LOCUS Exported 10173 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector to generate hACE2 stable expressing cell line. ACCESSION . VERSION . KEYWORDS pLENTI_hACE2_PURO SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10173) TITLE De Francesco Lab Plasmids REFERENCE 2 (bases 1 to 10173) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10173 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 1..22 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 37..67 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 75..91 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 80..102 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 99..115 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 99..115 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 134..154 /label=T3 /note="T3 promoter, forward primer" promoter 136..154 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 182..408 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 409..589 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 636..761 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1254..1487 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1672..1716 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1983..2099 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2122..2425 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2426..2629 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2580..2600 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2626..2650 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind complement(5188..5208) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5193..5522 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5373..5508 /label=SV40 ori /note="SV40 origin of replication" CDS 5532..6131 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5532..5551) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6028..6048 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6198..6786 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6251..6271) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6669..6680) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6873..7106 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7178..7299 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7215..7234) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7269..7288 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7339..7474 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(7494..7513) /label=T7 /note="T7 promoter, forward primer" promoter complement(7495..7513) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7523..7540) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7523..7539) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7532..7554) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7681..8136 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7768..7787) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7978..7999 /label=F1ori-F /note="F1 origin, forward primer" promoter 8162..8266 /gene="bla" /label=AmpR promoter CDS 8267..9127 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8485..8504) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9298..9886 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9787..9806 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10040..10057 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta 1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 2101 tcgataagct tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 2161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 2221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 2281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 2341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 2401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 2461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 2521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 2581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa 2641 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact 2701 ctagaatgtc aagctcttcc tggctccttc tcagccttgt tgctgtaact gctgctcagt 2761 ccaccattga ggaacaggcc aagacatttt tggacaagtt taaccacgaa gccgaagacc 2821 tgttctatca aagttcactt gcttcttgga attataacac caatattact gaagagaatg 2881 tccaaaacat gaataatgct ggggacaaat ggtctgcctt tttaaaggaa cagtccacac 2941 ttgcccaaat gtatccacta caagaaattc agaatctcac agtcaagctt cagctgcagg 3001 ctcttcagca aaatgggtct tcagtgctct cagaagacaa gagcaaacgg ttgaacacaa 3061 ttctaaatac aatgagcacc atctacagta ctggaaaagt ttgtaaccca gataatccac 3121 aagaatgctt attacttgaa ccaggtttga atgaaataat ggcaaacagt ttagactaca 3181 atgagaggct ctgggcttgg gaaagctgga gatctgaggt cggcaagcag ctgaggccat 3241 tatatgaaga gtatgtggtc ttgaaaaatg agatggcaag agcaaatcat tatgaggact 3301 atggggatta ttggagagga gactatgaag taaatggggt agatggctat gactacagcc 3361 gcggccagtt gattgaagat gtggaacata cctttgaaga gattaaacca ttatatgaac 3421 atcttcatgc ctatgtgagg gcaaagttga tgaatgccta tccttcctat atcagtccaa 3481 ttggatgcct ccctgctcat ttgcttggtg atatgtgggg tagattttgg acaaatctgt 3541 actctttgac agttcccttt ggacagaaac caaacataga tgttactgat gcaatggtgg 3601 accaggcctg ggatgcacag agaatattca aggaggccga gaagttcttt gtatctgttg 3661 gtcttcctaa tatgactcaa ggattctggg aaaattccat gctaacggac ccaggaaatg 3721 ttcagaaagc agtctgccat cccacagctt gggacctggg gaagggcgac ttcaggatcc 3781 ttatgtgcac aaaggtgaca atggacgact tcctgacagc tcatcatgag atggggcata 3841 tccagtatga tatggcatat gctgcacaac cttttctgct aagaaatgga gctaatgaag 3901 gattccatga agctgttggg gaaatcatgt cactttctgc agccacacct aagcatttaa 3961 aatccattgg tcttctgtca cccgattttc aagaagacaa tgaaacagaa ataaacttcc 4021 tgctcaaaca agcactcacg attgttggga ctctgccatt tacttacatg ttagagaagt 4081 ggaggtggat ggtctttaaa ggggaaattc ccaaagacca gtggatgaaa aagtggtggg 4141 agatgaagcg agagatagtt ggggtggtgg aacctgtgcc ccatgatgaa acatactgtg 4201 accccgcatc tctgttccat gtttctaatg attactcatt cattcgatat tacacaagga 4261 ccctttacca attccagttt caagaagcac tttgtcaagc agctaaacat gaaggccctc 4321 tgcacaaatg tgacatctca aactctacag aagctggaca gaaactgttc aatatgctga 4381 ggcttggaaa atcagaaccc tggaccctag cattggaaaa tgttgtagga gcaaagaaca 4441 tgaatgtaag gccactgctc aactactttg agcccttatt tacctggctg aaagaccaga 4501 acaagaattc ttttgtggga tggagtaccg actggagtcc atatgcagac caaagcatca 4561 aagtgaggat aagcctaaaa tcagctcttg gagataaagc atatgaatgg aacgacaatg 4621 aaatgtacct gttccgatca tctgttgcat atgctatgag gcagtacttt ttaaaagtaa 4681 aaaatcagat gattcttttt ggggaggagg atgtgcgagt ggctaatttg aaaccaagaa 4741 tctcctttaa tttctttgtc actgcaccta aaaatgtgtc tgatatcatt cctagaactg 4801 aagttgaaaa ggccatcagg atgtcccgga gccgtatcaa tgatgctttc cgtctgaatg 4861 acaacagcct agagtttctg gggatacagc caacacttgg acctcctaac cagccccctg 4921 tttccatatg gctgattgtt tttggagttg tgatgggagt gatagtggtt ggcattgtca 4981 tcctgatctt cactgggatc agagatcgga agaagaaaaa taaagcaaga agtggagaaa 5041 atccttatgc ctccatcgat attagcaaag gagaaaataa tccaggattc caaaacactg 5101 atgatgttca gacctccttt ggtaccgaga cctcccaggt ggcgcccgct taaactagtt 5161 aacgcgtcga ccagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc 5221 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 5281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 5341 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 5401 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 5461 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 5521 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 5581 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 5641 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 5701 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 5761 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 5821 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 5881 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 5941 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 6001 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 6061 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc 6121 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc 6181 atgcatcgcc agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 6241 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 6301 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 6361 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 6421 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 6481 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 6541 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 6601 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 6661 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 6721 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 6781 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga 6841 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 6901 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6961 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7021 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7081 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7141 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 7201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 7261 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 7321 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 7381 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7441 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata 7501 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 7561 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 7621 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg 7681 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 7741 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 7801 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 7861 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 7921 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 7981 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 8041 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 8101 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 8161 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 8221 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 8281 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 8341 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 8401 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 8461 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 8521 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 8581 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 8641 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 8701 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 8761 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 8821 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 8881 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 8941 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 9001 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 9061 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 9121 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 9181 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 9241 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 9301 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 9361 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 9421 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 9481 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 9541 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 9601 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 9661 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 9721 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 9781 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 9841 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 9901 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 9961 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 10021 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 10081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 10141 cgactggaaa gcgggcagtg agcgcaacgc aat //