LOCUS Exported 22139 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs. ACCESSION . VERSION . KEYWORDS pDGE667 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 22139) AUTHORS Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U TITLE Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants. JOURNAL Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197. PUBMED 33577114 REFERENCE 2 (bases 1 to 22139) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..22139 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1062..1691 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2120..3193 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 3259..3453 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(3689..3711) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 3797..3937 /label=bom /note="basis of mobility region from pBR322" primer_bind 3852..3871 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(3952..3969) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4123..4711) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4203..4222) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4957..5748) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK" misc_feature 6269..6293 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 8706..8958 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(8971..9996) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" misc_feature 9998..10053 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" promoter complement(10089..10268) /label=NOS promoter /note="nopaline synthase promoter" CDS complement(12112..12132) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(17865..18575) /codon_start=1 /product="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mEGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE FVTAAGITLGMDELY" primer_bind complement(17904..17925) /label=EGFP-C /note="EGFP, forward primer" primer_bind 18253..18272 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 18512..18533 /label=EGFP-N /note="EGFP, reverse primer" CDS complement(18588..18614) /codon_start=1 /product="nuclear localization signal of human c-Myc proto-oncogene (Dang and Lee, 1988)" /label=c-myc NLS /translation="PAAKRVKLD" promoter 20314..20416 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 20417..21070 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG" primer_bind complement(20483..20502) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 21420..21725 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 21604..21623 /label=ccdB-fwd /note="ccdB gene, forward primer" misc_feature 21846..21870 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" ORIGIN 1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa 61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt 121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac 181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat 241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg 301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg 361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt 421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg 481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca 541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc 601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac 661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc 721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc 781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac 841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg 901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc 961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat 1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac 1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc 1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt 1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg 1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg 1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc 1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg 1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc 1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc 1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat 1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa 1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc 1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc 1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta 1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc 1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca 1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg 2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc 2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc 2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg 2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa 2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga 2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag 2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat 2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag 2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg 2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt 2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg 2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg 2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga 2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac 2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg 2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg 3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt 3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc 3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc 3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg 3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg 3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta 3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat 3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga 3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg 3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg 3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc 3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac 3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc 4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata 4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg 4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat 4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc 5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg 5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc 5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg 5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac 5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt 5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat 5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat 5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg 5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct 5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc 5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac 5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt 5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg 5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa 5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa 5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca 6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt 6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg 6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc 6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct 6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga 6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctctctgag 6361 cgactaaatg gagcaaccta ctgtttttga agtttgggtt gccatgtaat cacttgcaat 6421 aaatatatgt actaatgact gcatcggctt gcgtgtctaa atttgtagct tattgaaaac 6481 tttttgtggt taaagacgaa tccagttaac taacataagt ttaacaaaac aagacctttt 6541 ccatatatag accaccgtta ccaacttaca aagaagccaa ataaagtaca acacaacact 6601 tgcaagttac aaaaagagag gaaaaaaaac atggtaccat ttgacaacat atagaaacac 6661 acataaacat tacttataac aaaaagattc aaactcggct tttattatct ccccctgatc 6721 aaattctaaa cactttattc ctcataaact aactccccga cattggagtt attggactat 6781 gacgatgaca tcagtggggt aagctcactt gtgccccagt ttgctaggga ggtcgcagta 6841 tctggccaca gccacctcgt gctgctcgac gtaggtttct ttgtcggcct ccttgattct 6901 ttccagtctg tggtccacat agtagacgcc gggcatcttg aggttcttag cgggtttctt 6961 ggatctgtat gtggtcttga agttgcagat caggtggccc ccgcccacga gcttcagggc 7021 catgtcgctt ctgccttcca ggccgccgtc agcggggtac agcatctcgg tgttggcctc 7081 ccagccgagt gttttcttct gcatcacagg gccgttggat gggaagttca cccctctgat 7141 cttgacgttg tagatgaggc