LOCUS Exported 10039 bp ds-DNA circular SYN 20-AUG-2021 DEFINITION CRISPaint frame selector +2. ACCESSION . VERSION . KEYWORDS pCAS9-mCherry-Frame +2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10039) AUTHORS Schmid-Burgk JL, Honing K, Ebert TS, Hornung V TITLE CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. JOURNAL Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. PUBMED 27465542 REFERENCE 2 (bases 1 to 10039) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10039 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" CDS 946..1362 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1292..1314 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1330..1346 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" promoter 1373..1613 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 1373..1393 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 1544..1563 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 1642..1717 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 1758..2137 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2138..2341 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2292..2312 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 2402..2411 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2411..2434 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 2435..3139 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE GRHSTGGMDELYK" primer_bind complement(2577..2595) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2845..2864 /label=mCherry-F /note="mCherry, forward primer" CDS 3164..3217 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3218..7321 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 7334..7354 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" LTR 7533..8002 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(8132..8149) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8303..8891) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8383..8402) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9051..9911) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9674..9693 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9912..10016) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ctagttagct aactagctct gtatctggcg gacccgtggt 661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg 721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga 781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg 841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg 901 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc 961 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag 1021 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt 1081 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc 1141 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg 1201 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg 1261 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct 1321 cgatcctccc tttatccagc cctcactcct tctctaggcg ccggccggat ccgagggcct 1381 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg 1441 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata 1501 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac 1561 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac 1621 cgggccagta cccaaaaagc ggttttagag ctagaaatag caagttaaaa taaggctagt 1681 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttcgaatt ccgatgtacg 1741 ggccagatat acgcgttgac attgattatt gtctagttat taatagtaat caattacggg 1801 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 1861 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 1921 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 1981 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 2041 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 2101 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat 2161 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 2221 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc 2281 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 2341 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaagc ttccgcgtac 2401 ggccaccatg gattataagg atgacgacga taaggtgagc aagggcgagg aggataacat 2461 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 2521 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 2581 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 2641 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 2701 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 2761 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 2821 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 2881 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 2941 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 3001 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 3061 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 3121 catggacgag ctgtacaagg tcgctaccat cgagggatcc ggagagggca gaggaagtct 3181 gctaacatgc ggtgacgtcg aggagaatcc tggcccaatg gacaagaagt acagcatcgg 3241 cctggacatc ggcaccaaca gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc 3301 cagcaagaag ttcaaggtgc tgggcaacac cgaccgccac agcatcaaga agaacctgat 3361 cggcgccctg ctgttcgaca gcggcgagac cgccgaggcc acccgcctga agcgcaccgc 3421 ccgccgccgc tacacccgcc gcaagaaccg catctgctac ctgcaggaga tcttcagcaa 3481 cgagatggcc aaggtggacg acagcttctt ccaccgcctg gaggagagct tcctggtgga 3541 ggaggacaag aagcacgagc gccaccccat cttcggcaac atcgtggacg aggtggccta 3601 ccacgagaag taccccacca tctaccacct gcgcaagaag ctggtggaca gcaccgacaa 3661 ggccgacctg cgcctgatct acctggccct ggcccacatg atcaagttcc gcggccactt 3721 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct 3781 ggtgcagacc tacaaccagc tgttcgagga gaaccccatc aacgccagcg gcgtggacgc 3841 caaggccatc ctgagcgccc gcctgagcaa gagccgccgc ctggagaacc tgatcgccca 3901 gctgcccggc gagaagaaga acggcctgtt cggcaacctg atcgccctga gcctgggcct 3961 gacccccaac ttcaagagca acttcgacct ggccgaggac gccaagctgc agctgagcaa 4021 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga 4081 cctgttcctg gccgccaaga acctgagcga cgccatcctg ctgagcgaca tcctgcgcgt 4141 gaacaccgag atcaccaagg cccccctgag cgccagcatg atcaagcgct acgacgagca 4201 ccaccaggac ctgaccctgc tgaaggccct ggtgcgccag cagctgcccg agaagtacaa 4261 ggagatcttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgacg gcggcgccag 