LOCUS Exported 13243 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pRR-MR-5Z SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13243) AUTHORS Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S TITLE Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. JOURNAL Plasmid. 2009 Dec 3. ():. PUBMED 19962400 REFERENCE 2 (bases 1 to 13243) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13243 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(36..710) /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /label=TRP1 /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" promoter complement(711..858) /gene="S. cerevisiae TRP1" /label=TRP1 promoter promoter 864..894 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 902..918 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 926..942 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" terminator 992..1179 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" promoter 1349..2013 /gene="GAL1 and GAL10 genes of S. cerevisiae" /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" protein_bind 1565..1682 /label=UAS /bound_moiety="Gal4" /note="upstream activating sequence mediating Gal4-dependent induction" primer_bind 1977..2000 /label=GAL1 /note="S. cerevisiae GAL1 promoter, forward primer" primer_bind complement(5376..5395) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 5495..5517 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(5555..5573) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 5614..6117 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 6154..6258 /gene="bla" /label=AmpR promoter CDS 6259..7119 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6477..6496) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7290..7878 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7779..7798 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8032..8049 /label=L4440 /note="L4440 vector, forward primer" protein_bind 8166..8187 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 8202..8232 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8240..8256 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8264..8280 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" terminator 12199..12386 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(12538..12557) /label=T7 /note="T7 promoter, forward primer" promoter complement(12539..12557) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(12564..12581) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(12564..12580) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(12573..12595) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 12721..13176 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(12808..12827) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 13018..13039 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa 61 tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca 121 ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct 181 aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga aatcgagttc 241 caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga 301 ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta 361 ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg 421 acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga 481 aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt 541 gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata 601 cccagcaagt cagcatcgga atctagagca cattctgcgg cctctgtgct ctgcaagccg 661 caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct 721 gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct 781 tggccctctc cttttctttt ttcgaccgaa ttaattctta atcggcaaaa aaagaaaagc 841 tccggatcaa gattgtacca ggctttacac tttatgcttc cggctcgtat gttgtgtgga 901 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt 961 ttcatagggt aggggaattt cgaccggcat gccggtagag gtgtggtcaa taagagcgac 1021 ctcatgctat acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa 1081 ttttcgtttt aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac 1141 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tgtcaacaac 1201 gtatctacca acgatttgac ccttttccat cttttcgtaa atttctggca aggtagacaa 1261 gccgacaacc ttgattggag acttgaccaa acctctggcg aagaagtcca aagcttgcat 1321 gcctgcaggt cgactctaga ggatcccctt tcaaaaattc ttactttttt tttggatgga 1381 cgcaaagaag tttaataatc atattacatg gcattaccac catatacata tccatataca 1441 tatccatatc taatcttact tatatgttgt ggaaatgtaa agagccccat tatcttagcc 1501 taaaaaaacc ttctctttgg