LOCUS Exported 3864 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Cloning compatible entry vector for the human TMPRSS4 gene.. ACCESSION . VERSION . KEYWORDS pDONR221-TMPRSS4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3864) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 3864) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3864 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(1559..1570) /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" protein_bind complement(1989..2088) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2105..2124) /label=T7 /note="T7 promoter, forward primer" promoter complement(2106..2124) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2247..2266) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2258..3067 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2325..2344) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3214..3802 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3703..3722 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctac aaaatgttac aggatcctga cagtgatcaa cctctgaaca gcctcgatgt 721 caaacccctg cgcaaacccc gtatccccat ggagaccttc agaaaggtgg ggatccccat 781 catcatagca ctactgagcc tggcgagtat catcattgtg gttgtcctca tcaaggtgat 841 tctggataaa tactacttcc tctgcgggca gcctctccac ttcatcccga ggaagcagct 901 gtgtgacgga gagctggact gtcccttggg ggaggacgag gagcactgtg tcaagagctt 961 ccccgaaggg cctgcagtgg cagtccgcct ctccaaggac cgatccacac tgcaggtgct 1021 ggactcggcc acagggaact ggttctctgc ctgtttcgac aacttcacag aagctctcgc 1081 tgagacagcc tgtaggcaga tgggctacag cagcaaaccc actttcagag ctgtggagat 1141 tggcccagac caggatctgg atgttgttga aatcacagaa aacagccagg agcttcgcat 1201 gcggaactca agtgggccct gtctctcagg ctccctggtc tccctgcact gtcttgcctg 1261 tgggaagagc ctgaagaccc cccgtgtggt gggtggggag gaggcctctg tggattcttg 1321 gccttggcag gtcagcatcc agtacgacaa acagcacgtc tgtggaggga gcatcctgga 1381 cccccactgg gtcctcacgg cagcccactg cttcaggaaa cataccgatg tgttcaactg 1441 gaaggtgcgg gcaggctcag acaaactggg cagcttccca tccctggctg tggccaagat 1501 catcatcatt gaattcaacc ccatgtaccc caaagacaat gacatcgccc tcatgaagct 1561 gcagttccca ctcactttct caggcacagt caggcccatc tgtctgccct tctttgatga 1621 ggagctcact ccagccaccc cactctggat cattggatgg ggctttacga agcagaatgg 1681 agggaagatg tctgacatac tgctgcaggc gtcagtccag gtcattgaca gcacacggtg 1741 caatgcagac gatgcgtacc agggggaagt caccgagaag atgatgtgtg caggcatccc 1801 ggaagggggt gtggacacct gccagggtga cagtggtggg cccctgatgt accaatctga 1861 ccagtggcat gtggtgggca tcgttagctg gggctatggc tgcgggggcc cgagcacccc 1921 aggagtatac accaaggtct cagcctatct caactggatc tacaatgtct ggaaggctga 1981 gctgtaaaac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat 2041 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga 2101 tatcccctat agtgagtcgt attacatggt catagctgtt tcttggcagc tctggcccgt 2161 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa 2221 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac 2281 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 2341 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc 2401 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 2461 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 2521 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt 2581 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 2641 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 2701 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 2761 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc 2821 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 2881 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 2941 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 3001 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 3061 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 3121 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc 3181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 3241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 3301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 3361 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3601 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 3781 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 3841 ttgctggcct tttgctcaca tgtt //