LOCUS Exported 12162 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION MAC-tagged gene expression. ACCESSION . VERSION . KEYWORDS MAC-SARS-CoV-2 NSP3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12162) AUTHORS Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M TITLE Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks. JOURNAL Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 10.1038/s41596-020-0365-x. PUBMED 32778839 REFERENCE 2 (bases 1 to 12162) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12162 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" protein_bind 586..604 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 607..625 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 635..659 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 802..826 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(6665..6689) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 6736..6759 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 6796..6819 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 6850..6876 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 6877..7836 /codon_start=1 /product="promiscuous R118G mutant of the E. coli biotin protein ligase (Cronan, 2005)" /label=BirA(R118G) /note="also known as BirA* or BioID" /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK" primer_bind complement(7880..7897) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7886..8110 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(8243..8262) /label=F1ori-R /note="F1 origin, reverse primer" protein_bind 8394..8441 /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." CDS 8449..9471 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE " polyA_signal 9601..9722 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9638..9657) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9692..9711 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9771..9787) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9771..9787) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9784..9806) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9795..9811 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9819..9849) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9864..9885 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10002..10019) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10173..10761) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10253..10272) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10932..11792) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11555..11574 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11793..11897) /gene="bla" /label=AmpR promoter primer_bind complement(11972..11991) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata 601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg 661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc 721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt 781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatggc ccctaccaag 841 gtgaccttcg gagacgacac cgtgatcgag gtgcagggtt acaagagcgt gaacatcacc 901 ttcgagctgg acgaacgcat cgacaaggtg ctgaacgaaa agtgctccgc ttacaccgtg 961 gagctgggca ccgaggtgaa cgaattcgcc tgcgtggtgg ccgacgctgt gatcaagacc 1021 ctgcagccag tgtccgaact gctgacccct ctgggtatcg acctggacga gtggtctatg 1081 gctacctact acctgttcga cgagagcggc gaattcaagc tggcctctca catgtactgc 1141 agcttctacc ctcccgacga agacgaggaa gagggtgact gcgaagagga agagttcgag 1201 ccttccactc agtacgagta cggcaccgaa gacgactacc agggcaagcc tctggaattc 1261 ggagccacct ctgccgctct gcagcctgaa gaggaacagg aggaagactg gctggacgac 1321 gactcccagc agaccgtggg tcagcaggac ggctctgaag acaaccagac caccaccatc 1381 cagaccatcg tggaggtgca gcctcagctg gagatggaac tgaccccagt ggtgcagacc 1441 atcgaggtga acagcttctc cggctacctg aagctgaccg acaacgtgta catcaagaac 1501 gctgacatcg tggaggaagc caagaaggtg aaaccaaccg tggtggtgaa cgccgctaac 1561 gtgtacctga agcacggcgg aggtgtggct ggagctctga acaaggctac caacaacgcc 1621 atgcaggtgg aatccgacga ctacatcgct accaacggtc ctctgaaggt gggcggatcc 1681 tgcgtgctgt ctggccacaa cctggccaag cactgcctgc acgtggtggg accaaacgtg 1741 aacaagggag aggacatcca gctgctgaag agcgcctacg aaaacttcaa ccagcacgag 1801 gtgctgctgg ctccactgct gtctgccgga atcttcggtg ctgatcccat ccacagcctg 1861 cgcgtgtgcg tggacaccgt gaggaccaac gtgtacctgg ccgtgttcga caagaacctg 1921 tacgacaagc tggtgagctc cttcctggag atgaagtccg aaaagcaggt ggagcagaag 1981 atcgccgaga tccccaagga ggaagtgaag cctttcatca ccgaatctaa gcccagcgtg 2041 gagcagagaa agcaggacga caagaagatc aaggcctgcg tggaggaagt gaccaccacc 2101 ctggaggaaa ccaagttcct gaccgagaac ctgctgctgt acatcgacat caacggcaac 2161 ctgcaccctg actccgctac cctggtgtct gacatcgaca tcaccttcct gaagaaggac 2221 gccccctaca tcgtgggcga cgtggtgcag gaaggagtgc tgaccgctgt ggtcatccct 2281 accaagaagg ccggtggcac caccgagatg ctggccaagg ctctgcgtaa ggtgccaacc 2341 gacaactaca tcaccaccta ccccggtcag ggcctgaacg gttacaccgt ggaggaagct 2401 aagaccgtgc tgaagaagtg caagtctgcc ttctacatcc tgccctccat catctctaac 2461 gagaagcagg aaatcctggg caccgtgagc tggaacctgc gcgaaatgct ggcccacgct 2521 gaggaaacca ggaagctgat gcccgtgtgc gtggagacca aggccatcgt gagcaccatc 2581 cagagaaagt acaagggcat caagatccag gagggtgtgg tggactacgg cgctcgtttc 2641 tacttctaca cctccaagac caccgtggcc tctctgatca acaccctgaa