LOCUS Exported 13986 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes. ACCESSION . VERSION . KEYWORDS At2S3:RUBY SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13986) AUTHORS He Y, Zhang T, Sun H, Zhan H, Zhao Y TITLE A reporter for noninvasively monitoring gene expression and plant transformation. JOURNAL Hortic Res. 2020 Sep 19;7:152. doi: 10.1038/s41438-020-00390-1. eCollection 2020. PUBMED 33024566 REFERENCE 2 (bases 1 to 13986) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13986 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 1..25 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 1975..2031 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 2866..2922 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" promoter 5128..5308 /label=NOS promoter /note="nopaline synthase promoter" promoter 5161..5344 /function="NOS promoter" /label=NOS promoter /note="nopaline synthase promoter" CDS 5359..6384 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" terminator 6386..6638 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(7214..7231) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7214..7230) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7223..7245) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 7452..7476 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 8004..8795 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 9039..9627 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9528..9547 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 9781..9798 /label=L4440 /note="L4440 vector, forward primer" misc_feature 9813..9953 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(9879..9898) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10039..10061 /label=pGEX 3' /note="pGEX vectors, reverse primer" rep_origin 10297..10491 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(10557..11624) /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" CDS complement(12058..12687) /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" ORIGIN 1 gtttacccgc caatatatcc tgtcaaacac tgatagtttt aagctggcac aactatattt 61 ccaacatcac tagctaccat caaaagattg acttctcatc ttactcgatt gaaaccaaat 121 taacataggg tttttattta aataaaagtt taaccttctt tttaaaaaat tgttcatagt 181 gtcatgtcag aacaagagct acaaatcaca catagcatgc ataagcggag ctatgatgag 241 tggtattgtt ttgttcgtca cttgtcactc ttttccaaca cataatcccg acaacaacgt 301 aagagcatct ctctctctcc acacacactc atgcatgcat gcattcttac acgtgattgc 361 catgcaaatc tcctttctca cctataaata caaaccaacc cttcactaca ctcttcactc 421 aaaccaaaac aagaaaacat acacaaatag caaaacggta ccaacaaaac tagtatggat 481 catgcgaccc tcgccatgat cctcgcgatc tggttcatca gcttccactt catcaagctg 541 ctgttctccc agcagaccac caagctgctt ccgccaggac caaagccgct tccgatcatc 601 ggcaacatcc ttgaggtggg caagaagccg catcggtcct tcgccaacct cgccaagatt 661 cacggcccac tcatttccct cagactcggc tctgtgacca ccatcgttgt gtcctctgcc 721 gacgtggcca aagagatgtt cctcaagaag gatcacccgc tctccaaccg cacgatcccg 781 aatagtgtta cagccggcga ccaccacaag ctcaccatgt cttggctccc ggtgtctccg 841 aagtggcgca acttccgcaa gattaccgcc gtgcatctgc tctccccaca gagactcgat 901 gcctgccaga cattcaggca cgccaaggtg cagcagctct acgagtacgt tcaagagtgc 961 gcccagaaag gccaggccgt ggatattggc aaggccgcct ttacgaccag cctcaacctc 1021 ctcagcaagc tgttcttcag cgtcgagctg gcgcaccaca agtcccatac cagccaagag 1081 ttcaaagagc tgatctggaa catcatggaa gatataggca agccgaacta cgccgactac 1141 ttcccgattc tcggctgcgt tgacccatct ggcattagaa gaaggctcgc ctgctccttc 1201 gacaagctga tcgccgtgtt ccagggcatc atctgcgaga gactcgcccc agattcctcc 1261 accacaacta ccaccaccac cgacgacgtg ctcgatgtgc tcctccagct gttcaagcag 1321 aacgagctga cgatgggcga gatcaaccac ctcctcgtgg acatcttcga cgccggcacc 1381 gataccacat cctccacatt cgagtgggtg atgaccgagc tgatccgcaa tccagagatg 1441 atggaaaagg cccaagagga aatcaagcag gtcctcggca aggacaagca gatccaagag 1501 tccgacatca tcaacctgcc gtacctccag gcgatcatca aagagacact ccgcctccat 1561 ccgccgaccg tgttcttgct cccaagaaag gccgacaccg atgtcgagct gtacggctac 1621 atcgtgccga aggatgccca gatcctcgtg aacctctggg ccattggcag ggacccaaac 1681 gcctggcaga acgccgatat tttcagccca gagcgcttca tcggctgcga gatcgatgtt 1741 aagggccgcg atttcggcct ccttccattt ggcgctggcc gcagaatttg cccaggcatg 1801 aatctcgcca tcaggatgct caccctcatg ctcgccacac tcctccagtt cttcaactgg 1861 aagctcgaag gcgacatctc cccgaaggac ctcgacatgg acgagaagtt cggcattgcg 1921 ctccaaaaga ccaagccgct caagctcatc ccgattccgc gctacggtag cggagctacc 1981 aattttagcc tccttaagca ggcaggtgat gtagaagaga accccgggcc tatgaagatg 2041 atgaacggcg aggacgccaa cgaccagatg atcaaagagt ccttcttcat cacccacggc 2101 aacccgatcc tcaccgtcga ggatacacat ccgctcaggc cgttcttcga gacatggcgc 2161 gagaagattt tctccaagaa gccgaaggcc atcctcatca tctccggcca ctgggagaca 2221 gtgaagccaa ccgtgaacgc cgtgcacatc aacgacacca tccacgactt cgacgactac 2281 ccagccgcca tgtaccagtt caagtaccca gctccaggcg agccagagct tgcgagaaag 2341 gtggaagaga tcctcaagaa gtccgggttc gagacagccg agacagacca aaagaggggc 2401 cttgatcacg gcgcctgggt tccactcatg ctcatgtatc cagaggcgga catcccggtg 2461 tgccagctct cagttcagcc acatctcgac ggcacctacc actacaatct cggcagagcc 2521 ctcgcgccgc tcaagaatga tggcgtgctc attattggct ccggcagcgc cacacatcca 2581 ctcgatgaga caccgcacta cttcgatggt gttgcccctt gggccgctgc cttcgattct 2641 tggcttagga aggccctcat caacggccgc ttcgaggaag tgaacatcta cgagagcaag 2701 gccccgaact ggaagctcgc ccatccattt ccagagcact tctacccgct ccacgttgtg 2761 ctcggcgctg ctggtgaaaa gtggaaggcc gagctgatcc actcctcctg ggatcatggc 2821 acactttgcc acggctccta caagttcacc tccgccggat ccggagcaac caactttagc 2881 ctgctcaagc aagcaggaga tgttgaggaa aatcctggcc ccatgaccgc catcaagatg 2941 aacaccaacg gcgagggcga gacacagcac atcctcatga tcccgttcat ggcgcagggc 3001 cacctcaggc catttctcga actcgccatg ttcctctaca agcgctccca cgtgatcatc 3061 accctgctca caactccgct caacgccggc ttcctcaggc acctccttca ccaccattcc 3121 tactcctcca gcggcatcag gatcgtcgag