LOCUS       Exported               18827 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Targeting vector to Gt(ROSA)26 locus for conditional expression of 
            distinct FPs (Venus, mCherry, and mCerulean) responding to each 
            site-specific-recombinase activity (Cre, Dre, and phiC31o)..
ACCESSION   .
VERSION     .
KEYWORDS    ROSA26(MultiFPs-Delta-Puro)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata 
            S, Ikawa M, Okano H
  TITLE     Developmental analyses of mouse embryos and adults using a 
            non-overlapping tracing system for all three germ layers.
  JOURNAL   Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 
            10.1242/dev.174938.
  PUBMED    31597657
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        5303..5682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5684..5961
                     /label=chicken beta-actin promoter
     intron          5962..6978
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6902..6924
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6986..7005
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(7032..7065)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    7071..7104
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             7151..7750
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7151..7170)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7647..7667
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7757..7805
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(7817..7850)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      7856..7865
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7862..8581
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8584..8603)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8649..8704
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8703..8722)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      9074..9083
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9080..9790
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9225..9243)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9493..9512
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    9913..10034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      10085..10094
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             10091..10810
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10816..10833)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10822..11046
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(11070..11103)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    11111..12193
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    11741..12389
                     /label=HA-L
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    12195..13005
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    12519..13168
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(16444..16460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16444..16460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16457..16479)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16468..16484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(16492..16522)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16537..16558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        16617..16812
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      16663..16798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     16725..16744
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    16818..16952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(17020..17037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17191..17779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17271..17290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17950..18810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18573..18592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accggtcaac actgcctccc tgctgtgcaa agagcccttt gtcctgcccc gcacttatct
       61 agctgtttgg ccatattgac aggcctactg gaggcataat aagggagtca gtctgacata
      121 agcagctggc gatttcaaac aaaaatcttc cttttctact cacacaaaat cctgctgcag
      181 aggtctgtgg ctcctcccat acttccacct agttctcaaa tagagcagca aggaactggc
      241 atttcccctc gattccgtag tataaatcaa aaacttgatt ctctagccag aattcttata
      301 gccactgatt tgttgctgtt ttgtgttgta atccctaaat taggtttctt catctctggt
