LOCUS Exported 12486 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector expressing Human TMPRSS2. ACCESSION . VERSION . KEYWORDS pWPI-IRES-Puro-Ak-TMPRSS2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12486) TITLE Best Lab Plasmids REFERENCE 2 (bases 1 to 12486) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12486 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 86..719 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 766..891 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1384..1617 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1802..1846 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2229..3407 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2460..3398 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3355..3375 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" misc_feature 3455..3572 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 5176..5727 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5320..5337) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5547..5566 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5730..6329 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5730..5749) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6226..6246 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(6371..6387) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6372..6388) /label=pBluescriptKS /note="For pBluescript vector" misc_feature 6401..6989 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6454..6474) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6872..6883) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind 7133..7166 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 7186..7366 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(7391..7409) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(7392..7409) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(7699..7718) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(7932..7951) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8051..8073 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8111..8129) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8197..8301 /gene="bla" /label=AmpR promoter CDS 8302..9162 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8520..8539) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9333..9921 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9822..9841 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10075..10092 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(10162..10182) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 10167..10496 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 10347..10482 /label=SV40 ori /note="SV40 origin of replication" primer_bind 10409..10428 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 11630..11695 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 11825..11845 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 12270..12404 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12307..12326) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12361..12380 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt 61 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat 121 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca 181 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt 241 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg 301 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc 361 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat 421 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg 481 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg 541 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 601 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 661 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 721 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg 781 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca 841 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag 901 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa 961 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct 1021 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt 1081 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg 1141 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa 1201 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga 1261 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt 1321 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg 1381 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc 1441 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa 1501 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa 1561 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg 1621 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg 1681 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga 1741 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga 1801 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa 1861 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg 1921 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc 1981 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga 2041 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg 2101 gtatcgatca cgagactagc ctcgaccatc gatgtcgacg ataagctttg caaagatgga 2161 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag 2221 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga 2281 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact 2341 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata 2401 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg 2461 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc 2521 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa 2581 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg 2641 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc 2701 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt 2761 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt 2821 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg 2881 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc 2941 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt 3001 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa 3061 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg 3121 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc 3181 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt 3241 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact 3301 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc 3361 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga atttcgacat 3421 ttaaatttaa ttaatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg 3481 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat 3541 tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgaggttt 3601 aaacccggga gcaggcacat ggctttgaac tcagggtcac caccagctat tggaccttac 3661 tatgaaaacc atggatacca accggaaaac ccctatcccg cacagcccac tgtggtcccc 3721 actgtctacg aggtgcatcc ggctcagtac tacccgtccc ccgtgcccca gtacgccccg 3781 agggtcctga cgcaggcttc caaccccgtc gtctgcacgc agcccaaatc cccatccggg 3841 acagtgtgca cctcaaagac taagaaagca ctgtgcatca ccttgaccct ggggaccttc 3901 ctcgtgggag ctgcgctggc cgctggccta ctctggaagt tcatgggcag caagtgctcc 3961 aactctggga tagagtgcga ctcctcaggt acctgcatca acccctctaa ctggtgtgat 4021 ggcgtgtcac actgccccgg cggggaggac gagaatcggt gtgttcgcct ctacggacca 4081 aacttcatcc ttcaggtgta ctcatctcag aggaagtcct ggcaccctgt gtgccaagac 4141 gactggaacg agaactacgg gcgggcggcc tgcagggaca tgggctataa gaataatttt 4201 tactctagcc aaggaatagt ggatgacagc ggatccacca gctttatgaa actgaacaca 4261 agtgccggca atgtcgatat ctataaaaaa ctgtaccaca gtgatgcctg ttcttcaaaa 4321 gcagtggttt ctttacgctg tatagcctgc ggggtcaact tgaactcaag ccgccagagc 4381 aggatcgtgg gcggcgagag cgcgctcccg ggggcctggc cctggcaggt cagcctgcac 4441 gtccagaacg tccacgtgtg cggaggctcc atcatcaccc ccgagtggat cgtgacagcc 4501 gcccactgcg tggaaaaacc tcttaacaat ccatggcatt ggacggcatt tgcggggatt 4561 ttgagacaat ctttcatgtt ctatggagcc ggataccaag tagaaaaagt gatttctcat 4621 ccaaattatg actccaagac caagaacaat gacattgcgc tgatgaagct gcagaagcct 4681 ctgactttca acgacctagt gaaaccagtg tgtctgccca acccaggcat gatgctgcag 4741 ccagaacagc tctgctggat ttccgggtgg ggggccaccg aggagaaagg gaagacctca 4801 gaagtgctga acgctgccaa ggtgcttctc attgagacac agagatgcaa cagcagatat 4861 gtctatgaca acctgatcac accagccatg atctgtgccg gcttcctgca ggggaacgtc 4921 gattcttgcc agggtgacag tggagggcct ctggtcactt cgaagaacaa tatctggtgg 4981 ctgatagggg atacaagctg gggttctggc tgtgccaaag cttacagacc aggagtgtac 5041 gggaatgtga tggtattcac ggactggatt tatcgacaaa tgagggcaga cggctaggga 5101 tccatatgcg gaccgtacgt actagtggcc acggctgatc atacgggctg caggaattcc 5161 gccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg 5221 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg 5281 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag 5341 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt 5401 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc 5461 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga 5521 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga 5581 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct 5641 ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg gggacgtggt 5701 tttcctttga aaaacacgat gataatacca tgaccgagta caagcccacg gtgcgcctcg 5761 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc 5821 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag 5881 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg 5941 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga 6001 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag 6061 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct 6121 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg 6181 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct 6241 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct 6301 ggtgcatgac ccgcaagccc ggtgcctgac gggcgcgtct ggaacttcga attcgatatc 6361 aagcttatcg cgataccgtc gacctcgagg gaattccgat aatcaacctc tggattacaa 6421 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 6481 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 6541 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 6601 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 6661 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 6721 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 6781 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat 6841 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 6901 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 6961 tcggatctcc ctttgggccg cctccccgca tcgggaattc gagctcggta cctttaagac 7021 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 7081 aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgg gaataacttc 7141 gtatagcata cattatacga agttatgctg ctttttgctt gtactgggtc tctctggtta 7201 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 7261 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 7321 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa 7381 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt 7441 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac 7501 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa 7561 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca 7621 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca 7681 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc 7741 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg 7801 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc 7861 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg 7921 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 7981 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 8041 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 8101 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 8161 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 8221 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 8281 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 8341 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 8401 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 8461 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 8521 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 8581 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 8641 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 8701 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 8761 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 8821 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 8881 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 8941 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 9001 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 9061 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 9121 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 9181 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 9241 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 9301 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 9361 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 9421 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt 9481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 9541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 9601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 9661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 9721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 9781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 9841 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 9901 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 9961 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 10021 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 10081 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 10141 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 10201 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 10261 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 10321 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 10381 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 10441 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc 10501 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg 10561 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat 10621 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag 10681 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat 10741 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta 10801 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg 10861 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac 10921 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta 10981 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac 11041 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg 11101 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc 11161 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca 11221 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa 11281 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa 11341 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac 11401 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt 11461 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac 11521 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg 11581 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa 11641 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc 11701 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt 11761 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac 11821 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag 11881 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac 11941 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat 12001 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag 12061 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa 12121 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata 12181 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 12241 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 12301 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 12361 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag 12421 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt 12481 ggtttc //