LOCUS Exported 9730 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive lentiviral expression of human TMPRSS4.. ACCESSION . VERSION . KEYWORDS pLEX307-TMPRSS4-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9730) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 9730) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9730 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1294..1318 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS complement(2209..2220) /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" protein_bind complement(2639..2663) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2671..2712 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2763..3351 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2816..2836) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3234..3245) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3423..3656 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3734..3855 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3771..3790) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3825..3844 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3895..4030 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(4050..4069) /label=T7 /note="T7 promoter, forward primer" promoter complement(4051..4069) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4079..4096) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4079..4095) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4088..4110) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4237..4692 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4324..4343) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4534..4555 /label=F1ori-F /note="F1 origin, forward primer" promoter 4718..4822 /gene="bla" /label=AmpR promoter CDS 4823..5683 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5041..5060) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5854..6442 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6343..6362 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6596..6613 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6730..6751 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6766..6796 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6804..6820 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6809..6831 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6828..6844 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6828..6844 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6863..6883 /label=T3 /note="T3 promoter, forward primer" promoter 6865..6883 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6911..7137 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 7138..7318 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7365..7490 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7983..8216 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8401..8445 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8676..8886 /label=SV40 promoter /note="SV40 early promoter" promoter 8698..8894 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8745..8880 /label=SV40 ori /note="SV40 origin of replication" CDS 8926..9528 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(8926..8945) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9422..9442 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9571..9688 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac 1321 aaaatgttac aggatcctga cagtgatcaa cctctgaaca gcctcgatgt caaacccctg 1381 cgcaaacccc gtatccccat ggagaccttc agaaaggtgg ggatccccat catcatagca 1441 ctactgagcc tggcgagtat catcattgtg gttgtcctca tcaaggtgat tctggataaa 1501 tactacttcc tctgcgggca gcctctccac ttcatcccga ggaagcagct gtgtgacgga 1561 gagctggact gtcccttggg ggaggacgag gagcactgtg tcaagagctt ccccgaaggg 1621 cctgcagtgg cagtccgcct ctccaaggac cgatccacac tgcaggtgct ggactcggcc 1681 acagggaact ggttctctgc ctgtttcgac aacttcacag aagctctcgc tgagacagcc 1741 tgtaggcaga tgggctacag cagcaaaccc actttcagag ctgtggagat tggcccagac 1801 caggatctgg atgttgttga aatcacagaa aacagccagg agcttcgcat gcggaactca 1861 agtgggccct gtctctcagg ctccctggtc tccctgcact gtcttgcctg tgggaagagc 1921 ctgaagaccc cccgtgtggt gggtggggag gaggcctctg tggattcttg gccttggcag 1981 gtcagcatcc agtacgacaa acagcacgtc tgtggaggga gcatcctgga cccccactgg 2041 gtcctcacgg cagcccactg cttcaggaaa cataccgatg tgttcaactg gaaggtgcgg 2101 gcaggctcag acaaactggg cagcttccca tccctggctg tggccaagat catcatcatt 2161 gaattcaacc ccatgtaccc caaagacaat gacatcgccc tcatgaagct gcagttccca 2221 ctcactttct caggcacagt caggcccatc tgtctgccct tctttgatga ggagctcact 2281 ccagccaccc cactctggat cattggatgg ggctttacga agcagaatgg agggaagatg 2341 tctgacatac tgctgcaggc gtcagtccag gtcattgaca gcacacggtg caatgcagac 2401 gatgcgtacc agggggaagt caccgagaag atgatgtgtg caggcatccc ggaagggggt 2461 gtggacacct gccagggtga cagtggtggg cccctgatgt accaatctga ccagtggcat 2521 gtggtgggca tcgttagctg gggctatggc tgcgggggcc cgagcacccc aggagtatac 2581 accaaggtct cagcctatct caactggatc tacaatgtct ggaaggctga gctgtaaaac 2641 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt 2701 ctcgattcta cgtagtaatg aactagtacc ggttaagtcg acaatcaacg cgttaagtcg 2761 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 2821 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 2881 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 2941 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 3001 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 3061 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 3121 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc 3181 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 3241 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 3301 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt 3361 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 3421 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 3481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 3541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 3601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac 3661 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 3721 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 3781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 3841 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg 3901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 4021 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac 4081 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 4141 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 4201 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4441 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 4681 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 4741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 4801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 4861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 4921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 4981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 5041 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 5101 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 5161 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 5221 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 5281 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 5341 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 5401 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 5461 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 5521 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 5581 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 5641 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 5701 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 5761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 5821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 5881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 5941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 6001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 6061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 6121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 6181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 6241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 6301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 6361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 6421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 6481 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 6541 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 6601 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 6661 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 6721 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 6781 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 6841 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct 6901 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt 6961 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg 7021 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc 7081 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg 7141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 7201 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 7261 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 7321 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga 7381 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa 7441 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc 7501 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat 7561 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg 7621 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc 7681 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc 7741 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa 7801 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat 7861 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta 7921 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga 7981 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca 8041 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat 8101 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag 8161 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg 8221 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg 8281 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa 8341 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa 8401 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac 8461 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt 8521 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca 8581 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt 8641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 8701 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 8761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 8821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 8881 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag 8941 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 9001 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 9061 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 9121 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg 9181 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 9241 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 9301 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 9361 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 9421 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 9481 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat 9541 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta 9601 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca 9661 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact 9721 ttggcgccgg //