LOCUS       Exported                6474 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Localization: Centrosomes, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    mPlum-PLK1-N-16
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6474)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        59..362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        363..566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     517..537
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2458..3138
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     2859..2878
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3053..3076
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3260..3381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3297..3316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3351..3370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3388..3843)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3525..3546)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3737..3756
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3870..3974
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3976..4333
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3999..4019)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4184..4319
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4246..4265
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4368..5162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4422..4441)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5032..5051
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5350..5369)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5394..5441
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5770..6358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6259..6278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
       61 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      121 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      181 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      241 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      301 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      361 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      421 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      481 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      541 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgccacc
      601 atgagtgctg cagtgactgc agggaagctg gcacgggcac cggccgaccc tgggaaagcc
      661 ggggtccccg gagttgcagc tcccggagct ccggcggcgg ctccaccggc gaaagagatc
      721 ccggaggtcc tagtggaccc acgcagccgg cggcgctatg tgcggggccg ctttttgggc
      781 aagggcggct ttgccaagtg cttcgagatc tcggacgcgg acaccaagga ggtgttcgcg
      841 ggcaagattg tgcctaagtc tctgctgctc aagccgcacc agagggagaa gatgtccatg
      901 gaaatatcca ttcaccgcag cctcgcccac cagcacgtcg taggattcca cggctttttc
      961 gaggacaacg acttcgtgtt cgtggtgttg gagctctgcc gccggaggtc tctcctggag
     1021 ctgcacaaga ggaggaaagc cctgactgag cctgaggccc gatactacct acggcaaatt
     1081 gtgcttggct gccagtacct gcaccgaaac cgagttattc atcgagacct caagctgggc
     1141 aaccttttcc tgaatgaaga tctggaggtg aaaatagggg attttggact ggcaaccaaa
     1201 gtcgaatatg acggggagag gaagaagacc ctgtgtggga ctcctaatta catagctccc
     1261 gaggtgctga gcaagaaagg gcacagtttc gaggtggatg tgtggtccat tgggtgtatc
     1321 atgtatacct tgttagtggg caaaccacct tttgagactt cttgcctaaa agagacctac
     1381 ctccggatca agaagaatga atacagtatt cccaagcaca tcaaccccgt ggccgcctcc
     1441 ctcatccaga agatgcttca gacagatccc actgcccgcc caaccattaa cgagctgctt
     1501 aatgacgagt tctttacttc tggctatatc cctgcccgtc tccccatcac ctgcctgacc
     1561 attccaccaa ggttttcgat tgctcccagc agcctggacc ccagcaaccg gaagcccctc
     1621 acagtcctca ataaaggctt ggagaacccc ctgcctgagc gtccccggga aaaagaagaa
     1681 ccagtggttc gagagacagg tgaggtggtc gactgccacc tcagtgacat gctgcagcag
     1741 ctgcacagtg tcaatgcctc caagccctcg gagcgtgggc tggtcaggca agaggaggct
     1801 gaggatcctg cctgcatccc catcttctgg gtcagcaagt gggtggacta ttcggacaag
     1861 tacggccttg ggtatcagct ctgtgataac agcgtggggg tgctcttcaa tgactcaaca
     1921 cgcctcatcc tctacaatga tggtgacagc ctgcagtaca tagagcgtga cggcactgag
     1981 tcctacctca ccgtgagttc ccatcccaac tccttgatga agaagatcac cctccttaaa
     2041 tatttccgca attacatgag cgagcacttg ctgaaggcag gtgccaacat cacgccgcgc
     2101 gaaggtgatg agctcgcccg gctgccctac ctacggacct ggttccgcac ccgcagcgcc
     2161 atcatcctgc acctcagcaa cggcagcgtg cagatcaact tcttccagga tcacaccaag
     2221 ctcatcttgt gcccactgat ggcagccgtg acctacatcg acgagaagcg ggacttccgc
     2281 acataccgcc tgagtctcct ggaggagtac ggctgctgca aggagctggc cagccggctc
     2341 cgctacgccc gcactatggt ggacaagctg ctgagctcac gctcggccag caaccgtctc
     2401 aaggcctccg gtagcggcag cgcatccggc ggaagcggaa gcggaccggt ggctaccatg
     2461 gtgagcaagg gcgaggaggt catcaaggag ttcatgcgct tcaaggagca catggagggc
     2521 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2581 acccagaccg ccaggctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2641 ctgtcccctc agatcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2701 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2761 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2821 aaggtgaagg tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     2881 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2941 atgaagatga ggctgaggct gaaggacggc ggccactacg acgccgaggt caagaccacc
     3001 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
     3061 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
     3121 cactccaccg gcgcctaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     3181 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     3241 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     3301 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     3361 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     3421 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3481 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3541 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3601 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3661 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3721 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3781 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3841 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3901 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3961 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     4021 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4081 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4141 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4201 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4261 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4321 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4381 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4441 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4501 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     4561 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4621 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4681 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4741 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4801 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4861 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     4921 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4981 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5041 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5101 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5161 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5221 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5281 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     5341 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     5401 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     5461 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     5521 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     5581 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     5641 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5701 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5881 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc atta
//