aactttcagt aatacgctta actgctcatt gctatattga 1561 agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact ctcctccgtg 1621 cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc 1681 cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt 1741 aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg 1801 cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc 1861 tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac tttcaacatt 1921 ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata 1981 tacctctata ctttaacgtc aaggagaaaa aacccgggat ggaagttcaa ttgggtttga 2041 gagtttaccc aatggagacc aaaggctacc acagtctccc tgaaggtcta gatatggaaa 2101 gacggtgggg tcaagtttct caggctgtgg agcgttcttc cctgggacct acagagagga 2161 ccgatgagaa taactacatg gagattgtca acgtaagctg tgtttccggt gctattccaa 2221 acaacagtac tcaaggaagc agcaaagaaa aacaagaact actcccttgc cttcagcaag 2281 acaataatcg gcctgggatt ttaacatctg atattaaaac tgagctggaa tctaaggaac 2341 tttcagcaac tgtagctgag tccatgggtt tatatatgga ttctgtaaga gatgctgact 2401 attcctatga gcagcagaac caacaaggaa gcatgagtcc agctaagatt tatcagaatg 2461 ttgaacagct ggtgaaattt tacaaaggaa atggccatcg tccttccact ctaagttgtg 2521 tgaacacgcc cttgagatca tttatgtctg actctgggag ctccgtgaat ggtggcgtca 2581 tgcgcgccat tgttaaaagc cctatcatgt gtcatgagaa aagcccgtct gtttgcagcc 2641 ctctgaacat gacatcttcg gtttgcagcc ctgctggaat caactctgtg tcctccacca 2701 cagccagctt tggcagtttt ccagtgcaca gcccaatcac ccagggaact cctctgacat 2761 gctcccctaa tgctgaaaat cgaggctcca ggtcgcacag ccctgcacat gctagcaatg 2821 tgggctctcc tctctcaagt ccgttaagta gcatgaaatc ctcaatttcc agccctccaa 2881 gtcactgcag tgtaaaatct ccagtctcca gtcccaataa tgtcactctg agatcctctg 2941 tgtctagccc tgcaaatatt aacaactcaa ggtgctctgt ttccagccct tcgaacacta 3001 ataacagatc cacgctttcc agtccggcag ccagtactgt gggatctatc tgtagccctg 3061 taaacaatgc cttcagctac actgcctctg gcacctctgc tggatccagt acattgcggg 3121 atgtggttcc cagtccagac acgcaggaga aaggtgctca agaggtccct tttcctaaga 3181 ctgaggaagt agagagtgcc atctcaaatg gtgtgactgg ccagcttaat attgtccagt 3241 acataaaacc agaaccagat ggagctttta gcagctcatg tctaggagga aatagcaaaa 3301 taaattcgga ttcttcattc tcagtaccaa taaagcaaga atcaaccaag cattcatgtt 3361 caggcacctc ttttaaaggg aatccaacag taaacccgtt tccatttatg gatggctcgt 3421 atttttcctt tatggatgat aaagactatt attccctatc aggaatttta ggaccacctg 3481 tgcccggctt tgatggtaac tgtgaaggca gcggattccc agtgggtatt aaacaagaac 3541 cagatgacgg gagctattac ccagaggcca gcatcccttc ctctgctatt gttggggtgc 3601 attcaggtgg acagtccttc cactacagga ttggtgctca aggtacaata tctttatcac 3661 gatcggctag agaccaatct ttccaacacc tgagttcctt tcctcctgtc aatactttag 3721 tggagtcatg gaaatcacac ggcgacctgt cgtctagaag aagtgatggg tatccggtct 3781 tagaatacat tccagaaaat gtatcaagct ctactttacg aagtgtttct actggatctt 3841 caagaccttc aaaaatatgt ttggtgtgtg gggatgaggc ttcaggatgc cattatgggg 3901 tagtcacctg tggcagctgc aaagttttct tcaaaagagc agtggaaggg caacacaact 3961 atttatgtgc tggaagaaat gattgcatca ttgataagat tcgacgaaag aattgtcctg 4021 cttgcagact tcagaaatgt cttcaagctg gaatgaattt aggagcacga aagtcaaaga 4081 agttgggaaa gttaaaaggg attcacgagg agcagccaca gcagcagcag cccccacccc 4141 cacccccacc cccgcaaagc ccagaggaag ggacaacgta catcgctcct gcaaaagaac 4201 cctcggtcaa cacagcactg gttcctcagc tctccacaat ctcacgagcg ctcacacctt 4261 cccccgttat ggtccttgaa aacattgaac ctgaaattgt atatgcaggc tatgacagct 4321 caaaaccaga tacagccgaa aatctgctct ccacgctcaa ccgcttagca ggcaaacaga 4381 tgatccaagt cgtgaagtgg gcaaaggtac ttccaggatt taaaaacttg cctcttgagg 4441 accaaattac cctaatccgg tattcttgga tgtgtctatc atcatttgcc ttgagctgga 4501 gatcgtacaa acatacgaac agccaatttc tctattttgc accagaccta gtctttaatg 4561 aagagaagat gcatcagtct gccatgtatg aactatgcca ggggatgcac caaatcagcc 4621 ttcagttcgt tcgactgcag ctcacctttg aagaatacac catcatgaaa gttttgctgc 4681 tactaagcac aattccaaag gatggcctca aaagccaggc tgcatttgaa gaaatgagga 4741 caaattacat caaagaactg aggaagatgg taactaagtg tcccaacaat tctgggcaga 4801 gctggcagag gttctaccaa ctgaccaagc tgctggactc catgcatgac ctggtgagcg 4861 acctgctgga attctgcttc tacaccttcc gagagtccca tgcgctgaag gtagagttcc 4921 ccgcaatgct ggtggagatc atcagcgacc agctgcccaa ggtggagtcg gggaacgcca 4981 agccgctcta cttcctccgg aagtgaggta ccgagctcga attcgacact ctaaataagc 5041 ggatctctta tgtctttacg atttatagtt ttcattatca agtatgccta tattagtata 5101 tagcatcttt agatgacagt gttcgaagtt tcacgaataa aagataatat tctacttttt 5161 gctcccaccg cgtttgctag cacgagtgaa caccatccct cgcctgtgag ttgtacccat 5221 tcgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc atctggcgtc 5281 ataactgcaa agtacacata