agccgtcctg gaggctggtg tcctgggtag cggtcagcac 7201 gcccccatct tcgtatgtgg tgactctctc ccatgtgaag ccctcaggga aggactgctt 7261 aaagaagtcg gggatgccct gggtgtggtt gatgaaggtt ctgctgccgt acatgaagct 7321 ggtagccagg atgtcgaagg cgaaggggag agggccgccc tcgaccacct tgattctcat 7381 ggtctgggtg ccctcgtagg gcttgccttc gccctcggat gtgcacttga agtggtggtt 7441 gttcacggtg ccctccatgt acagcttcat gtgcatgttc tccttaatca gctcgctcat 7501 agtagtgtgc tggccaccac gagtacggtc acggtcatgt tccccaccag tatgttgctg 7561 tccgtagtac tgagctctgt ctttcagatc ctgagctttg cttcccaact tcatccttgc 7621 actgtccaac ttgtctgatc cctgtgggtg ctctcccgtt gcgtacctat tcacaatcaa 7681 taggtatcat accaagcatt ttacctatat agatgatata cacagacaca ttcaagctag 7741 ctaggtcaaa tttcgtattt agttaaccaa atgttcccta gaatttgcaa tttcttattg 7801 ttacatttgt tattcgacca aaaaaattga tccaaagcta ctggctcaac acatgcatgc 7861 acatattgca caaaattatg aagtaagatg ataaatgtgt gcttacttgt aaatccaaga 7921 gaaaacggtt atagcggcaa tgccaaaccc tccagaggaa agaaaaccgg tgatgaggag 7981 tgcaactgtg atgagagccg ggacaaggat tgggctgaag ataacgagca gaggtgttgc 8041 aacagtcaaa gctatgacag ttccaacaag ggtaaggctg gagagaacaa ggagggaacc 8101 accagctgtg acagcagttg cagctttagc aatctgccta gacttggagt agtcagatcc 8161 tcgtccggac atctggtact ggtcacgatc tcggcccatc atcgggtact gatctctgcc 8221 gatgatatcg tgatgggttc ctctagctgt atccgccatt tttttgttct tgtttactag 8281 agagaatgag attttgaatg agagattgat caaaaagatg tgtatatatt ttggaagagg 8341 taggagtgtt tgttagatat attaagaaag gagacgagaa gtcacgtgtc aatggagaca 8401 tcatgcaatt gtaggccacg tgttaatggc gtcaccatgc agccgctgca tgtgttaacg 8461 agaggagtct attcaaagac acgtttgttt tttgcctagc tcagaatgtg gcgttggcat 8521 cgaaccgaaa cataaaacgc aaaacgaacc tttgggctcg tcagtatggg tttgggcctt 8581 caatagattc cttctatacg gcccacatat ccactattgg caacaaaatt agaaaaattt 8641 aggatttagt tttaaaattt gttccgcgac attctagaac tccccatggg aattcgtaaa 8701 gcgtcgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta 8761 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca 8821 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag 8881 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg 8941 ccaaatgttt gaacgatctg cttgacaagc ctattccttt gccctcggac gagtgctggg 9001 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct 9061 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca 9121 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg 9181 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg 9241 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa 9301 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc 9361 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg 9421 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga 9481 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc 9541 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa 9601 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg 9661 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg 9721 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg 9781 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca 9841 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc 9901 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt 9961 ctcgacagac gtcgcggtga gttcaggctt tttcattgta attgtaaata gtaattgtaa 10021 tgttgtttgt tgtttgttgt tgttggtaat tgttgtaaaa atacagtatg agagtgaata 10081 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa 10141 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct 10201 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt 10261 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata 10321 acgtgactcc cttaattctc cgctctccga gctcgaattc tagtagcgtc ccaaaatctt 10381 aggaacacgg aatggtacaa aagagtagta cattattgct gaatagctga aacctttgtg 10441 ttgcataaaa tttcatcatt cgtttgaaac ctttattttt ccccctaatt tgttttgtaa 10501 atagtgtaat gtttatataa agcttgtaag aatcatgtta aaattatttg taggtttctt 10561 tgtctctctg caatcgtttg aatcacaaac acctgcggtt atatattgcg tttgcgtttt 10621 ctgaagtagt agtcatgagt gacttacttg atacacaatt ttaaaatgct attacttgat 10681 accaaattac tggcgcgttt tatatacggg aatggcaaat tttgtgttgc tataccgata 10741 actgacgtga cgtggcccgt tattagtcct ggacattcgg tttcagttcg cttccattcg 10801 gttatcagtt tttctggttc taaagcttag tatccattcg gttattttga aagtttagtt 10861 cggttctagt ttttttggtt ttaaaatttt gtatccattc ggttgtttag attgaatatt 10921 tttggatatt ttggttattt ttgaatattt tcggtcattt tggatatttt tcggtcattt 10981 ttggatattt ttggatattt cggttatttt cgagtatttt tactattata tatatatttt 11041 taattattta gtctagaaat aactaatatt ttaaaaatat atttatattt cggaaagttt 11101 cgtttacccg aaatatttcg ctacgtatct atttggtttc agttctttcg attataaaat 11161 tcagtatcca ttcggttatt tccaactttc ggtacgattc ggtttagtta tttttgtttt 11221 ggtttcgatt ctgtacagtc ggttcagttt attcgcccag acctacccgt tatatcgagt 11281 aatatagaaa catttaccaa tttaaagaat caacagagat tagatgatca tatggttagg 11341 attttaaaaa gagggttttc acaggtcgtg tgaatcacac tctctattca gcatcaaaag 11401 agttttgaag ttaacgattt acaaacagag aaaagtagac gtgtaggatt tataggaggg 11461 tacaacagcg ttgtcaatca attggcaagt cataaaatgc attaaaaaat attttcatac 11521 tcaactacaa atccatgagt ataactataa ttataaagca atgattagaa tctgacaagg 11581 attctggaaa attacataaa ggaaagttca taaatgtcta aaacacaaga ggacatactt 11641 gtattcagta acatttgcag cttttctagg tctgaaaata tatttgttgc ctagtgaata 11701 agcataatgg tacaactaca agtgttttac tcctcatatt aacttcggtc attagaggcc 11761 acgatttgac acatttttac tcaaaacaaa atgtttgcat atctcttata atttcaaatt 11821 caacacacaa caaataagag aaaaaacaaa taatattaat ttgagaatga acaaaaggac 11881 catatcattc attaactctt ctccatccat ttccatttca cagttcgata gcgaaaaccg 11941 aataaaaaac acagtaaatt acaagcacaa caaatggtac aagaaaaaca gttttcccaa 12001 tgccataata ctcaaactca gtaggattct ggtgtgtgcg caatgaaact gatgcattga 12061 acttgacgaa cgttgtcgaa accgatgata cgaacgaaag ctctaagctc agactttacg 12121 cttcttcttt gggtcagccc tagagtcacc acccaactgc gacagatcta tccttgtctc 12181 atagagccct gtaatagatt ggtgaataag ggtagcgtcc aacacttcct tggttgatgt 12241 gtagcgcttt ctgtcgatag tggtatcgaa gtacttaaag gctgctgggg ctcccagatt 12301 ggtcagtgtg aacaggtgga tgatgttttc cgcttgttcc cttatcggct tatctctgtg 12361 cttgttgtac gcggaaagca cctttaattg tagaaaacca gtaaaacatg gacaattagg 12421 aggcatcgat tatctaacca aagactttaa caacactaat ctgccactaa tattcatgag 12481 aagtccttac cttatcgagg ttagcgtctg caagaatgac ccgcttggaa aattcgctga 12541 tctgctctat gatctcatca agatagtgct tgtgctgctc gacaaacaac tgcttctgct 12601 cgttgtcctc tggtgacccc ttcagcttct catagtgtga ggccaagtac aggaagttga 12661 cgtacttact gggcaatgcc agctcgttgc ccttctgcag ttctccggca gatgccagca 12721 tgcgttttct gccattttcc aattcgaaaa gcgaatactt gggcagtttg ataatgaggt 12781 ctttcttcac ctctttgtaa cctggtttgt ataattttgg ttcagttaat taggaaaatt 12841 acataatggc tccaaaaaaa aattatcgac cttttaccaa aaaaaataaa atttatcgac 12901 cttgacagga aaaagttaca aatcaaaaga aagaatatcg tctggcatcc aatattttac 12961 ctttagcctc gaggaagtcg atcggattct tctcgaacga ggacctttcc attatggtaa 13021 ttccaagcag ttccttgacg cttttcagtt tcttgctctt gcccttctcc accttggcca 13081 ctaccagtac gctgtatgcc acggtagggc tatcaaagcc tccgtatttc ttcggatccc 13141 aatccttctt cctcgcaatc agtttgtcgg agttgcgctt agggagaatg gactccttgg 13201 agaaaccacc ggtttgaacc tccgttttct tgactatgtt gacttggggc atgctgagaa 13261 ccttacgcac agtcgcaaaa tccctgccct tatcccagac aatctcacct gcaagaaaca 13321 gcaaagagag agttaagata taagtgaaca agaaagtctt gggatcgaca tacatacaat 13381 caattgatca aattcctagg attaaacata tgaaatagga aagacagacc tgtttcgcca 13441 tttgtctcga tcagaggcct cttccggatt tcaccattcg ccagagtgat ctctgtcttg 13501 aagaagttca tgatattcga gtaaaagaag tatttcgcgg tagccttgcc gatctcttgt 13561 tctgacttcg cgatcatctt cctaacgtcg tacaccttgt aatccccgta cacaaactca 13621 gactccagtt tcggatactt cttgatgaga gcagtgccaa caacggcgtt caggtaggcg 13681 tcatgggcat gatggtagtt gttgatctcg cgaaccttgt aaaactggaa gtccttccgg 13741 aagtcactaa ccagtttgct cttgagggtg atcacctgcg taaccatcaa aagtcaatat 13801 ggattcatca caaattaatc aggaatagaa agatcgggtg gccttgaagt tgatcaaata 13861 aaaagtttcg aactttagtt tgatatggta aaggaaagtt ggaaacttta cctttacctc 13921 cctgataagt ttgtcattct catcatactt cgtattcata cgcgaatcga gtatctgggc 13981 cacatgtttc gtaatctgac gagtttcaac caactggcgc ttgatgaagc cggctttgtc 14041 gagctcagac agtccgcccc tttctgcctt cgtgagattg tcgaatttcc gttgggttat 14101 gagcttagca ttcaaaagct gcctccaata gttcttcatc ttcttcacaa cctcctcgct 14161 gggtacattg tcggattttc cgcggttctt gtctgagcgc gtaaggacct ataacacggg 14221 agaaaccaaa aagcaaactc atttcacgcc aaatactaca gatttctcaa aaattaagtt 14281 ataatccata tatcctgaca agaaattgat taaaacacag ttgctttacc ttgttgtcaa 14341 tactatcgtc cttcaggaaa gattgaggga caatgtgatc cacatcatag tcggagagtc 14401 tgttaatgtc gagctcttgg tcgacgtaca tatctctccc attctggagg taatagaggt 14461 acagcttctc gttctggagt tgagtatttt cgactgggtg ctctttcaga atctgactcc 14521 caagctcctt gatgccttcc tctatccgct tcatgcgctc ccgtgagttc ttctgccctt 14581 tctgagtcgt ctggttttcc ctcgccatct cgatgacgat