LOCUS Exported 9606 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation. ACCESSION . VERSION . KEYWORDS pMTTH+eGFP-bromo5(N140A) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9606) AUTHORS Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry L, Gerlich DW, Redding S, Rosen MK TITLE Organization of Chromatin by Intrinsic and Regulated Phase Separation. JOURNAL Cell. 2019 Oct 3;179(2):470-484.e21. doi: 10.1016/j.cell.2019.08.037. Epub 2019 Sep 19. PUBMED 31543265 REFERENCE 2 (bases 1 to 9606) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9606 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 85..113 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 121..137 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 126..148 /label=M13/pUC Reverse /note="In lacZ gene" CDS 207..1307 /codon_start=1 /gene="malE (mutated)" /product="maltose binding protein from E. coli" /label=MBP /note="This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol." /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF WYAVRTAVINAASGRQTVDEALKDAQT" regulatory 1164..1173 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind 1281..1304 /label=MBP-F /note="Maltose binding protein, forward primer" CDS 1356..1367 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 1380..1400 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 1410..2126 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1455..1476) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1716..1735) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2063..2084 /label=EGFP-C /note="EGFP, forward primer" CDS 4302..4322 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 4323..4340 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(4353..4370) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4353..4369) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4362..4384) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(4548..4565) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 4718..4804 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 4896..4923 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 4942..5033 /gene="bla" /label=AmpR promoter CDS 5034..5894 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5252..5271) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin complement(5936..6449) /direction=LEFT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6131..6152) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6343..6362 /label=F1ori-R /note="F1 origin, reverse primer" rep_origin 6560..7148 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7049..7068 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7302..7319 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(7425..7444) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7585..7607 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(7601..7792) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" promoter 8288..8365 /gene="lacI (mutant)" /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 8366..9448 /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind complement(8385..8404) /label=LacI-R /note="LacI, reverse primer" ORIGIN 1 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc 61 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa 121 ttgtgagcgg ataacaattt cacacaggaa acagccagtc cgtttaggtg ttttcacgag 181 cacttcacca acaaggacca tagattatga aaactgaaga aggtaaactg gtaatctgga 241 ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata 301 ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg 361 cggcaactgg cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg 421 ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc 481 cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg 541 aagcgttatc gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag 601 agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc 661 tgcaagaacc gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt 721 atgaaaacgg caagtacgac attaaagacg tgggcgtgga taacgctggc gcgaaagcgg 781 gtctgacctt cctggttgac ctgattaaaa acaaacacat gaatgcagac accgattact 841 ccatcgcaga agctgccttt aataaaggcg aaacagcgat gaccatcaac ggcccgtggg 901 catggtccaa catcgacacc agcaaagtga attatggtgt aacggtactg ccgaccttca 961 agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc aggtattaac gccgccagtc 1021 cgaacaaaga gctggcaaaa gagttcctcg aaaactatct gctgactgat gaaggtctgg 1081 aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct gaagtcttac gaggaagagt 1141 tggcgaaaga tccacgtatt gccgccacca tggaaaacgc ccagaaaggt gaaatcatgc 1201 cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg atcaacgccg 1261 ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc gcagactaat tcgagctcga 1321 acaacaacaa caataacaat aacaacaacc tcgggatcga gggaaggatt tcagaatttg 1381 aaaacctgta ttttcagggc catatggcca tggtgagcaa gggcgaggag ctgttcaccg 1441 gggtggtgcc tatcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 1501 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 1561 ccggcaagct gccagtgccg tggcctaccc tcgtgaccac cctgacctac ggcgtgcagt 1621 gcttcagccg ctacccagac cacatgaagc agcacgactt cttcaagtcc gccatgccgg 1681 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 1741 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 1801 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 1861 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 1921 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cctatcggcg 1981 acggcccagt gctgctgccg gacaaccact acctgagcac ccagtccgcc ctgagcaaag 2041 accctaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 2101 ctctcggcat ggacgagctg tacaagggtg gcagcggcgg gtcgggcggg agcggcggca 2161 gtggaggaag tgttgcctcg actaatcctc caccgccgga gaccagtaac ccaaacaagc 2221 ctaaacgtca aacgaaccaa ctccaatatc tgttgcgtgt tgttttgaaa acattgtgga 2281 aacaccaatt tgcatggcct tttcaacaac cggttgacgc ggtgaagtta aaccttccgg 2341 attattacaa aattatcaaa actccgatgg atatggggac tatcaaaaaa cggttggaga 2401 acaactacta ctggaatgcc caagagtgta ttcaagattt caacaccatg ttcacaaact 2461 gttatatcta cgcaaaacca ggagacgata tcgttctgat ggccgaggcc cttgagaaat 2521 tatttttaca gaaaatcaac gaactcccga cagaggaggg cgggtccggt ggaagtggcg 2581 gtagtggtgg gagtggtgga tcagccgcca gcacaaaccc acctccgcct gaaacgtcca 2641 acccgaacaa accgaaacgg cagactaacc agctgcagta cttacttcgg gtagtattga 2701 agacactgtg gaagcatcag ttcgcctggc cgttccaaca gccagtagat gcagttaaac 2761 ttaatttacc tgattattat aaaatcatta aaacacctat ggatatggga actattaaga 2821 aacgcctgga gaataactat tattggaatg ctcaagaatg tatccaggat ttcaatacca 2881 tgtttacgaa ctgttacatt tacgcgaaac ctggtgatga tatcgtgctt atggcagaag 2941 cactggaaaa gctgtttttg caaaaaatta atgagctgcc tactgaggaa gggggaagcg 3001 ggggctcggg ggggtcaggc ggttctggag gatctgccgc atccactaac ccgcctccgc 3061 cagagacgag taatccgaac aagccgaagc gccaaacgaa tcaattacag tatcttcttc 3121 gtgtcgtgtt