cgacctgaac 2701 gagaccctgg tgaccatgcc actgggatac gtgacccacg gtctgaacct ggaggaagcc 2761 gctagataca tgcgttccct gaaggtgcct gctaccgtga gcgtgagcag ccctgacgct 2821 gtgaccgcct acaacggata cctgacctcc tctagcaaga cccctgagga acacttcatc 2881 gaaaccatca gcctggccgg ttcctacaag gactggtctt acagcggaca gtccacccag 2941 ctgggcatcg agttcctgaa gcgcggagac aagagcgtgt actacacctc caacccaacc 3001 accttccacc tggacggtga agtgatcacc ttcgacaacc tgaagaccct gctgtctctg 3061 cgcgaggtga ggaccatcaa ggtgttcacc accgtggaca acatcaacct gcacacccag 3121 gtggtggaca tgagcatgac ctacggacag cagttcggtc ctacctacct ggacggagct 3181 gacgtgacca agatcaagcc acacaactcc cacgagggca agaccttcta cgtgctgcct 3241 aacgatgaca ccctgagagt ggaggctttc gaatactacc acaccaccga cccatccttc 3301 ctgggcaggt acatgtctgc cctgaaccac accaagaagt ggaagtaccc ccaggtgaac 3361 ggactgacca gcatcaagtg ggccgacaac aactgctacc tggccaccgc tctgctgacc 3421 ctgcagcaga tcgaactgaa gttcaaccca cccgctctgc aggacgctta ctacagagcc 3481 cgtgctggcg aggccgctaa cttctgcgct ctgatcctgg cctactgcaa caagaccgtg 3541 ggcgaactgg gagacgtgag agagaccatg agctacctgt tccagcacgc caacctggac 3601 tcctgcaagc gtgtgctgaa cgtggtgtgc aagacctgcg gccagcagca gaccaccctg 3661 aagggagtgg aagctgtgat gtacatgggt accctgtcct acgagcagtt caagaagggc 3721 gtgcagatcc cttgcacctg cggaaagcag gccaccaagt acctggtgca gcaggaaagc 3781 ccattcgtga tgatgtccgc tcctccagcc cagtacgaac tgaagcacgg caccttcacc 3841 tgcgcttccg agtacaccgg caactaccag tgcggacact acaagcacat cacctctaag 3901 gagaccctgt actgcatcga cggagccctg ctgaccaagt cctctgaata caagggtccc 3961 atcaccgacg tgttctacaa ggagaacagc tacaccacca ccatcaagcc cgtgacctac 4021 aagctggacg gcgtggtgtg caccgaaatc gaccctaagc tggacaacta ctacaagaag 4081 gacaactctt acttcaccga gcagcctatc gacctggtgc caaaccagcc ctaccctaac 4141 gccagcttcg acaacttcaa gttcgtgtgc gacaacatca agttcgctga cgacctgaac 4201 cagctgaccg gatacaagaa gcctgcctct agagagctga aggtgacctt cttcccagac 4261 ctgaacggcg acgtggtggc tatcgactac aagcactaca ccccaagctt caagaaggga 4321 gccaagctgc tgcacaagcc catcgtgtgg cacgtgaaca acgctaccaa caaggccacc 4381 tacaagccta acacctggtg catccgttgc ctgtggtcta ccaagccagt ggaaacctcc 4441 aactctttcg acgtgctgaa gagcgaggac gctcagggca tggacaacct ggcctgcgaa 4501 gacctgaagc ccgtgagcga ggaagtggtg gagaacccaa ccatccagaa ggacgtgctg 4561 gaatgcaacg tgaagaccac cgaggtggtg ggagacatca tcctgaagcc agctaacaac 4621 agcctgaaga tcaccgagga agtgggtcac accgacctga tggccgctta cgtggacaac 4681 agctccctga ccatcaagaa gcccaacgag ctgagcaggg tgctgggtct gaagaccctg 4741 gctactcacg gtctggctgc tgtgaactct gtgccatggg acaccatcgc taactacgcc 4801 aagcctttcc tgaacaaggt ggtgtccacc accaccaaca tcgtgaccag atgtctgaac 4861 cgcgtgtgca ccaactacat gccctacttc ttcaccctgc tgctgcagct gtgcaccttc 4921 accagaagca ccaactcccg tatcaaggct agcatgccta ccaccatcgc caagaacacc 4981 gtgaagagcg tgggaaagtt ctgcctggag gcttccttca actacctgaa gtctccaaac 5041 ttcagcaagc tgatcaacat catcatctgg ttcctgctgc tctccgtgtg cctgggttcc 5101 ctgatctact ctaccgccgc tctgggagtg ctgatgtcta acctgggtat gcctagctac 5161 tgcactggtt acagagaggg atacctgaac agcaccaacg tgaccatcgc cacctactgc 5221 accggcagca tcccatgctc cgtgtgcctg tctggactgg acagcctgga cacctacccc 5281 tccctggaaa ccatccagat caccatctct agcttcaagt gggacctgac cgccttcggt 5341 ctggtggctg agtggttcct ggcctacatc ctgttcacca ggttcttcta cgtgctgggc 5401 ctggccgcta tcatgcagct gttcttctcc tacttcgctg tgcacttcat cagcaactcc 5461 tggctgatgt ggctgatcat caacctggtg cagatggctc ccatctctgc catggtgcgc 5521 atgtacatct tcttcgccag cttctactac gtgtggaagt cctacgtgca cgtggtggac 5581 ggttgcaact cctctacctg catgatgtgc tacaagcgca acagggctac cagggtggag 5641 tgcaccacca tcgtgaacgg cgtgcgcagg tccttctacg tgtacgccaa cggcggcaag 5701 ggattctgca agctgcacaa ctggaactgc gtgaactgcg acaccttctg cgctggctcc 5761 accttcatct ctgacgaggt ggccagagac ctgagcctgc agttcaagcg tccaatcaac 5821 cccaccgacc agagctccta catcgtggac tctgtgaccg tgaagaacgg tagcatccac 5881 ctgtacttcg acaaggctgg ccagaagacc tacgagcgcc actctctgag ccacttcgtg 5941 aacctggaca acctgagggc caacaacacc aagggcagcc tgcctatcaa cgtgatcgtg 6001 ttcgacggaa agtccaagtg cgaggaatct agcgccaagt ccgccagcgt gtactacagc 6061 cagctgatgt gccagccaat cctgctgctg gaccaggctc tggtgtctga cgtgggagac 6121 agcgccgaag tggccgtgaa gatgttcgac gcctacgtga acaccttctc ctctaccttc 6181 aacgtgccta tggagaagct gaagaccctg gtggctactg ctgaggctga actggctaag 6241 aacgtgtccc tggacaacgt gctgagcacc ttcatctccg ccgctaggca gggtttcgtg 6301 gactctgacg tggaaaccaa agacgtggtg gagtgcctga agctgtctca ccagagcgac 6361 atcgaagtga ccggcgactc ctgcaacaac tacatgctga cctacaacaa ggtggagaac 6421 atgaccccaa gagacctggg agcttgcatc gactgctccg cccgtcacat caacgctcag 6481 gtggccaagt ctcacaacat cgccctgatc tggaacgtga aggacttcat gagcctgtcc 6541 gagcagctgc gcaagcagat caggagcgcc gctaagaaga acaacctgcc cttcaagctg 6601 acctgcgcta ccaccagaca ggtggtgaac gtggtgacca ccaagatcgc cctgaagggc 6661 ggatacccaa ctttcttgta caaagtggtt gatatcaagg ccgccgacat caccagcctg 6721 tacaagaaag tggggtggag ccatccccag ttcgagaagg gcggaggatc tggcggcgga 6781 agcggcggag gaagctggtc ccaccctcag tttgagaaag gcaccgagct gggcagcacc 6841 atggccagct acccctacga cgtgcccgac tacgccaagg acaacaccgt gcccctgaag 6901 ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg cgagacactg 6961 ggcatgagca gagccgccat caacaagcac atccagaccc tgcgggactg gggcgtggac 7021 gtgttcacag tgcccggcaa gggctacagc ctgcccgagc ccatccagct gctgaacgcc 7081 aagcagatcc