LOCUS       Exported               10143 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  MAC-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    MAC-SARS-CoV-2 S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M
  TITLE     Combined proximity labeling and affinity purification-mass 
            spectrometry workflow for mapping and visualizing protein 
            interaction networks.
  JOURNAL   Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 
            10.1038/s41596-020-0365-x.
  PUBMED    32778839
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4646..4670)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4717..4740
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4777..4800
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4831..4857
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4858..5817
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5861..5878)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5867..6091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6224..6243)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6375..6422
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6430..7452
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7582..7703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7619..7638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7673..7692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7752..7768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7752..7768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7765..7787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7776..7792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7800..7830)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7845..7866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7983..8000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8154..8742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8234..8253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8913..9773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9536..9555
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9774..9878)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9953..9972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt
      781 ggtggaattc tgcagatatc aacaagtttg tacaaaaaag ttggcatgtt cgtgttcctg
      841 gtgctgctgc cactggtgag ctcccagtgc gtgaacctga ccaccagaac ccagctgcca
      901 cctgcctaca ccaactcctt caccaggggc gtgtactacc cagacaaggt gttcagatcc
      961 agcgtgctgc actctaccca ggacctgttc ctgccattct tctctaacgt gacctggttc
     1021 cacgccatcc acgtgtctgg aaccaacggc accaagagat tcgacaaccc agtgctgcca
     1081 ttcaacgacg gcgtgtactt cgcttctacc gagaagtcta acatcatcag gggttggatc
     1141 ttcggaacca ccctggactc taagacccag tctctgctga tcgtgaacaa cgctaccaac
     1201 gtggtgatca aggtgtgcga gttccagttc tgcaacgacc cattcctggg agtgtactac
     1261 cacaagaaca acaagtcttg gatggaaagc gagttcaggg tgtacagctc tgctaacaac
     1321 tgcaccttcg agtacgtgtc tcagcctttc ctgatggacc tggaaggaaa gcagggaaac
     1381 ttcaagaacc tgagagagtt cgtgttcaag aacatcgacg gttacttcaa gatatacagc
     1441 aagcacaccc caatcaacct ggtgagggac ctgccacagg gtttctctgc tctggagccc
     1501 ctggtggacc tgccaatcgg catcaacatc accaggttcc agaccctgct ggctctgcac
     1561 aggtcttacc tgaccccagg cgacagcagc tctggatgga ccgccggcgc cgctgcttac
     1621 tacgtgggat acctgcagcc aaggaccttc ctgctgaagt acaacgaaaa cggcaccatc
     1681 accgacgccg tggactgcgc tctggaccca ctgtctgaga ccaagtgcac cctgaagtct
     1741 ttcaccgtgg aaaagggaat ctaccagacc agcaacttcc gcgtgcagcc caccgagtct
     1801 atcgtgagat tcccaaacat caccaacctg tgcccattcg gagaagtgtt caacgctacc
     1861 aggttcgcca gcgtgtacgc ttggaacagg aagagaatca gcaactgcgt ggccgactac
     1921 tctgtgctgt acaactctgc ctccttctcc accttcaagt gctacggcgt gtccccaacc
     1981 aagctgaacg acctgtgctt caccaacgtg tacgctgact ccttcgtgat cagaggagac
     2041 gaagtgagac agatcgctcc cggtcagacc ggaaagatcg ccgactacaa ctacaagctg
     2101 ccagacgact tcaccggttg cgtgatcgct tggaactcta acaacctgga ctctaaggtg
     2161 ggaggcaact acaactacct gtacaggctg ttccgcaaga gcaacctgaa gcctttcgaa
     2221 agggacatca gcaccgaaat ctaccaggct ggatctaccc catgcaacgg agtggaagga
     2281 ttcaactgct acttccctct gcagtcttac ggattccagc caaccaacgg agtgggatac
     2341 cagccataca gagtggtggt gctgtctttc gagctgctgc acgctcctgc taccgtgtgc
     2401 ggaccaaaga agtctaccaa cctggtgaag aacaagtgcg tgaacttcaa cttcaacgga
     2461 ctgaccggaa ccggagtgct gaccgaatcc aacaagaagt tcctgccttt ccagcagttc
     2521 ggtagagaca tcgccgacac caccgacgct gtgagagacc cacagaccct ggaaatcctg
     2581 gacatcaccc cctgctcttt cggaggagtg agcgtgatca ccccaggaac caacacctcc
     2641 aaccaggtgg ccgtgctgta ccaggacgtg aactgcaccg aagtgccagt ggccatccac
     2701 gctgaccagc tgaccccaac ctggagagtg tacagcaccg gatctaacgt gttccagacc
     2761 cgtgccggct gcctgatcgg agccgagcac gtgaacaact cctacgaatg cgacatccca
     2821 atcggagctg gcatctgcgc ttcttaccag acccagacca acagcccaag aagggccaga
     2881 tccgtggcct ctcagagcat catcgcctac accatgagcc tgggcgctga aaacagcgtg
     2941 gcttacagca acaactctat cgccatccca accaacttca ccatctctgt gaccaccgaa
     3001 atcctgccag tgagcatgac caagaccagc gtggactgca ccatgtacat ctgcggagac
     3061 tctaccgaat gctctaacct gctgctgcag tacggcagct tctgcaccca gctgaaccgc
     3121 gctctgaccg gaatcgctgt ggagcaggac aagaacaccc aggaagtgtt cgctcaggtg
     3181 aagcagatat acaagacccc acctatcaag gacttcggag gattcaactt ctctcagatc
     3241 ctgcccgacc catctaagcc ctctaagagg tctttcatcg aagacctgct gttcaacaag
     3301 gtgaccctgg ccgacgccgg tttcatcaag cagtacggtg actgcctggg agacatcgcc
     3361 gctagagacc tgatctgcgc ccagaagttc aacggtctga ccgtgctgcc accactgctg
     3421 accgacgaga tgatcgccca gtacacctct gccctgctgg ctggaaccat cacctctggt
     3481 tggaccttcg gagctggcgc cgccctgcag atcccattcg ctatgcagat ggcctaccgt
     3541 ttcaacggta tcggagtgac ccagaacgtg ctgtacgaaa accagaagct gatcgctaac
     3601 cagttcaact ctgctatcgg aaagatccag gactccctgt cctctaccgc ttctgccctg
     3661 ggaaagctgc aggacgtggt gaaccagaac gcccaggccc tgaacaccct ggtgaagcag
     3721 ctgtcttcta acttcggagc catctcctct gtgctgaacg acatcctgtc taggctggac
     3781 aaggtggaag ccgaggtgca gatcgacaga ctgatcaccg gaagactgca gtctctgcag
     3841 acctacgtga cccagcagct gatcagggcc gctgaaatca gagcttctgc caacctggct
     3901 gccaccaaga tgtccgaatg cgtgctgggc cagtctaaga gggtggactt ctgcggaaag
     3961 ggataccacc tgatgtcctt cccacagtct gctccacacg gagtggtgtt cctgcacgtg
     4021 acctacgtgc ccgcccagga aaagaacttc accaccgccc cagctatctg ccacgacgga
     4081 aaggctcact tccccaggga gggagtgttc gtgtctaacg gaacccactg gttcgtgacc