ctgccattca actccaccaa ccacggactc 3181 ccaccgggca tcgagaacac cgataagctc acactcccgc tcgtggtgtc cctcttccat 3241 tccaccatca gcctcgatcc gcacctccgc gattacatct ccaggcattt cagcccagcc 3301 aggccaccac tctgcgtgat ccatgatgtg ttcctcggct gggttgacca ggtggccaag 3361 gatgtgggct ctacaggcgt ggtgttcaca acaggcggcg cttatggcac atccgcctac 3421 gtgtccatct ggaacgatct cccgcaccag aactactccg acgaccaaga gttcccgctg 3481 ccaggcttcc cagagaacca taagttccgc aggtcccagc tccatcggtt cctcagatat 3541 gccgacggct ccgacgattg gtccaagtat ttccagccgc agctccgcca gtccatgaag 3601 tcttttggct ggctctgcaa ctccgtggaa gagatcgaga cactcggctt ctccatcctc 3661 cgcaactaca ccaagctgcc gatctggggc atcggcccac ttattgcttc cccagtgcag 3721 cactcctcct ccgacaacaa ttcaacaggc gccgagttcg tgcagtggct cagcctcaaa 3781 gagccggact ccgtcctcta catctccttc ggctcccaga acacgatcag cccgacgcag 3841 atgatggaac tcgctgctgg ccttgagtcc tccgagaagc cattcctctg ggtgatcaga 3901 gccccgttcg gcttcgacat caacgaagag atgcgcccag agtggctgcc agagggcttt 3961 gaggaacgca tgaaggtgaa gaaacagggc aagctcgtgt acaagctcgg cccgcagctt 4021 gagatcctca accatgaatc catcggcggc tttctcaccc actgcggatg gaacagcatc 4081 cttgagtctc ttcgcgaggg cgttccgatg cttggatggc cacttgctgc cgagcaggcc 4141 tacaacctca agtacctcga agatgagatg ggcgtcgcgg ttgagcttgc tagaggcctc 4201 gaaggcgaga tctccaaaga gaaggtcaag cgcatcgtcg agatgatcct tgagcgcaac 4261 gagggctcca aaggctggga gatgaagaat cgcgccgtgg aaatgggcaa aaagctcaag 4321 gacgccgtga acgaggaaaa agagctgaag ggctcctccg tgaaggcgat cgacgatttc 4381 ctcgacgccg tcatgcaggc caaacttgag ccaagcctcc agtgatagtg aatatgaaga 4441 tgaagatgaa atatttggtg tgtcaaataa aaaggttgtg tgcttaagtt tgtgtttttt 4501 tcttggcttg ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaacatct 4561 cattataatg aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt 4621 ctccagcata taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa 4681 gctagaccta actgagtaag ctagcttgtt cgagtattat ggcattggga aaactgtttt 4741 tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga 4801 actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc 4861 tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag 4921 agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga 4981 agttaatatg aggagtaaaa catcccaaac aagcttggaa actgaaggcg ggaaacgaca 5041 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga 5101 caagccgttt tacgtttgga actgacagaa ccgcaacgat tgaaggagcc actcagccgc 5161 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa 5221 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt 5281 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc 5341 tgcaccggat cccctagaat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt 5401 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct 5461 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc 5521 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt 5581 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt 5641 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg 5701 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc 5761 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt 5821 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc 5881 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc 5941 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc 6001 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc 6061 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg 6121 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc 6181 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac 6241 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg 6301 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc 6361 actcgtccga gggcaaagga atagcgatcg ttcaaacatt tggcaataaa gtttcttaag 6421 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 6481 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag 6541 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga 6601 taaattatcg cgcgcggtgt catctatgtt actagatcgg ggaattgatc ccccctcgac 6661 agcttgcatg ccagcttggg ctgcaggtcg acgcgtcaat tgatatcgat ccaggcctcc 6721 cagctttcgt ccgtatcatc ggtttcgaca acgttcgtca agttcaatgc atcagtttca 6781 ttgcccacac accagaatcc tactaagttt gagtattatg gcattggaaa agctgttttc 6841 ttctatcatt tgttctgctt gtaatttact gtgttctttc agtttttgtt ttcggacatc 6901 aaaatgcaaa tggatggata agagttaata aatgatatgg tccttttgtt cattctcaaa 6961 ttattattat ctgttgtttt tactttaatg ggttgaattt aagtaagaaa ggaactaaca 7021 gtgtgatatt aaggtgcaat gttagacata taaaacagtc tttcacctct ctttggttat 7081 gtcttgaatt ggtttgtttc ttcacttatc tgtgtaatca agtttactat gagtctatga 7141 tcaagtaatt atgcaatcaa gttaagtaca gtataggctt tttgtgtcga gggggtaccg 7201 agtcgaggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 7261 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 7321 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgggtac cgagctcgaa 7381 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta 7441 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac 7501 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc 7561 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga 7621 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat 7681 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata 7741 agcctcgttc ggttcgtaag ctgtaatgca agtagcgtaa ctgccgtcac gcaactggtc 7801 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttcttg 7861 ttatgacatg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt 7921 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc 7981 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac 8041 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt 8101 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta 8161 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa 8221 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc 8281 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc 8341 