tgggccagct ggatggcgga tctgtggccg tgctgcccgt gatcgacagc 7141 accaaccagt atctgctcga ccggatcggc gagctgaagt ccggcgacgc ctgtatcgcc 7201 gagtaccagc aggccggcag aggcggcagg ggcagaaagt ggttcagccc cttcggcgcc 7261 aacctgtacc tgagcatgtt ctggagactg gaacagggcc cagccgccgc tattggcctg 7321 agcctggtca tcggcatcgt gatggccgag gtgctgcgga agctgggcgc cgacaaagtg 7381 cgcgtgaagt ggcccaacga cctgtatctg caggaccgga agctggccgg catcctggtg 7441 gaactgaccg gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg catcaacatg 7501 gccatgcggc gggtggaaga gagcgtggtc aaccagggct ggatcaccct gcaggaagcc 7561 ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct gagagccgcc 7621 ctggaactgt tcgagcagga aggcctggcc ccctacctga gcagatggga gaagctggac 7681 aacttcatca accggcccgt gaaactgatc atcggcgaca aagagatctt cggcatcagc 7741 cggggcatcg acaagcaggg ggccctgctc ctggaacagg acggcatcat caagccctgg 7801 atgggcggcg agatcagcct gcggagcgcc gagaagtgat gactcgagtc tagagggccc 7861 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 7921 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 7981 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 8041 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 8101 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 8161 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 8221 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 8281 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 8341 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc 8401 ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa 8461 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg 8521 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga 8581 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg 8641 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag 8701 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa 8761 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc 8821 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact 8881 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg 8941 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc 9001 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg 9061 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc 9121 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag 9181 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat 9241 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat 9301 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg 9361 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc 9421 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta 9481 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 9541 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 9601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 9661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 9721 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 9781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 9841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 9901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 9961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 10021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 10081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 10141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 10201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 10261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 10321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 10381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 10441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 10501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 10561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 10621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 10681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 10741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 10801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 10861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 10921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 10981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 11041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 11101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 11161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 11221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 11281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 11341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 11401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 11461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 11521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 11581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 11641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 11701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 11761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 11821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 11881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 11941 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 12001 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 12061 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc 12121 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt //