LOCUS       Exported                8907 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABEpuro-ERBB2 S310Y
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     347..369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     385..401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    445..469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4235..4259)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4296..4316)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4301..4630
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4481..4616
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4543..4562
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4640..5239
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4640..4659)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5136..5156
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5460..5929
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6059..6076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6230..6818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6310..6329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6978..7838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7601..7620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7839..7943)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             7974..8443
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    8508..8707
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
       61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
      121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
      181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
      241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
      301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
      361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
      421 cggatcccag tgtggtggta catcacaagt ttgtacaaaa aagttggcat ggagctggcg
      481 gccttgtgcc gctgggggct cctcctcgcc ctcttgcccc ccggagccgc gagcacccaa
      541 gtgtgcaccg gcacagacat gaagctgcgg ctccctgcca gtcccgagac ccacctggac
      601 atgctccgcc acctctacca gggctgccag gtggtgcagg gaaacctgga actcacctac
      661 ctgcccacca atgccagcct gtccttcctg caggatatcc aggaggtgca gggctacgtg
      721 ctcatcgctc acaaccaagt gaggcaggtc ccactgcaga ggctgcggat tgtgcgaggc
      781 acccagctct ttgaggacaa ctatgccctg gccgtgctag acaatggaga cccgctgaac
      841 aataccaccc ctgtcacagg ggcctcccca ggaggcctgc gggagctgca gcttcgaagc
      901 ctcacagaga tcttgaaagg aggggtcttg atccagcgga acccccagct ctgctaccag
      961 gacacgattt tgtggaagga catcttccac aagaacaacc agctggctct cacactgata
     1021 gacaccaacc gctctcgggc ctgccacccc tgttctccga tgtgtaaggg ctcccgctgc
     1081 tggggagaga gttctgagga ttgtcagagc ctgacgcgca ctgtctgtgc cggtggctgt
     1141 gcccgctgca aggggccact gcccactgac tgctgccatg agcagtgtgc tgccggctgc
     1201 acgggcccca agcactctga ctgcctggcc tgcctccact tcaaccacag tggcatctgt
     1261 gagctgcact gcccagccct ggtcacctac aacacagaca cgtttgagtc catgcccaat
     1321 cccgagggcc ggtatacatt cggcgccagc tgtgtgactg cctgtcccta caactacctt
     1381 tctacggacg tgggatactg caccctcgtc tgccccctgc acaaccaaga ggtgacagca
     1441 gaggatggaa cacagcggtg tgagaagtgc agcaagccct gtgcccgagt gtgctatggt
     1501 ctgggcatgg agcacttgcg agaggtgagg gcagttacca gtgccaatat ccaggagttt
     1561 gctggctgca agaagatctt tgggagcctg gcatttctgc cggagagctt tgatggggac
     1621 ccagcctcca acactgcccc gctccagcca gagcagctcc aagtgtttga gactctggaa
     1681 gagatcacag gttacctata catctcagca tggccggaca gcctgcctga cctcagcgtc
     1741 ttccagaacc tgcaagtaat ccggggacga attctgcaca atggcgccta ctcgctgacc
     1801 ctgcaagggc tgggcatcag ctggctgggg ctgcgctcac tgagggaact gggcagtgga
     1861 ctggccctca tccaccataa cacccacctc tgcttcgtgc acacggtgcc ctgggaccag
     1921 ctctttcgga acccgcacca agctctgctc cacactgcca accggccaga ggacgagtgt
     1981 gtgggcgagg gcctggcctg ccaccagctg tgcgcccgag ggcactgctg gggtccaggg
     2041 cccacccagt gtgtcaactg cagccagttc cttcggggcc aggagtgcgt ggaggaatgc
     2101 cgagtactgc aggggctccc cagggagtat gtgaatgcca ggcactgttt gccgtgccac
     2161 cctgagtgtc agccccagaa tggctcagtg acctgttttg gaccggaggc tgaccagtgt
     2221 gtggcctgtg cccactataa ggaccctccc ttctgcgtgg cccgctgccc cagcggtgtg
     2281 aaacctgacc tctcctacat gcccatctgg aagtttccag atgaggaggg cgcatgccag
     2341 ccttgcccca tcaactgcac ccactcctgt gtggacctgg atgacaaggg ctgccccgcc
     2401 gagcagagag ccagccctct gacgtccatc atctctgcgg tggttggcat tctgctggtc
     2461 gtggtcttgg gggtggtctt tgggatcctc atcaagcgac ggcagcagaa gatccggaag
     2521 tacacgatgc ggagactgct gcaggaaacg gagctggtgg agccgctgac acctagcgga
     2581 gcgatgccca accaggcgca gatgcggatc ctgaaagaga cggagctgag gaaggtgaag
     2641 gtgcttggat ctggcgcttt tggcacagtc tacaagggca tctggatccc tgatggggag
     2701 aatgtgaaaa ttccagtggc catcaaagtg ttgagggaaa acacatcccc caaagccaac
     2761 aaagaaatct tagacgaagc atacgtgatg gctggtgtgg gctccccata tgtctcccgc
     2821 cttctgggca tctgcctgac atccacggtg cagctggtga cacagcttat gccctatggc
     2881 tgcctcttag accatgtccg ggaaaaccgc ggacgcctgg gctcccagga cctgctgaac
     2941 tggtgtatgc agattgccaa ggggatgagc tacctggagg atgtgcggct cgtacacagg
     3001 gacttggccg ctcggaacgt gctggtcaag agtcccaacc atgtcaaaat tacagacttc
     3061 gggctggctc ggctgctgga cattgacgag acagagtacc atgcagatgg gggcaaggtg
     3121 cccatcaagt ggatggcgct ggagtccatt ctccgccggc ggttcaccca ccagagtgat