      361 atatacatgt atgattatca cttcaatgtg ccatgaaaac tctgggaagc ctcatactac
      421 aagacagaag agacatggaa gtggcaaaca cacacaccac aaacaaaact gagcaatact
      481 cttagggttt tgagaatacc aatgattttt aaaagattaa aaaaaaaaat cactttcctg
      541 acaccaaaga tactttatgg aaatttacat tcaggcattt ccctagatac tctgaaaaaa
      601 agaaagaaag aaagaaagaa aggaaggaag gaaggaagga aggaaggaag gaaggaagga
      661 aggaaacagt aatggaagga aggggttggg gtttgagaac ccttactcat aagaaaaaca
      721 tagactgtac ttttgtgtac ctcacagctc ctttcttcaa gtactgagta agaactcgtc
      781 caagggatgc tttctaacag aaccaactct cagggttact ggcatgtcta cccagtttat
      841 aacttaatac acaacaagac aatttcacta tacgtatcgt taagtgaaag aaaggctact
      901 ccttttacct tttgcaattt ccctgaattc cagccactag agaaacgaat cacaatacca
      961 aatgaaggtc atgccattta ctagatgtgt tagaatatct gggctccaag acttatctac
     1021 ctcataggtg cattgctctt cttttctaag tctatctgaa aggacacttt aaaccaaatt
     1081 ataaatgact accagtttaa aaaaaatgac taccagttga actattatac gcatattata
     1141 cgcatatttc agtctaagtt caccactagt caggcctcct aatttcaaat taaactgaaa
     1201 aaaaaaaaaa aaaactagtt tagccaaaag ttgtaataaa attttatttt acttatgatc
     1261 taatttattg tagatatgta ctcaataatt actgactact attgccttat gtttcaaagt
     1321 tccaccttca tcaaaagggt cagaataatt tctaaagtat acaggcttct agatactaag
     1381 cagtatcaag atgtaggcat ttttaagtgt gtcagttcaa gtaatccttc taagaaaaaa
     1441 gcctcttgat cttccagaga ttactgtaag accacaatca gatccaaaaa aacaggaaat
     1501 aaaagtggta gtcattattt tctaagggaa cttgtatatt ataattaagc tcaaaacact
     1561 tgcaccttaa ctgtaaatac cttgaactgt aatatcagta tgagtgatat catacttatg
     1621 ggatacagca cctaatctac gtaacaaaaa tattattaca accatctaaa acaatcatta
     1681 acagagtttt gttttatgga cttgctttta gcactggaaa aacaatatga actacatttg
     1741 tgaattcaaa atactctaca atgtttcttc attttttgct acaaattcta gaaccattac
     1801 atttatggga tctaaataaa tttgtacaaa attaaacaga ctcccgaagg aaaaggcatt
     1861 ggttgcagta ataatcctag ggaggaaaaa ccctacgatt ttatgtgctg cacatgcctg
     1921 ggtgatggtt cagtttcaaa tattccttgc cagacaaact acttaaaaag gcttaatcaa
     1981 agatcaccct ggttctattt ggttaggggt agggtgggct tagaatggac tcttgctgcc
     2041 ttcttagtat cttgaactaa acttactcaa tttctgaata ttccttctgt gtctctgatg
     2101 gattactgcc aaaacacatc tttgttcggt ggaaagtcta taatccaaca catacacgat
     2161 taatccaatt tataggtcag atatcagtaa gtatatctaa tctggagagg tctacagatt
     2221 aatttagaat taacaacaaa ccaagcatta atccaaaagc agctcttttt agtttaggca
     2281 tccaaatata tagtaagatg tttcaaagaa catcaatcta ttcacaaagg taaatacggc
     2341 ctaatacgga gagacaaata caaatctgaa atgactcaag caattaaaag atggaggaat
     2401 tcatgtcact tgcactggga aaacaaaaca aagatcctcc tcaccctatt acccacatta
     2461 ctatttttag gttttttagg ggttaaaaaa gatcaaaaca ctaatgaact ttaagtcctg
     2521 tgaagggtaa aacctcagat agtaacaaaa agcttccaac ccctcctcaa acaaaaaacc
     2581 ccaagtcttt aactttgatc cagttttcag atgctgatat ccataaatgg atacagttat
     2641 gaattgctaa ttctggtctc ttcactagca aaaagcaaag cagctcagca gtacaatttc
     2701 ccaggaaagc aagcaaggtt tctttccagc ctgagcagcc atcactaagt gcagttccct
     2761 gcagccaaca gcattaatgg acgctgcact gctgtccttc cctggagaca gcagccagca
     2821 ctactcaagc ttctcacgta gcaaccagag ctccagagcc agcagctgct gccgccttgt
     2881 atactcactc ctgtgatcca acacaggagc aaccttttct ttaccccacc cccacttctt
     2941 aacacacttt tttttggggg ggggggggaa caagtgctcc atgctggaag gattggaact
     3001 atgcttttag aaaggaacaa tcctaaggtc acttttaaat tgaggtcttt gatttgaaaa
     3061 tcaacaaata ccaaattcca aatattcgtt ttaattaaac cagcaatgtg gatataagca
     3121 ttaagtttta gttttaaaaa ggtcaatttt ccaaacattc agcaatcata tttaaattta
     3181 cagctaggaa caagagcctt gggtcatgtc ctaccaaaga acataactca atattctaca
     3241 catgacaatc tgaataacct taaagcctct aatcccataa caggccacaa attttggaca
     3301 gagaactaat gatcctcctg agaaaactgg aagaaatcca gggaaaagaa attcctgtgt
     3361 cctccaaact cagaaatctc taattatgtc agtattctct gctttagtcc taggtcagat
     3421 tgcacacatc taaaataacc tcttaaagtt ttcctcctag cgacctaaac cattattaat
     3481 atcaaattaa ccatcaaaac actttcctct caatatgctg cacacaaacc tcctcctgga
     3541 acctcctcca tctggatcct ccccaatcaa aagtataggt atttaacata taagcaagga
     3601 agtaatgtaa acatgacctt ggtcacaaat atgtcatcta aaaacaattt agtcaaggta
     3661 tggaggaaat tcgagaacct gaatcttttt aagtattttg agcacaggaa caattggcaa
     3721 aaggaatcca ggtatagaca aaacccagag cccagagctc tgggcgaaaa atgagttgct
     3781 ggtgaagacg ttacacaagt aacatgagaa agcagaaaat gcaggtcatc cacgcacccc
