LOCUS Exported 7303 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Expression of AuroraA kinase biosensor under CMV promoter. ACCESSION . VERSION . KEYWORDS pCMV superYFP-AURKA-mTurq2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7303) AUTHORS Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier M TITLE Aurora kinase A localises to mitochondria to control organelle dynamics and energy production. JOURNAL Elife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111. PUBMED 30070631 REFERENCE 2 (bases 1 to 7303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7303 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..204 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 155..175 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 282..941 /codon_start=1 /product="tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3" /label=LanYFP /translation="MASLPATHELHIFGSFNGVDFDMVGRGTGNPNDGYEELNLKSTKG DLQFSPWILVPQIGYGFHQYLPFPDGMSPFQAAMKDGSGYQVHRTMQFEDGASLTSNYR YTYEGSHIKGEFQVKGTGFPADGPVMTNSLTAADWCVTKMLYPNDKTIISTFDWTYTTG NGKRYQSTARTTYTFAKPMAANILKNQPMFVFRKTELKHSKTELNFKEWQKAFTDVM" CDS 993..1652 /codon_start=1 /product="tetrameric yellow fluorescent protein from Branchiostoma lanceolatum, also known as blFP-Y3" /label=LanYFP /translation="MASLPATHELHIFGSFNGVDFDMVGRGTGNPNDGYEELNLKSTKG DLQFSPWILVPQIGYGFHQYLPFPDGMSPFQAAMKDGSGYQVHRTMQFEDGASLTSNYR YTYEGSHIKGEFQVKGTGFPADGPVMTNSLTAADWCVTKMLYPNDKTIISTFDWTYTTG NGKRYQSTARTTYTFAKPMAANILKNQPMFVFRKTELKHSKTELNFKEWQKAFTDVM" CDS 2589..2600 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2964..2985) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3225..3244) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3572..3593 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 3727..3848 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3764..3783) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3818..3837 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3855..4310) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3992..4013) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4204..4223 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4337..4441 /gene="bla" /label=AmpR promoter promoter 4443..4800 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(4466..4486) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4651..4786 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4713..4732 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4835..5629 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4889..4908) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5499..5518 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5817..5836) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5861..5908 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 6237..6825 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6726..6745 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 7000..7303 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg 241 actcagatct cgagatggtc tccaaaggag aggaggataa catggcctct ctcccagcga 301 cacatgagtt acacatcttt ggctccttca acggtgtgga ctttgacatg gtgggtcgtg 361 gcaccggcaa tccaaatgat ggttatgagg agttaaacct gaagtccacc aagggtgacc 421 tccagttctc cccctggatt ctggtccctc aaatcgggta tggattccat cagtacctgc 481 ccttccccga cgggatgtcg cctttccagg ccgccatgaa agatggctcc ggataccaag 541 tccatcgcac aatgcagttt gaagacggtg cctccctgac ttccaactac cgctacacct 601 acgagggaag ccacatcaaa ggagagtttc aggtgaaggg gactggtttc cctgctgacg 661 gtcctgtgat gaccaactcg ctgaccgctg cggactggtg cgtgaccaag atgctgtacc 721 ccaacgacaa aaccatcatc agcacctttg actggactta caccactgga aatggcaagc 781 gctaccagag cactgcgcgg accacctaca cctttgccaa gccaatggcg gccaacatcc 841 tgaagaacca gccgatgttc gtgttccgta agacggaact caagcactcc aagaccgaac 901 tcaacttcaa ggagtggcaa aaggcattta ccgatgtgat gtcgactggc actggttcta 961 cgggctcggg ctcctcagga gaggaggata acatggcctc tctcccagcg acacatgagt 1021 tacacatctt tggctccttc aacggtgtgg actttgacat ggtgggtcgt ggcaccggca 1081 atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac ctccagttct 1141 ccccctggat tctggtccct caaatcgggt atggcttcca tcagtacctg cccttccccg 1201 acgggatgtc gcctttccag gccgccatga aagatggctc cggataccaa gtccatcgca 1261 caatgcagtt tgaagacggt gcctccctga cttccaacta ccgctacacc tacgagggaa 1321 gccacatcaa aggagagttt caggtgaagg ggactggttt ccctgctgac ggtcctgtga 1381 tgaccaactc gctgaccgct gcggactggt gcgtgaccaa gatgctgtac cccaacgaca 1441 aaaccatcat cagcaccttt gactggactt acaccactgg aaatggcaag cgctaccaga 1501 gcactgcgcg gaccacctac acctttgcca agccaatggc ggccaacatc ctgaagaacc 1561 agccgatgtt cgtgttccgt aagacggaac tcaagcactc caagaccgaa ctcaacttca 1621 aggagtggca aaaggcattt accgatgtga tgggcatgga cgagctgtac aagggaagct 1681 tcgaattcat ggaccgatct aaagaaaact gcatttcagg acctgttaag gctacagctc 1741 cagttggagg tccaaaacgt gttctcgtga ctcagcaatt tccttgtcag aatccattac 1801 ctgtaaatag tggccaggct cagcgggtct tgtgtccttc aaattcttcc cagcgcattc 1861 ctttgcaagc acaaaaactt gtctccagtc acaagccggt tcagaatcag aagcagaagc 1921 aattgcaggc aaccagtgta cctcatcctg tctccaggcc actgaataac acccaaaaga 1981 gcaagcagcc cctgccatcg gcacctgaaa ataatcctga ggaggaactg gcatcaaaac 2041 agaaaaatga agaatcaaaa aagaggcagt gggctttgga agactttgaa attggtcgcc 2101 ctctgggtaa aggaaagttt ggtaatgttt atttggcaag agaaaagcaa agcaagttta 2161 ttctggctct taaagtgtta tttaaagctc agctggagaa agccggagtg gagcatcagc 2221 tcagaagaga agtagaaata cagtcccacc ttcggcatcc taatattctt agactgtatg 2281 gttatttcca tgatgctacc agagtctacc taattctgga atatgcacca cttggaacag 2341 tttatagaga acttcagaaa ctttcaaagt ttgatgagca gagaactgct acttatataa 2401 cagaattggc aaatgcacta tcatattgcc attcgaagag agttattcat agagacatta 2461 agccagagaa cttacttctt ggatcagctg gagagcttaa aattgcagat tttgggtggt 2521 cagtacatgc tccatcttcc aggaggacca ctctctgtgg caccctggac tacctgcccc 2581 ctgaaatgat tgaaggtcgg atgcatgatg agaaggtgga tctctggagc cttggagttc 2641 tttgctatga atttttagtt gggaagcctc cttttgaggc aaacacatac caagagacct 2701 acaaaagaat atcacgggtt gagttcacat tccctgactt tgtaacagag ggagccaggg 2761 acctcatttc aagactgttg aagcataatc ccagccagag gccaatgctc agagaagtac 2821 ttgaacaccc ctggatcaca gcaaattcat caaaaccatc aaattgccaa aacaaagaat 2881 cagctagcaa acagtctggg gatccgatgg cggccgcaat ggtgagcaag ggcgaggagc 2941 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 3001 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 3061 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgtcctggg 3121 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 3181 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 3241 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 3301 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacttta 3361 gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga 3421 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc 3481 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc 3541 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 3601 cctaagcggc cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt 3661 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 3721 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3781 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 3841 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 3901 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 3961 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 4021 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 4081 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 4141 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 4201 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 4261 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 4321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 4381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 4441 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 4501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 4561 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4621 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 4681 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 4741 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 4801 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 4861 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4921 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4981 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 5041 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 5101 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 5161 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 5221 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5281 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5341 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 5401 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5461 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 5521 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 5581 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 5641 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 5701 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 5761 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 5821 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 5881 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 5941 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 6001 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 6061 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 6121 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 6181 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 6241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 6301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 6361 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 6421 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 6481 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6541 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 6601 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6661 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 6721 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 6781 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 6841 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 6901 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat 6961 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 7021 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 7081 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 7141 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt 7201 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc 7261 tacttggcag tacatctacg tattagtcat cgctattacc atg //