LOCUS Exported 14929 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector expressing Human ACE2 and TMPRSS2. ACCESSION . VERSION . KEYWORDS pWPI-IRES-Puro-Ak-ACE2-TMPRSS2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14929) TITLE Best Lab Plasmids REFERENCE 2 (bases 1 to 14929) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14929 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 674..799 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1292..1525 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1710..1754 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" promoter 2137..3315 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2368..3306 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3263..3283 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" misc_feature 3363..3480 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 7527..8078 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7671..7688) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7898..7917 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 8081..8680 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8081..8100) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8577..8597 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(8722..8738) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8723..8739) /label=pBluescriptKS /note="For pBluescript vector" misc_feature 8752..9340 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8805..8825) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9223..9234) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" protein_bind 9484..9517 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 9537..9717 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(9742..9760) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(9743..9760) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(10050..10069) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(10283..10302) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10402..10424 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(10462..10480) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10548..10652 /gene="bla" /label=AmpR promoter CDS 10653..11513 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10871..10890) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11684..12272 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 12173..12192 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12426..12443 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(12513..12533) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 12518..12847 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 12698..12833 /label=SV40 ori /note="SV40 origin of replication" primer_bind 12760..12779 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 13981..14046 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 14176..14196 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 14621..14755 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(14658..14677) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 14712..14731 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 14923..627 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" ORIGIN 1 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg 61 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt 121 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg 181 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga 241 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca 301 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga 361 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat 421 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg 481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 601 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg 661 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg 721 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag 781 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat 841 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag 901 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca 961 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata 1021 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga 1081 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc 1141 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata 1201 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag 1261 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag 1321 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat 1381 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc 1441 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa 1501 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca 1561 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc 1621 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct 1681 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata 1741 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat 1801 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag 1861 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga 1921 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat 1981 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgaccatcga 2041 tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc 2101 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca 2161 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt 2221 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 2281 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 2341 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc 2401 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg 2461 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa 2521 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg 2581 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa 2641 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc 2701 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg 2761 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg 2821 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc 2881 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg 2941 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg 3001 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac 3061 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg 3121 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg 3181 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt 3241 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca 3301 tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg gtatcggtta 3361 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 3421 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt 3481 atcgatcacg agactagcct cgaggtttaa acccgggagc gccaccatgt caagctcttc 3541 ctggctcctt ctcagccttg ttgctgtaac tgctgctcag tccaccattg aggaacaggc 3601 caagacattt ttggacaagt ttaaccacga agccgaagac ctgttctatc aaagttcact 3661 tgcttcttgg aattataaca ccaatattac tgaagagaat gtccaaaaca tgaataatgc 3721 tggggacaaa tggtctgcct ttttaaagga acagtccaca cttgcccaaa tgtatccact 3781 acaagaaatt cagaatctca cagtcaagct tcagctgcag gctcttcagc aaaatgggtc 3841 ttcagtgctc tcagaagaca agagcaaacg gttgaacaca attctaaata caatgagcac 3901 catctacagt actggaaaag tttgtaaccc agataatcca caagaatgct tattacttga 3961 accaggtttg aatgaaataa tggcaaacag tttagactac aatgagaggc tctgggcttg 4021 ggaaagctgg agatctgagg tcggcaagca gctgaggcca ttatatgaag agtatgtggt 4081 cttgaaaaat gagatggcaa gagcaaatca ttatgaggac tatggggatt attggagagg 4141 agactatgaa gtaaatgggg tagatggcta tgactacagc cgcggccagt tgattgaaga 4201 tgtggaacat acctttgaag agattaaacc attatatgaa catcttcatg cctatgtgag 4261 ggcaaagttg atgaatgcct atccttccta tatcagtcca attggatgcc tccctgctca 4321 tttgcttggt gatatgtggg gtagattttg gacaaatctg tactctttga cagttccctt 4381 tggacagaaa ccaaacatag atgttactga tgcaatggtg gaccaggcct gggatgcaca 4441 gagaatattc aaggaggccg agaagttctt tgtatctgtt ggtcttccta atatgactca 4501 aggattctgg gaaaattcca tgctaacgga cccaggaaat gttcagaaag cagtctgcca 4561 tcccacagct tgggacctgg ggaagggcga cttcaggatc cttatgtgca caaaggtgac 4621 aatggacgac ttcctgacag ctcatcatga gatggggcat atccagtatg atatggcata 4681 tgctgcacaa ccttttctgc taagaaatgg agctaatgaa ggattccatg aagctgttgg 4741 ggaaatcatg tcactttctg cagccacacc taagcattta aaatccattg gtcttctgtc 4801 acccgatttt caagaagaca atgaaacaga aataaacttc ctgctcaaac aagcactcac 4861 gattgttggg actctgccat ttacttacat gttagagaag tggaggtgga tggtctttaa 4921 aggggaaatt cccaaagacc agtggatgaa aaagtggtgg gagatgaagc gagagatagt 4981 tggggtggtg gaacctgtgc cccatgatga aacatactgt gaccccgcat ctctgttcca 5041 tgtttctaat gattactcat tcattcgata ttacacaagg accctttacc aattccagtt 5101 tcaagaagca ctttgtcaag cagctaaaca tgaaggccct ctgcacaaat gtgacatctc 5161 aaactctaca gaagctggac agaaactgtt caatatgctg aggcttggaa aatcagaacc 5221 ctggacccta gcattggaaa atgttgtagg agcaaagaac atgaatgtaa ggccactgct 5281 caactacttt gagcccttat ttacctggct gaaagaccag aacaagaatt cttttgtggg 5341 atggagtacc gactggagtc catatgcaga ccaaagcatc aaagtgagga taagcctaaa 5401 atcagctctt ggagataaag catatgaatg gaacgacaat gaaatgtacc tgttccgatc 5461 atctgttgca tatgctatga ggcagtactt tttaaaagta aaaaatcaga tgattctttt 5521 tggggaggag gatgtgcgag tggctaattt gaaaccaaga atctccttta atttctttgt 5581 cactgcacct aaaaatgtgt ctgatatcat tcctagaact gaagttgaaa aggccatcag 5641 gatgtcccgg agccgtatca atgatgcttt ccgtctgaat gacaacagcc tagagtttct 5701 ggggatacag ccaacacttg gacctcctaa ccagccccct gtttccatat ggctgattgt 5761 ttttggagtt gtgatgggag tgatagtggt tggcattgtc atcctgatct tcactgggat 5821 cagagatcgg aagaagaaaa ataaagcaag aagtggagaa