LOCUS       Exported               11196 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Lentiviral vector expressing human codon-optimized _C-terminal 
            Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- 
            and C-terminal NLS and Neomycin/G418/Geneticin resistance from 
            EF-1alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    plenti-Lb-Cas12a-2xNLS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, 
            Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, 
            Myers CL, Blencowe BJ, Moffat J
  TITLE     Genetic interaction mapping and exon-resolution functional genomics 
            with a hybrid Cas9-Cas12a platform.
  JOURNAL   Nat Biotechnol. 2020 May;38(5):638-648. doi: 
            10.1038/s41587-020-0437-z. Epub 2020 Mar 16.
  PUBMED    32249828
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1486..1506
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    1707..1824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1893..2104
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2133..2153
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2178..5858
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5859..5906
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5913..5942
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5952..6005
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6009..6803
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6063..6082)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6673..6692
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6822..7410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6875..6895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7293..7304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7482..7715
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7793..7914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7830..7849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7884..7903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7954..8089
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8016..8035
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8109..8128)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8110..8128)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8138..8155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8138..8154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8147..8169)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8296..8751
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8383..8402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8593..8614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8777..8881
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8882..9742
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9100..9119)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9913..10501
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10402..10421
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10655..10672
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10789..10810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10825..10855
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10863..10879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10868..10890
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10887..10903
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10887..10903
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10922..10942
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10924..10942
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10970..11196
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccagagaggg cctatttccc
     1501 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
     1561 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
     1621 gggtagtttg cagttttaaa attatgtttt aaaatggact atcataaatt ctagatcttg
     1681 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1741 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1801 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     1861 cgccggctcg agtggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     1921 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     1981 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2041 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2101 acaggtgtcg tgacgcggtt aacaccatgg ccccaaagaa gaagcggaag gtcggtatcc
     2161 acggagtccc agcagccagc aagctggaga agtttacaaa ctgctactcc ctgtctaaga
     2221 ccctgaggtt caaggccatc cctgtgggca agacccagga gaacatcgac aataagcggc
     2281 tgctggtgga ggacgagaag agagccgagg attataaggg cgtgaagaag ctgctggatc
     2341 gctactatct gtcttttatc aacgacgtgc tgcacagcat caagctgaag aatctgaaca
     2401 attacatcag cctgttccgg aagaaaacca gaaccgagaa ggagaataag gagctggaga
     2461 acctggagat caatctgcgg aaggagatcg ccaaggcctt caagggcaac gagggctaca
     2521 agtccctgtt taagaaggat atcatcgaga caatcctgcc agagttcctg gacgataagg
     2581 acgagatcgc cctggtgaac agcttcaatg gctttaccac agccttcacc ggcttctttg
     2641 ataacagaga gaatatgttt tccgaggagg ccaagagcac atccatcgcc ttcaggtgta
     2701 tcaacgagaa tctgacccgc tacatctcta atatggacat cttcgagaag gtggacgcca
     2761 tctttgataa gcacgaggtg caggagatca aggagaagat cctgaacagc gactatgatg
     2821 tggaggattt ctttgagggc gagttcttta actttgtgct gacacaggag ggcatcgacg
     2881 tgtataacgc catcatcggc ggcttcgtga ccgagagcgg cgagaagatc aagggcctga
     2941 acgagtacat caacctgtat aatcagaaaa ccaagcagaa gctgcctaag tttaagccac
     3001 tgtataagca ggtgctgagc gatcgggagt ctctgagctt ctacggcgag ggctatacat
     3061 ccgatgagga ggtgctggag gtgtttagaa acaccctgaa caagaacagc gagatcttca
     3121 gctccatcaa gaagctggag aagctgttca agaattttga cgagtactct agcgccggca
     3181 tctttgtgaa gaacggcccc gccatcagca caatctccaa ggatatcttc ggcgagtgga
     3241 acgtgatccg ggacaagtgg aatgccgagt atgacgatat ccacctgaag aagaaggccg
     3301 tggtgaccga gaagtacgag gacgatcgga gaaagtcctt caagaagatc ggctcctttt
     3361 ctctggagca gctgcaggag tacgccgacg ccgatctgtc tgtggtggag aagctgaagg
     3421 agatcatcat ccagaaggtg gatgagatct acaaggtgta tggctcctct gagaagctgt
     3481 tcgacgccga ttttgtgctg gagaagagcc tgaagaagaa cgacgccgtg gtggccatca
     3541 tgaaggacct gctggattct gtgaagagct tcgagaatta catcaaggcc ttctttggcg
     3601 agggcaagga gacaaacagg gacgagtcct tctatggcga ttttgtgctg gcctacgaca
     3661 tcctgctgaa ggtggaccac atctacgatg ccatccgcaa ttatgtgacc cagaagccct
     3721 actctaagga taagttcaag ctgtattttc agaaccctca gttcatgggc ggctgggaca
     3781 aggataagga gacagactat cgggccacca tcctgagata cggctccaag tactatctgg
     3841 ccatcatgga taagaagtac gccaagtgcc tgcagaagat cgacaaggac gatgtgaacg
     3901 gcaattacga gaagatcaac tataagctgc tgcccggccc taataagatg ctgccaaagg
     3961 tgttcttttc taagaagtgg atggcctact ataaccccag cgaggacatc cagaagatct
     4021 acaagaatgg cacattcaag aagggcgata tgtttaacct gaatgactgt cacaagctga
     4081 tcgacttctt taaggatagc atctcccggt atccaaagtg gtccaatgcc tacgatttca