     3181 gtgtggagtt atggtgtgac tgtgtgggag ctgatgactt ttggggccaa accttacgat
     3241 gggatcccag cccgggagat ccctgacctg ctggaaaagg gggagcggct gccccagccc
     3301 cccatctgca ccattgatgt ctacatgatc atggtcaaat gttggatgat tgactctgaa
     3361 tgtcggccaa gattccggga gttggtgtct gaattctccc gcatggccag ggacccccag
     3421 cgctttgtgg tcatccagaa tgaggacttg ggcccagcca gtcccttgga cagcaccttc
     3481 taccgctcac tgctggagga cgatgacatg ggggacctgg tggatgctga ggagtatctg
     3541 gtaccccagc agggcttctt ctgtccagac cctgcccctg gcgctggggg catggtccac
     3601 cacaggcacc gcagctcatc taccaggagt ggcggtgggg acctgacact agggctggag
     3661 ccctctgaag aggaggcccc caggtctcca ctggcaccct ccgaaggggc tggctccgat
     3721 gtatttgatg gtgacctggg aatgggggca gccaaggggc tgcaaagcct ccccacacat
     3781 gaccccagcc ctctacagcg gtacagtgag gaccccacag tacccctgcc ctctgagact
     3841 gatggctacg ttgcccccct gacctgcagc ccccagcctg aatatgtgaa ccagccagat
     3901 gttcggcccc agcccccttc gccccgagag ggccctctgc ctgctgcccg acctgctggt
     3961 gccactctgg aaaggcccaa gactctctcc ccagggaaga atggggtcgt caaagacgtt
     4021 tttgcctttg ggggtgccgt ggagaacccc gagtacttga caccccaggg aggagctgcc
     4081 cctcagcccc accctcctcc tgccttcagc ccagccttcg acaacctcta ttactgggac
     4141 caggacccac cagagcgggg ggctccaccc agcaccttca aagggacacc tacggcagag
     4201 aacccagagt acctgggtct ggacgtgcca gtgtgaccaa ctttcttgta caaagtggtg
     4261 atgtaggaat tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg
     4321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4381 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4441 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4501 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4561 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4621 cttttgcaaa aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     4681 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4741 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4801 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4861 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4921 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4981 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     5041 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     5101 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     5161 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     5221 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     5281 acgaccccat gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     5341 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     5401 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     5461 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     5521 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     5581 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     5641 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     5701 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     5761 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     5821 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     5881 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac atgcagcatg
     5941 tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca tagttgcatt
     6001 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6901 caaaaaggat cttcacctag atccttttgc ggccgcaaat caatctaaag tatatatgag
     6961 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7021 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7081 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7141 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7201 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7261 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7321 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7381 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7441 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7501 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7561 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7621 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7681 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7741 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7801 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7861 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7921 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc agcctgaata
     7981 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     8041 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     8101 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     8161 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     8221 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     8281 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     8341 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     8401 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg
     8461 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggcaag ctggccagca
     8521 acttatctgt gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg
     8581 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
     8641 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
     8701 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
     8761 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
     8821 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
     8881 gtgtttctgt atttgtctga aaattag
//