     4141 cagaggaact tctacgagcc acagatcatc accaccgaca acaccttcgt gtctggaaac
     4201 tgcgacgtgg tgatcggaat cgtgaacaac accgtgtacg acccactgca gccagaactg
     4261 gactctttca aggaggaact ggacaagtac ttcaagaacc acacctctcc agacgtggac
     4321 ctgggagaca tctccggaat caacgcctct gtggtgaaca tccagaagga aatcgacaga
     4381 ctgaacgagg tggccaagaa cctgaacgaa tctctgatcg acctgcagga actgggaaag
     4441 tacgagcagt acatcaagtg gccctggtac atctggctgg gtttcatcgc tggtctgatc
     4501 gctatcgtga tggtgaccat catgctgtgc tgcatgacct cttgctgctc ttgcctgaag
     4561 ggatgctgct cttgcggatc ttgctgcaag ttcgacgagg acgactccga gcctgtgctg
     4621 aagggtgtga agctgcacta cacctaccca actttcttgt acaaagtggt tgatatcaag
     4681 gccgccgaca tcaccagcct gtacaagaaa gtggggtgga gccatcccca gttcgagaag
     4741 ggcggaggat ctggcggcgg aagcggcgga ggaagctggt cccaccctca gtttgagaaa
     4801 ggcaccgagc tgggcagcac catggccagc tacccctacg acgtgcccga ctacgccaag
     4861 gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt ccactctggc
     4921 gagcagctgg gcgagacact gggcatgagc agagccgcca tcaacaagca catccagacc
     4981 ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca agggctacag cctgcccgag
     5041 cccatccagc tgctgaacgc caagcagatc ctgggccagc tggatggcgg atctgtggcc
     5101 gtgctgcccg tgatcgacag caccaaccag tatctgctcg accggatcgg cgagctgaag
     5161 tccggcgacg cctgtatcgc cgagtaccag caggccggca gaggcggcag gggcagaaag
     5221 tggttcagcc ccttcggcgc caacctgtac ctgagcatgt tctggagact ggaacagggc
     5281 ccagccgccg ctattggcct gagcctggtc atcggcatcg tgatggccga ggtgctgcgg
     5341 aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg
     5401 aagctggccg gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg
     5461 atcggcgctg gcatcaacat ggccatgcgg cgggtggaag agagcgtggt caaccagggc
     5521 tggatcaccc tgcaggaagc cggcatcaac ctggaccgga acaccctggc cgccatgctg
     5581 atcagagagc tgagagccgc cctggaactg ttcgagcagg aaggcctggc cccctacctg
     5641 agcagatggg agaagctgga caacttcatc aaccggcccg tgaaactgat catcggcgac
     5701 aaagagatct tcggcatcag ccggggcatc gacaagcagg gggccctgct cctggaacag
     5761 gacggcatca tcaagccctg gatgggcggc gagatcagcc tgcggagcgc cgagaagtga
     5821 tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     5881 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5941 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6001 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6061 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6121 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6181 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6241 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6301 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6361 ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact
     6421 tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     6481 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     6541 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     6601 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     6661 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     6721 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     6781 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     6841 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     6901 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     6961 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     7021 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     7081 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     7141 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     7201 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     7261 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     7321 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     7381 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     7441 gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg
     7501 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     7561 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     7621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7681 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7741 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7801 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7861 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7921 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7981 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8041 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8101 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8161 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8221 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8281 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8341 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8401 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8461 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8521 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8581 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8641 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     8701 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8761 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8821 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8881 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8941 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9001 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9061 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9121 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9181 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9241 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9301 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9361 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9421 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9481 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9541 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9601 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9661 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9721 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9781 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9841 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9901 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     9961 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    10021 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
    10081 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
    10141 gtt
//
