LOCUS Exported 11315 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning. ACCESSION . VERSION . KEYWORDS pBbdCas9S(RBSmut)_arr2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11315) AUTHORS Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, Jacobs-Wagner C TITLE A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi. JOURNAL Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 10.1128/AEM.02519-20. PUBMED 33257311 REFERENCE 2 (bases 1 to 11315) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11315 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 3184..3228 /label=T5 promoter /note="bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator" protein_bind 3204..3220 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." protein_bind 3236..3252 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 3289..7395 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TILERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 7565..8356 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 8479..9067 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8968..8987 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tgaataaaat tgtaagaaaa attgaaagaa tttttttaca taaaaagtta catttattga 61 agtaatttct tacttgatac ttttctattt cattaatctt tctttcttct tttatattat 121 ttttattatt taaacactcc tttaaatcta cactcccatt tttagtaaat ctatctttaa 181 ggccccttga aactctattt tgaaatctta aatctttttt ttcttgaaag tatttataga 241 ttttttggta acattctttt tttgaaaaat taagttgata gtatatttcg gtccccaaat 301 ttacccctaa gtgtttgtga taattagttg tgactttaaa ttctttttct aatttgtaaa 361 ggtaattttg cagtgttttt agtttaacgg gtgtttgact atttctttta aggttttcat 421 taaagtaata gagtatgttt ttttgtgtgt atttcttata tgttttattt atgtacgcta 481 gtgttgagat aaggactact aatttatact gtaatttatt gtagcaagtt gtggttttac 541 tggttttgtt tatattagat aagttttcca tttatagctc cttgaatacc ggatttttta 601 tctataaagt atatatatca caattttgat ttaaaagtaa attctttttt taaaaataca 661 aaaatttttt ataaattata tagaccatga tattgtatta atactaattg attttaattt 721 aaggttgaac taaaataaac atagttttgt agaaaataat ttttcattat tttgctactt 781 gatattggat cgtaaaaata ttagggcttt acaaagttct tttaaaagag aatttagtaa 841 agccctaagt cttttaataa aaaatttttt gtaaaaaagt tggcaaaaat agtttttgct 901 atatatttat atataagaaa aaattcaata ctcaaggaga taaaaaatgg aaaacttaaa 961 caataatcca caaaaaatta atcaaataaa taagcaaact aattttattg gttttgaaaa 1021 ttttatagaa cttgataatc aagttaatca aactaaagaa aaaagtaaat taagtaagat 1081 aggtaagaaa ttacccggaa taagcagtca agtctagtgc ggccgcatgg cttgttatga 1141 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt 1201 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa 1261 aacaaagtta aacatcatga ggaggtaacg tcatggtaaa agattggatt cctatttctc 1321 atgataatta taaacaagta caaggaccat tttatcatgg aactaaagct aatttagcaa 1381 ttggtgattt actaactaca ggatttattt ctcattttga agatggtcga attcttaaac 1441 atatttattt ttcagcttta atggaaccag cagtttgggg agctgaactt gctatgtcac 1501 tatctggtct tgaaggtcgt ggttatatat atatagttga accaacagga ccatttgaag 1561 atgatccaaa tcttacaaat aaaaaatttc ctggtaatcc aacacaatct tatagaactt 1621 gtgaaccttt aagaattgtt ggtgttgttg aagattggga aggacatcct gttgaattaa 1681 taagaggaat gttagattca ttagaagatt taaaacgtcg tggtttacat gttattgaag 1741 attaacctgg cgcgccaaat aatgcccgtg tccctgttga atagggacac ggttttttaa 1801 caattttttt attcctaggc cttagtggcc ggtacccggg gatccttatt actggccgct 1861 ttctagcctg gatacttgtc tggctagttg cattagagag tcagctagag ccctagggct 1921 agctgtttgt gtattcggag ccagtgtagt ttttcttttt actaacgata cgggtaataa 1981 ttgattgcct tttacagcct gaccttgaga caattgcaaa agtctgtcca cagaggtttg 2041 acctaataat ctaaaatctt gtttgatagt agtaagtgga ggtatgtagc aactagaatc 2101 ttctgtatca tcataaccca caactgaaat atcagcccca actctaagtc cactttcagt 2161 aatagctcgc atagctccta aagccatttg atcattagca actaacatag cagtagggac 2221 aatgccttca tttaacattt gcattgtttg ctgaaatcct gacatagcag accaatctcc 2281 ttctctttct gctattggtt gtatttgatt tcttgttaaa tacttgtgcc atcctgctaa 2341 tctcaatctg gcacttactg aacttaaggg acctgcaagt agtgcaattt gttgatggcc 2401 taatgcaaca aggtgttcaa ctcctaacct tgttccatct tcgtgagaaa aaattataga 2461 gtttattggg gtttggtccg acacatccaa aaacaatgct ggaacattag tacatgctgc 2521 ttcaactgca atcgcatctt ggtcatcaag aggataatta attataagtc cactaacacg 2581 ttgagctaat aaattatgta ctgctgcctt acaagcctct acacctgatc tctcaaccat 2641 tgatacaaca acgcttgcac caagttgatc agctctcgat ttaattgcgg ctacaatttg 2701 actaggcgca tgtaatgcaa gtgatgatgt ggcaactcca attaaaagtg attgtttccc 2761 cgcaagttgc tgagcaacgc gatttggtat ataattcaac tctgccatag ctgcttcaac 2821 cttctctctt gtttttgcag atacatgact tgcttggttc acaactctcg aaaccgtttg 2881 gtagctaacc ccggcatatt cagcaacatc atacaaagta acaggtttca tggatcattc 2941 ctccatgata aaatttaaat ttctgacttt ggcaaatcct tttgcctgtt aaaaagaata 3001 gcacaaaaat taaaaaaaaa gaaacgcaaa tagcaatagc attaaaaata attattatta 3061 tttatttctt tgattaaaat caaaagcctt taatgtattt tcttgatctt tcatgcgctt 3121 aacggtaaaa tccaaggtat taagttcacc ttccactttt ctctctctat cttctctaga 3181 aaatcataaa aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg 3241 agcggataac aatttcacac agaattcatt aaagaagaga aattacatat ggataagaaa 3301 tactcaatag gcttagctat cggcacaaat agcgtcggat gggcggtgat cactgatgaa 3361 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa 3421 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc 3481 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag 3541 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct 3601 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat 3661 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat 3721 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt 3781 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta 3841 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt 3901 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat 3961 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg 4021 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta 4081 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat 4141 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat 4201 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc 4261 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca 4321 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat 4381 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat 4441 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc 4501 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga 4561 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg 4621 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg 4681 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa 4741 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat 4801 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg 4861 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag 4921 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta 4981 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa 5041 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa 5101 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc 5161 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat 5221 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga 5281 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa 5341 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt 5401 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt 5461 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt 5521 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca 5581 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc 5641 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact 5701 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg 5761 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatgccat tgttccacaa 5821 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt 5881 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga 5941 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa 6001 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact 6061 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat 6121 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct 6181 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc 6241 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt 6301 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag 6361 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac 6421 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa 6481 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc 6541 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtcca gacaggcgga 6601 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa 6661 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta 6721 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta 6781 gggatcacaa ttctggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct 6841 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt 6901 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat 6961 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag 7021 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat 7081 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat 7141 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa 7201 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt 7261 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat 7321 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag 7381 ctaggaggtg actaataaaa gcttgatcag atctcagctt ttttttgaag tgcctggcag 7441 taagttgaaa aaatttgaaa taaacttgga ctatgttaat aaaaaaatac aaattaacga 7501 acaaaaagat ttgaattatt atataattta gttaaaagca attttaaatg agggaggttt 7561 ccatatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt 7621 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga 7681 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga 7741 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga 7801 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg 7861 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc 7921 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag 7981 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga 8041 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg 8101 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac 8161 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca 8221 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt 8281 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa 8341 ggtagtcggc aaataatgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt 8401 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 8461 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 8521 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 8581 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 8641 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 8701 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 8761 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 8821 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 8881 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 8941 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 9001 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 9061 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 9121 tcacatgttc tttcctgcac tagatccccg gcaagttcat ccttagggtt ttcatatctc 9181 ttaaaaagtc aatttctgct tgattaattt cttgtggatt attgtttttg cggtttttca 9241 ttttttatct ccttgagtat tgaatttttt cttatatata aatatatagc aaaaactatt 9301 tttgccaact tttttacaaa aaatttttta ttaaaacact tagggctttg ctaaattctc 9361 ttttaaaaga acttagtaaa gccctatgga tttaagaact gattatactt acgtagtaaa 9421 aatactacag atattaatag tatagaccat atcaaaatca taatcaattt atatttatct 9481 aaaattgtag tccatacaaa atacaaaaaa attaggtttt tctaaattct attttaaaag 9541 aacttagtaa cacctaatct ctaaattact tacaaaattc taaaattaga tttttagttc 9601 ttcatatttc tttaaaagtt ttttaagaaa atctttttga ttttcaaaaa tttcatgcat 9661 cataaaacta gtaaatttgg attttttttt gtaaaaatca taactttctt gggttttaag 9721 ttgaaatctt aatggtttta ttggattttg cctagatttt tttaacgccg gactttcttt 9781 atcttttata aaattataag aatttttaat accattttct attaaataat cttcttttat 9841 aagcccctct tgcagtgcag ttgctaatct taaatactta tacgcttgag tttttgctac 9901 atcaaagtct tttataaaat caagaaaagt tttataacca tcaaatttat aatattgatt 9961 atcttttatt tcttttaaaa ttttaatatt ttccaccctg tggaatattt cttttttgaa 10021 attgtcattt aacttttcct ttagtgtctt gtaataagaa attttacttt cattaatcat 10081 taaattttgt tcaacataag tatctatctt ttttgttaaa actttaacaa cacgtttttt 10141 ttccataaaa attttccttt atttttgcaa cattttcctt aattgatata attgttacta 10201 ttttagattt tttaatatcc acttaagaat taatccctca ctagaatatt ttttattata 10261 gaattcatat acatgttttt ctaaatctat aaatttatca acaattgctt tattataggg 10321 tgtatctatc ttttcttttc ttaataatcg tctcatattc tttaaataac aacaaacact 10381 gccggtttta aacctaaatt ctatgtaata tatttttgaa aaagtatata cattttcaac 10441 cccrtttatt tcatacttta caaaaacatt atttattggt tttctataac catagtaaat 10501 gcctaaaaat ttgtccccgt ctttcatagg atatagataa agtttttgaa tctctgattt 10561 atctaataat ttttcaaaaa aaattataca ccgcttttgt ttgttactaa ctactctatg 10621 caatgccttt aatatttttg tgtgatatat atttttatca cctttttttt ctttcagcag 10681 gaagatactt tcttttggaa atttaatttt ttgttcattt attcttgcag ccttaagtcc 10741 gattttcata tttaatcctt atatagcctt tctatttaat tcttttaaac tcaataaact 10801 tttttggttt ttcattagtt ctaataattc gcaataatat gtgcaaaaca ctttattgta 10861 ttctattttc ttttggttat tcaaatattc ttttataatt ggctttagaa gttcaatatt 10921 tacttctttt tttagttgtt caataaggat attgaaaatg tttgttttta aatcttgata 10981 attttgttga gaattttctt tttttctttc aattgacttc tctaatttac gctttatatt 11041 gtttaaatcg ctatatttat gactttcaat aataaaatga ggcttaaatt tgtaattttc 11101 gtatattttt tggaaacttg tttctaattg tttagaatta tattcatttt tttcaaattc 11161 tttttgagtg ttgcatagaa tttcctttaa tttttcttgt ttttctttca tacaagattt 11221 agtaaaatgt atatttttat tttttagtaa tttaatttct attattttta tgattttaag 11281 tattttgatt atattatctt tatcaatatt taaat //