tattacgatg ctgttctatt aaatgcttcc tatattatat 5341 atatagtaat gtcgtgatct atggtgcact ctcagtacaa tctgctctga tgccgcatag 5401 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc 5461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt 5521 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag 5581 gttaatgtca tgataataat ggtttcttag acggatcgct tgcctgtaac ttacacgcgc 5641 ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg 5701 ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa 5761 aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat 5821 tagacaagaa aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa 5881 tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata 5941 cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt 6001 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt 6061 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa 6121 aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 6181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 6241 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 6301 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 6361 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 6421 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 6481 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 6541 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 6601 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 6661 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 6721 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 6781 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 6841 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 6901 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 6961 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 7021 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 7081 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 7141 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 7201 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 7261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 7321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 7381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 7441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 7501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 7561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 7621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 7681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 7741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 7801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 7861 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 7921 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 7981 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 8041 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 8101 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 8161 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 8221 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 8281 atgattacga attaattcgc tagagtcgag gccgagtttg tcagaaagca gaccaaacag 8341 cggttggaat aatagcgaga acagagaaat agcggcaaaa ataatacccg tatcactttt 8401 gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac gggaagtagg ctcccatgat 8461 aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag tttgtgtttt ttaaatagta 8521 cataatggat ttccttacgc gaaatacggg cagacatggc ctgcccggtt attattattt 8581 ttgacaccag accaactggt aatggtagcg accggcgctc agctgtaatt ccgccgatac 8641 tgacgggctc caggagtcgt cgccaccaat ccccatatgg aaaccgtcga tattcagcca 8701 tgtgccttct tccgcgtgca gcagatggcg atggctggtt tccatcagtt gctgttgact 8761 gtagcggctg atgttgaact ggaagtcgcc gcgccactgg tgtgggccat aattcaattc 8821 gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg tacggggtat acatgtctga 8881 caatggcaga tcccagcggt caaaacaggc ggcagtaagg cggtcgggat agttttcttg 8941 cggccctaat ccgagccagt ttacccgctc tgctacctgc gccagctggc agttcaggcc 9001 aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca tcaacggtaa tcgccatttg 9061 accactacca tcaatccggt aggttttccg gctgataaat aaggttttcc cctgatgctg 9121 ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt gtatctgccg tgcactgcaa 9181 caacgctgct tcggcctggt aatggcccgc cgccttccag cgttcgaccc aggcgttagg 9241 gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc agcggtgcac gggtgaactg 9301 atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc cacatctgtg aaagaaagcc 9361 tgactggcgg ttaaattgcc aacgcttatt acccagctcg atgcaaaaat ccatttcgct 9421 ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc gtcacactga ggttttccgc 9481 cagacgccac tgctgccagg cgctgatgtg cccggcttct gaccatgcgg tcgcgttcgg 9541 ttgcactacg cgtactgtga gccagagttg cccggcgctc tccggctgcg gtagttcagg 9601 cagttcaatc aactgtttac cttgtggagc gacatccaga ggcacttcac cgcttgccag 9661 cggcttacca tccagcgcca ccatccagtg caggagctcg ttatcgctat gacggaacag 9721 gtattcgctg gtcacttcga tggtttgccc ggataaacgg aactggaaaa actgctgctg 9781 gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg gcaaagacca gaccgttcat 9841 acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg ccgtaagccg accacgggtt 9901 gccgttttca tcatatttaa tcagcgactg atccacccag tcccagacga agccgccctg 9961 taaacgggga tactgacgaa acgcctgcca gtatttagcg aaaccgccaa gactgttacc 10021 catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc tctccaggta gcgaaagcca 10081 ttttttgatg gaccatttcg gcacagccgg gaagggctgg tcttcatcca cgcgcgcgta 10141 catcgggcaa ataatatcgg tggccgtggt gtcggctccg ccgccttcat actgcaccgg 10201 gcgggaagga tcgacagatt tgatccagcg atacagcgcg tcgtgattag cgccgtggcc 10261 tgattcattc cccagcgacc agatgatcac actcgggtga ttacgatcgc gctgcaccat 10321 tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga tcatcggtca gacgattcat 10381 tggcaccatg ccgtgggttt caatattggc ttcatccacc acatacaggc cgtagcggtc 10441 gcacagcgtg taccacagcg gatggttcgg ataatgcgaa cagcgcacgg cgttaaagtt 10501 gttctgcttc atcagcagga tatcctgcac catcgtctgc tcatccatga cctgaccatg 10561 cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag 10621 cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat 10681 cagcgtgccg tcggcggtgt gcagttcaac caccgcacga tagagattcg ggatttcggc 10741 gctccacagt ttcgggtttt cgacgttcag acgtagtgtg acgcgatcgg cataaccacc 10801 acgctcatcg ataatttcac cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc 10861 ctgccataaa gaaactgtta cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc 10921 agcctccagt acagcgcggc tgaaatcatc attaaagcga gtggcaacat ggaaatcgct 10981 gatttgtgta gtcggtttat gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca 11041 catatcctga tcttccagat aactgccgtc actccagcgc agcaccatca ccgcgaggcg 11101 gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca gacggcaaac gactgtcctg 11161 gccgtaaccg acccagcgcc cgttgcacca cagatgaaac gccgagttaa cgccatcaaa 11221 aataattcgc gtctggcctt cctgtagcca gctttcatca acattaaatg tgagcgagta 11281 acaacccgtc ggattctccg tgggaacaaa cggcggattg accgtaatgg gataggtcac 11341 gttggtgtag atgggcgcat cgtaaccgtg catctgccag tttgagggga cgacgacagt 11401 atcggcctca ggaagatcgc actccagcca gctttccggc accgcttctg gtgccggaaa 11461 ccaggcaaag cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 11521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagtcg 11581 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 11641 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gccaagctcc 11701 ggatccggtc attattaatt tagtgtgtgt atttgtgttt gcgtgtctat agaagtatag 11761 taatttatgc tacaaaggac ctaatgtata aggaaagaat atttagagaa aagaagaaaa 11821 caagagtttt atatacatac agagcacatg cctcgaggtc gaccccggga aaaagaacac 11881 cctgtaccag acaaaaagaa caccctgtac cagacgtcga caaaaagaac accctgtacc 11941 agacaaaaag aacaccctgt accagacaaa aagaacaccc tgtaccagac ggatctacta 12001 gtgcggccgc taagtaagta agacgtcgag ctctaagtaa gtaacggccg ccaccgcggt 12061 ggagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg 12121 tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt 12181 gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt 12241 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc 12301 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc 12361 gctcttattg accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat 12421 ttcacccaat tgtagatatg ctaactccag caatgagttg atgaatctcg gtgtgtattt 12481 tatgtcctca gaggacaaca cctgttgtaa tcgttcttcc acacggatcc caattcgccc 12541 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 12601 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 12661 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 12721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 12781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 12841 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 12901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 12961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 13021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 13081 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 13141 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac 13201 gcatctgtgc ggtatttcac accgcataga tcggcaagtg cac //