gttctccggc ttatggcggc 14641 ccatgacctg tagcaataat taaaacaaaa ataccacaaa cagttagaac tgaggcaaga 14701 agaatgaatg cagagaaaaa aaatcttagc tattcatgga aatggaagtg cgacgaacac 14761 ctaaaatgca aggagcataa ccaaatgcag aacttacctt caccagctca tccacaacct 14821 tcactgtctg cagtatgccc ttctttatag ctggcgatcc agccaggttg gctatgtgtt 14881 cgtgaaggct atcgccctga cctgacactt gggccttctg gatatcttct ttgaaggtca 14941 gtgagtcatc gtgaataagc tgcatgaagt tccgattcgc aaaaccgtcg gatttgagaa 15001 agtccagtat agtcttgccg gattgcttgt cgcggatgcc gttgatgagc ttcctggaga 15061 gccttcccca acctaaaaca tagaacaaac atttgtctca tcacaaatat gcatcaagga 15121 acactcagaa gttagtcata tacccaaatt cttgaaacta gagaaacaaa aaaagaggga 15181 tcattgcgga aaccttttga cctcttacct gtatatctcc tcctcttcaa ctgcttcatg 15241 accttgtcat cgaacagatg cgcataagtc ttaaggcgct cctcgatcat ttctctgtcc 15301 tcaaacagcg tgagagtcag cacgatgtcc tcgaggatgt cctcgttctc ctcgttgtca 15361 aggaagtcct tgtccttgat gattttcaac aggtcatggt atgtgccaag agaggcattg 15421 aacctatcct caacgccgct gatttccaca gaatcaaagc attcgatctt cttaaagtag 15481 tcctctttaa gctgtttcac agtgaccttc cgattggttt tgaacaagag atcgacaatg 15541 gccttcttct gctcacctgt tataacagta aaaattactt tagaaacaaa acaaaagtgt 15601 ggtcttttag gaatgattat tgatcagttc atagtttaaa atcttgtgtt tttatcgtaa 15661 aagtgtggac caggatttac ctgacaggaa ggccggtttg cgcatcccct ccgtcacata 15721 tttcaccttc gtcagctcgt tgtagaccgt gaagtattcg tacaggagac tatgcttggg 15781 caggactttc tcatttggga gattcttatc gaagttggtc atcctttcga tgaaggactg 15841 tgcactcgca cccttgtcca cgacctcttc gaagttccaa ggcgtgatag tctcctcgga 15901 tttgcgtgtc atccacgcaa accgcgagtt cccgcgtgcg agtggaccta atgaattaat 15961 agaaaaatca ggcaaattag aaaagaaata attctcaaaa gagaacaact gaggtcagaa 16021 aatagcattc atcaactgaa actaggtatt tcttcatatg atactaacct acatagtagg 16081 gaatgcggaa cgtgaggatc ttttcaatct tctctctgtt gtctttcagg aacggataga 16141 agtcctcttg cctcctcaag atagcatgca gctcaccgag gtggatctgg tgcggtatgc 16201 tgccattgtc gaaggtacgc tgcttccgaa gcaaatcttc gcggttcaat ttaacgagca 16261 gctcctctgt accgtccatc ttttccagaa taggcttgat gaatttgtag aattcctcct 16321 gacttgcacc taataatatc atcagaggaa aactcaattc ttatatctta cagaaatgaa 16381 caacaaaata catgtagcag agaggaaaac aatcataagt aacaacttac ctccatcaat 16441 gtaccctgcg tagccattct tcgattggtc aaagaaaatc tccttgtact tctcggggag 16501 ttgctggcga accaaagcct tcagaagggt cagatcctgg tgatgctcgt cgtacctctt 16561 gatcatggag gcgctaagtg gcgctttggt tatctccgtg ttgacccgca gaatgtcact 16621 gagcaagata gcgtctgaca gattcttggc agccaagaac aggtcggcat actgatcacc 16681 tacaaaagtt taaaagactc aattttaact ctacagcttc aaataaattt ccgcaaagaa 16741 attgataggt ttagacaaca aaagaagagt tccaatttca agagcaccta tttgggcgag 16801 cagattgtcc agatcatcat cgtatgtatc ctttgacagc tgcagcttcg cgtcctcggc 16861 gaggtcaaag ttgcttttga agttaggggt gaggcccaga gacagtgcaa tcagattccc 16921 gaacaagccg ttcttcttct cgcctggcaa ttgagcgatc agattctcaa gccgtctgga 16981 cttcgaaagt ctggcagaca gtattgcctt ggcgtcgaca cctgagattg tttgcaaaga 17041 aaaatctcaa agcatgtaca atctataaac ttatgatgga aaaagaaaag aaaggtaact 17101 aaggaatgtt acctgatgca ttgatggggt tctcctcgaa cagctgattg taggtttgga 17161 cgagctgaat gaacagttta tccacatcgg agttatccgg gttgaggtcc ccttcgatca 17221 agaaatggcc acgaaacttt atcatgtgcg cgagagccag gtatatgagc cgaaggtctg 17281 ccttatcggt tgagtccacc agcttcttgc gaaggtggta tatagtcgga tacttttcat 17341 gataggctac ttcgtcaaca atattcccaa agatggggtg acgctcgtgt ttcttatctt 17401 cctctaccag gaaggattcc tcgaggcgat ggaagaacga gtcgtcaacc tgcaaaatca 17461 agtttattga tcatcaagtg agtttacatg tagttaaatg ctttcagtaa tatcccaatg 17521 aagcaggata tgctttcagt acaaaataca taagcatact atatcataaa aatccttacc 17581 ttcgccatct cgttgctaaa gatctcttgg agataacaga ttctgttctt cctgcgagtg 17641 taccttctcc tcgcagtacg tttaagcctt gtcgcctccg cggtttctcc agagtcaaag 17701 agcagagctc cgatcaagtt cttcttaatg ctgtgcctat ctgtgttgcc cagcacttta 17761 aatttcttcg aaggtacctt gtattcatcg gtgataacgg cccacccaac tgagttcgta 17821 ccaatatcaa gtccaatgct gtacttcttg tccattgcag atttgtacag ctcgtccatg 17881 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg 17941 ttggggtctt tgctcagctt ggactgagta ctcaggtagt ggttgtcggg cagcagcacg 18001 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc 18061 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg 18121 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc 18181 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc 18241 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg 