LOCUS Exported 10598 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Constitutive lentiviral expression of human FURIN.. ACCESSION . VERSION . KEYWORDS pLEX307-FURIN-blast SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10598) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 10598) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10598 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 96..1274 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 327..1265 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1222..1242 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1294..1318 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 2621..2630 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" protein_bind complement(3710..3734) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 3742..3783 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 3834..4422 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3887..3907) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4305..4316) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4494..4727 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 4805..4926 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4842..4861) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4896..4915 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 4966..5101 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(5121..5140) /label=T7 /note="T7 promoter, forward primer" promoter complement(5122..5140) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(5150..5167) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(5150..5166) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5159..5181) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 5308..5763 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5395..5414) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5605..5626 /label=F1ori-F /note="F1 origin, forward primer" promoter 5789..5893 /gene="bla" /label=AmpR promoter CDS 5894..6754 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6112..6131) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6925..7513 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7414..7433 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7667..7684 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7801..7822 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7837..7867 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7875..7891 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7880..7902 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7899..7915 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7899..7915 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 7934..7954 /label=T3 /note="T3 promoter, forward primer" promoter 7936..7954 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 7982..8208 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 8209..8389 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8436..8561 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9054..9287 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 9472..9516 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 9747..9957 /label=SV40 promoter /note="SV40 early promoter" promoter 9769..9965 /label=SV40 promoter /note="SV40 early promoter" rep_origin 9816..9951 /label=SV40 ori /note="SV40 origin of replication" regulatory 9987..9996 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9993..10391 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" misc_feature 10439..10556 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct 61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag 121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg 181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac 1321 aaaatggagc tgaggccctg gttgctatgg gtggtagcag caacaggaac cttggtcctg 1381 ctagcagctg atgctcaggg ccagaaggtc ttcaccaaca cgtgggctgt gcgcatccct 1441 ggaggcccag cggtggccaa cagtgtggca cggaagcatg ggttcctcaa cctgggccag 1501 atcttcgggg actattacca cttctggcat cgaggagtga cgaagcggtc cctgtcgcct 1561 caccgcccgc ggcacagccg gctgcagagg gagcctcaag tacagtggct ggaacagcag 1621 gtggcaaagc gacggactaa acgggacgtg taccaggagc ccacagaccc caagtttcct 1681 cagcagtggt acctgtctgg tgtcactcag cgggacctga atgtgaaggc ggcctgggcg 1741 cagggctaca cagggcacgg cattgtggtc tccattctgg acgatggcat cgagaagaac 1801 cacccggact tggcaggcaa ttatgatcct ggggccagtt ttgatgtcaa tgaccaggac 1861 cctgaccccc agcctcggta cacacagatg aatgacaaca ggcacggcac acggtgtgcg 1921 ggggaagtgg ctgcggtggc caacaacggt gtctgtggtg taggtgtggc ctacaacgcc 1981 cgcattggag gggtgcgcat gctggatggc gaggtgacag atgcagtgga ggcacgctcg 2041 ctgggcctga accccaacca catccacatc tacagtgcca gctggggccc cgaggatgac 2101 ggcaagacag tggatgggcc agcccgcctc gccgaggagg ccttcttccg tggggttagc 2161 cagggccgag gggggctggg ctccatcttt gtctgggcct cggggaacgg gggccgggaa 2221 catgacagct gcaactgcga cggctacacc aacagtatct acacgctgtc catcagcagc 2281 gccacgcagt ttggcaacgt gccgtggtac agcgaggcct gctcgtccac actggccacg 2341 acctacagca gtggcaacca gaatgagaag cagatcgtga cgactgactt gcggcagaag 2401 tgcacggagt ctcacacggg cacctcagcc tctgccccct tagcagccgg catcattgct 2461 ctcaccctgg aggccaataa gaacctcaca tggcgggaca tgcaacacct ggtggtacag 2521 acctcgaagc cagcccacct caatgccaac gactgggcca ccaatggtgt gggccggaaa 2581 gtgagccact catatggcta cgggcttttg gacgcaggcg ccatggtggc cctggcccag 2641 aattggacca cagtggcccc ccagcggaag tgcatcatcg acatcctcac cgagcccaaa 2701 gacatcggga aacggctcga ggtgcggaag accgtgaccg cgtgcctggg cgagcccaac 2761 cacatcactc ggctggagca cgctcaggcg cggctcaccc tgtcctataa tcgccgtggc 2821 gacctggcca tccacctggt cagccccatg ggcacccgct ccaccctgct ggcagccagg 2881 ccacatgact actccgcaga tgggtttaat gactgggcct tcatgacaac tcattcctgg 2941 gatgaggatc cctctggcga gtgggtccta gagattgaaa acaccagcga agccaacaac 3001 tatgggacgc tgaccaagtt caccctcgta ctctatggca ccgcccctga ggggctgccc 3061 gtacctccag aaagcagtgg ctgcaagacc ctcacgtcca gtcaggcctg tgtggtgtgc 3121 gaggaaggct tctccctgca ccagaagagc tgtgtccagc actgccctcc aggcttcgcc 3181 ccccaagtcc tcgatacgca ctatagcacc gagaatgacg tggagaccat ccgggccagc 3241 gtctgcgccc cctgccacgc ctcatgtgcc acatgccagg ggccggccct gacagattgc 3301 ctcagctgcc ccagccacgc ctccttggac cctgtggagc agacttgctc ccggcaaagc 3361 cagagcagcc gagagtcccc gccacagcag cagccacctc ggctgccccc ggaggtggag 3421 gcggggcaac ggctgcgggc agggctgctg ccctcacacc tgcctgaggt ggtggccggc 3481 ctcagctgcg ccttcatcgt gctggtcttc gtcactgtct tcctggtcct gcagctgcgc 3541 tctggcttta gttttcgggg ggtgaaggtg tacaccatgg accgtggcct catctcctac 3601 aaggggctgc cccctgaagc ctggcaggag gagtgcccgt ctgactcaga agaggacgag 3661 ggccggggcg agaggaccgc ctttatcaaa gaccagagcg ccctctaaaa cccagctttc 3721 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct 3781 acgtagtaat gaactagtac cggttaagtc gacaatcaac