protein in neuronal migration revealed by in utero RNA interference. JOURNAL J Neurosci. 2007 Dec 26. 27(52):14459-69. PUBMED 18160654 REFERENCE 2 (bases 1 to 7003) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 9, 2024 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7003 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(1..105) /gene="bla" /label=AmpR promoter enhancer 136..515 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" intron 790..1806 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1730..1752 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1814..1833 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS complement(2209..2220) /codon_start=1 /gene="cspA" /product="translation enhancing element for E. coli (Qing et al., 2004)" /label=TEE /translation="NHKV" CDS 2700..2720 /codon_start=1 /product="6xHis affinity tag" /label=7xHis /translation="HHHHHHH" primer_bind complement(4156..4175) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4221..4276 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4275..4294) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4637..4653) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4637..4653) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4650..4672) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4661..4677 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4685..4715) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4730..4751 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4810..5005 /label=SV40 promoter /note="SV40 early promoter" rep_origin 4856..4991 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4918..4937 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 5011..5145 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5048..5067) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5102..5121 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5213..5230) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5384..5972) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5464..5483) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6143..7003) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6766..6785 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 121 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt 181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag 301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc 361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg 421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc 481 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct 541 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat 601 tattttgtgc agcgatgggg gcgggggggg gggcgcgcgc caggcggggc ggggcggggc 661 gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc 721 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg 781 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg 841 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc 901 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg 961 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg 1021 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc 1081 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc 1141 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg 1201 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc 1261 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt 1321 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg 1381 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg 1441 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc 1501 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc 1561 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg 1621 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg 1681 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc 1741 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc 1801 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc 1861 aggtaccgat atcctcgagc catgctgccc ggtttggcac tgctcctgct ggccgcctgg 1921 acggctcggg cgctggaggt acccactgat ggtaatgctg gcctgctggc tgaaccccag 1981 attgccatgt tctgtggcag actgaacatg cacatgaatg tccagaatgg gaagtgggat 2041 tcagatccat cagggaccaa aacctgcatt gataccaagg aaggcatcct gcagtattgc 2101 caagaagtct accctgaact gcagatcacc aatgtggtag aagccaacca accagtgacc 2161 atccagaact ggtgcaagcg gggccgcaag cagtgcaaga cccatcccca ctttgtgatt 2221 ccctaccgct gcttagttgg tgagtttgta agtgatgccc ttctcgttcc tgacaagtgc 2281 aaattcttac accaggagag gatggatgtt tgcgaaactc atcttcactg gcacaccgtc 2341 gccaaagaga catgcagtga gaagagtacc aacttgcatg actacggcat gttgctgccc 2401 tgcggaattg acaagttccg aggggtagag tttgtgtgtt gcccactggc tgaagaaagt 2461 gacaatgtgg attctgctga tgcggaggag gatgactcgg atgtctggtg gggcggagca 2521 gacacagact atgcagatgg gagtgaagac aaagtagtag aagtagcaga ggaggaagaa 2581 gtggctgagg tggaagaaga agaagccgat gatgacgagg acgatgagga tggtgatgag 2641 gtagaggaag aggctgagga accctacgaa gaagccacag agagaaccac cagcattgcc 2701 accaccacca ccaccaccac agagtctgtg gaagaggtgg ttcgagaggt gtgctctgaa 2761 caagccgaga cggggccgtg ccgagcaatg atctcccgct ggtactttga tgtgactgaa 2821 gggaagtgtg ccccattctt ttacggcgga tgtggcggca accggaacaa ctttgacaca 2881 gaagagtact gcatggccgt gtgtggcagc gccattccta caacagcagc cagtacccct 2941 gatgccgttg acaagtatct cgagacacct ggggatgaga atgaacatgc ccatttccag 3001 aaagccaaag agaggcttga ggccaagcac cgagagagaa tgtcccaggt catgagagaa 3061 tgggaagagg cagaacgtca agcaaagaac ttgcctaaag ctgataagaa ggcagttatc 3121 cagcatttcc aggagaaagt ggaatctttg gaacaggaag cagccaacga gagacagcag 3181 ctggtggaga cacacatggc cagagtggaa gccatgctca atgaccgccg ccgcctggcc 3241 ctggagaact acatcaccgc tctgcaggct gttcctcctc ggcctcgtca cgtgttcaat 3301 atgctaaaga agtatgtccg cgcagaacag aaggacagac agcacaccct aaagcatttc 3361 gagcatgtgc gcatggtgga tcccaagaaa gccgctcaga tccggtccca ggttatgaca 3421 cacctccgtg tgatttatga gcgcatgaat cagtctctct ccctgctcta caacgtgcct 3481 gcagtggccg aggagattca ggatgaagtt gatgagctgc ttcagaaaga gcaaaactat 3541 tcagatgacg tcttggccaa catgattagt gaaccaagga tcagttacgg aaacgatgct 3601 ctcatgccat ctttgaccga aacgaaaacc accgtggagc tccttcccgt gaatggagag 3661 ttcagcctgg acgatctcca gccgtggcat tcttttgggg ctgactctgt gccagccaac 3721 acagaaaacg aagttgagcc tgttgatgcc cgccctgctg ccgaccgagg actgaccact 3781 cgaccaggtt ctgggttgac aaatatcaag acggaggaga tctctgaagt gaagatggat 3841 gcagaattcc gacatgactc aggatatgaa gttcatcatc aaaaattggt gttctttgca 3901 gaagatgtgg gttcaaacaa aggtgcaatc attggactca tggtgggcgg tgttgtcata 3961 gcgacagtga tcgtcatcac cttggtgatg ctgaagaaga aacagtacac atccattcat 4021 catggtgtgg tggaggttga cgccgctgtc accccagagg agcgccacct gtccaagatg 4081 cagcagaacg gctacgaaaa tccaacctac aagttctttg agcagatgca gaactagagc 4141 ggccgcctcg agagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct 4201 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa 4261 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa 4321 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg 4381 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca 4441 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc 4501 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg 4561 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa 4621 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 4681 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 4741 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 4801 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 4861 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 4921 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt 4981 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac 5041 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 5101 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa 5161 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 5221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 5281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 5341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 5401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 5461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 5521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 5581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 5641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 5701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 5761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 5821 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 5881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 5941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 6001 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 6061 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 6121 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 6181 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 6241 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 6301 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 6361 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 6421 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 6481 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 6541 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 6601 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 6661 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 6721 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 6781 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 6841 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 6901 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 6961 caaaaaaggg aataagggcg acacggaaat gttgaatact cat //