LOCUS       Exported               11715 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses nCDA1-BE3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    pJT45_GalL_nCDA1-BE3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Tan J, Zhang F, Karcher D, Bock R
  TITLE     Engineering of high-precision base editors for site-specific single 
            nucleotide replacement.
  JOURNAL   Nat Commun. 2019 Jan 25;10(1):439. doi: 10.1038/s41467-018-08034-8.
  PUBMED    30683865
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..624
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             673..4776
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4789..5037
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5050..5070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5086..5333
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5102..5120)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5351..5370)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5352..5370)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5380..5397)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5380..5396)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5389..5411)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5537..5992
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5624..5643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5834..5855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6293..6700
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6701..7795
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(8289..8308)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8408..8430
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8468..8486)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    8528..9031
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        9068..9172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9173..10033
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9391..9410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10204..10792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10693..10712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10946..10963
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11080..11101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11116..11146
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11154..11170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11159..11181
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11178..11194
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11178..11194
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11213..11233
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11215..11233
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     11642..11665
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     11686..11702
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11686..11702
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atgaccgacg ctgagtacgt gagaatccat gagaagttgg acatctacac gtttaagaaa
       61 cagtttttca acaacaaaaa atccgtgtcg catagatgct acgttctctt tgaattaaaa
      121 cgacggggtg aacgtagagc gtgtttttgg ggctatgctg tgaataaacc acagagcggg
      181 acagaacgtg gcattcacgc cgaaatcttt agcattagaa aagtcgaaga atacctgcgc
      241 gacaaccccg gacaattcac gataaattgg tactcatcct ggagtccttg tgcagattgc
      301 gctgaaaaga tcttagaatg gtataaccag gagctgcggg ggaacggcca cactttgaaa
      361 atctgggctt gcaaactcta ttacgagaaa aatgcgagga atcaaattgg gctgtggaac
      421 ctcagagata acggggttgg gttgaatgta atggtaagtg aacactacca atgttgcagg
      481 aaaatattca tccaatcgtc gcacaatcaa ttgaatgaga atagatggct tgagaagact
      541 ttgaagcgag ctgaaaaacg acggagcgag ttgtccatta tgattcaggt aaaaatactc
      601 cacaccacta agagtcctgc tgtttctggt tctgaaactc caggtacttc tgaatctgct
      661 actccagaat ctatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc
      721 ggttgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc
      781 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg
      841 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
      901 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
      961 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
     1021 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
     1081 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc
     1141 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
     1201 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
     1261 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg
     1321 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
     1381 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
     1441 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac
     1501 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg
     1561 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg
     1621 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag
     1681 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa
     1741 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt
     1801 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa
     1861 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg
     1921 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac
     1981 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc
     2041 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg
     2101 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact
     2161 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag
     2221 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag
     2281 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac
     2341 cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac
     2401 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat
     2461 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt
     2521 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg
     2581 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat
     2641 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga
     2701 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg
     2761 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag
     2821 ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga
     2881 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc
     2941 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac
     3001 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt
     3061 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg
     3121 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac
     3181 gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg
     3241 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc
     3301 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag
     3361 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc
     3421 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat
     3481 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt
     3541 actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga
     3601 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca
     3661 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac
     3721 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac
     3781 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag
     3841 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag
     3901 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa
     3961 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc
     4021 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct
     4081 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa
     4141 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa
     4201 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt
     4261 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt
     4321 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg
     4381 tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag
     4441 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc
     4501 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag
     4561 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc
     4621 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac
     4681 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
     4741 gaaacaagaa tcgacctctc tcagctcggt ggagactctg gtggttcaac taatttatct
     4801 gatattattg aaaaagaaac aggtaaacaa ttagttattc aagaatcaat tttaatgttg
     4861 ccagaagaag ttgaagaagt tattggtaat aaaccagaat ctgatatttt agttcatact
     4921 gcttatgatg aatctactga tgaaaatgtt atgttattga cttcagatgc tccagaatat
     4981 aaaccatggg ctttagttat tcaagattct aatggtgaaa ataaaattaa aatgttgtct
     5041 ggtggttcac ccaagaagaa gaggaaggtg tgatctcttc tcgagtcatg taattagtta
     5101 tgtcacgctt acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta
     5161 gacaacctga agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt
     5221 tatttatatt tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta
     5281 tactgaaaac cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt
     5341 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     5401 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5461 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5521 cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5581 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5641 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5701 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5761 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5821 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5881 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5941 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     6001 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatcgacc ggtcgaggag
     6061 aacttctagt atatctacat acctaatatt attgccttat taaaaatgga atcccaacaa
     6121 ttacatcaaa atccacattc tcttcaaaat caattgtcct gtacttcctt gttcatgtgt
     6181 gttcaaaaac gttatattta taggataatt atactctatt tctcaacaag taattggttg
     6241 tttggccgag cggtctaagg cgcctgattc aagaaatatc ttgaccgcag ttaactgtgg
     6301 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
     6361 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
     6421 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
     6481 agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca
     6541 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
     6601 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc
     6661 agcaatatat atatatatat ttcaaggata taccattcta atgtctgccc ctaagaagat
     6721 cgtcgttttg ccaggtgacc acgttggtca agaaatcaca gccgaagcca ttaaggttct
     6781 taaagctatt tctgatgttc gttccaatgt caagttcgat ttcgaaaatc atttaattgg
     6841 tggtgctgct atcgatgcta caggtgttcc acttccagat gaggcgctgg aagcctccaa
     6901 gaaggctgat gccgttttgt taggtgctgt gggtggtcct aaatggggta ccggtagtgt
     6961 tagacctgaa caaggtttac taaaaatccg taaagaactt caattgtacg ccaacttaag
     7021 accatgtaac tttgcatccg actctctttt agacttatct ccaatcaagc cacaatttgc
     7081 taaaggtact gacttcgttg ttgtcagaga attagtggga ggtatttact ttggtaagag
     7141 aaaggaagac gatggtgatg gtgtcgcttg ggatagtgaa caatacaccg ttccagaagt
     7201 gcaaagaatc acaagaatgg ccgctttcat ggccctacaa catgagccac cattgcctat
     7261 ttggtccttg gataaagcta atgttttggc ctcttcaaga ttatggagaa aaactgtgga
     7321 ggaaaccatc aagaacgaat tccctacatt gaaggttcaa catcaattga ttgattctgc
     7381 cgccatgatc ctagttaaga acccaaccca cctaaatggt attataatca ccagcaacat
     7441 gtttggtgat atcatctccg atgaagcctc cgttatccca ggttccttgg gtttgttgcc
     7501 atctgcgtcc ttggcctctt tgccagacaa gaacaccgca tttggtttgt acgaaccatg
     7561 ccacggttct gctccagatt tgccaaagaa taaggtcaac cctatcgcca ctatcttgtc
     7621 tgctgcaatg atgttgaaat tgtcattgaa cttgcctgaa gaaggtaagg ccattgaaga
     7681 tgcagttaaa aaggttttgg atgcaggtat cagaactggt gatttaggtg gttccaacag
     7741 taccaccgaa gtcggtgatg ctgtcgccga agaagttaag aaaatccttg cttaaaaaga
     7801 ttctcttttt ttatgatatt tgtacataaa ctttataaat gaaattcata atagaaacga
     7861 cacgaaatta caaaatggaa tatgttcata gggtagacga aactatatac gcaatctaca
     7921 tacatttatc aagaaggaga aaaaggagga tgtaaaggaa tacaggtaag caaattgata
     7981 ctaatggctc aacgtgataa ggaaaaagaa ttgcacttta acattaatat tgacaaggag
     8041 gagggcacca cacaaaaagt taggtgtaac agaaaatcat gaaactatga ttcctaattt
     8101 atatattgga ggattttctc taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga
     8161 cctgaccatt tgatggagtt taagtcaata ccttcttgaa ccatttccca taatggtgaa
     8221 agttccctca agaattttac tctgtcagaa acggccttaa cgacgtagtc gatatggtgc
     8281 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     8341 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8401 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     8461 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8521 taggacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg atggaataat
     8581 ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt tagaaagtaa
     8641 ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat
     8701 ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag attaaataga
     8761 tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc
     8821 tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga gcaagataaa
     8881 aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa caaaaactat
     8941 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
     9001 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
     9061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10441 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10501 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10561 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10621 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10681 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10741 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10801 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    10861 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10921 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10981 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    11041 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    11101 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    11161 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    11221 ccctcactaa agggaacaaa agctggagct cgtgacagcc ctccgaagga agactctcct
    11281 ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac
    11341 tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg
    11401 gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga
    11461 taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt
    11521 tgatctatta acggatatat aaatgcaaaa actgcataac cactttaact aatactttca
    11581 acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt
    11641 taatatacct ctatacttta acgtcaagga gaaaaaaccc cggattctag aactagtgga
    11701 tcccccggga aaaaa
//
