LOCUS       Exported               12707 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA 
            cloning using GoldenGate.
ACCESSION   .
VERSION     .
KEYWORDS    JME5000
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12707)
  AUTHORS   Larroude M, Trabelsi H, Nicaud JM, Rossignol T
  TITLE     A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting 
            wild-type strain diversity.
  JOURNAL   Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 
            10.1007/s10529-020-02805-4.
  PUBMED    31974649
REFERENCE   2  (bases 1 to 12707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      12..69
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(93..110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1023..1883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1646..1665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1884..1988)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2078..2121
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    2700..2721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2736..2766
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2774..2790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2808..2819
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2826..3503
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3548..3619
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3635..3662
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(6326..6348)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6448..6467
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6661..6683
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6675..6692
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6676..6692
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8299..12402
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12415..12435
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      12446..12693
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(12462..12480)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
       61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
      781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
      841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
      901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
      961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2101 gctttcgcta aggatgattt ctggaattcg aagagcctct gacctgacgt ccccagttgc
     2161 aaaagttgac acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg
     2221 tgattatata atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac
     2281 gctctaatgc ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt
     2341 gtatgagact ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta
     2401 aacaaagttt tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt
     2461 gtgcgttcga gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga
     2521 tggtttagtg gtaaaatcca tcgttgccat cgatgggccc ccggttcgag agacggggcc
     2581 ggccatctgg gccctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
     2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
     2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
     2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
     3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
     3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
     3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
     3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     3661 cgtttatata ggccatctgg gccgggggcg tctctgagct agaaatagca agttaaaata
     3721 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta
     3781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc
     3841 ctctagagtc gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg
     3901 tgagtgttga gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga
     3961 atatacagta gtatgcggcc gctaggtctg ttatccctag atcgatatag agactgggtt
     4021 ggcggcgcat ttgtgtccca aaaaacagcc ccaattgccc caattgaccc caaattgacc
     4081 cagtagcgga cccaaccccg gcgagagccc ccttcacccc acatatcaaa cctcccccgg
     4141 ttcccacact tgccgttaag ggcgtagggt actgcagtct ggaatctacg cttgttcaga
     4201 ctttgtacta gtttctttgt ctggccatcc gggtaaccca tgccggacgc aaaatagact
     4261 actgaaaatt tttttgcttt gtggttggga ctttagccaa gggtataaaa gaccaccgtc
     4321 cccgaattac ctttcctctt cttttctctc tctccttgtc aactcacacc cgaaggatcg
     4381 gatccacaat gtccacaaaa catctgttca acgagactga cgagctcgtg ctcaagtctc
     4441 tcgagggagt tcaggcttcc cgatcagcgt ccattctctc ccacagattc aaggtgctct
     4501 acaacggaac ccattccgct gaccgagttg cagttctttc tggaggaggc tctggccacg
     4561 agcctgccca cgccggcttt gtgggagaca acatgctgac cggtgccatc tgtggccccg
     4621 tcttcgcgtc cccttcagcc aagcaggtcg aggccggctg caagctcgtg ccctccgaca
     4681 agggtcatat tctggtcgtc accaactaca ccggcgacat gttgcatttt ggcctcgcgg
     4741 ccgagaagct caagtctcag ggccacaagg tcggcatcat taagtctgcc gacgatgtgg
     4801 ctgtcgaccg aaagagcggt ggtctggtcg gtcgacgagg actggctgga actgtgcttc
     4861 tggacaagat tgtcggagga gctgcctggg acaagctgtc ctttgatgaa tgcatggcca
     4921 ttggaaccga ggtggccgag aacaccgcaa ccgcctcaat tggactggac tactgccacg
     4981 ttcctggtcg atccgttgag aaccacgtgt cgctcgacca gaacgagtgt caattcggcc
     5041 tgggtattca caatgagccc ggagtcaaga ccatcaaccc cgtgcctgcc cctgagtcca
     5101 tggttgatac tctgctcaag tacctagttt cgcaggacga ccccgagcga tccttcgtaa
     5161 agttcaagga gggcgacgag gtcattctcc tggccaacaa cctcggtgga atttccacca
     5221 ttgagatgcg ggctgctgtc cagcttgccc gagagcaact cgagaagacc cacaagatca
     5281 agtctgtgcg agttctgtgc ggcaccttca tgagctctct caacgctccc ggcttctcta
     5341 tcaccctggt gaacctgtct aacggctccc acagcaagaa cgtgctcaag tacctggacg
     5401 ctgtgtccga cgcccctgcc tgggtcaatg tggcccctcc tacctcggtc aagcccttta
     5461 tcaacgagga caagatcttc gacgacgaga cttccaacat taaggctccc acactggaca
     5521 ttcctgaaca gaccgtggtt gctgctctca ctcaggcctc ccagaacatt atcaaggccg
     5581 agcctcagct tactgcatgg gataccgaaa tgggcgacgg agactgtgga cacaccattg
     5641 aacatggctg ccgagctctt ctcgagtacc tcaacaagaa caagagcgac ccaaaggctc
     5701 tcgagatcat ccccattgtg cgggctgtcg ttcacatcac cgaggaggat atgggaggca
     5761 ccctgggagc tatcttcggc attttttttg cctctttcct taacgctctt ctgctggacc
     5821 ctctgtccca taagactgat gtcaatgtga ctgataagct cgtcaacgct gctaacaccg
     5881 gtctggaaag tctcatgaac cacactcctg ctcgacctgg tgaccgaact gtcatggacg
     5941 tgttgatccc ctacgtgcag tctctggtgt ccaccaagga tatcaaggag gccgctctca
     6001 aggccaagca ggctgccgag ggcaccaaga agatcaagcc tcgtcttgga cgagcagtct
     6061 atgttgggga gaaggacggt gagcttcctc ccgaccctgg agcttgggct gtctacgagc
     6121 tggttgatgg ttttgccaac cacaagtagc ctagggtgtc tgtggtatct aagctattta
     6181 tcactcttta caacttctac ctcaactatc tactttaata aatgaatatc gtttattctc
     6241 tatgattact gtatatgcgt tcctctaaga caaatcgaat gacggggccc tttcgtcacg
     6301 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggtg acggtcacag
     6361 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     6421 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6481 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     6541 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     6601 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     6661 cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtacc cgcttttcgt
     6721 agataatgga atacaaatgg atatccagag tatacacatg gatagtatac actgacacga
     6781 caattctgta tctctttatg ttaactactg tgaggcgtta aatagagctt gatatataaa
     6841 atgttacatt tcacagtctg aacttttgca gattacctaa tttggtaaga tattaattat
     6901 gaactgaaag ttgatggcat ccctaaattt gatgaaaggg ggaacccccg ggttcgaagg
     6961 taccaaggaa gcatgcggta cccgaattcg cttcgatcgc gtatcgatga tacgcgtcca
     7021 tggtgaggtg tctcacaagt gccgtgcagt cccgccccca cttgcttctc tttgtgtgta
     7081 gtgtacgtac attatcgaga gggttgttcc cgcccacctc gatccggcat gctgaggtgt
     7141 ctcacaagtg ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag tgtacgtaca
     7201 ttatcgagag ggttgttccc gcccacctcg atccgacatg ytgaggtgtc tcacaagtgc
     7261 cgtgcagtcc cgcccccact tgcttctctt tgtgtgtagt gtacgtacat tatcgagagg
     7321 gttgttcccg cccacctcga tccggcatgc tgaggtgtct cacaagtgcc gtgcagtccc
     7381 gcccccactt gcttctcttt gtgtgtagtg tacgtacatt atcgagaggg ttgttcccgc
     7441 ccacctcgat ccggcatgct gaggtgtctc acaagtgccg tgcagtcccg cccccacttg
     7501 cttctctttg tgtgtagtgt acgtacatta tcgagagggt tgttcccgcc cacctcgatc
     7561 cggcatgctg aggtgtctca caagtgccgt gcagtcccgc ccccacttgc ttctctttgt
     7621 gtgtagtgta cgtacattat cgagagggtt gttcccgccc acctcgatcc ggcatgctga
     7681 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac
     7741 gtacattatc gagagggttg ttcccgccca cctcgatccg gcatgctgag gtgtctcaca
     7801 agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg tacattatcg
     7861 agagggttgt tcccgcccac ctcgatccgg catgcactga tcacgggcaa aagtgcttcg
     7921 atagagaggg ggttggcggc gcatttgtgt cccaaaaaac agccccaatt gccccaattg
     7981 accccaaatt gacccagtag cggacccaac cccggcgaga gcccccttca ccccacatat
     8041 caaacctccc ccggttccca cacttgccgt taagggcgta gggtactgca gtctggaatc
     8101 tacgcttgtt cagactttgt actagtttct ttgtctggcc atccgggtaa cccatgccgg
     8161 acgcaaaata gactactgaa aatttttttg ctttgtggtt gggactttag ccaagggtat
     8221 aaaagaccac cgtccccgaa ttacctttcc tcttcttttc tctctctcct tgtcaactca
     8281 cacccgaaga caaccacaat ggataagaaa tactccattg gcctggacat cggaaccaac
     8341 tccgtgggtt gggccgtgat caccgatgag tacaaggtgc cctctaagaa attcaaggtc
     8401 ctgggcaaca ccgaccgaca ctccatcaag aagaacctga tcggcgctct gctcttcgac
     8461 tctggcgaga ctgctgaggc cacccgactg aagcgaaccg ctcgaagacg atacacccga
     8521 agaaagaacc gaatctgtta cctgcaggag atcttctcta acgagatggc caaggtggac
     8581 gactctttct tccaccgact ggaggagtct ttcctggtgg aggaggacaa gaagcacgag
     8641 cgacacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc
     8701 atctaccacc tgcgaaagaa gctggtggac tctaccgaca aggccgacct gcgactgatc
     8761 tacctggccc tggcccacat gatcaagttc cgaggccact tcctgatcga gggcgacctg
     8821 aaccccgaca actctgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag
     8881 ctcttcgaag agaaccccat taacgcttct ggcgtggatg ctaaggccat cctgtctgcc
     8941 cgactgtcta agtctcgacg actcgagaac ctgattgctc agctccccgg agagaagaag
     9001 aacggtctgt tcggaaacct gattgctctg tccctgggtc tgacccctaa cttcaagtcc
     9061 aacttcgatc tggctgagga cgctaagctg cagctgtcta aggacaccta cgacgatgac
     9121 ctggataacc tgctcgccca gattggcgac cagtacgccg acctgttcct ggccgccaag
     9181 aacctgtctg acgccatcct gctgtctgac atcctgcgag tgaacaccga gatcaccaag
     9241 gcccccctgt ctgcctccat gattaagcga tacgatgagc accaccagga tctgaccctc
     9301 ctcaaggctc tggtccgaca gcagctgccc gagaagtaca aggagatttt cttcgaccag
     9361 tctaagaacg gctacgccgg ctacatcgac ggcggcgcct ctcaggagga gttctacaag
     9421 ttcattaagc ccatcctgga gaagatggac ggaaccgagg aactgctcgt gaagctgaac
     9481 cgagaggacc tcctgcgaaa gcagcgaacc ttcgacaacg gctctatccc ccaccagatc
     9541 cacctgggcg agctgcacgc catcctgcga cgacaggagg acttctaccc cttcctgaag
     9601 gacaaccgag agaagatcga gaagatcctg accttccgaa tcccctacta cgtgggaccc
     9661 ctggcccgag gaaactctcg attcgcttgg atgacccgaa agtctgagga gactattacc
     9721 ccctggaact tcgaggaggt ggtggataag ggcgcctctg ctcagtcttt catcgagcga
     9781 atgaccaact tcgacaagaa cctccccaac gagaaggtcc tgcccaagca ctctctgctc
     9841 tacgagtact tcaccgtcta caacgagctc accaaggtca agtacgtgac cgagggaatg
     9901 cgaaagcccg ctttcctgtc tggagagcag aagaaggcta ttgtggatct gctcttcaag
     9961 actaaccgaa aggtcaccgt caagcagctg aaggaggatt acttcaagaa gattgagtgt
    10021 ttcgattctg tcgagatctc cggcgtcgag gaccgattca acgcctctct gggtacctac
    10081 cacgacctgc tgaagattat caaggacaag gatttcctgg ataacgagga gaacgaggat
    10141 attctcgagg acattgtcct gaccctcacc ctgttcgagg atcgagagat gattgaggag
    10201 cgactcaaga cctacgctca cctgttcgac gacaaggtga tgaagcagct gaagcgacga
    10261 cgatacaccg gctggggccg actgtctcga aagctgatca acggcatccg agacaagcag
    10321 tctggcaaga ccatcctgga cttcctgaag tctgacggct tcgccaaccg aaacttcatg
    10381 cagctgatcc acgacgactc tctgaccttc aaggaggaca tccagaaggc ccaggtgtct
    10441 ggccagggcg actctctgca cgagcacatc gccaacctgg ccggctctcc cgccattaag
    10501 aaaggtatcc tgcagaccgt caaggtggtc gatgagctcg tcaaggtgat gggccgacac
    10561 aagcccgaga acattgtcat tgagatggct cgagagaacc agactactca gaagggtcag
    10621 aaaaactccc gagagcgaat gaagcgaatt gaggaaggta ttaaggagct gggctcccag
    10681 attctcaagg agcatcccgt ggagaacact cagctccaga acgagaagct gtacctgtac
    10741 tatctgcaga acggtcgaga catgtacgtc gaccaggagc tggatatcaa ccgactctcc
    10801 gactacgatg tggaccacat tgtgccccag tccttcctga aggacgattc tatcgataac
    10861 aaggtgctga cccgatccga caagaaccga ggcaagtctg acaacgtgcc ctccgaggag
    10921 gtggtcaaga agatgaagaa ctactggcga cagctgctga acgccaagct gattacccag
    10981 cgaaagttcg acaacctgac caaggccgag cgaggcggcc tgtctgagct ggacaaggcc
    11041 ggcttcatca agcgacagct ggtggagact cgacagatca ccaagcacgt ggcccagatc
    11101 ctggactctc gaatgaacac caagtacgac gagaacgaca agctgatccg agaggtgaag
    11161 gtgatcaccc tgaagtctaa gctggtgtct gacttccgaa aggacttcca gttctacaag
    11221 gtgcgagaga ttaacaacta ccaccacgcc cacgatgcct acctgaacgc tgtcgtgggc
    11281 accgccctca tcaagaagta tcccaagctg gagtccgagt tcgtctacgg cgactacaag
    11341 gtctacgatg tgcgaaaaat gattgccaag tccgagcagg agattggcaa ggctaccgcc
    11401 aagtacttct tctactccaa cattatgaac ttcttcaaga ccgagattac cctggctaac
    11461 ggcgagattc gaaagcgacc cctcattgag actaacggag agactggtga gatcgtgtgg
    11521 gacaagggac gagacttcgc caccgtgcga aaggtgctgt ctatgcccca ggtgaacatc
    11581 gtgaagaaga ccgaggtgca gaccggaggt ttctctaagg agtccatcct gcccaagcga
    11641 aactctgaca agctgatcgc ccgaaagaag gactgggacc ccaagaagta cggaggtttc
    11701 gactctccca ccgtggctta ctctgtgctg gtggtggcca aggtggagaa gggcaagtct
    11761 aagaagctga agtctgtgaa ggagctgctg ggcattacca tcatggagcg atcttctttc
    11821 gagaagaacc ccattgactt cctggaggcc aagggataca aggaggtgaa gaaagatctg
    11881 attatcaagc tccccaagta ctctctgttc gagctggaga acggacgaaa gcgaatgctg
    11941 gcctctgccg gcgagctgca gaagggaaac gagctggccc tgccctccaa gtacgtcaac
    12001 ttcctgtacc tcgcctccca ttacgagaag ctgaagggct ctcccgagga taacgagcag
    12061 aagcagctct tcgtggagca gcataagcac tacctggacg agatcatcga gcagatttct
    12121 gagttctcta agcgagtgat cctggctgac gccaacctgg ataaggtgct gtctgcttac
    12181 aacaagcacc gagacaagcc cattcgagag caggctgaga acatcattca cctgttcacc
    12241 ctgaccaacc tgggagcccc cgctgccttc aagtacttcg acaccaccat cgaccgaaag
    12301 cgatacacct ctaccaagga ggtgctggac gccaccctga tccaccagtc tatcaccggc
    12361 ctgtacgaga ctcgaatcga cctgtctcag ctgggcggcg actctcgagc cgaccccaag
    12421 aagaagcgaa aggtgtaagc tagcctcatg taattagtta tgtcacgctt acattcacgc
    12481 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
    12541 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
    12601 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
    12661 aaggttttgg gacgctcgaa ggctttaatt tgcaagcttg gcggtct
//