     3841 tgacccaggc cagcagggcg ggctgcagca tcagtacaca ggagaaagat ccttattcct
     3901 aagaatgaga aaggcaaagg cgcccgatag aataaattag catagaaggg gctttcccag
     3961 gagttaaaac tttccttctg agcgattacc tactaaaacc agggcttttg cccactacca
     4021 tttacctagg atcttggctt gcacggattc ataggggcat atccctcccc ctcttcttta
     4081 gagtcgttct taaaagatcg ctctccacgc cctaggcagg gaaaacgaca aaatctggct
     4141 caattccagg ctagaaccct acaaattcaa cagggatatc gcaaggatac tggggcatac
     4201 gccacaggga gtccaagaat gtgaggtggg ggtggcgaag gtaatgtctt tggtgtggga
     4261 aaagcagcag ccatctgaga taggaactgg aaaaccagag gagaggcgtt caggaagatt
     4321 atggagggga ggactgggcc cccacgagcg accagagttg tcacaaggcc gcaagaacag
     4381 gggaggtggg gggctcaggg acagaaaaaa aagtatgtgt attttgagag cagggttggg
     4441 aggcctctcc tgaaaagggt ataaacgtgg agtaggcaat acccaggcaa aaaggggaga
     4501 ccagagtagg gggaggggaa gagtcctgac ccagggaaga cattaaaaag gtagtggggt
     4561 cgactagatg aaggagagcc tttctctctg ggcaagagcg gtgcaatggt gtgtaaaggt
     4621 agctgagaag acgaaaaggg caagcatctt cctgctacca ggctggggag gcccaggccc
     4681 acgaccccga ggagagggaa cgcagggaga ctgaggtgac ccttctttcc cccggggccc
     4741 ggtcgtgtgg ttcggtgtct cttttctgtt ggacccttac cttgacccag gcgctgccgg
     4801 ggcctgggcc cgggctgcgg cgcacggcac tcccgggagg cagcgagact cgagttaggc
     4861 ccaacgcggc gccacggcgt ttcctggccg ggaatggccc gtacccgtga ggtgggggtg
     4921 gggggcagaa aaggcggagc gagcccgagg cggggrgggg gggggccagg ggcggrgggg
     4981 gccggcacta ctgtgttggc ggactggcgg gactagggct gcgtgagtct ctgagcgcag
     5041 gcgggcggcg gccgcccctc ccccggcggc ggcagcggcg gcagcggcgg cagctcactc
     5101 agcccgctgc ccgagcggaa acgccactga ccgcacgggg attcccagtg ccggcgccag
     5161 gggcacgcgg gacacgcccc ctcccgccgc gccattggcc tctccgccca ccgccccaca
     5221 cttattggcc ggtgcgccgc caatcagcgg aggctgccgg ggccgcctaa agaagaggct
     5281 gtgctttggg gctccctggg tcgacattga ttattgacta gttattaata gtaatcaatt
     5341 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     5401 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     5461 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     5521 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     5581 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     5641 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     5701 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     5761 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccrggsggg
     5821 gsgggggggg gsgrggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag
     5881 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
     5941 aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc
     6001 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
     6061 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
     6121 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
     6181 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
     6241 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
     6301 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
     6361 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
     6421 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
     6481 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
     6541 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaggggcgc
     6601 ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta
     6661 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa
     6721 tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc
     6781 aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct
     6841 ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
     6901 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
     6961 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     7021 ggcaaagaat tataacttcg tataatgtat gctatacgaa gttatcagag gtgccagggc
     7081 gtgcccttgg gctccccggg cgcggactaa ctttaaataa tgccaattat ttaaagttac
     7141 agccgccacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     7201 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     7261 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     7321 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     7381 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     7441 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     7501 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     7561 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     7621 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     7681 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     7741 cggtgcctga ggggatcggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
     7801 tgttcggatc actagtataa cttcgtataa tgtatgctat acgaagttat cagccgccac
     7861 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     7921 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     7981 cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac
     8041 cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     8101 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     8161 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     8221 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     8281 caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa
     8341 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc
     8401 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     8461 ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     8521 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     8581 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     8641 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     8701 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     8761 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     8821 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     8881 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     8941 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     9001 catagctgtc cctcttctct tatggagatc ggatcctaac tttaaataat gccaattatt
     9061 taaagttaca gccgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     9121 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     9181 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     9241 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     9301 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     9361 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     9421 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     9481 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     9541 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     9601 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     9661 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     9721 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     9781 gtacaagtga tgcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     9841 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     9901 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9961 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10021 atcaatgtat cttagaattc ccccaactgg ggtaaccttt gagttctctc agttgggggc
    10081 agccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
    10141 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
    10201 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
    10261 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga
    10321 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
    10381 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
    10441 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
    10501 cctggggcac aagctggagt acaacgccat cagcgacaac gtctatatca ccgccgacaa
    10561 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
    10621 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
    10681 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
    10741 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
    10801 gtacaagtga gatagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    10861 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    10921 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    10981 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    11041 ctatggcttc tgaggcggaa agaacctgcg aagttcctat actttctaga gaataggaac
    11101 ttcggcggcc gcggcaggcc ctccgagcgt ggtggagccg ttctgtgaga cagccgggta
    11161 cgagtcgtga cgctggaagg ggcaagcggg tggtgggcag gaatgcggtc cgccctgcag
    11221 caaccggagg gggagggaga agggagcgga aaagtctcca ccggacgcgg ccatggctcg
    11281 gggggggggg ngcagcggag gagcgcttcc ggccgacgtc tcgtcgctga ttggcttctt
    11341 ttcctcccgc cgtgtgtgaa aacacaaatg gcgtgttttg gttggcgtaa ggcgcctgtc
    11401 agttaacggc agccggagtg cgcagccgcc ggcagcctcg ctctgcccac tgggtggggc
    11461 gggaggtagg tggggtgagg cgagctggac gtgcgggcgc ggtcggcctc tggcggggcg
