LOCUS Exported 7091 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCAG-Eco SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7091) TITLE Pseudotyping Envelopes JOURNAL Unpublished REFERENCE 2 (bases 1 to 7091) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7091 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 385..662 /label=chicken beta-actin promoter primer_bind 1603..1625 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" misc_feature 1666..2040 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1978..1994 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" primer_bind complement(4113..4132) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 4178..4233 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(4232..4251) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(4594..4610) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4594..4610) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4607..4629) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4618..4634 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4642..4672) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4687..4708 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4767..4962 /label=SV40 promoter /note="SV40 early promoter" rep_origin 4813..4948 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4875..4894 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 4968..5102 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5005..5024) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5059..5078 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5170..5187) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5341..5929) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5421..5440) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(6100..6960) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6723..6742 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6961..7065) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc 721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg 841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt 901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc 961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg 1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg 1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc 1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc 1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg 1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg 1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg 1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca 1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg 1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc 1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg 1621 tgaccggcgg ctctacgcaa cacgccgggc ccccatggcc tcaccccata tgagatctta 1681 tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac 1741 agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga 1801 cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga 1861 gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa 1921 ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct 1981 tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagactg 2041 acatggcgcg ttcaacgctc tcaaaacccc ttaaaaataa ggttaacccg cgaggccccc 2101 taatcccctt aattcttctg atgctcagag gggtcagtac tgcttcgccc ggctccagtc 2161 ctcatcaagt ctataatatc acctgggagg taaccaatgg agatcgggag acggtatggg 2221 caacttctgg caaccaccct ctgtggacct ggtggcctga ccttacccca gatttatgta 2281 tgttagccca ccatggacca tcttattggg ggctagaata tcaatcccct ttttcttctc 2341 ccccggggcc cccttgttgc tcagggggca gcagcccagg ctgttccaga gactgcgaag 2401 aacctttaac ctccctcacc cctcggtgca acactgcctg gaacagactc aagctagacc 2461 agacaactca taaatcaaat gagggatttt atgtttgccc cgggccccac cgcccccgag 2521 aatccaagtc atgtgggggt ccagactcct tctactgtgc ctattggggc tgtgagacaa 2581 ccggtagagc ttactggaag ccctcctcat catgggattt catcacagta aacaacaatc 2641 tcacctctga ccaggctgtc caggtatgca aagataataa gtggtgcaac cccttagtta 2701 ttcggtttac agacgccggg agacgggtta cttcctggac cacaggacat tactggggct 2761 tacgtttgta tgtctccgga caagatccag ggcttacatt tgggatccga ctcagatacc 2821 aaaatctagg accccgcgtc ccaatagggc caaaccccgt tctggcagac caacagccac 2881 tctccaagcc caaacctgtt aagtcgcctt cagtcaccaa accacccagt gggactcctc 2941 tctcccctac ccaacttcca ccggcgggaa cggaaaatag gctgctaaac ttagtagacg 3001 gagcctacca agccctcaac ctcaccagtc ctgacaaaac ccaagagtgc tggttgtgtc 3061 tagtagcggg acccccctac tacgaagggg ttgccgtcct gggtacctac tccaaccata 3121 cctctgctcc agccaactgc tccgtggcct cccaacacaa gttgaccctg tccgaagtga 3181 ccggacaggg actctgcata ggagcagttc ccaaaacaca tcaggcccta tgtaatacca 3241 cccagacaag cagtcgaggg tcctattatc tagttgcccc tacaggtacc atgtgggctt 3301 gtagtaccgg gcttactcca tgcatctcca ccaccatact gaaccttacc actgattatt 3361 gtgttcttgt cgaactctgg ccaagagtca cctatcattc ccccagctat gtttacggcc 3421 tgtttgagag atccaaccga cacaaaagag aaccggtgtc gttaaccctg gccctattat 3481 tgggtggact aaccatgggg ggaattgccg ctggaatagg aacagggact actgctctaa 3541 tggccactca gcaattccag cagctccaag ccgcagtaca ggatgatctc agggaggttg 3601 aaaaatcaat ctctaaccta gaaaagtctc tcacttccct gtctgaagtt gtcctacaga 3661 atcgaagggg cctagacttg ttatttctaa aagaaggagg gctgtgtgct gctctaaaag 3721 aagaatgttg cttctatgcg gaccacacag gactagtgag agacagcatg gccaaattga 3781 gagagaggct taatcagaga cagaaactgt ttgagtcaac tcaaggatgg tttgagggac 3841 tgtttaacag atccccttgg tttaccacct tgatatctac cattatggga cccctcattg 3901 tactcctaat gattttgctc ttcggaccct gcattcttaa tcgattagtc caatttgtta 3961 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc 4021 ctatagagta cgagccatag ttctgagcgc cggtcgctac cattaccagt tggtctggtg 4081 tcaaaaataa taataaccgg gcaaacggca gatctttttc cctctgccaa aaattatggg 4141 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt 4201 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat 4261 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg 4321 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct 4381 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt 4441 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga 4501 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc 4561 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 4741 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc 4801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 4861 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 4921 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg 4981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 5101 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 5161 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5221 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5281 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5341 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5401 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 5461 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 5521 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 5581 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 5641 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 5701 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 5761 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 5821 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 5881 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5941 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6001 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6061 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6121 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6181 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6241 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6301 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6361 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6421 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6481 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6541 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6601 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6661 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6721 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6781 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6841 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6901 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6961 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7021 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7081 agtgccacct g //