4321 ccaggaggag ttctacaagt tcatcaagcc catcctggag aagatggacg gcaccgagga 4381 gctgctggtg aagctgaacc gcgaggacct gctgcgcaag cagcgcacct tcgacaacgg 4441 cagcatcccc caccagatcc acctgggcga gctgcacgcc atcctgcgcc gccaggagga 4501 cttctacccc ttcctgaagg acaaccgcga gaagatcgag aagatcctga ccttccgcat 4561 cccctactac gtcggccccc tggcccgcgg caacagccgc ttcgcctgga tgacccgcaa 4621 gagcgaggag accatcaccc cctggaactt cgaggaggtg gtggacaagg gcgccagcgc 4681 ccagagcttc atcgagcgca tgaccaactt cgacaagaac ctgcccaacg agaaggtgct 4741 gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga ccaaggtgaa 4801 gtacgtgacc gagggcatgc gcaagcccgc cttcctgagc ggcgagcaga agaaggccat 4861 cgtggacctg ctgttcaaga ccaaccgcaa ggtgaccgtg aagcagctga aggaggacta 4921 cttcaagaag atcgagtgct tcgacagcgt ggagatcagc ggcgtggagg accgcttcaa 4981 cgccagcctg ggcacctacc acgacctgct gaagatcatc aaggacaagg acttcctgga 5041 caacgaggag aacgaggaca tcctggagga catcgtgctg accctgaccc tgttcgagga 5101 ccgcgagatg atcgaggagc gcctgaagac ctacgcccac ctgttcgacg acaaggtgat 5161 gaagcagctg aagcgccgcc gctacaccgg ctggggccgc ctgagccgca agctgatcaa 5221 cggcatccgc gacaagcaga gcggcaagac catcctggac ttcctgaaga gcgacggctt 5281 cgccaaccgc aacttcatgc agctgatcca cgacgacagc ctgaccttca aggaggacat 5341 ccagaaggcc caggtgagcg gccagggcga cagcctgcac gagcacatcg ccaacctggc 5401 cggcagcccc gccatcaaga agggcatcct gcagaccgtg aaggtggtgg acgagctggt 5461 gaaggtgatg ggccgccaca agcccgagaa catcgtgatc gagatggccc gcgagaacca 5521 gaccacccag aagggccaga agaacagccg cgagcgcatg aagcgcatcg aggagggcat 5581 caaggagctg ggcagccaga tcctgaagga gcaccccgtg gagaacaccc agctgcagaa 5641 cgagaagctg tacctgtact acctgcagaa cggccgcgac atgtacgtgg accaggagct 5701 ggacatcaac cgcctgagcg actacgacgt ggaccacatc gtgccccaga gcttcctgaa 5761 ggacgacagc atcgacaaca aggtgctgac ccgcagcgac aagaaccgcg gcaagagcga 5821 caacgtgccc agcgaggagg tggtgaagaa gatgaagaac tactggcgcc agctgctgaa 5881 cgccaagctg atcacccagc gcaagttcga caacctgacc aaggccgagc gcggcggcct 5941 gagcgagctg gacaaggccg gcttcatcaa gcgccagctg gtggagaccc gccagatcac 6001 caagcacgtg gcccagatcc tggacagccg catgaacacc aagtacgacg agaacgacaa 6061 gctgatccgc gaggtgaagg tgatcaccct gaagagcaag ctggtgagcg acttccgcaa 6121 ggacttccag ttctacaagg tgcgcgagat caacaactac caccacgccc acgacgccta 6181 cctgaacgcc gtggtgggca ccgccctgat caagaagtac cccaagctgg agagcgagtt 6241 cgtgtacggc gactacaagg tgtacgacgt gcgcaagatg atcgccaaga gcgagcagga 6301 gatcggcaag gccaccgcca agtacttctt ctacagcaac atcatgaact tcttcaagac 6361 cgagatcacc ctggccaacg gcgagatccg caagcgcccc ctgatcgaga ccaacggcga 6421 gaccggcgag atcgtgtggg acaagggccg cgacttcgcc accgtgcgca aggtgctgag 6481 catgccccag gtgaacatcg tgaagaagac cgaggtgcag accggcggct tcagcaagga 6541 gagcatcctg cccaagcgca acagcgacaa gctgatcgcc cgcaagaagg actgggaccc 6601 caagaagtac ggcggcttcg acagccccac cgtggcctac agcgtgctgg tggtggccaa 6661 ggtggagaag ggcaagagca agaagctgaa gagcgtgaag gagctgctgg gcatcaccat 6721 catggagcgc agcagcttcg agaagaaccc catcgacttc ctggaggcca agggctacaa 6781 ggaggtgaag aaggacctga tcatcaagct gcccaagtac agcctgttcg agctggagaa 6841 cggccgcaag cgcatgctgg ccagcgccgg cgagctgcag aagggcaacg agctggccct 6901 gcccagcaag tacgtgaact tcctgtacct ggccagccac tacgagaagc tgaagggcag 6961 ccccgaggac aacgagcaga agcagctgtt cgtggagcag cacaagcact acctggacga 7021 gatcatcgag cagatcagcg agttcagcaa gcgcgtgatc ctggccgacg ccaacctgga 7081 caaggtgctg agcgcctaca acaagcaccg cgacaagccc atccgcgagc aggccgagaa 7141 catcatccac ctgttcaccc tgaccaacct gggcgccccc gccgccttca agtacttcga 7201 caccaccatc gaccgcaagc gctacaccag caccaaggag gtgctggacg ccaccctgat 7261 ccaccagagc atcaccggcc tgtacgagac ccgcatcgac ctgagccagc tgggcggcga 7321 cagccgcgcc gaccccaaga agaagcgcaa ggtgtgagcg gccgcatcga taaaataaaa 7381 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa 7441 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa 7501 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt 7561 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc 7621 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc 7681 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc 7741 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt 7801 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg 7861 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc 7921 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact 7981 acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg ttttttttct 8041 taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag 8101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 8161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 8221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 8281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 8341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 8401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 8461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 8521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 8581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 8641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 8701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 8761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 8821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 8881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 8941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 9001 tgcggccgca aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 9061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 9121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 9181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 9241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 9301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 9361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 9421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 9481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 9541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 9601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 9661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 9721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 9781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 9841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 9901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 10021 tttccccgaa aagtgccac //