aatccttatg cctccatcga 5881 tattagcaaa ggagaaaata atccaggatt ccaaaacact gatgatgttc agacctcctt 5941 ttagcgcgcc tgcaggatca gcaggcacca tggctttgaa ctcagggtca ccaccagcta 6001 ttggacctta ctatgaaaac catggatacc aaccggaaaa cccctatccc gcacagccca 6061 ctgtggtccc cactgtctac gaggtgcatc cggctcagta ctacccgtcc cccgtgcccc 6121 agtacgcccc gagggtcctg acgcaggctt ccaaccccgt cgtctgcacg cagcccaaat 6181 ccccatccgg gacagtgtgc acctcaaaga ctaagaaagc actgtgcatc accttgaccc 6241 tggggacctt cctcgtggga gctgcgctgg ccgctggcct actctggaag ttcatgggca 6301 gcaagtgctc caactctggg atagagtgcg actcctcagg tacctgcatc aacccctcta 6361 actggtgtga tggcgtgtca cactgccccg gcggggagga cgagaatcgg tgtgttcgcc 6421 tctacggacc aaacttcatc cttcaggtgt actcatctca gaggaagtcc tggcaccctg 6481 tgtgccaaga cgactggaac gagaactacg ggcgggcggc ctgcagggac atgggctata 6541 agaataattt ttactctagc caaggaatag tggatgacag cggatccacc agctttatga 6601 aactgaacac aagtgccggc aatgtcgata tctataaaaa actgtaccac agtgatgcct 6661 gttcttcaaa agcagtggtt tctttacgct gtatagcctg cggggtcaac ttgaactcaa 6721 gccgccagag caggatcgtg ggcggcgaga gcgcgctccc gggggcctgg ccctggcagg 6781 tcagcctgca cgtccagaac gtccacgtgt gcggaggctc catcatcacc cccgagtgga 6841 tcgtgacagc cgcccactgc gtggaaaaac ctcttaacaa tccatggcat tggacggcat 6901 ttgcggggat tttgagacaa tctttcatgt tctatggagc cggataccaa gtagaaaaag 6961 tgatttctca tccaaattat gactccaaga ccaagaacaa tgacattgcg ctgatgaagc 7021 tgcagaagcc tctgactttc aacgacctag tgaaaccagt gtgtctgccc aacccaggca 7081 tgatgctgca gccagaacag ctctgctgga tttccgggtg gggggccacc gaggagaaag 7141 ggaagacctc agaagtgctg aacgctgcca aggtgcttct cattgagaca cagagatgca 7201 acagcagata tgtctatgac aacctgatca caccagccat gatctgtgcc ggcttcctgc 7261 aggggaacgt cgattcttgc cagggtgaca gtggagggcc tctggtcact tcgaagaaca 7321 atatctggtg gctgataggg gatacaagct ggggttctgg ctgtgccaaa gcttacagac 7381 caggagtgta cgggaatgtg atggtattca cggactggat ttatcgacaa atgagggcag 7441 acggctaggg atccatatgc ggaccgtacg tactagtggc cacggctgat catacgggct 7501 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg 7561 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc 7621 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa 7681 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag 7741 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg 7801 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg 7861 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa 7921 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg 7981 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac 8041 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atgaccgagt acaagcccac 8101 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt 8161 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac 8221 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 8281 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 8341 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 8401 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 8461 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 8521 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 8581 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 8641 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgggcgcgtc tggaacttcg 8701 aattcgatat caagcttatc gcgataccgt cgacctcgag ggaattccga taatcaacct 8761 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 8821 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 8881 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 8941 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 9001 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 9061 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 9121 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 9181 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 9241 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 9301 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cgagctcggt 9361 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 9421 ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg 9481 ggaataactt cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt 9541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 9601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 9661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca 9721 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa 9781 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc 9841 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt 9901 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga 9961 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat 10021 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga 10081 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 10141 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt 10201 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga 10261 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 10321 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 10381 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 10441 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 10501 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 10561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 10621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 10681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 10741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 10801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 10861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 10921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 10981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 11041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 11101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 11161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 11221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 11281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 11341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 11401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 11461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 11521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 11581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 11641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 11701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 11761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 11821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 11881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 11941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 12001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 12061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 12121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 12181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 12241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 12301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 12361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 12421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 12481 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa 12541 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 12601 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 12661 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 12721 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 12781 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 12841 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc 12901 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta 12961 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg 13021 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt 13081 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca 13141 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat 13201 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa 13261 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga 13321 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat 13381 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga 13441 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg 13501 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac 13561 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa 13621 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc 13681 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc 13741 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg 13801 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg 13861 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa 13921 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag 13981 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta 14041 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag 14101 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct 14161 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca 14221 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct 14281 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct 14341 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca 14401 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt 14461 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat 14521 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 14581 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 14641 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 14701 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg 14761 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc 14821 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta 14881 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaagg //