LOCUS       Exported                7315 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7315)
  TITLE     Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        137..584
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    622..655
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(748..1458)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1026..1045)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1295..1313
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1455..1464
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     protein_bind    complement(3262..3295)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3404..3992
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3457..3477)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3875..3886)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4024..4500
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4223..4242)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4540..4680
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4540..4669
                     /label=AAV2 ITR
     rep_origin      4755..5210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4842..4861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5052..5073
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5227..5246)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5346..5368
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5406..5424)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5492..5596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5597..6457
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5815..5834)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6628..7216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7117..7136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   7278..92
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga
       61 gggagtggcc aactccatca ctaggggttc ctgcggccgc acgcgtgtgt ctagactgca
      121 gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg ggtgggggtg
      181 cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg
      241 cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc
      301 cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc
      361 tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc
      421 accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga
      481 taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt
      541 ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaga aggtaccgga
      601 tcctctagag tcgactccgg agaagttcct attctctaga aagtatagga acttcgcaga
      661 atggtagctg gattgtagct gctattagca atatgaaacc tcttagaagt tcctattctt
      721 caaaaggtat aggaacttcg gcgcgcctta cttgtacagc tcgtccatgc cgccggtgga
      781 gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
      841 gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct tcttggcctt
      901 gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt
      961 gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg cctcccagcc
     1021 catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc gcagcttcac
     1081 cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc
     1141 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcaagta
     1201 gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga actgagggga
     1261 caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct tggcggtctg
     1321 ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac
     1381 ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca tgttatcctc
     1441 ctcgcccttg ctcaccatgg tggcgaccgg gggatccttc aaggcctgct cgggtgcgcg
     1501 cttgtgaaaa atgaccgact gtctctgctg gtactgctgc ttgcgcctct tttttttgtc
     1561 acactggcac agcagcagca tcttgaaagt ggttctgaat gttttgttgc acagagcata
     1621 gcacacgggg ttcacggtgc tgttgatgta gcacagccag tagcccagat tccaaaaggt
     1681 tttgggtatg cagctgtcac aaaaggtgtt caccagaacc atgatgttgt atggggtcca
     1741 agtgatgatg aaggcaagca agatcgcact gagggtctgg gccgctttct tctccttgac
     1801 cagggacatc cttttccgct tagtgatctg acttctggtc ttcagagcaa acctcttggc
     1861 cagagtggct tccttgaagg acagaggtag agtggccgtg ctcttaccca ctgaggagtt
     1921 gacgtcagaa gtcttagctg tgtccacggc tgactctagc tggatgggaa gcttggagaa
     1981 gctttttgga aaactgcctc catcgtccac gctcttctgg gcctgcagct tgtcggcttt
     2041 cctctccaag tccaccatcc ccagctcctc ctcaggcacc tgcaggttgt ccgatgaggg
     2101 taacttggtg gagttgagga tggtgctgtg acccggaagc ttgagcacga tggagtagat
     2161 ggctctcgtc tcggagccaa tgtcctcctc gtcggaggag gcggagttct ccagggaggc
     2221 agcagcatca ttgttgttcc aactgtcact gctgctgtgg tcttggtcca tctgctcgga
     2281 gctgggtttc cagctcttgg ttgtgaacca gaagtggcag cggccatact tcctcctgtt
     2341 ggagcgtttc atgctttgct gttgaagttc gtaactgctg cagcttcgag aactgcccgt
     2401 ggggtggaca aagttttctg tctctgcctc tgtcccagag gcttgcaggc cagcaagctc
     2461 tttggtacgc ttttcagttt ccttatagat cctccagtat aaaatagtca taatggtgac
     2521 aggcatataa aaaccagcga tggctgtgcc aaaagtaatg gtgggctcac tgaggaactg
     2581 aatgaagcac tctcccggag gcacagttct ctttccaaca aagtattgcc agaacaagat
     2641 ggcaggagcc caaaggacaa aggagatgac ccaagccaga ccgatcatca caccggctct
     2701 ctttgttgtt cgtttggctc ggtacgtgag cggcctcgtg atggaaaagt atctgtcaaa
     2761 gctgatgacc agaagattca taacagaggc attgctggct acgcagtcaa tggcaagcca
     2821 gaggtcacag gccaagttcc ctaaggccca tcgattcatg atgatgtagg tcgtaaacag
     2881 attcattgaa atgaccccga taatcagatc ggcacaggcc aggcttaaga ggaagtagtt
     2941 gttgaccgtc ttcagctgct tgttgacctt aaatgacaca attaccagga tgttgccgat
     3001 gatggtcacc aaggccagga tgcccgttaa gaaagcgatg aagaccactt gccagacggt
     3061 atgacctccc agagggtcat cggtggtacc gtctggagag gagaaattgc cagctgctcg
     3121 agaaacattg tagctgccga aatgagtgac ggttcccggg ggcagccctg catcggaggg
     3181 gctgtgtatc caggaggagc tgatgtttgg aaacaaaggc gaggttgtac tgttattgtg
     3241 caaggtcatg gtggcgctag cgaagttcct atactttcta gagaatagga acttcttgcc
     3301 ttaacccaga aattatcact gttattcttt agaatggtgc aaagagaagt tcctatacct
     3361 tttgaagaat aggaacttcg aattcgatat caagcttatc gataatcaac ctctggatta
     3421 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3481 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3541 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3601 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     3661 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     3721 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3781 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg
     3841 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3901 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3961 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc
     4021 tacgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     4081 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     4141 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     4201 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     4261 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     4321 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga
     4381 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     4441 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     4501 ctgattttgt aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
     4561 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     4621 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     4681 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     4741 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4801 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     4861 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4921 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     4981 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5041 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     5101 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     5161 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     5221 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5281 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5341 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     5401 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5461 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5821 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     6001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     6061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     6121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     6181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     7021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     7081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7261 gccttttgct cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggcc
//
