LOCUS Exported 10569 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Prime editing in mammalian cells. ACCESSION . VERSION . KEYWORDS pCMV-PE2-SpG-P2A-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10569) AUTHORS Kweon J, Yoon JK, Jang AH, Shin HR, See JE, Jang G, Kim JI, Kim Y TITLE Engineered Prime Editors with PAM flexibility. JOURNAL Mol Ther. 2021 Feb 23. pii: S1525-0016(21)00089-7. doi: 10.1016/j.ymthe.2021.02.022. PUBMED 33636398 REFERENCE 2 (bases 1 to 10569) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10569 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 100..479 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 480..683 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 634..654 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 725..744 /label=T7 /note="T7 promoter, forward primer" promoter 725..743 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 751..760 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 757..2475 /codon_start=1 /product="N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)" /label=Cas9(N) /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE" CDS 7033..7053 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 7066..7131 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 7150..7206 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 7207..7923 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(7252..7273) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7513..7532) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7860..7881 /label=EGFP-C /note="EGFP, forward primer" CDS 7932..7949 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(7972..7989) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7978..8202 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(8273..8289) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8273..8289) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8286..8308) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8297..8313 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8321..8351) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8366..8387 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8504..8521) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8675..9263) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8755..8774) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9434..10294) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10057..10076 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10295..10399) /gene="bla" /label=AmpR promoter primer_bind complement(10478..10497) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt 61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt 121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc 541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt 661 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg 721 ccgctaatac gactcactat agggagagcc gccaccatgg acaagaagta cagcatcggc 781 ctggacatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc 841 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc 901 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc 961 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac 1021 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa 1081 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac 1141 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag 1201 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc 1261 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg 1321 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc 1381 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag 1441 ctgcccggcg agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg 1501 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag 1561 gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac 1621 ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg 1681 aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac 1741 caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa 1801 gagattttct tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc 1861 caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa 1921 ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc 1981 agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat 2041 ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc 2101 ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag 2161 agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc 2221 cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg 2281 cccaagcaca gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa 2341 tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc 2401 gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac 2461 ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac 2521 gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac 2581 aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac 2641 agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg 2701 aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac 2761 ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc 2821 gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc 2881 cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc 2941 ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg 3001 aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag 3061 accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc 3121 aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac 3181 gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg 3241 gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag ctttctgaag 3301 gacgactcca tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac 3361 aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac 3421 gccaagctga ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg 3481 agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca 3541 aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag 3601 ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag 3661 gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac 3721 ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc 3781 gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa 3841 atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc 3901 gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa 3961 accggggaga tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc 4021 atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag 4081 tctatcctgc ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct 4141 aagaagtacg gcggcttcct gtggcccacc gtggcctatt ctgtgctggt ggtggccaaa 4201 gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc 4261 atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa 4321 gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac 4381 ggccggaaga gaatgctggc ctctgccaag cagctgcaga agggaaacga actggccctg 4441 ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc 4501 cccgaggata atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag 4561 atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac 4621 aaagtgctgt ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat 4681 atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa gtactttgac 4741 accaccatcg accggaagca gtacagaagc accaaagagg tgctggacgc caccctgatc 4801 caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac 4861 tctggaggat ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca 4921 acaccagaga gcagtggcgg cagcagcggc ggcagcagca ccctaaatat agaagatgag 4981 tatcggctac atgagacctc aaaagagcca gatgtttctc tagggtccac atggctgtct 5041 gattttcctc aggcctgggc ggaaaccggg ggcatgggac tggcagttcg ccaagctcct 5101 ctgatcatac ctctgaaagc aacctctacc cccgtgtcca taaaacaata ccccatgtca 5161 caagaagcca gactggggat caagccccac atacagagac tgttggacca gggaatactg 5221 gtaccctgcc agtccccctg gaacacgccc ctgctacccg ttaagaaacc agggactaat 5281 gattataggc ctgtccagga tctgagagaa gtcaacaagc gggtggaaga catccacccc 5341 accgtgccca acccttacaa cctcttgagc gggctcccac cgtcccacca gtggtacact 5401 gtgcttgatt taaaggatgc ctttttctgc ctgagactcc accccaccag tcagcctctc 5461 ttcgcctttg agtggagaga tccagagatg ggaatctcag gacaattgac ctggaccaga 5521 ctcccacagg gtttcaaaaa cagtcccacc ctgtttaatg aggcactgca cagagaccta 5581 gcagacttcc ggatccagca cccagacttg atcctgctac agtacgtgga tgacttactg 5641 ctggccgcca cttctgagct agactgccaa caaggtactc gggccctgtt acaaacccta 5701 gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa tttgccagaa acaggtcaag 5761 tatctggggt atcttctaaa agagggtcag agatggctga ctgaggccag aaaagagact 5821 gtgatggggc agcctactcc gaagacccct cgacaactaa gggagttcct agggaaggca 5881 ggcttctgtc gcctcttcat ccctgggttt gcagaaatgg cagcccccct gtaccctctc 5941 accaaaccgg ggactctgtt taattggggc ccagaccaac aaaaggccta tcaagaaatc 6001 aagcaagctc ttctaactgc cccagccctg gggttgccag atttgactaa gccctttgaa 6061 ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc taacgcaaaa actgggacct 6121 tggcgtcggc cggtggccta cctgtccaaa aagctagacc cagtagcagc tgggtggccc 6181 ccttgcctac ggatggtagc agccattgcc gtactgacaa aggatgcagg caagctaacc 6241 atgggacagc cactagtcat tctggccccc catgcagtag aggcactagt caaacaaccc 6301 cccgaccgct ggctttccaa cgcccggatg actcactatc aggccttgct tttggacacg 6361 gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg ctacgctgct cccactgcct 6421 gaggaagggc tgcaacacaa ctgccttgat atcctggccg aagcccacgg aacccgaccc 6481 gacctaacgg accagccgct cccagacgcc gaccacacct ggtacacgga tggaagcagt 6541 ctcttacaag agggacagcg taaggcggga gctgcggtga ccaccgagac cgaggtaatc 6601 tgggctaaag ccctgccagc cgggacatcc gctcagcggg ctgaactgat agcactcacc 6661 caggccctaa agatggcaga aggtaagaag ctaaatgttt atactgatag ccgttatgct 6721 tttgctactg cccatatcca tggagaaata tacagaaggc gtgggtggct cacatcagaa 6781 ggcaaagaga tcaaaaataa agacgagatc ttggccctac taaaagccct ctttctgccc 6841 aaaagactta gcataatcca ttgtccagga catcaaaagg gacacagcgc cgaggctaga 6901 ggcaaccgga tggctgacca agcggcccga aaggcagcca tcacagagac tccagacacc 6961 tctaccctcc tcatagaaaa ttcatcaccc tctggcggct caaaaagaac cgccgacggc 7021 agcgaattcg agcccaagaa gaagaggaaa gtctcgagcg gaggcgacta caaagaccat 7081 gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa gggaggcggt 7141 ggaagcggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga agagaatcct 7201 ggaccgatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 7261 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 7321 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 7381 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac 7441 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 7501 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 7561 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 7621 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 7681 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 7741 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 7801 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 7861 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 7921 aagtgaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg 7981 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 8041 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 8101 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 8161 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 8221 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 8281 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 8341 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 8401 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 8461 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 8521 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 8581 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 8641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 8701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 8761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 8821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 8881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 8941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 9001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 9061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 9121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 9181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 9241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 9301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 9361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 9421 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 9481 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 9541 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 9601 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 9661 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 9721 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 9781 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 9841 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 9901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 9961 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 10021 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 10081 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 10141 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 10201 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 10261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 10321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 10381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 10441 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat 10501 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 10561 aaatttaag //