LOCUS Exported 925 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS 2XMyc-LRRK2-LRRs SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 925) TITLE The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation JOURNAL J Biol Chem. 2008 Jun 13;283(24):16906-14. PUBMED 18397888 REFERENCE 2 (bases 1 to 925) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..925 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 41..59 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" regulatory 91..100 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 100..129 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 145..174 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" protein_bind 193..217 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gtgaaccgtc agaatccgct agcgattacg ccaagctcga aattaaccct cactaaaggg 61 aacaaaagct ggagctccac cgcggtggcg gccgccatgg agcagaaact catctctgaa 121 gaggatctga gcccgggcgg atccgagcag aaactcatct ctgaagagga tctgctgcag 181 gaattcgata tcacaagttt gtacaaaaaa gcaggctccg aattcgccct tgactcttct 241 atggacagtg tgtttgctca aagtgatgac ctggatagtg aaggaagtga aggctcattt 301 cttgtgaaaa agaaatctaa ttcaattagt gtaggagaat tttaccgaga tgccgtatta 361 cagcgttgct caccaaattt gcaaagacat tccaattcct tggggcccat ttttgatcat 421 gaagatttac tgaagcgaaa aagaaaaata ttatcttcag atgattcact caggtcatca 481 aaacttcaat cccatatgag gcattcagac agcatttctt ctctggcttc tgagagagaa 541 tatattacat cactagacct ttcagcaaat gaactaagag atattgatgc cctaagccag 601 aaatgctgta taagtgttca tttggagcat cttgaaaagc tggagcttca ccagaatgca 661 ctcacgagct ttccacaaca gctatgtgaa actctgaaga gtttgacaca tttggacttg 721 cacagtaata aatttacatc atttccttct tatttgttga aaatgagttg tattgctaat 781 cttgatgtct ctcgaaatga cattggaccc tcagtggttt tagatcctac agtgaaatgt 841 ccaactctga aacagtttaa cctgtcatat aaccagctgt cttttgtacc tgagaacctc 901 actgatgtgg tagagaaact ggaca //