    11521 ggggrgggga gggagggtca gcgaaagtag ctcgcgcgcg agcggccgcc caccctcccc
    11581 ttcctctggg ggagtcgttt tacccgccgc cggccgggcc tcgtcgtctg attggctctc
    11641 ggggcccaga aaactggccc ttgccattgg ctcgtgttcg tgcaagttga gtccatccgc
    11701 cggccagcgg gggcggcgag gaggcgctcc caggttccgg ccctcccctc ggccccgcgc
    11761 cgcagagtct ggccgcgcgc ccctgcgcaa cgtggcagga agcgcgcgct gggggcgggg
    11821 acgggcagta gggctgagcg gctgcggggc gggtgcaagc acgtttccga cttgagttgc
    11881 ctcaagaggg gcgtgctgag ccagacctcc atcgcgcact ccggggagtg gagggaagga
    11941 gcgagggctc agttgggctg ttttggaggc aggaagcact tgctctccca aagtcgctct
    12001 gagttgttat cagtaaggga gctgcagtgg agtaggcggg gagaaggccg cacccttctc
    12061 cggagggggg aggggagtgt tgcaatacct ttctgggagt tctctgctgc ctcctggctt
    12121 ctgaggaccg ccctgggcct gggagaatcc cttccccctc ttccctcgtg atctgcaact
    12181 ccagtctttc tagaagatgg gcgggagtct tctgggcagg cttaaaggct aacctggtgt
    12241 gtgggcgttg tcctgcaggg gaattgaaca ggtgtaaaat tggagggaca agacttccca
    12301 cagattttcg gttttgtcgg gaagtttttt aataggggca aataaggaaa atgggaggat
    12361 aggtagtcat ctggggtttt atgcagcaaa actacaggtt attattgctt gtgatccgcc
    12421 tcggagtatt ttccatcgag gtagattaaa gacatgctca cccgagtttt atactctcct
    12481 gcttgagatc cttactacag tatgaaatta cagtgtcgcg agttagacta tgtaagcaga
    12541 attttaatca tttttaaaga gcccagtact tcatatccat ttctcccgct ccttctgcag
    12601 ccttatcaaa aggtatttta gaacactcat tttagcccca ttttcattta ttatactggc
    12661 ttatccaacc cctagacaga gcattggcat tttccctttc ctgatcttag aagtctgatg
    12721 actcatgaaa ccagacagat tagttacata caccacaaat cgaggctgta gctggggcct
    12781 caacactgca gttcttttat aactccttag tacacttttt gttgatcctt tgccttgatc
    12841 cttaattttc agtgtctatc acctctcccg tcaggtggtg ttccacattt gggcctattc
    12901 tcagtccagg gagttttaca acaatagatg tattgagaat ccaacctaaa gcttaacttt
    12961 ccactcccat gaatgcctct ctcctttttc tccatttata aactgagcta ttaaccatta
    13021 atggtttcca ggtggatgtc tcctccccca atattacctg atgtatctta catattgcca
    13081 ggctgatatt ttaagacatt aaaaggtata tttcattatt gagccacatg gtattgatta
    13141 ctgcttacta aaattttgtc attgtacaca tctgtaaaag gtggttcctt ttggaatgca
    13201 aagttcaggt gtttgttgtc tttcctgacc taaggtcttg tgagcttgta ttttttctat
    13261 ttaagcagtg ctttctcttg gactggcttg actcatggca ttctacacgt tattgctggt
    13321 ctaaatgtga ttttgccaag cttcttcagg acctataatt ttgcttgact tgtagccaaa
    13381 cacaagtaaa atgattaagc aacaaatgta tttgtgaagc ttggttttta ggttgttgtg
    13441 ttgtgtgtgc ttgtgctcta taataatact atccaggggc tggagaggtg gctcggagtt
    13501 caagagcaca gactgctctt ccagaagtcc tgagttcaat tcccagcaac cacatggtgg
    13561 ctcacaacca tctgtaatgg gatctgatgc cctcttctgg tgtgtctgaa gaccacaagt
    13621 gtattcacat taaataaata aatcctcctt cttcttcttt tttttttttt taaagagaat
    13681 actgtctcca gtagaattta ctgaagtaat gaaatacttt gtgtttgttc caatatggta
    13741 gccaataatc aaattactct ttaagcactg gaaatgttac caaggaacta atttttattt
    13801 gaagtgtaac tgtggacaga ggagccataa ctgcagactt gtgggataca gaagaccaat
    13861 gcagacttta atgtcttttc tcttacacta agcaataaag aaataaaaat tgaacttcta
    13921 gtatcctatt tgtttaaact gctagcttta cttaactttt gtgcttcatc tatacaaagc
    13981 tgaaagctaa gtctgcagcc attactaaac atgaaagcaa gtaatgataa ttttggattt
    14041 caaaaatgta gggccagagt ttagccagcc agtggtggtg cttgccttta tgcctttaat
    14101 cccagcactc tggaggcaga gacaggcaga tctctgagtt tgagcccagc ctggtctaca
    14161 catcaagttc tatctaggat agccaggaat acacacagaa accctgttgg ggaggggggc
    14221 tctgagattt cataaaatta taattgaagc attccctaat gagccactat ggatgtggct
    14281 aaatccgtct acctttctga tgagatttgg gtattatttt ttctgtctct gctgttggtt
    14341 gggtcttttg acactgtggg ctttctttaa agcctccttc ctgccatgtg gtctcttgtt
    14401 tgctactaac ttcccatggc ttaaatggca tggctttttg ccttctaagg gcagctgctg
    14461 agatttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact aaaattgtcc
    14521 tttaattttt tttttaaaaa atgggttata taataaacct cataaaatag ttatgaggag
    14581 tgaggtggac taatattaaa tgagtccctc ccctataaaa gagctattaa ggctttttgt
    14641 cttatactta actttttttt taaatgtggt atctttagaa ccaagggtct tagagtttta
    14701 gtatacagaa actgttgcat cgcttaatca gattttctag tttcaaatcc agagaatcca
    14761 aattcttcac agccaaagtc aaattaagaa tttctgactt ttaatgttaa tttgcttact
    14821 gtgaatataa aaatgatagc ttttcctgag gcagggtctc actatgtatc tctgcctgat
    14881 ctgcaacaag atatgtagac taaagttctg cctgcttttg tctcctgaat actaaggtta