aaaaacgctt tggaagcacc agtttgcgtg gccattccag cagcctgtgg 3181 atgcggttaa attaaatctc ccggactatt acaagatcat caaaacccct atggatatgg 3241 gcacgatcaa gaagcgcctc gaaaacaatt actattggaa tgcgcaagaa tgcatccagg 3301 actttaatac gatgtttact aactgctata tttatgctaa accgggcgat gatattgtat 3361 tgatggcgga agctcttgaa aaattgtttc ttcagaagat caatgaactc cctaccgaag 3421 agggaggttc gggagggagc ggtggatctg gaggctccgg agggtctgct gcgtctacca 3481 atccgccacc accggaaact agcaatccta acaaaccaaa acgtcagacc aatcaactgc 3541 aatacctctt acgcgttgtg cttaaaaccc tctggaaaca ccagtttgcc tggcctttcc 3601 agcaaccagt ggacgccgtg aagttgaacc tgccagacta ctataagatc attaagacgc 3661 caatggatat gggtacaatt aagaagcggt tggaaaacaa ctattattgg aatgcacagg 3721 aatgtatcca agacttcaat actatgttta caaattgcta catctatgcc aagccgggag 3781 atgacattgt tttaatggct gaggcattag aaaagttgtt cttacaaaag attaatgaat 3841 tgcctacgga agaaggtggc tctgggggct caggtggtag tgggggttcc gggggctccg 3901 cagcctcgac caaccctcct ccacctgaga cctctaatcc aaataaacct aagcggcaaa 3961 ccaatcagct ccagtacctg cttcgcgtag tactgaagac cttatggaaa catcaattcg 4021 cttggccgtt tcaacagcct gttgatgctg ttaagctgaa cttgcctgat tactacaaga 4081 ttatcaagac cccgatggac atggggacga ttaaaaagcg gcttgaaaat aattattatt 4141 ggaacgccca ggagtgcatt caggatttta acacgatgtt caccaattgt tatatttacg 4201 caaagccagg ggacgacatc gtactcatgg cagaggcact tgagaaactt ttcctccaga 4261 aaattaacga gttaccaacc gaggagggct cgagcggatc cgaaaacctg tattttcagg 4321 gccatcatca tcatcatcac tgaaagcttg gcactggccg tcgttttaca acgtcgtgac 4381 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 4441 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat 4501 ggcgaatggc agcttggctg ttttggcgga tgagataaga ttttcagcct gatacagatt 4561 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg 4621 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg 4681 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc 4741 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac 4801 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg 4861 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct 4921 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc 4981 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 5041 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 5101 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 5161 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 5221 gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 5281 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 5341 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 5401 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 5461 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 5521 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 5581 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 5641 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 5701 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 5761 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 5821 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 5881 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc 5941 ggttgataat cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt 6001 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 6061 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt 6121 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 6181 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag 6241 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 6301 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 6361 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 6421 cgcgcttaat gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata 6481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 6541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 6601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 6661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 6721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 6781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 6841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 6901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 6961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 7021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 7081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 7141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 7201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 7261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 7321 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 7381 gcatgtggtg cactctcagt acaccgcatg tggtgcactc tcagtacaat ctgctctgat 7441 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc 7501 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 7561 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 7621 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac 7681 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct 7741 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct 7801 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc 7861 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac 7921 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct 7981 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc 8041 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac 8101 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg 8161 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc 8221 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg 8281 aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga 8341 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta 8401 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc 8461 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt 8521 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc 8581 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc 8641 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca 8701 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga 8761 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga 8821 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga 8881 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt 8941 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc 9001 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat 9061 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg 9121 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg 9181 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga 9241 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc 9301 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc 9361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 9421 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc 9481 attaggcaca attctcatgt ttgacagctt atcatcgact gcacggtgca ccaatgcttc 9541 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata 9601 attcgt //