LOCUS Exported 9436 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Constitutive lentiviral expression of human CTSB.. ACCESSION . VERSION . KEYWORDS pLEX307-CTSB-puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9436) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 9436) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9436 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1294..1318 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(2345..2369) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 2377..2418 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 2469..3057 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2522..2542) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2940..2951) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 3129..3362 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 3440..3561 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3477..3496) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3531..3550 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 3601..3736 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(3756..3775) /label=T7 /note="T7 promoter, forward primer" promoter complement(3757..3775) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3785..3802) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3785..3801) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3794..3816) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3943..4398 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4030..4049) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4240..4261 /label=F1ori-F /note="F1 origin, forward primer" promoter 4424..4528 /gene="bla" /label=AmpR promoter CDS 4529..5389 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4747..4766) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5560..6148 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6049..6068 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6302..6319 /label=L4440 /note="L4440 vector, forward primer" protein_bind 6436..6457 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6472..6502 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6510..6526 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6515..6537 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6534..6550 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6534..6550 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 6569..6589 /label=T3 /note="T3 promoter, forward primer" promoter 6571..6589 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 6617..6843 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 6844..7024 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 7071..7196 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 7689..7922 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8107..8151 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 8382..8592 /label=SV40 promoter /note="SV40 early promoter" promoter 8404..8600 /label=SV40 promoter /note="SV40 early promoter" rep_origin 8451..8586 /label=SV40 ori /note="SV40 origin of replication" CDS 8632..9234 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS" primer_bind complement(8632..8651) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 9128..9148 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 9277..9394 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac 1321 aaaatgtggc agctctgggc ctccctctgc tgcctgctgg tgttggccaa tgcccggagc 1381 aggccctctt tccatccctt aagcgatgag ctggtcaact atgtcaacaa acggaatacc 1441 acgtggcagg ccgggcacaa cttctacaac gtggacatga gctacttgaa gaggctatgt 1501 ggtaccttcc tgggtgggcc caagccaccc cagagagtta tgtttaccga ggacctgaag 1561 ctgcctgcaa gcttcgatgc acgggaacaa tggccacagt gtcccaccat caaagagatc 1621 agagaccagg gctcctgtgg cagctgctgg gccttcgggg ctgtggaagc catctctgac 1681 cggatctgca tccacaccaa tgcgcacgtc agcgtggagg tgtcggcgga ggacctgctc 1741 acctgctgtg gcagcatgtg tggggacggc tgtaatggtg gctatcctgc tgaagcttgg 1801 aacttctgga caagaaaagg cctggtttct ggtggcctct atgaatccca tgtagggtgc 1861 agaccgtact ccatccctcc ctgtgagcac cacgtcaacg gctcccggcc cccatgcacg 1921 ggggagggag atacccccaa gtgtagcaag atctgtgagc ctggctacag cccgacctac 1981 aaacaggaca agcactacgg atacaattcc tacagcgtct ccaatagcga gaaggacatc 2041 atggccgaga tctacaaaaa cggccccgtg gagggagctt tctctgtgta ttcggacttc 2101 ctgctctaca agtcaggagt gtaccaacac gtcaccggag agatgatggg tggccatgcc 2161 atccgcatcc tgggctgggg agtggagaat ggcacaccct actggctggt tgccaactcc 2221 tggaacactg actggggtga caatggcttc tttaaaatac tcagaggaca ggatcactgt 2281 ggaatcgaat cagaagtggt ggctggaatt ccacgcaccg atcagtactg ggaaaagatc 2341 taaaacccag ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc 2401 ctcggtctcg attctacgta gtaatgaact agtaccggtt aagtcgacaa tcaacgcgtt 2461 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 2521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 2581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 2641 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 2701 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 2761 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 2821 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 2881 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 2941 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 3001 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc 3061 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 3121 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 3181 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 3241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 3301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 3361 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 3421 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 3481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 3541 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 3601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 3661 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 3721 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc 3781 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 3841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 3901 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 3961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 4021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 4081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 4141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 4201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 4261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 4321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 4381 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 4441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 4501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 4561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 4621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 4681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 4741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 4801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 4861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 4921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 4981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 5041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 5101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 5161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 5221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 5281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 5341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 5401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 5521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 5581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 5641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 5701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 5761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 5821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 5881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 5941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 6001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 6061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 6121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 6181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 6241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 6301 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 6361 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 6421 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 6481 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 6541 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 6601 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg 6661 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa 6721 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga 6781 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata 6841 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 6901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 6961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 7021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 7081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 7141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 7201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 7261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 7321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 7381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 7441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 7501 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 7561 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 7621 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 7681 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 7741 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 7801 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 7861 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 7921 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 7981 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 8041 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 8101 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 8161 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 8221 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 8281 tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca 8341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 8401 gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 8461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 8521 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 8581 gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag 8641 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 8701 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 8761 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 8821 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa 8881 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 8941 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 9001 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 9061 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 9121 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 9181 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt 9241 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg 9301 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga 9361 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga 9421 tccactttgg cgccgg //