LOCUS Exported 3869 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Enhancer reporter. ACCESSION . VERSION . KEYWORDS pJW1947 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3869) AUTHORS Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD TITLE An expanded auxin-inducible degron toolkit for Caenorhabditis elegans JOURNAL Genetics, 2021 REFERENCE 2 (bases 1 to 3869) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3869 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 157..184 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 276..362 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 411..433 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 425..442 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 426..442 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS 771..791 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 1655..1978 /gene="tbb-2" /label=tbb-2 terminator /note="transcription terminator for the C. elegans tubulin beta-2 gene" primer_bind complement(1999..2018) /label=T7 /note="T7 promoter, forward primer" promoter complement(2000..2018) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2023..2039) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2023..2039) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2141..2160) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 2152..2961 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(2219..2238) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 3108..3696 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3597..3616 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3850..3867 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 61 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac 121 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc 181 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc 241 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc 301 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt 361 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg 421 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgatcg attttttgca 481 aattacgagc gttgtagggg gcggagcgat aggtcctata ggttttggta tatcatcatt 541 cattcattca ttggtacatt catttaccca ccttcctctt tctgagcttc tctggagttc 601 tgtgcttcct ttttccctta tctttatact gtaattttta actttcaggc attgattgga 661 tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga 721 acattttcag gaggaccctt gcttggaggg taccgagctc agaaaaaatg ccaaagaaga 781 agcgtaaggt cctgcagatg gtctccaagg gagaggctgt catcaaggag ttcatgcgtt 841 tcaaggtcca catggaggga tccatgaacg gacacgagtt cgagatcgag ggagagggag 901 agggacgtcc atacgaggga acccaaaccg ccaagctcaa ggtcaccaag ggaggtaagt 961 ttgtgataat ccaatttcaa ttcgcaatgg tcatcgtttt ttcaggacca ctcccattct 1021 cctgggacat cctctcccca caattcatgt acggatcccg tgccttcatc aagcacccag 1081 ccgacatccc agactactac aagcaatcct tcccagaggg attcaagtgg gagcgtgtga 1141 tgaacttcga ggtaagtttt tactccgctt ttaacaatgg ttgtttgaca tcattttttc 1201 aggacggagg agccgtcacc gtcacccaag acacctccct cgaggacgga accctcatct 1261 acaaggtcaa gctccgtgga accaacttcc caccagacgg accagtcatg caaaagaaga 1321 ccatgggatg ggaggcctcc accgagcgtc tctacccaga ggacggagtc ctcaaaggta 1381 agtttgatga aacggtttcg tcttatatac actaatggta cttttcagga gacatcaaga 1441 tggccctccg tctcaaggac ggaggacgtt acctcgccga cttcaagacc acctacaagg 1501 ccaagaagcc agtccaaatg ccaggagcct acaacgtcga ccgtaagctc gacatcacct 1561 cccacaacga ggactacacc gtcgtcgagc aatacgagcg ttccgaggga cgtcactcca 1621 ccggaggaat ggacgagctc tacaagtaag ataaatgcaa aatcctttca agcattccct 1681 tcttctctat cactcttctt tctttttgtc aaaaaattct ctcgctaatt tatttgcttt 1741 tttaatgtta ttattttatg actttttata gtcactgaaa agtttgcatc tgagtgaagt 1801 gaatgctatc aaaatgtgat tctgtctgat gtactttcac aatctctctt caattccatt 1861 ttgaagtgct ttaaacccga aaggttgaga aaaatgcgag cgctcaaata tttgtattgt 1921 gttcgttgag tgacccaaca aaaagaggaa actttattgt gccgccaaga aaaaagtctc 1981 agccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg 2041 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 2101 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 2161 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg 2221 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg 2281 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 2341 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 2401 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca 2461 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 2521 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 2581 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 2641 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt 2701 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 2761 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 2821 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 2881 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 2941 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt 3001 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg 3061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 3121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 3181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 3241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 3301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 3481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 3841 accgagcgca gcgagtcagt gagcgagga //