LOCUS       Exported                7926 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  expresses mouse IL-17RC (all exons).
ACCESSION   .
VERSION     .
KEYWORDS    pCMV4Neo-murine IL-17RC(FL)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL
  TITLE     Distinct functional motifs within the IL-17 receptor regulate signal
            transduction and target gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 
            24.
  PUBMED    17456598
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2956..2985
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2992..3614
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3191..3210)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3638..3658)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3643..3972
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3823..3958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3885..3904
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4259..5062
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4322..4341)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4932..4951
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5069..5117
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(5145..5164)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5146..5164)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5178..5194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5178..5194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5191..5213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5202..5218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5226..5256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5271..5292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5409..5426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5580..6168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5660..5679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6339..7199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6962..6981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7200..7304)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7331..7786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7418..7437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7628..7649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7912..8
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7926..17
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attgttttta tttttaattt tctttcaaat acttccatcg aattcagatc tggtaccacg
      841 cgtatcgata agcttatgcc cgtgtcctgg ttcctgctgt ccttggcact gggccgaaac
      901 cctgtggtcg tctctctgga gagactgatg gagcctcagg acactgcacg ctgctctcta
      961 ggcctctcct gccacctctg ggatggtgac gtgctctgcc tgcctggaag cctccagtct
     1021 gccccaggcc ctgtgctagt gcctacccgc ctgcagacgg agctggtgct gaggtgtcca
     1081 cagaagacag attgcgccct ctgtgtccgt gtggtggtcc acttggccgt gcatgggcac
     1141 tgggcagagc ctgaagaagc tggaaagtct gattcagaac tccaggagtc taggaacgcc
     1201 tctctccagg cccaggtggt gctctccttc caggcctacc ccatcgcccg ctgtgccctg
     1261 ctggaggtcc aggtgcccgc tgacctggtg cagcctggtc agtccgtggg ttctgcggta
     1321 tttgactgtt tcgaggctag tcttggggct gaggtacaga tctggtccta cacgaagccc
     1381 aggtaccaga aagagctcaa cctcacacag cagctgcctg actgcagggg tcttgaagtc
     1441 cgggacagca tccagagctg ctgggtcctg ccctggctca atgtgtctac agatggtgac
     1501 aatgtccttc tgacactgga tgtctctgag gagcaggact ttagcttctt actgtacctg
     1561 cgtccagtcc cggatgctct caaatccttg tggtacaaaa acctgactgg acctcagaac
     1621 attactttaa accacacaga cctggttccc tgcctctgca ttcaggtgtg gtcgctagag
     1681 ccagactctg agagggtcga attctgcccc ttccgggaag atcccggtgc acacaggaac
     1741 ctctggcaca tagccaggct gcgggtactg tccccagggg tatggcagct agatgcgcct
     1801 tgctgtctgc cgggcaaggt aacactgtgc tggcaggcac cagaccagag tccctgccag
     1861 ccacttgtgc caccagtgcc ccagaagaac gccactgtga atgagccaca ggatttccag
     1921 ttggtggcag gccaccccaa cctctgtgtc caggtgagca cctgggagaa ggttcagctg
     1981 caagcgtgct tgtgggctga ctccttgggg cccttcaagg atgatatgct gttagtggag
     2041 atgaaaaccg gcctcaacaa cacatcagtc tgtgccttgg aacccagtgg ctgtacacca
     2101 ctgcccagca tggcctccac gagagctgct cgcctgggag aggagttgct gcaagacttc
     2161 cgatcacacc agtgtatgca gctgtggaac gatgacaaca tgggatcgct atgggcctgc
     2221 cccatggaca agtacatcca caggcgctgg gtcctagtat ggctggcctg cctactcttg
     2281 gctgcggcgc ttttcttctt cctccttcta aaaaaggacc gcaggaaagc ggcccgtggc
     2341 tcccgcacgg ccttgctcct ccactccgcc gacggagcgg gctacgagcg tctggtggga
     2401 gcactggcgt ccgcgttgag ccagatgcca ctgcgcgtgg ccgtggacct gtggagccgc
     2461 cgcgagctga gcgcgcacgg agccctagcc tggttccacc accagcgacg ccgtatcctg
     2521 caggagggtg gcgtggtaat ccttctcttc tcgcccgcgg ccgtggcgca gtgtcagcag
     2581 tggctgcagc tccagacagt ggagcccggg ccgcatgacg ccctcgccgc ctggctcagc
     2641 tgcgtgctac ccgatttcct gcaaggccgg gcgaccggcc gctacgtcgg ggtctacttc
     2701 gacgggctgc tgcacccaga ctctgtgccc tccccgttcc gcgtcgcccc gctcttctcc
     2761 ctgccctcgc agctgccggc tttcctggat gcactgcagg gaggctgctc cacttccgcg
     2821 gggcgacccg cggaccgggt ggaacgagtg acccaggcgc tgcggtccgc cctggacagc
     2881 tgtacttcta gctcggaagc cccaggctgc tgcgaggaat gggacctggg accctgcact
     2941 acactagaat ctagagaaca gaagctgatc tcagaggagg acctgtaacc cgggtggcat
     3001 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
     3061 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     3121 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     3181 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     3241 atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt gttgggattc
     3301 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     3361 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     3421 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     3481 taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca tgcccaaccg
     3541 gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc cactcagtag
     3601 atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca gttagggtgt
     3661 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3721 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3781 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3841 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3901 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3961 ggcttttgca aaaagctcct cgagcagtgt ggttttcaag aggaagcaaa aagcctctcc
     4021 acccaggcct ggaatgtttc cacccaatgt cgagcagtgt ggttttgcaa gaggaagcaa
     4081 aaagcctctc cacccaggcc tggaatgttt ccacccaatg tcgagcaaac cccgcccagc
     4141 gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc
     4201 acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg cagccaatat
     4261 gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4321 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4381 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4441 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4501 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4561 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4621 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4681 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4741 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     4801 gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4861 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4921 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4981 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5041 ccttcttgac gagttcttct gaggggatcg gcaataaaaa gacagaataa aacgcacggg
     5101 tgttgggtcg tttgttcgga tcgcggccgc tagcggccgt aattccctat agtgagtcgt
     5161 attaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5221 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7321 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7381 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7441 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7501 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     7561 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7621 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7681 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7741 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca
     7801 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     7861 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     7921 gacgtt
//
