LOCUS Exported 3552 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Cloning compatible entry vector for the human CTSL gene.. ACCESSION . VERSION . KEYWORDS pDONR221-CTSL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3552) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 3552) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3552 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1677..1776) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1793..1812) /label=T7 /note="T7 promoter, forward primer" promoter complement(1794..1812) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1935..1954) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 1946..2755 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2013..2032) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 2902..3490 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3391..3410 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctac aaaatgaatc ctacactcat ccttgctgcc ttttgcctgg gaattgcctc 721 agctactcta acatttgatc acagtttaga ggcacagtgg accaagtgga aggcgatgca 781 caacagatta tacggcatga atgaagaagg atggaggaga gcagtgtggg agaagaacgt 841 gaagatgatt gaactgcaca atcaggaata cagggaaggg aaacacagct tcacaatggc 901 catgaacgcc tttggagaca tgaccagtga agaattcagg caggtgatga atggctttca 961 aaaccgtaag cccaggaagg ggaaagtgtt ccaggaacct ctgttttatg aggcccccag 1021 atctgtggat tggagagaga aaggctacgt gactcctgtg aagaatcagg gtcagtgtgg 1081 ttcttgttgg gcttttagtg ctactggtgc tcttgaagga cagatgttcc ggaaaactgg 1141 gaggcttatc tcactgagtg agcagaatct ggtagactgc tctgggcctc aaggcaatga 1201 aggctgcaat ggtggcctaa tggattatgc tttccagtat gttcaggata atggaggcct 1261 ggactctgag gaatcctatc catatgaggc aacagaagaa tcctgtaagt acaatcccaa 1321 gtattctgtt gctaatgaca ccggctttgt ggacatccct aagcaggaga aggccctgat 1381 gaaggcagtt gcaactgtgg ggcccatttc tgttgctatt gatgcaggtc atgagtcctt 1441 cctgttctat aaagaaggca tttattttga gccagactgt agcagtgaag acatggatca 1501 tggtgtgctg gtggttggct acggatttga aagcacagaa tcagataaca ataaatattg 1561 gctggtgaag aacagctggg gtgaagaatg gggcatgggt ggctacgtaa agatggccaa 1621 agaccggaga aaccattgtg gaattgcctc agcagccagc taccccactg tgtagaaccc 1681 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga 1741 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag 1801 tgagtcgtat tacatggtca tagctgtttc ttggcagctc tggcccgtgt ctcaaaatct 1861 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta 1921 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg 1981 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg 2041 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct 2101 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg 2161 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc 2221 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc 2281 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat 2341 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc 2401 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc 2461 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt 2521 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg 2581 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa 2641 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga 2701 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga 2761 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag 2821 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt 2881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 2941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 3001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta 3061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 3121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 3181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 3241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 3301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 3361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 3421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 3481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 3541 tgctcacatg tt //