LOCUS Exported 4029 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Cloning compatible entry vector for the human TMPRSS2 gene.. ACCESSION . VERSION . KEYWORDS pDONR221-TMPRSS2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4029) AUTHORS Chavez Lab TITLE Coronavirus host receptors and proteases JOURNAL Unpublished REFERENCE 2 (bases 1 to 4029) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4029 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2154..2253) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2270..2289) /label=T7 /note="T7 promoter, forward primer" promoter complement(2271..2289) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2412..2431) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2423..3232 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2490..2509) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3379..3967 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3868..3887 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctac aaaatggctt tgaactcagg gtcaccacca gctattggac cttactatga 721 aaaccatgga taccaaccgg aaaaccccta tcccgcacag cccactgtgg tccccactgt 781 ctacgaggtg catccggctc agtactaccc gtcccccgtg ccccagtacg ccccgagggt 841 cctgacgcag gcttccaacc ccgtcgtctg cacgcagccc aaatccccat ccgggacagt 901 gtgcacctca aagactaaga aagcactgtg catcaccttg accctgggga ccttcctcgt 961 gggagctgcg ctggccgctg gcctactctg gaagttcatg ggcagcaagt gctccaactc 1021 tgggatagag tgcgactcct caggtacctg catcaacccc tctaactggt gtgatggcgt 1081 gtcacactgc cccggcgggg aggacgagaa tcggtgtgtt cgcctctacg gaccaaactt 1141 catccttcag gtgtactcat ctcagaggaa gtcctggcac cctgtgtgcc aagacgactg 1201 gaacgagaac tacgggcggg cggcctgcag ggacatgggc tataagaata atttttactc 1261 tagccaagga atagtggatg acagcggatc caccagcttt atgaaactga acacaagtgc 1321 cggcaatgtc gatatctata aaaaactgta ccacagtgat gcctgttctt caaaagcagt 1381 ggtttcttta cgctgtatag cctgcggggt caacttgaac tcaagccgcc agagcaggat 1441 cgtgggcggc gagagcgcgc tcccgggggc ctggccctgg caggtcagcc tgcacgtcca 1501 gaacgtccac gtgtgcggag gctccatcat cacccccgag tggatcgtga cagccgccca 1561 ctgcgtggaa aaacctctta acaatccatg gcattggacg gcatttgcgg ggattttgag 1621 acaatctttc atgttctatg gagccggata ccaagtagaa aaagtgattt ctcatccaaa 1681 ttatgactcc aagaccaaga acaatgacat tgcgctgatg aagctgcaga agcctctgac 1741 tttcaacgac ctagtgaaac cagtgtgtct gcccaaccca ggcatgatgc tgcagccaga 1801 acagctctgc tggatttccg ggtggggggc caccgaggag aaagggaaga cctcagaagt 1861 gctgaacgct gccaaggtgc ttctcattga gacacagaga tgcaacagca gatatgtcta 1921 tgacaacctg atcacaccag ccatgatctg tgccggcttc ctgcagggga acgtcgattc 1981 ttgccagggt gacagtggag ggcctctggt cacttcgaag aacaatatct ggtggctgat 2041 aggggataca agctggggtt ctggctgtgc caaagcttac agaccaggag tgtacgggaa 2101 tgtgatggta ttcacggact ggatttatcg acaaatgagg gcagacggct aaaacccagc 2161 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca 2221 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga 2281 gtcgtattac atggtcatag ctgtttcttg gcagctctgg cccgtgtctc aaaatctctg 2341 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 2401 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 2461 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 2521 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 2581 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 2641 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 2701 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 2761 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 2821 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 2881 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 2941 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 3001 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 3061 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 3121 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 3181 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 3241 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc 3301 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga 3361 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 3421 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 3481 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 3541 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 3601 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 3661 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 3721 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 3781 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 3841 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 3901 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 3961 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 4021 tcacatgtt //