gcgttaagtc gacaatcaac 3841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta 3901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 3961 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg 4021 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg 4081 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca 4141 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca 4201 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 4261 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag 4321 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc 4381 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat 4441 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 4501 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt 4561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 4621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 4681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag 4741 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 4801 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 4921 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact 4981 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 5041 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 5101 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 5161 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 5221 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 5281 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 5341 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5401 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5461 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5521 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 5581 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 5641 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 5701 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 5761 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 5821 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 5881 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 5941 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 6001 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 6061 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 6121 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 6181 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 6241 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 6301 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 6361 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 6421 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 6481 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 6541 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 6601 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 6661 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 6721 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 6781 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 6841 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 6901 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 6961 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 7021 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 7081 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 7141 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 7201 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 7261 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 7321 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 7381 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 7441 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 7501 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 7561 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 7621 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 7681 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 7741 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 7801 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 7861 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 7921 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 7981 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 8041 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 8101 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 8161 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 8221 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 8281 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 8341 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 8401 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 8461 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 8521 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 8581 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 8641 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 8701 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 8761 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 8821 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 8881 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 8941 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 9001 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 9061 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 9121 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 9181 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 9241 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 9301 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 9361 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 9421 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 9481 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 9541 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 9601 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 9661 cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca 9721 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt 9781 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 9841 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 9901 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 9961 gcaaaaagct ttctagaggt accgtggcca ccatggccaa gcctttgtct caagaagaat 10021 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca 10081 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc 10141 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag 10201 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct 10261 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg 10321 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt 10381 gggagggcta agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt 10441 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag 10501 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg 10561 tttattacag ggacagcaga gatccacttt ggcgccgg //