     4141 acttttctga gacagagaag tataaggaca tcgccggctt ttacagagag gtggaggagc
     4201 agggctataa ggtgagcttc gagtctgcca gcaagaagga ggtggataag ctggtggagg
     4261 agggcaagct gtatatgttc cagatctata acaaggactt ttccgataag tctcacggca
     4321 cacccaatct gcacaccatg tacttcaagc tgctgtttga cgagaacaat cacggacaga
     4381 tcaggctgag cggaggagca gagctgttca tgaggcgcgc ctccctgaag aaggaggagc
     4441 tggtggtgca cccagccaac tcccctatcg ccaacaagaa tccagataat cccaagaaaa
     4501 ccacaaccct gtcctacgac gtgtataagg ataagaggtt ttctgaggac cagtacgagc
     4561 tgcacatccc aatcgccatc aataagtgcc ccaagaacat cttcaagatc aatacagagg
     4621 tgcgcgtgct gctgaagcac gacgataacc cctatgtgat cggcatcgat aggggcgagc
     4681 gcaatctgct gtatatcgtg gtggtggacg gcaagggcaa catcgtggag cagtattccc
     4741 tgaacgagat catcaacaac ttcaacggca tcaggatcaa gacagattac cactctctgc
     4801 tggacaagaa ggagaaggag aggttcgagg cccgccagaa ctggacctcc atcgagaata
     4861 tcaaggagct gaaggccggc tatatctctc aggtggtgca caagatctgc gagctggtgg
     4921 agaagtacga tgccgtgatc gccctggagg acctgaactc tggctttaag aatagccgcg
     4981 tgaaggtgga gaagcaggtg tatcagaagt tcgagaagat gctgatcgat aagctgaact
     5041 acatggtgga caagaagtct aatccttgtg caacaggcgg cgccctgaag ggctatcaga
     5101 tcaccaataa gttcgagagc tttaagtcca tgtctaccca gaacggcttc atcttttaca
     5161 tccctgcctg gctgacatcc aagatcgatc catctaccgg ctttgtgaac ctgctgaaaa
     5221 ccaagtatac cagcatcgcc gattccaaga agttcatcag ctcctttgac aggatcatgt
     5281 acgtgcccga ggaggatctg ttcgagtttg ccctggacta taagaacttc tctcgcacag
     5341 acgccgatta catcaagaag tggaagctgt actcctacgg caaccggatc agaatcttcc
     5401 ggaatcctaa gaagaacaac gtgttcgact gggaggaggt gtgcctgacc agcgcctata
     5461 aggagctgtt caacaagtac ggcatcaatt atcagcaggg cgatatcaga gccctgctgt
     5521 gcgagcagtc cgacaaggcc ttctactcta gctttatggc cctgatgagc ctgatgctgc
     5581 agatgcggaa cagcatcaca ggccgcaccg acgtggattt tctgatcagc cctgtgaaga
     5641 actccgacgg catcttctac gatagccgga actatgaggc ccaggagaat gccatcctgc
     5701 caaagaacgc cgacgccaat ggcgcctata acatcgccag aaaggtgctg tgggccatcg
     5761 gccagttcaa gaaggccgag gacgagaagc tggataaggt gaagatcgcc atctctaaca
     5821 aggagtggct ggagtacgcc cagaccagcg tgaagcacaa aaggccggcg gccacgaaaa
     5881 aggccggcca ggcaaaaaag aaaaagggat ccgaacaaaa actcatctca gaagaggatc
     5941 tgggcagtgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     6001 gcccaaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6061 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6121 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6181 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6241 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6301 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6361 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6421 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6481 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6541 tgcccgacgg cgatgatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6601 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6661 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6721 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6781 gccttcttga cgagttcttc tgataaacgc gttaagtcga caatcaacct ctggattaca
     6841 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6901 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     6961 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7021 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7081 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7141 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7201 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     7261 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7321 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7381 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     7441 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7501 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7561 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7621 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7681 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     7741 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7801 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7861 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7921 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7981 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8041 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     8101 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     8161 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8221 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8281 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8341 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8401 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8461 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8521 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8581 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8641 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     8701 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     8761 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8821 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8881 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8941 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9001 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9061 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9121 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9181 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9241 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9301 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9361 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9421 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9481 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9541 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9601 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9661 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9721 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9781 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9841 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10561 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10621 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10681 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10741 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    10801 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    10861 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
    10921 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
    10981 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
    11041 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
    11101 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
    11161 gatattgtat ttaagtgcct agctcgatac ataaac
//