18301 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg 18361 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc 18421 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc 18481 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc 18541 accaccccgg tgaacagctc ctcgcccttg ctcactgcgg cagctgagtc gagtttaact 18601 cttttggcag caggagccat ggtgtggtga gagaaacaga gcgtgagctc aaatacacaa 18661 gtacgaaatc agagttatga gagaggattt ggagaaagaa gatcacctga agatcgtttg 18721 cgtcgcgcga atgagagaga cggcagagaa aacgagaagc tttaagagga cgaacaaaaa 18781 ccctaatgtc actcctttta tatagatgag aaatttaggg tttgggctct aatggagctg 18841 tctacatggg ctttcatttc tttatccagt aaagaaccgg tttaacaggt tccctggttt 18901 atacgtggtt ttagccggtt aacttacgga atattatttt tatcttaagt tacacaaatt 18961 acatcattaa tagtaaaaca aaattttatg gtgcaattta agtttttatg atgagtgaaa 19021 ttcgaataat atacaaatat tttactgttt aggtttccct aaacctactt ttagatatgt 19081 aaattaaaca tttttttttt tttggttaga accaagtcag tgatcataga actcaaaaag 19141 ctggtgtttc aattggtaca agcattttta ttgcaagata taaaaattca gaaggacata 19201 tccttgatgt atgtacatga aggaaccgat tttgactcca agctacaaag aagaagaaga 19261 atgatataca aagaaaactg catatcctca ccatgaaatt gaaagctaga ggaaggggaa 19321 taacagatac tctgcgtaga agagattcca tataaaccga taatatcctg agatagcttc 19381 ctgcaataat caaatgtttc tacactccgt tagatataca ctccattgat gttccgtgat 19441 aacgagttta tgtttgtaca aaagtatttt accttggtgt agtttgcttt ttctagtacc 19501 catgtctacc aaagatcaac aacaaagata ttagagatta atgaatttgg cagtgtcaaa 19561 tgcagaaact aaagcatata gttatgaagt tcttgtgcat tatctcaaaa gtatgtaaga 19621 gatataagag tatctcataa tgctttacct ggaaaattaa gcctgaagcc aagatcacat 19681 gtgcaggaat catcaagcta cctctaaaaa cctgttcatt gttaaaggat aagagggttg 19741 gtcttttcaa agctgcatac tgaggaaagt aacaatatcg caacaaaaat aaattggtgg 19801 cgatagttta tcaagcttct acatgcagta atggtgttcg tgctttaatt aaccgaaata 19861 gttaagacgg aaatagatga attcacctga ggcatgtaga aagctagtga tatggctgaa 19921 acataattta ctagcagaag tccagaaccg aggaatgcaa tgtttctcac tccaagtttt 19981 gttgccaggg ttgatatttg gaacctattc aagatcaatc aaaggcatga ttaactaaac 20041 atgcttgaac aaacacataa agacaatata tcaaacggag ctggttaaac attgtctttg 20101 actgtcaaat ggattgagcc aaagagcgga aagaaaatgg ttatgcgtgc agttacttac 20161 tttcgatctc cttcaacatc aggaaggtcc tttgtaatag caatgaccag tgcaaacagt 20221 gtcacaaaag atgtgatgaa cgccacaggt gcactgcaaa tagcgaatca aaagttgagc 20281 tccaagcttg aattcttgca gagaccgaga cgttgatcgg cacgtaagag gttccaactt 20341 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg 20401 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca 20461 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca 20521 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt 20581 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat 20641 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt 20701 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca 20761 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc 20821 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag 20881 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa 20941 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt 21001 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg 21061 gcaggggggg cgtaaacgcc gcgtggatcc ggcttactaa aagccagata acagtatgcg 21121 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg 21181 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc 21241 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa 21301 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga 21361 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa 21421 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac 21481 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc 21541 tgctgtcaga taaagtctcc cgtgaacttt acccagtggt gcatatcggg gatgaaagct 21601 ggcgcatgat gaccaccgat atggccagtg tgccggtttc cgttatcggg gaagaagtgg 21661 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa 21721 tataaatgtc aggctccctt atacacagcc agtcggtctc agcttctgca ggcatgcgac 21781 gtcgagctta gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca 21841 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat 21901 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 21961 ccacagggtt cccctcggga tcaaagtact ttaaagtact ttaaagtact ttaaagtact 22021 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtgt 22081 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgccctt //