LOCUS       Exported                4025 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Enhancer reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pJW1948
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans
  JOURNAL   Genetics, 2021
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     97..114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      273..300
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      392..478
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     527..549
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     541..558
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     542..558
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             887..907
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1927..2250
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(2271..2290)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2272..2290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2295..2311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2295..2311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2413..2432)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2424..3233
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2491..2510)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3380..3968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3869..3888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
       61 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      121 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
      181 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
      241 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
      301 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
      361 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
      421 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
      481 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
      541 tgtaaaacga cggccagtct taagctcggg ccccaaataa tgatcgattt tttgcaaatt
      601 acgagcgttg tagggggcgg agcgataggt cctataggtt ttggtatatc atcattcatt
      661 cattcattgg tacattcatt tacccacctt cctctttctg agcttctctg gagttctgtg
      721 cttccttttt cccttatctt tatactgtaa tttttaactt tcaggcattg attggatccc
      781 cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat
      841 tttcaggagg acccttgctt ggagggtacc gagctcagaa aaaatgccaa agaagaagcg
      901 taaggtcctg cagatggtct ccaagggaga ggctgtcatc aaggagttca tgcgtttcaa
      961 ggtccacatg gagggatcca tgaacggaca cgagttcgag atcgagggag agggagaggg
     1021 acgtccatac gagggaaccc aaaccgccaa gctcaaggtc accaagggag gtaagtttgt
     1081 gataatccaa tttcaattcg caatggtcat cgttttttca ggaccactcc cattctcctg
     1141 ggacatcctc tccccacaat tcatgtacgg atcccgtgcc ttcatcaagc acccagccga
     1201 catcccagac tactacaagc aatccttccc agagggattc aagtgggagc gtgtgatgaa
     1261 cttcgaggta agtttttact ccgcttttaa caatggttgt ttgacatcat tttttcagga
     1321 cggaggagcc gtcaccgtca cccaagacac ctccctcgag gacggaaccc tcatctacaa
     1381 ggtcaagctc cgtggaacca acttcccacc agacggacca gtcatgcaaa agaagaccat
     1441 gggatgggag gcctccaccg agcgtctcta cccagaggac ggagtcctca aaggtaagtt
     1501 tgatgaaacg gtttcgtctt atatacacta atggtacttt tcaggagaca tcaagatggc
     1561 cctccgtctc aaggacggag gacgttacct cgccgacttc aagaccacct acaaggccaa
     1621 gaagccagtc caaatgccag gagcctacaa cgtcgaccgt aagctcgaca tcacctccca
     1681 caacgaggac tacaccgtcg tcgagcaata cgagcgttcc gagggacgtc actccaccgg
     1741 aggaatggac gagctctaca agtccggagg atccggagga accggaggat cctctctttc
     1801 tcacggattc ccaccagctg ttgctgctca agatgatgga acccttccaa tgtcttgcgc
     1861 tcaagagtct ggaatggatc gtcacccagc tgcttgcgct tccgctcgta tcaacgtcta
     1921 agataaatgc aaaatccttt caagcattcc cttcttctct atcactcttc tttctttttg
     1981 tcaaaaaatt ctctcgctaa tttatttgct tttttaatgt tattatttta tgacttttta
     2041 tagtcactga aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg attctgtctg
     2101 atgtactttc acaatctctc ttcaattcca ttttgaagtg ctttaaaccc gaaaggttga
     2161 gaaaaatgcg agcgctcaaa tatttgtatt gtgttcgttg agtgacccaa caaaaagagg
     2221 aaactttatt gtgccgccaa gaaaaaagtc tcagccatcc agctgatatc ccctatagtg
     2281 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2341 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     2401 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     2461 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     2521 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     2581 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     2641 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     2701 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2761 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2821 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2881 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2941 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     3001 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     3061 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     3121 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     3181 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     3241 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     3301 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     3361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4021 ctcac
//