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta 8401 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac 8461 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat 8521 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca 8581 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa 8641 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg 8701 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg 8761 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc 8821 cgacgccgct tcgccggcgt taactcaagc gattagatgc actaagcaca taattgctca 8881 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta 8941 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac 9001 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 9061 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 9121 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 9181 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 9241 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 9301 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 9361 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 9421 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 9481 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 9541 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 9601 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 9661 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 9721 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 9781 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat 9841 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 9901 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac 9961 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 10021 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 10081 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct 10141 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc 10201 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct 10261 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta 10321 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata 10381 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg 10441 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga 10501 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag 10561 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca 10621 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg 10681 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc 10741 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa 10801 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc 10861 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc 10921 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc 10981 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt 11041 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag 11101 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct 11161 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa 11221 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc 11281 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg 11341 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct 11401 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc 11461 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct 11521 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca 11581 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgtcacg ccgattcctc 11641 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg 11701 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg 11761 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca 11821 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc 11881 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct 11941 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg 12001 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca 12061 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc 12121 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg 12181 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc 12241 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc 12301 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta 12361 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa 12421 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac 12481 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac 12541 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc 12601 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt 12661 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat 12721 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt 12781 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg 12841 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc 12901 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt 12961 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg 13021 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct 13081 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc 13141 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt 13201 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg 13261 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc 13321 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg 13381 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc 13441 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt 13501 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa 13561 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt 13621 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat 13681 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac 13741 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt 13801 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt 13861 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac 13921 gaacggataa accttttcac gcccttttaa atatccgtta ttctaataaa cgctcttttc 13981 tcttag //