    14941 aaatgtagta atacttttgg aacttgcagg tcagattctt ttatagggga cacactaagg
    15001 gagcttgggt gatagttggt aaaatgtgtt tcaagtgatg aaaacttgaa ttattatcac
    15061 cgcaacctac tttttaaaaa aaaaagccag gcctgttaga gcatgcttaa gggatcccta
    15121 ggacttgctg agcacacaag agtagttact tggcaggctc ctggtgagag catatttcaa
    15181 aaaacaaggc agacaaccaa gaaactacag ttaaggttac ctgtctttaa accatctgca
    15241 tatacacagg gatattaaaa tattccaaat aatatttcat tcaagttttc ccccatcaaa
    15301 ttgggacatg gatttctccg gtgaataggc agagttggaa actaaacaaa tgttggtttt
    15361 gtgatttgtg aaattgtttt caagtgatag ttaaagccca tgagatacag aacaaagctg
    15421 ctatttcgag gtctcttggt ttatactcag aagcacttct ttgggtttcc ctgcactatc
    15481 ctgatcatgt gctaggccta ccttaggctg attgttgttc aaataaactt aagtttcctg
    15541 tcaggtgatg tcatatgatt tcatatatca aggcaaaaca tgttatatat gttaaacatt
    15601 tgtacttaat gtgaaagtta ggtctttgtg ggtttgattt ttaattttca aaacctgagc
    15661 taaataagtc atttttacat gtcttacatt tggtggaatt gtataattgt ggtttgcagg
    15721 caagactctc tgacctagta accctaccta tagagcactt tgctgggtca caagtctagg
    15781 agtcaagcat ttcaccttga agttgagacg ttttgttagt gtatactagt ttatatgttg
    15841 gaggacatgt ttatccagaa gatattcagg actatttttg actgggctaa ggaattgatt
    15901 ctgattagca ctgttagtga gcattgagtg gcctttaggc ttgaattgga gtcacttgta
    15961 tatctcaaat aatgctggcc ttttttaaaa agcccttgtt ctttatcacc ctgttttcta
    16021 cataattttt gttcaaagaa atacttgttt ggatctcctt ttgacaacaa tagcatgttt
    16081 tcaagccata ttttttttcc tttttttttt tttttttggt ttttcgagac agggtttctc
    16141 tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc gaactcagaa
    16201 atccgcctgc ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc acgcctggct
    16261 aagttggata ttttgttata taactataac caatactaac tccactgggt ggatttttaa
    16321 ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac tgttcactct
    16381 aacagaggct gttggtacta gtggcactta agcaacttcc tacggaagct tggcgtaatc
    16441 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    16501 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    16561 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
    16621 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
    16681 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
    16741 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    16801 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    16861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    16921 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
    16981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    17041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    17101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    17161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    17221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    17281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    17341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    17401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    17461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    17521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    17581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    17641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    17701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    17761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    17821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    17881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    17941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    18001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    18061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    18121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    18181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    18241 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    18301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    18361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    18421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    18481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    18541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    18601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    18661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    18721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    18781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaat
//
